• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Deciphering the role of protrudin regulating neuronal vesicular transport

Research Project

Project/Area Number 22240038
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Nerve anatomy/Neuropathology
Research InstitutionKyushu University

Principal Investigator

NAKAYAMA Keiichi  九州大学, 生体防御医学研究所, 教授 (80291508)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥49,920,000 (Direct Cost: ¥38,400,000、Indirect Cost: ¥11,520,000)
Fiscal Year 2012: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2010: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Keywords神経 / 細胞内輸送 / 低分子Gタンパク質 / シナプス / 高次脳機能 / 低分子GTP結合タンパク質
Research Abstract

We investigated the structure-function relationship of protrudin that regulates intracellular vesicle transport in neurons. Protrudin interacts with Rab11 through the RBD11 domain, with VAP and KIF5 through the FFAT motif, and sulfatide and PI(5)P through the atypical FYVE domain. We also created mice deficient in protrudin and found that anatomical and physiological alterations in the mutant mice, suggesting that protrudin contributes to vesicular trafficking in neuronal cells

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (49 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (25 results) (of which Peer Reviewed: 9 results) Presentation (12 results) (of which Invited: 3 results) Book (7 results) Remarks (4 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Ablation of fbxw7 eliminates leukemia-initiating cells by preventing quiescence2013

    • Author(s)
      Takeishi, S., Matsumoto, A., Onoyama, I., Naka, K., Hirao, A., Nakayama, K. I
    • Journal Title

      Cancer Cell

      Volume: 23 Pages: 347-361

    • Related Report
      2012 Final Research Report
  • [Journal Article] Regulation of c-Myc ubiquitination controls chronic myelogenous leukemia initiation and progression2013

    • Author(s)
      Reavie, L., Buckley, S. M., Loizou, E., Takeishi, S., Aranda-Orgilles, B., Ndiaye-Lobry, D., Abdel-Wahab, O., Ibrahim, S., Nakayama, K. I., Aifantis, I
    • Journal Title

      Cancer Cell

      Volume: 23 Pages: 362-375

    • Related Report
      2012 Final Research Report
  • [Journal Article] FBXL21 regulates oscillation of the circadian clock through ubiquitination and stabilization of cryptochromes2013

    • Author(s)
      Hirano, A., Yumimoto, K., Tsunematsu, R., Matsumoto, M., Oyama, M., Kozuka-Hata, H., Nakagawa, T., Lanjakornsiripan, D., *Nakayama, K. I., Fukada, Y. (Co-corresponding authors)
    • Journal Title

      Cell

      Volume: 152 Pages: 1106-1118

    • Related Report
      2012 Final Research Report
  • [Journal Article] Selective escape of proteins from the mitochondria during mitophagy2013

    • Author(s)
      Saita, S., Shirane, M., Nakayama, K. I.
    • Journal Title

      Nature Commun.

      Volume: 4 Pages: 1410-1410

    • Related Report
      2012 Final Research Report
  • [Journal Article] Selective escape of proteins from mitochondria during mitophagy2013

    • Author(s)
      Saita, et al
    • Journal Title

      Nature Communications

      Volume: 29(web) Issue: 1 Pages: 1410-1423

    • DOI

      10.1038/ncomms2400

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ablation of fbxw7 eliminates leukemia-initiating cells by preventing quiescence.2013

    • Author(s)
      Takeishi S
    • Journal Title

      Cancer Cell

      Volume: 23 Issue: 3 Pages: 347-361

    • DOI

      10.1016/j.ccr.2013.01.026

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] FBXL21 Regulates Oscillation of the Circadian Clock through Ubiquitination and Stabilization of Cryptochromes2013

    • Author(s)
      Arisa Hirano, Kanae Yumimoto, Ryosuke Tsunematsu, Masaki Matsumoto, Masaaki Oyama, Hiroko Kozuka-Hata, Tomoki Nakagawa, Darin Lanjakornsiripan, Keiichi I. Nakayama, and Yoshitaka Fukada
    • Journal Title

      Cell

      Volume: 152 Issue: 5 Pages: 1106-1118

    • DOI

      10.1016/j.cell.2013.01.054

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of c-Myc ubiquitination controls chronic myelogenous leukemia initiation and progression.2013

    • Author(s)
      Reavie L
    • Journal Title

      Cancer Cell

      Volume: 23 Issue: 3 Pages: 362-375

    • DOI

      10.1016/j.ccr.2013.01.025

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] UPS delivers pluripotency2012

