• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

MENDで拓く遺伝子治療への道:遺伝子の運び屋からナノマシンへ

Research Project

Project/Area Number 22229001
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Drug development chemistry
Research InstitutionHokkaido University

Principal Investigator

原島 秀吉  北海道大学, 薬学研究科(研究院), 教授 (00183567)

Co-Investigator(Kenkyū-buntansha) 山田 勇磨  北海道大学, 薬学研究科(研究院), 助教 (60451431)
中村 孝司  北海道大学, 薬学研究科(研究院), 助教 (20604458)
兵藤 守  北海道大学, 薬学研究科(研究院), 特任助教 (30548186)
馬場 嘉信  名古屋大学, 工学(系)研究科(研究院), 教授 (30183916)
篠原 康雄  徳島大学, 疾患ゲノム研究センター, 教授 (60226157)
小暮 健太朗 (小暮 健太郎)  京都薬科大学, 薬学部, 教授 (70262540)
紙谷 浩之  愛媛大学, 理工学研究科, 教授 (10204629)
渡慶次 学  北海道大学, 工学(系)研究科(研究院), 教授 (60311437)
松尾 保孝  北海道大学, 電子科学研究所, 准教授 (90374652)
秋田 英万  北海道大学, 大学院・薬学研究院, 准教授 (80344472)
Project Period (FY) 2010-05-31 – 2014-03-31
Project Status Declined (Fiscal Year 2013)
Budget Amount *help
¥184,080,000 (Direct Cost: ¥141,600,000、Indirect Cost: ¥42,480,000)
Fiscal Year 2013: ¥38,090,000 (Direct Cost: ¥29,300,000、Indirect Cost: ¥8,790,000)
Fiscal Year 2012: ¥43,550,000 (Direct Cost: ¥33,500,000、Indirect Cost: ¥10,050,000)
Fiscal Year 2011: ¥47,450,000 (Direct Cost: ¥36,500,000、Indirect Cost: ¥10,950,000)
Fiscal Year 2010: ¥54,990,000 (Direct Cost: ¥42,300,000、Indirect Cost: ¥12,690,000)
Keywordsナノマシン / MEND / マイクロ流路 / 核内動態制御 / ミトコンドリア / ナノパッケージング / pH-感受性カチオニック脂質 / 還元環境応答性 / ミトコンドリア送達 / ハイドロダイナミクス法 / 自己活性化システム / バイセル / 細胞内動態制御 / パッケージング / 核送達 / KALA / 機能性核酸
Research Abstract

パッチワーク法による新しいMEND構築方法を確立し一枚膜でコア粒子をパッケージする新しい方法を開発し、平均粒子径が25nm程度でゼータ電位が-40mV程度の構造体を得ることに成功し、P-MENDと命名した。D-MEND法と組み合わせることでPD- MEND(3枚膜構造)を構築したところ、市販の試薬より高い活性を示した。マイクロ流体デバイスを開発し、最小粒径30nmのMENDを作製することに成功した。一方でマイクロ流路作製についての検討し、ナノインプリント手法によりガラスマイクロ流路の量産が可能であることをしめした。また、シースフローと電場を利用したマイクロフリーフロー電気泳動デバイスの構造・分離条件の最適化を行い、MEND精製に適用することでMENDの定性的かつ定量的評価法を確立した。KALAペプチドを導入した脂質膜封入型ナノ粒子にがん抗原等をコードした遺伝子を封入することで、抗腫瘍活性を得ることに成功した。また、本粒子は細胞に対し免疫活性化効果を有することを示し、その機構に細胞質内DNAセンサーが関与することを示唆する結果を得た。酵素耐性RNAアプタマーの単離に世界で始めて成功した。Mt移行性ペプチドを搭載したMTS-MITO-Porterを構築し、生細胞内での選択的Mt送達システムを開発し、MTS-MITO-Porterは従来型MITO-Porterと比較してミトコンドリア移行能が飛躍的に上昇した。GAL4とVP16の融合蛋白質を用いる自己活性化システムを構築し、reporterプラスミドDNAの単独投与に比べて数十倍にルシフェラーゼ活性が上昇した。アンチセンスRNA (ASO)をMITO-Porterを用いて細胞Mtに送達し、ミトコンドリアmRNAを40%抑制し、さらに標的mRNAがコードする内因性Mtタンパク質の発現量が低下した。

