| Project/Area Number |
22240044
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fusional basic brain science
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HISHIDA Ryuichi 新潟大学, 脳研究所, 准教授 (90313551)
TOHMI Manabu 新潟大学, 脳研究所, 助教 (10401770)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥51,610,000 (Direct Cost: ¥39,700,000、Indirect Cost: ¥11,910,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2011: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
Fiscal Year 2010: ¥32,370,000 (Direct Cost: ¥24,900,000、Indirect Cost: ¥7,470,000)
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| Keywords | 脳機能イメージング / マクロ共焦点顕微鏡 / 近赤外光 / 活動依存的血流変化 / ヘモグロビン / アルブミン / 赤血球 / ヘマトクリット / 視覚野 / 外側膝状体 / マウス / フラビン蛋白蛍光 / 大脳皮質層構造 / 単一細胞イメージング / 体性感覚野 / マクロ共焦点光学系 / 単一神経細胞イメージング / 近赤外線レーザー / 内側前頭連合野 / 帯状回 |
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| Research Abstract |
We developed a new optical method for imaging neural activity in deep brain structures in mice. We used a macroconfocal microscope in a near-infrared range. Generally,a confocal microscope uses only a part of light passing through a tiny pinhole. Therefore,the diameter of a pinhole was expanded, and sensitivity of our macroconfocal microscopewas strengthened. We labeled red blood cells (RBC) with NIR815, a near-infraredfluorescent probe, and applied the fluorescent RBCs to mice. In these mice,activity-dependent hemodynamic responses elicited by visual stimuli were successfullyimaged in the lateral geniculate body, which located approximately 2.5 mm deep from thecortical surface.
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