Exhaustive prediction of microRNAs, snoRNAs and their targets
| Project/Area Number |
22240031
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioinformatics/Life informatics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ASAI Kiyoshi 東京大学, 新領域創成科学研究科, 教授 (30356357)
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| Co-Investigator(Kenkyū-buntansha) |
KIRYU Hisanori 東京大学, 大学院新領域創成科学研究科, 准教授 (80415778)
HIROSE Tetsurou 北海道大学, 遺伝子病制御研究所, 教授 (30273220)
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| Co-Investigator(Renkei-kenkyūsha) |
SATO Kengo 慶應義塾大学, 理工学部, 講師 (20365472)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥46,410,000 (Direct Cost: ¥35,700,000、Indirect Cost: ¥10,710,000)
Fiscal Year 2012: ¥15,340,000 (Direct Cost: ¥11,800,000、Indirect Cost: ¥3,540,000)
Fiscal Year 2011: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2010: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
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| Keywords | バイオインフォマティクス / 機能性RNA / RNA / snoRNA / マイクロRNA / 確率モデル / RNA-RNA相互作用 |
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| Research Abstract |
The following is the main results of this project. (a) We developed a microRNA finding tool, which combines structural and evolutionary features of microRNAs. We showed that our tool outperforms previous methods with less false positives. (b) We developed an algorithm for computing secondary structural accessibilities of RNA sequences. We were able to detect the evolutionary constraints around the target sites of microRNAs. (c) We developed a tool to calculate the changes of free energies and entropies of RNA secondary structures in response to mutations. We applied our method to mitochondrial tRNAs and showed that the increase of structural fluctuation caused by mutations correlated with disease susceptibility. (d) We developed a tool to predict potential secondary structural contexts for each base of an RNA sequence. We applied our method to binding sites of RNA-binding proteins and detected the structural specificities of the proteins.
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Report
(4 results)
Research Products
(40 results)