Current Status of Research Progress
Reason

翌年度、交付申請を辞退するため、記入しない。

Strategy for Future Research Activity

翌年度、交付申請を辞退するため、記入しない。

Report

(3 results)
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (56 results)

All 2013 2012 2011 2010 Other

All Journal Article (31 results) (of which Peer Reviewed: 31 results) Presentation (15 results) (of which Invited: 3 results) Book (5 results) Patent(Industrial Property Rights) (5 results) (of which Overseas: 2 results)

  • [Journal Article] Particle tracking analysis for the intracellular trafficking of nanoparticles modified with African swine fever virus protein p54-derived peptide2013

    • Author(s)
      Akita H, Enoto K, Tanaka H, Harashima H
    • Journal Title

      Molecular Therapy

      Volume: 21(2) Issue: 2 Pages: 309-317

    • DOI

      10.1038/mt.2012.235

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The transfection activity of R8-modified nanoparticles and siRNA condensation using pH sensitive stearylated-octahistidine2013

    • Author(s)
      Toriyabe N, Hayashi Y, Harashima H
    • Journal Title

      Biomaterials

      Volume: 34(4) Issue: 6 Pages: 1337-1343

    • DOI

      10.1016/j.biomaterials.2012.11.006

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Novel Drug Delivery System consisting of an Antitumor Agent Tocopheryl Succinate2012

    • Author(s)
      Hama S, Utsumi S, Fukuda Y, Nakayama K, Okamura Y, Tsuchiya H, Fukuzawa K, Harashima H, Kogure K
    • Journal Title

      Journal of Control release

      Volume: 161(3) Issue: 3 Pages: 843-851

    • DOI

      10.1016/j.jconrel.2012.05.031

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Multifunctional Envelope-type Nanodevice for Use in Nanomedicine: Concept and Applications2012

    • Author(s)
      Nakamura T, Akita H, Yamada Y, Hatakeyama H, Harashima H
    • Journal Title

      Acc Chem Res

      Volume: 45(7) Issue: 7 Pages: 1113-1121

    • DOI

      10.1021/ar200254s

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The manner in which DNA is packaged with TFAM has an impact on transcription activation and inhibition2012

    • Author(s)
      Furukawa R, Yamada Y, Matsushima Y, Goto Y-I, Harashima H.
    • Journal Title

      FEBS open bio.

      Volume: 2 Issue: 1 Pages: 145-150

    • DOI

      10.1016/j.fob.2012.06.001

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of functionalized double-lamellar multifunctional envelope-type nanodevices using a microfluidic chip with achaotic mixer array2012

    • Author(s)
      Kitazoe K, Park YS, Kaji N, Okamoto Y, Tokeshi M, Kogure K, Harashima H, Baba Y
    • Journal Title

      PLoS One

      Volume: 7(6)

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Delivery of bioactive molecules to the mitochondrial genome using a membrane-fusing, liposome-based carrier, DF-MITO-Porter2012

    • Author(s)
      Y.Yamada, H.Harashima
    • Journal Title

      Biomaterials

      Volume: 33(5) Issue: 5 Pages: 1589-1595

    • DOI

      10.1016/j.biomaterials.2011.10.082

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of the Anchor in Polyethylene Glycol-Lipids on the Transfection Activity of PEGylated Cationic Liposomes Encapsulating DNA2012

    • Author(s)
      Kusumoto K, Akita H, El-Sayed A, Harashima H
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 35 Issue: 4 Pages: 445-448

    • DOI

      10.1248/bpb.35.445

    • NAID

      130001872189

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stearylated INF7 peptide enhances endosomal escape and gene expression of PEGylated nanoparticles both in vitro and in vivo2012

    • Author(s)
      El-Sayed A, Masuda T, Akita H, Harashima H
    • Journal Title

      J Pharm Sci.

