Budget Amount *help |
¥217,620,000 (Direct Cost: ¥167,400,000、Indirect Cost: ¥50,220,000)
Fiscal Year 2014: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2013: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2012: ¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2011: ¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000)
Fiscal Year 2010: ¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000)
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Outline of Final Research Achievements |
To clarify DNA methylation reprogramming and transcriptome in the whole female and male germline lineage in mice, a comprehensive DNA methylation analysis for all cytosine was conducted using a next-generation sequencer. DNA methylation was 30% and 80% in the oocytes and sperm, respectively. After fertilization the level decreased and reached to 18% at the blastocysts, however, in the E7.5 epiblast, the DNA methylation was quickly increased to 73%. In the primordial germ cells, which are differentiated at this stage, demethylation occurred rapidly, and the methylation level deceased to less than 5% in both sexes at E13.5. Subsequently, female- and male-specific DNA methylomes were established independently in the sparmatogonium before birth and during oocyte growth period, respectively. Transcriptome analysis revealed that DNA methylation is a critical regulator of gene expression in germline cells. This is the first report of a complete DNA methylome map of the whole germline in mice.
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