    • Author(s)
      Okita, Y., Nakayama, K. I.
    • Journal Title

      Cell Stem Cell

      Volume: 11 Pages: 728-730

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
  • [Journal Article] The Skp2-SCF E3 ligase regulates Akt ubiquitination, glycolysis, herceptin sensitivity, and tumorigenesis2012

    • Author(s)
      Chan, C. H., Li, C. F., Yang, W. L., Gao, Y., Lee, S. W., Feng, Z., Huang, H. Y., Tsai, K. K., Flores, L. G., Shao, Y., Hazle, J. D., Yu, D., Wei, W., Sarbassov, D., Hung, M. C., Nakayama, K. I., Lin, H. K.
    • Journal Title

      Cell

      Volume: 149 Pages: 1098-1111

    • Related Report
      2012 Final Research Report
  • [Journal Article] The Skp2-SCF E3 ligase regulates Akt ubiquitination, glycolysis, Herceptin sensitivity and tumorigenesis2012

    • Author(s)
      Chan CH.,Li CF., Yang WL., Gao Y., Lee SW., Feng Z., Huang HY., Tsai KKC., Flores LG., Shao Y., Hazle JD., Yu D., Wei W., Sarbassov D., Hung MC., Nakayama KL., Lin HK.
    • Journal Title

      Cell

      Volume: (印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] p57 is required for quiescence and maintenance of adult hematopoietic stem cells2011

    • Author(s)
      Matsumoto, A., Takeishi, S., Kanie, T., Susaki, E., Onoyama, I., Tateishi, Y., Nakayama, K., Nakayama, K. I.
    • Journal Title

      Cell Stem Cell

      Volume: 9 Pages: 262-271

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
  • [Journal Article] The FBXL5-IRP2 axis is integral to control of iron metabolism in vivo2011

    • Author(s)
      Moroishi, T., Nishiyama, M., Takeda, Y., Iwai, K., Nakayama, K. I.
    • Journal Title

      Cell Metab.

      Volume: 14 Pages: 339-351

    • Related Report
      2012 Final Research Report
  • [Journal Article] Protrudin serves as an adaptor molecule that connects KIF5 and its cargoes in vesicular transport during process formation2011

    • Author(s)
      Matsuzaki, F., Shirane, M., Matsumoto, M., Nakayama, K. I.
    • Journal Title

      Mol. Biol. Cell

      Volume: 22 Pages: 4602-4620

    • Related Report
      2012 Final Research Report
  • [Journal Article] p57Kip2 and p27Kip1 cooperate to maintain hematopoietic stem cell quiescence through interactions with Hsc702011

    • Author(s)
      Zou, P., Yoshihara, H., Hosokawa, K., Tai, I., Shinmyozu, K., Tsukahara, F., Maru, Y., Nakayama, K., Nakayama, K. I., Suda, T.
    • Journal Title

      Cell Stem Cell

      Volume: 9 Pages: 247-261

    • Related Report
      2012 Final Research Report
  • [Journal Article] Deficiency of Rap1-binding protein RAPL causes lymphoproliferative disorders through mislocalization of p27kip12011

    • Author(s)
      Katagiri, K., Ueda, Y., Tomiyama, T., Yasuda, K., Toda, Y., Ikehara, S., Nakayama, K. I., Kinashi, T.
    • Journal Title

      Immunity

      Volume: 34 Pages: 24-38

    • Related Report
      2012 Final Research Report
  • [Journal Article] Fbxw7 regulates lipid metabolism and cell fate decisions in the mouse liver. J. Clin. Inves2011

    • Author(s)
      Onoyama, I., Suzuki, A., Matsumoto, A., Tomita, K., Katagiri, H., Oike, Y., Nakayama, K., Nakayama, K. I
    • Journal Title

      J. Clin. Invest.

      Volume: 121 Pages: 342-354

    • Related Report
      2012 Final Research Report
  • [Journal Article] Spyracopoulos, L., Leng, R. P.: UBE4B promotes Hdm2-mediated degradation of the tumor suppressor p532011

    • Author(s)
      Wu, H., Pomeroy, S. L., Ferreira, M., Teider, N., Mariani, J., Nakayama, K. I., Hatakeyama, S.
    • Journal Title

      Nature Med.