      Volume: 101(2) Issue: 2 Pages: 879-882

    • DOI

      10.1002/jps.22807

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Different mechanisms for nanoparticle formation between pDNA and siRNA using Polyrotaxane as the polycation2012

    • Author(s)
      Y.Yamada, M.Hashida, T.Nomura, H.Harashima, Y Yamasaki, K.Kataoka, A.Yamashita, R.Katoono, N.Yui
    • Journal Title

      ChemPhysChem

      Volume: 13(5) Issue: 5 Pages: 1161-1165

    • DOI

      10.1002/cphc.201100800

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Post-nuclear gene delivery events for transgene expression by biocleavable polyrotaxanes2012

    • Author(s)
      Y.Yamada, T.Nomura, H.Harashima, A.Yamashita, N.Yui
    • Journal Title

      Biomaterials

      Volume: 33 Issue: 15 Pages: 3952-3958

    • DOI

      10.1016/j.biomaterials.2012.01.049

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CREB-binding protein transcription activation domain for enhanced transgene expression by a positive feedback system2012

    • Author(s)
      G. Kanda, H. Ochiai, H. Harashima, and H. Kamiya
    • Journal Title

      J. Biotechnol

      Volume: 157(# 1) Issue: 1 Pages: 7-11

    • DOI

      10.1016/j.jbiotec.2011.09.016

    • NAID

      120003811532

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] KALA-modified multi-layered nanoparticles as gene carriers for MHC class-I mediated antigen presentation for a DNA vaccine2011

    • Author(s)
      Shaheen SM, Akita H, Nakamura T, Takayama S, Futaki S, Yamashita A, Katoono R, Yui N, Harashima H
    • Journal Title

      Biomaterials

      Volume: 32(26) Issue: 26 Pages: 6342-6350

    • DOI

      10.1016/j.biomaterials.2011.05.014

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A touch-and-go lipid wrapping technique in microfluidic channels for rapid fabrication of multifunctional envelope-type gene delivery nanodevices2011

    • Author(s)
      Kitazoe K, Wang J, Kaji N, Okamoto Y, Tokeshi M, Kogure K, Harashima H, Baba Y
    • Journal Title

      Lab Chip

      Volume: 11 Issue: 19 Pages: 3256-3262

    • DOI

      10.1039/c1lc20392d

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cell penetrating peptide-mediated systemic siRNA delivery to the liver2011

    • Author(s)
      Hayashi Y, Yamauchi J, Khalil IA, Kajimoto K, Akita H, Harashima H
    • Journal Title

      Int J Pharm

      Volume: 419(1-2) Issue: 1-2 Pages: 308-313

    • DOI

      10.1016/j.ijpharm.2011.07.038

    • NAID

      120003504743

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dual imaging of mRNA and protein production : an investigation of the mechanism of heterogeneity in cationic lipid-mediated transgene expression2011

    • Author(s)
      Akita H, Umetsu Y, Kurihara D, Harashima H
    • Journal Title

      Int J Pharm

      Volume: 415(1-2) Issue: 1-2 Pages: 218-220

    • DOI

      10.1016/j.ijpharm.2011.05.051

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional improvement of an I.RQ-PEG-MEND for delivering genes to the lung2011

    • Author(s)
      Ishitsuka T, Akita H, Harashima H
    • Journal Title

      J Control Release

      Volume: 154(1) Issue: 1 Pages: 77-83

    • DOI

      10.1016/j.jconrel.2011.05.012

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Improving in vivo hepatic transfection activity by controlling intracellular trafficking : the function of GALA and maltotriose2011

    • Author(s)
      Akita H, Masuda T, Nishio T, Niikura K, Ijiro K, Harashima H
    • Journal Title

      Mol Pharm

      Volume: 8(4) Issue: 4 Pages: 1436-1442

    • DOI

      10.1021/mp200189s

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dual function MITO-Porter, a nano carrier integrating both efficient cytoplasmic delivery and mitochondrial macromolecule delivery2011

    • Author(s)
      Yamada Yuma, Ryo Furukawa, Yukari Yasuzaki, Harashima Hideyoshi
    • Journal Title

      Molecular Therapy

      Volume: 19 Issue: 8 Pages: 1449-1456

    • DOI

      10.1038/mt.2011.99

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Noninvasive and efficient transdermal delivery of CpGoligodeoxynucleotide for cancer immunotherapy2011

    • Author(s)
      Kigasawa K, Kajimoto K, Nakamura T, Hama S, Kanamura K, Harashima H, Kogure K
    • Journal Title

      J Control Release

      Volume: 150(3) Issue: 3 Pages: 256-265

    • DOI

      10.1016/j.jconrel.2011.01.018

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correction of Frameshift Mutations with Tailed Duplex DNAs2011

    • Author(s)
      Y. Morita, H. Tsuchiya, H. Harashima, and H. Kamiya
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 34 Issue: 9 Pages: 1465-1468

    • DOI

      10.1248/bpb.34.1465

    • NAID

      130001872441

    • ISSN
      0918-6158, 1347-5215
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of insulator cHS4 on transgene expression from plasmid DNA in a positive feedback system2011

    • Author(s)
      H. Ochiai, H. Harashima, and H. Kamiya
    • Journal Title

      J. Biosci. Bioengng

      Volume: 112(# 5) Issue: 5 Pages: 432-434

    • DOI

      10.1016/j.jbiosc.2011.07.001

    • NAID

      110008761503

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver2011

    • Author(s)
      S. Fukunaga, G. Kanda, J. Tanase, H. Harashima, T. Ohyama, and H. Kamiya
    • Journal Title

      Gene Ther

      Volume: (in press) Issue: 8 Pages: 828-835

    • DOI

      10.1038/gt.2011.127

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Use of highly purified and mixed antibodies for simultaneous detection of multiple protein species released from mitochondria upon induction of the permeability transition2011

    • Author(s)
      Yamamoto T, Ohashi M, Mizutani S, Yoshimura Y, Obana E, Terada H, Shinohara Y
    • Journal Title

      Appl Biochem Biotechnol

      Volume: 163(1) Issue: 1 Pages: 64-70

    • DOI

      10.1007/s12010-010-9016-y

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quantitative evaluation of the effects of cold exposure of rats on the expression levels of ten FABP isoforms in brown adipose tissue2011

    • Author(s)
      Yamamoto T, Yamamoto A, Watanabe M, Kataoka M, Terada H, Shinohara Y
    • Journal Title

      Biotechnol Lett

      Volume: 33(2) Issue: 2 Pages: 237-242

    • DOI

      10.1007/s10529-010-0444-0

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of TMEM45B as a protein clearly showing thermal aggregation in SDS-PAGE gels and dissection of its amino acid sequence responsible for this aggregation2011

    • Author(s)
      Okada N, Yamamoto T, Watanabe M, Yoshimura Y, Obana E, Yamazaki N, Kawazoe K, Shinohara Y, Minakuchi K
    • Journal Title

      Protein Expr Purif

      Volume: 77(1) Issue: 1 Pages: 118-123

    • DOI

      10.1016/j.pep.2011.01.011

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differential effects of cold exposure on gene expression profiles in white versus brown adipose tissue2011

    • Author(s)
      Watanabe M, Yamamoto T, Yamamoto A, Obana E, Niiyama K, Hada T, Ooie T, Kataoka M, Hori T, Houchi H, Shinohara Y
    • Journal Title

      Appl Biochem Biotechnol

      Volume: 165(2) Issue: 2 Pages: 538-547

    • DOI

      10.1007/s12010-011-9273-4

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] S-15176 and its methylated derivative suppress the CsA-insensitive mitochondrial permeability transition and subsequent cytochrome crelease induced by silver ion, and show weak protonophoric activity2011

    • Author(s)
      Kawashima S, Yamamoto T, Horiuchi Y, Fujiwara K, Gouda S, Yoshimura Y, Yamamoto A, Inotani Y, Yamashita K, Kitamura S, Terada H, Kanematsu M, Shishido K, Shinohara Y
    • Journal Title

      Mol Cell Biochem

      Volume: 358(1-2) Issue: 1-2 Pages: 45-51

    • DOI

      10.1007/s11010-011-0919-x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanoparticles for ex vivo siRNA delivery to dendritic cells for cancervaccines : Programmed endosomal escape and dissociation.2010

    • Author(s)
      Akita H, Kogure K, et al
    • Journal Title

      J Control Release.

      Volume: 143 Pages: 311-317

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mitochondrial matrix delivery using a MITO-Porter, a liposome-based carrier that specifies fusion with mitochondrial membranes.2010

    • Author(s)
      Yukari Yasuzaki, Yuma Yamada, et al
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 397 Pages: 181-186

    • NAID

      120002834836

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3.2010

    • Author(s)
      H.Kamiya, H.Goto, et al
    • Journal Title

      Int.J.Pharm.

      Volume: 392 Pages: 249-253

    • NAID

      120002208933

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] ミトコンドリアを標的とした遺伝子治療にむけて2012

    • Author(s)
      山田勇磨
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(北海道)(招待講演)
    • Year and Date
      2012-03-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] ミトコンドリア標的型DDSが拓くミトコンドリア創薬への道2012

    • Author(s)
      山田勇磨
    • Organizer
      日本薬学会第132年会
    • Place of Presentation
      北海道大学(北海道)(招待講演)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Mitochondrial Medicineの創製を目指して2012

    • Author(s)
      山田勇磨
    • Organizer
      若手研究者公開特別シンポジウムミトコンドリアとDDS2
    • Place of Presentation
      北海道大学(北海道)(招待講演)
    • Year and Date
      2012-01-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 多機能性エンベロープ型ナノ構造体の創製とナノメディシンへの展開2011

    • Author(s)
      原島秀吉
    • Organizer
      第40回日本免疫学会学術集会
    • Place of Presentation
      幕張メッセ(千葉)(招待講演)
    • Year and Date
      2011-11-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] Multifunctional Envelope-type Nano Device for Non-viral Gene Delivery : Concept and Application for Nanomedicine2011

    • Author(s)
      Hideyoshi Harashima
    • Organizer
      Joint Meeting of the Austrian and German Pharmaceutical Sciences
    • Place of Presentation
      University of Innsbruck (Austria)(招待講演)
    • Year and Date
      2011-09-23
    • Related Report
      2011 Annual Research Report
  • [Presentation] Multifunctional Envelope-type Nano Device for Non-viral Gene Delivery2011

    • Author(s)
      Hideyoshi Harashima
    • Organizer
      The 3rd Asian Biomaterials Congress
    • Place of Presentation
      BEXCO Busan (Korea)(招待講演)
    • Year and Date
      2011-09-19
    • Related Report
      2011 Annual Research Report
  • [Presentation] バイオイメージングで可視化するナノメディシンの世界~多機能性エンベロープ型ナノ構造体が拓く遺伝子治療への道~2011

    • Author(s)
      原島秀吉
    • Organizer
      第20回日本バイオイメージング学会学術集会
    • Place of Presentation
      千歳科学技術大学(北海道)(招待講演)
    • Year and Date
      2011-09-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] プラスミドDNAの核内動態:その制御に向けて2011

    • Author(s)
      紙谷浩之
    • Organizer
      遺伝子・デリバリー研究会第11回夏期セミナー
    • Place of Presentation
      高知三翠円ホテル(高知)(招待講演)
    • Year and Date
      2011-08-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] DDS製剤の臨床応用への課題、DDS製剤の臨床応用を目指して2011

    • Author(s)
      原島秀吉
    • Organizer
      日本薬剤学会第26年会
    • Place of Presentation
      タワーホール船堀(東京)(招待講演)
    • Year and Date
      2011-05-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] An innovative strategy for mitochondrial drug delivery using MITO-Porter, a liposome based nano-device that specifies fusion with mitochondrial membranes2011

    • Author(s)
      Yuma Yamada
    • Organizer
      26nd APSTJ Annual Meeting, Conference on Pharmaceutical Technology
    • Place of Presentation
      Tower Hall Funabori(Tokyo)(招待講演)
    • Year and Date
      2011-05-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] Controlled Intracellular trafficking of Non-viral Vectors2011

    • Author(s)
      Hideyoshi Harashima
    • Organizer
      The 14th Annual Meeting of ASGCT Scientific Symposium Gene & Cell Therapy Around the Globe : A Showcase of Asia
    • Place of Presentation
      Washington State Convention & Trade Center (USA)(招待講演)
    • Year and Date
      2011-05-21
    • Related Report
      2011 Annual Research Report
  • [Presentation] 多機能性エンベロープ型ナノ構造体による次世代医薬への新たな展開2010

    • Author(s)
      原島秀吉
    • Organizer
      日本薬剤学会第25年会
    • Place of Presentation
      徳島 招待講演
    • Year and Date
      2010-05-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] Multifunctional Envelope-type Nano Device as gene delivery system for Nanomedicine

    • Author(s)
      Hideyoshi Harashima
    • Organizer
      The 10th France - Japan DDS Symposium
    • Place of Presentation
      Grande Hotel de Bordeaux, Bordeaux, France
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Breakthrough Technologies in Gene Delivery for Nanomedicines

