Analysis of cellular network during the death phase of stationary phase.
| Project/Area Number |
22241050
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
System genome science
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
MORI Hirotada 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (90182203)
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| Co-Investigator(Kenkyū-buntansha) |
YAMADA Mamoru 山口大学, 医学研究科, 教授 (30174741)
MATSUNO Hiroshi 山口大学, 理工学研究科, 教授 (10181744)
MAKI Yasushi 大阪医科大学, 医学部, 講師 (60401733)
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|---|
| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥39,520,000 (Direct Cost: ¥30,400,000、Indirect Cost: ¥9,120,000)
Fiscal Year 2012: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2011: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2010: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
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|---|
| Keywords | molecular bar-code / single gene deletion library / stationary phase / population dynamics / cell death / E. coli / bar-code欠失株 / 長期定常期 / 遺伝的ネットワーク / 細胞死 / モデル化 / クラスター解析 / population変動 / 大腸菌 / 定常期 / bar code欠失株 / バーコード欠失株 / 次世代型シーケンサー / population |
|---|
| Research Abstract |
Organisms in nature spend most of their life time instationary growing phase. Cellular metabolisms in stationary phase are largely different to those in log phase. During long-term stationary phase, organisms might survive against variety of stresses, such as starvation of nutrient. To clear the physiological network during stationary phase, we have constructed the comprehensive bar-coded single gene deletion library of E. coli. Using this new resource, we have developed the method to quantitatively monitor the population dynamics of each of deletion strains during long-term stationary phase.
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Report
(4 results)
Research Products
(71 results)
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[Journal Article] Glycogen is the primary source of glucose during the lag phase of E. coli proliferation2012
Author(s)
Yamamotoya T., Dose H., Tian Z., Faure A., Toya Y., Honma M., Igarashi K., Nakahigashi K., Soga T., Mori H., & Matsuno H
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Journal Title
Biochim Biophys Acta
Volume: 1824
Issue: 12
Pages: 1442-1448
DOI
NAID
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Peer Reviewed
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[Presentation] Functional Profiling of the E. coli2012
Author(s)
Y. Otsuka1, R. Takeuchi1, H. Dose1, H-C. Huang2, H-F. Juan3, M. Yamada4, H. Matsuno5, B. L. Wanner6, H. Mori1.
Organizer
Genes in Long-Term Stationary Phase 第6回日本ゲノム微生物学会年会
Place of Presentation
立教大学池袋キャンパス.
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[Book] INTECH2012
Author(s)
Naoko Fujimoto
Total Pages
23
Publisher
Chemical Biology
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