      Volume: 17 Pages: 347-355

    • Related Report
      2012 Final Research Report
  • [Journal Article] SCFFBW7 regulates cellular apoptosis by targeting MCL1 for ubiquitylation and destruction2011

    • Author(s)
      Inuzuka, H., Shaik, S., Onoyama, I., Gao, D., Tseng, A., Maser, R. S., Zhai, B., Wan, L., Gutierrez, A., Lau, A. W., Xiao, Y., Christie, A. L., Aster, J., Settleman, J., Gygi, S. P., Kung, A. L., Look, T., Nakayama, K. I., DePinho, R. A., Wei, W.
    • Journal Title

      Nature

      Volume: 471 Pages: 104-109

    • Related Report
      2012 Final Research Report
  • [Journal Article] The FBXL5-IRP2 axis is integral to control of iron metabolism in vivo2011

    • Author(s)
      Moroishi T., Nishiyama M., Takeda Y., Iwai K., Nakayama KI.
    • Journal Title

      Cell Metabolism

      Volume: 14 Issue: 3 Pages: 339-351

    • DOI

      10.1016/j.cmet.2011.07.011

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fbxw7 regulates lipid metabolism and cell fate decisions in the mouse liver.2011

    • Author(s)
      Onoyama, I., Suzuki, A., Matsumoto, A., Tomita, K., Katagiri, H., Oike, Y., Nakayama, K., Nakayama, K.I.
    • Journal Title

      J.Clin.Invest.

      Volume: 121 Pages: 342-354

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SCF(FBW7) regulates cellular apoptosis by targeting MCL1 for ubiquity lation and destruction.2011

    • Author(s)
      Inuzuka, H., Shaik, S., Onoyama, I., Gao, D., Tseng, A., Maser, R.S., Zhai, B., Wan, L., Gutierrez, A., Lau, A.W., Xiao, Y., Christie, A.L., Aster, J., Settleman, J., Gygi, S.P., Kung, A.L., Look, T., Nakayama, K.I., DePinho, R.A., Wei, W.
    • Journal Title

      Nature

      Volume: 471 Pages: 104-109

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deciphering the transcriptional complex critical for RhoA gene expression and cancer metastasis2010

    • Author(s)
      Chan, C. H., Lee, S. W., Li, C. F., Wang, J., Yang, W. L., Wu, C. Y., Wu, J., Nakayama, K. I., Kang, H. Y., Huang, H. Y., Hung, M. C., Pandolfi, P. P., Lin, H. K.
    • Journal Title

      Nature Cell Biol.

      Volume: 12 Pages: 457-467

    • Related Report
      2012 Final Research Report
  • [Journal Article] KDM7 is a dual demethylase for histone H3 lysines 9 and 27 and functions in brain development2010

    • Author(s)
      Tsukada, Y., Ishitani, T., Nakayama, K. I.
    • Journal Title

      Genes Dev.

      Volume: 24 Pages: 432-437

    • Related Report
      2012 Final Research Report
  • [Journal Article] Increased E4 activity in mice leads to ubiquitin-containing aggregates and degeneration of hypothalamic neurons resulting in obesity2010

    • Author(s)
      Susaki, E., Kaneko-Oshikawa, C., Miyata, K., Tabata, M., Yamada, T., Oike, Y., Katagiri, H., Nakayama, K.I.
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 15538-15547

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Next-generation proteomics uncovers the secret of cancer2013

    • Author(s)
      Nakayama, K. I
    • Organizer
      Organelle Homeostasis Research Center
    • Place of Presentation
      Kick-off Symposium. Fukuoka
    • Year and Date
      2013-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Next-generation proteomics uncovers the secret of cancer2013

    • Author(s)
      Nakayama KI.
    • Organizer
      Organelle Homeostasis Research Center: Kick-off Symposium
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Comprehensive and unbiased identification of substrates for ubiquitin ligases by differential proteomics analysis2012

    • Author(s)
      Nakayama, K. I
    • Organizer
      HUPO 2012 11th World Congress
    • Place of Presentation
      Boston, MA
    • Year and Date
      2012-12-09
    • Related Report
      2012 Final Research Report
  • [Presentation] Cell cycle, metabolism, and signal transduction in cancer revealed by next-generation proteomics2012

    • Author(s)
      Nakayama, K. I
    • Organizer
      cer revealed by next-generation proteomics. 2012 American Association for Cancer Research Annual Meeting
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2012-03-31
    • Related Report
      2012 Final Research Report
  • [Presentation] ユビキチン化による鉄代謝制御機構とその破綻2012