    • Author(s)
      Hideyoshi Harashima
    • Organizer
      FIP Centennial, 2012
    • Place of Presentation
      Amsterdam RAI, Amsterdam, The Netherland
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Multifunctional Envelope-type Nano Device for Non-viral Gene Delivery

    • Author(s)
      Hideyoshi Harashima
    • Organizer
      IBS 2012 15th International Biotechnology Symposium and Exhibition
    • Place of Presentation
      EXCO Daegu, Korea
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] Delivery of Nucleic Acids and Gene Delivery2011

    • Author(s)
      H.Akita, H.Hatakeyama, I.A.Khalil, Y.Yamada, H.Harashima
    • Total Pages
      34
    • Publisher
      Elsevier
    • Related Report
      2011 Annual Research Report
  • [Book] バイオテクノロジーシリーズ蛍光イメージング/MRIプローブの開発2011

    • Author(s)
      秋田英万、山田勇磨,中村孝司、畠山浩人、林泰弘、梶本和昭、原島秀吉
    • Total Pages
      10
    • Publisher
      CMC出版
    • Related Report
      2011 Annual Research Report
  • [Book] ペプチド・タンパク性医薬品の新規DDS製剤の開発と応用-遺伝子医学MOOK別冊2011

    • Author(s)
      山田勇磨、中村孝司、原島秀吉
    • Total Pages
      8
    • Publisher
      メディカルドゥ
    • Related Report
      2011 Annual Research Report
  • [Book] Application of a fusiogenic peptide GALA for intracellular delivery2011

    • Author(s)
      Nakase I, Kogure K, Harashima H, Futaki S
    • Total Pages
      10
    • Publisher
      Methods Mol Biol
    • Related Report
      2011 Annual Research Report
  • [Book] Volkmar Weissig and Gerard G.M. D'Souza Wiley A John Wiley & Sons, Inc., Publication2010

    • Author(s)
      IA Khalil, Y.Yamada, et al
    • Total Pages
      31
    • Publisher
      "Chapter 25 Uptake Pathways Dependent Intracellular Trafficking of DNA Carrying Nanodelivery System" Organelle-Specific Pharmaceutical Nanotechnology
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 結合剤およびその利用2012

    • Inventor(s)
      原島秀吉、林泰弘、秋田英万、鳥谷部尚之、富樫亮平
    • Industrial Property Rights Holder
      原島秀吉、林泰弘、秋田英万、鳥谷部尚之、富樫亮平
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2012-174272
    • Filing Date
      2012-08-06
    • Related Report
      2012 Annual Research Report
  • [Patent(Industrial Property Rights)] 脂質膜構造体、脂質膜構造体の製造方法および1の目的物質を1枚の脂質膜で封入する方法2012

    • Inventor(s)
      原島秀吉、山田勇磨、鈴木亮佑
    • Industrial Property Rights Holder
      原島秀吉、山田勇磨、鈴木亮佑
    • Filing Date
      2012-02-24
    • Related Report
      2011 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 核内移行性を有する脂質膜構造体2011

    • Inventor(s)
      原島秀吉, 秋田英万、シャリフモハメドシャヒーン、中村孝司、石井聡一郎、二木史朗
    • Industrial Property Rights Holder
      原島秀吉,秋田英万、シャリフモハメドシャヒーン、中村孝司、石井聡一郎、二木史朗
    • Filing Date
      2011-04-20
    • Related Report
      2011 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 細胞内動態を改善したカチオン性脂質2011

    • Inventor(s)
      丹下耕太、新井将也、久保和弘、秋田英万、原島秀吉、畠山浩人、石破諒平、鵜川真実
    • Industrial Property Rights Holder
      丹下耕太、新井将也、久保和弘、秋田英万、原島秀吉、畠山浩人、石破諒平、鵜川真実
    • Industrial Property Number
      2011-252309
    • Filing Date
      2011-11-18
    • Related Report
      2011 Annual Research Report
  • [Patent(Industrial Property Rights)] 核内移行性を有する脂質膜構造体2010

    • Inventor(s)
      原島秀吉
    • Industrial Property Rights Holder
      国立大学法人北海道大学国立大学法人京都大学
    • Filing Date
      2010-04-21
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi