| Project/Area Number |
22228004
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| Research Category |
Grant-in-Aid for Scientific Research (S)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied animal science
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| Research Institution | Tokyo University of Agriculture |
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Principal Investigator |
KONO Tomohiro 東京農業大学, 応用生物科学部, 教授 (80153485)
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| Co-Investigator(Kenkyū-buntansha) |
SOTOMARU Yusuke 広島大学, 自然科学研究支援センター, 教授 (90309352)
SUZUKI Yutaka 東京大学, 新領域形成研究科, 教授 (40323646)
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| Research Collaborator |
KOBAYASHI Hisato 東京農業大学, 応用生物科学部・生物資源ゲノム解析センター, 准教授 (70632727)
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| Project Period (FY) |
2010-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥217,620,000 (Direct Cost: ¥167,400,000、Indirect Cost: ¥50,220,000)
Fiscal Year 2014: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2013: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2012: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2011: ¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000)
Fiscal Year 2010: ¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000)
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| Keywords | DNA メチローム / 生殖系列 / 次世代シークエンサー / トランスクリプトーム / 始原生殖細胞 / 生殖細胞 / リプログラミング / DNAメチローム / 脱メチル化 / エピゲノム / DNAメチル化 / メチローム / イピゲノム |
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| Outline of Final Research Achievements |
To clarify DNA methylation reprogramming and transcriptome in the whole female and male germline lineage in mice, a comprehensive DNA methylation analysis for all cytosine was conducted using a next-generation sequencer. DNA methylation was 30% and 80% in the oocytes and sperm, respectively. After fertilization the level decreased and reached to 18% at the blastocysts, however, in the E7.5 epiblast, the DNA methylation was quickly increased to 73%. In the primordial germ cells, which are differentiated at this stage, demethylation occurred rapidly, and the methylation level deceased to less than 5% in both sexes at E13.5. Subsequently, female- and male-specific DNA methylomes were established independently in the sparmatogonium before birth and during oocyte growth period, respectively. Transcriptome analysis revealed that DNA methylation is a critical regulator of gene expression in germline cells. This is the first report of a complete DNA methylome map of the whole germline in mice.
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| Assessment Rating |
Verification Result (Rating)
A+
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Assessment Rating |
Result (Rating)
A+: Progress in the research exceeds the initial goal. More than expected research results are expected.
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