| Budget Amount *help |
¥216,320,000 (Direct Cost: ¥166,400,000、Indirect Cost: ¥49,920,000)
Fiscal Year 2013: ¥31,980,000 (Direct Cost: ¥24,600,000、Indirect Cost: ¥7,380,000)
Fiscal Year 2012: ¥57,850,000 (Direct Cost: ¥44,500,000、Indirect Cost: ¥13,350,000)
Fiscal Year 2011: ¥51,870,000 (Direct Cost: ¥39,900,000、Indirect Cost: ¥11,970,000)
Fiscal Year 2010: ¥74,620,000 (Direct Cost: ¥57,400,000、Indirect Cost: ¥17,220,000)
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| Outline of Final Research Achievements |
This work aimed to develop new strategy of small RNA analysis based on the RNA-binding fluorescent ligands in confined nanopores. Toward this end, we focused on the design of fluorescent abasic site (AP site)-bidning ligands (APLs) in RNA or DNA/RNA duplexes. We successfully found useful G, C, A, or U-discriminating candidates targeting the duplexes. Moreover, new class of light-up fluorescent ligands was developed based on the conjugation of APLs with cyanine dyes. On the other hand, we revealed several unique features of confined spaces inside the silica and alumina nanopores. Especially, exothermic reactions such as cyclodextrin inclusion and DNA hybridization were found to be accelerated inside the nanopores under supercooled condition. Finally, we found possible applications to detect dozens of attomole nucleic acids and analyze their sequences with single nucleotide resolution by the binding of APL-cyanine dye conjugates inside the confined nanopores under supercooled conditions.
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