    • Author(s)
      中山 敬一
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 正常幹細胞と癌幹細胞におけるG0期維持機構:"G0期追出し療法"による癌根治の可能性2012

    • Author(s)
      中山 敬一
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Road to absolute quantification of all human proteins by large-scale targeted proteomics2011

    • Author(s)
      中山敬一、松本雅記
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜(招待講演)
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Road to Human Proteome Project: Absolute quantification of all human proteins by large-scale targeted proteomics2011

    • Author(s)
      Nakayama, K. I
    • Organizer
      France-Japan Cancer Meeting
    • Place of Presentation
      France-Japan Cancer Meeting
    • Year and Date
      2011-11-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Road to absolute quantification of all human proteins by large-scale targeted proteomics2011

    • Author(s)
      Nakayama, K. I
    • Organizer
      The 5th International Workshop on Cell Regulations in Division and Arrest
    • Place of Presentation
      Onna, Okinawa, Japan
    • Year and Date
      2011-10-26
    • Related Report
      2012 Final Research Report
  • [Presentation] Comprehensive and unbiased identification of substrates for ubiquitin ligases by differential proteomic analysis2011

    • Author(s)
      Nakayama, K. I., Yumimoto, K., Matsumoto, M., Oyamada, K., Moroishi, T
    • Organizer
      Cold Spring Harbor Symposium "The Ubiquitin Family"
    • Place of Presentation
      Cold Spring Harbor, NY
    • Year and Date
      2011-05-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Comprehensive elucidation of enzyme-substrate relationship by proteomics: Say good-bye to western blotting2010

    • Author(s)
      Nakayama, K. I., Yumimoto, K., Matsumoto, M
    • Organizer
      6th Global-COE International Symposium: NewHorizons for Modern Science - Biology and Medicine at the Crossroads
    • Place of Presentation
      Fukuoka.
    • Year and Date
      2010-08-19
    • Related Report
      2012 Final Research Report
  • [Presentation] Comprehensive elucidation of enzyme-substrate relationship by proteomics : Say good-bye to western blotting.2010

    • Author(s)
      Nakayama, K.I., Yumimoto, K., Matsumoto, M.
    • Organizer
      6th Global-COE International Symposium : New Horizons for Modern Science-Biology and Medicine at the Crossroads
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2010-08-19
    • Related Report
      2010 Annual Research Report
  • [Book] 疾患モデルの作製と利用ーがん2012

    • Author(s)
      沖田康孝, 中山敬一
    • Related Report
      2012 Final Research Report
  • [Book] タンパク質分解. イラストで徹底理解するシグナル伝達キーワード事典2012

    • Author(s)
      中山敬一, 蟹江共春
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Book] ユビキチン. 生物機能モデルと新しいリソース・リサーチツール2011

    • Author(s)
      諸石寿朗, 中山敬一
    • Related Report
      2012 Final Research Report
  • [Book] プロテオミクスが拓く細胞周期研究. 細胞周期フロンティア2010

    • Author(s)
      松本雅記, 中山敬一
    • Publisher
      共立出版
    • Related Report
      2012 Final Research Report
  • [Book] G0期とその周辺:細胞周期の停止と再増殖の分子メカニズム2010

    • Author(s)
      洲崎悦生, 中山敬一
    • Publisher
      共立出版
    • Related Report
      2012 Final Research Report
  • [Book] 創薬・タンパク質研究のためのプロテオミクス解析2010

    • Author(s)
      松本雅記, 中山敬一
    • Publisher
      羊土社
    • Related Report
      2012 Final Research Report
  • [Book] プロテオミクスが拓く細胞周期研究2010

    • Author(s)
      松本雅記、中山敬一
    • Total Pages
      6
    • Publisher
      共立出版,東京
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.bioreg.kyushu-u.ac.jp/saibou/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 九州大学生体防御医学研究所分子医科学分野

    • URL

      http://www.bioreg.kyushu-u.ac.jp/saibou/index.html

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.bioreg.kyushu-u.ac.jp/saibou/index.html

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.bioreg.kyushu-u.ac.jp/saibou/index.html

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] タンパク質の定量方法2009

    • Inventor(s)
      中山敬一・松本雅記
    • Industrial Property Rights Holder
      国立大学法人九州大学
    • Filing Date
      2009-07-17
    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi