Large-scale screening of specific promoters for target neuronal cells and its application to high-order functional analysis in brain
| Project/Area Number |
22241048
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SUZUKI Harukazu 独立行政法人理化学研究所, LSA要素技術開発グループ, プロジェクトディレクター (80333293)
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| Co-Investigator(Kenkyū-buntansha) |
ITOHARA Shigeyoshi 独立行政法人理化学研究所, 行動遺伝学技術開発チーム, チームリーダー (60252524)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥38,480,000 (Direct Cost: ¥29,600,000、Indirect Cost: ¥8,880,000)
Fiscal Year 2012: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2011: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2010: ¥11,180,000 (Direct Cost: ¥8,600,000、Indirect Cost: ¥2,580,000)
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| Keywords | トランスクリプトーム / プロモーター / 視床 / トランスジェニックマウス / 回路遺伝学 / 睡眠 / 脳幹 |
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| Research Abstract |
We have succeeded to develop a technology pipeline to specifically knockdown neuronal cells in any desirable brain regions, which are constructed by integration of the quantitative deepCAGE, the revolutionary technology to identify specific promoters with high accuracy, and the circuit genomics technologies using transgenic mice. Cre transgenic mice, using specific promoters identified by the pipeline, already showed specific recombination in the target regions, indicating usefulness of the mice for the functional analysis of the target brain regions.
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Report
(4 results)
Research Products
(28 results)
