| Project/Area Number |
22241050
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
System genome science
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
MORI Hirotada 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (90182203)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Mamoru 山口大学, 医学研究科, 教授 (30174741)
MATSUNO Hiroshi 山口大学, 理工学研究科, 教授 (10181744)
MAKI Yasushi 大阪医科大学, 医学部, 講師 (60401733)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥39,520,000 (Direct Cost: ¥30,400,000、Indirect Cost: ¥9,120,000)
Fiscal Year 2012: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2011: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2010: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
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| Keywords | molecular bar-code / single gene deletion library / stationary phase / population dynamics / cell death / E. coli / bar-code欠失株 / 長期定常期 / 遺伝的ネットワーク / 細胞死 / モデル化 / クラスター解析 / population変動 / 大腸菌 / 定常期 / bar code欠失株 / バーコード欠失株 / 次世代型シーケンサー / population |
|---|
| Research Abstract |
Organisms in nature spend most of their life time instationary growing phase. Cellular metabolisms in stationary phase are largely different to those in log phase. During long-term stationary phase, organisms might survive against variety of stresses, such as starvation of nutrient. To clear the physiological network during stationary phase, we have constructed the comprehensive bar-coded single gene deletion library of E. coli. Using this new resource, we have developed the method to quantitatively monitor the population dynamics of each of deletion strains during long-term stationary phase.
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