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Development of sustainable sewage treatment technologies utilizing micoribal ecology and its integral modeling

Research Project

Project/Area Number 22246069
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionThe University of Tokyo

Principal Investigator

MINO Takashi  東京大学, 新領域創成科学研究科, 教授 (60166098)

Co-Investigator(Kenkyū-buntansha) SATOH Hiroyasu  東京大学, 大学院新領域創成科学研究科, 准教授 (90251347)
Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥47,840,000 (Direct Cost: ¥36,800,000、Indirect Cost: ¥11,040,000)
Fiscal Year 2013: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2012: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2010: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Keywords有機物一時貯蔵 / 下水処理 / 活性汚泥 / 省エネルギー / 高速シークエンシング / 微生物群集構造 / 生物学的リン除去 / 栄養塩循環 / 活性汚泥法
Research Abstract

The present study aimed to develop novel sewage treatment technologies with improved energy efficiencies by utilizing microbial ecology. Based on the finding 1) activated sludge can remove organic pollutants with less oxygen consumption under overloaded condition, and 2) temporal carbon storage materials accumulated inside microbial cells are stable during sludge thickening but are readily be degraded to methane in anaerobic digester, a new modified activated sludge was proposed. In addition, a new operational strategy to contribute to peak-cut of electrical power demand was proposed. As the basic aspect on activated sludge microbial ecosystems, the release timing of bacteriophages was studied, an attempt to get a clue on the lysed host species was made, and microbial interactions mediated by chemicals was conducted.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (51 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (15 results) (of which Peer Reviewed: 13 results) Presentation (32 results) (of which Invited: 1 results) Remarks (4 results)

  • [Journal Article] Dynamics of dsDNA viruses hosted by indigenous microorganisms in activated sludge in the treatment of synthetic wastewater2014

    • Author(s)
      Yang H., Satoh H., Mino T.
    • Journal Title

      J. Water Env. Technol.

      Volume: 13(3) Pages: 223-232

    • NAID

      130004438756

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Bacterial Communities in Treated Water and Activated Sludge and Evaluation of an Easy Methodology for Preparing PCR-Compatible DNA Extracts2014

    • Author(s)
      Gunawardana E.G.W., Satoh H., Mino T.
    • Journal Title

      Journal of Water and Environment Technology

      Volume: 12 Issue: 1 Pages: 1-12

    • DOI

      10.2965/jwet.2014.1

    • NAID

      130003391639

    • ISSN
      1348-2165
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Anaerobic Digestion of Polyhydroxybutyrate Accumulated in Excess Activated Sludge2013

    • Author(s)
      Huda S.M.S., Satoh H., Mino T.
    • Journal Title

      Journal of Water and Environment Technology

      Volume: 11 Issue: 5 Pages: 429-438

    • DOI

      10.2965/jwet.2013.429

    • NAID

      130004933692

    • ISSN
      1348-2165
    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Monitoring of ribosomal RNA in the supernatant of activated sludge2013

    • Author(s)
      Yang H., Suda W., Oshima K., Hattori M., Satoh H., Mino T.
    • Journal Title

      土木学会論文集G

      Volume: 69(7)

    • NAID

      130004962542

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The effect of reduction of aeration period on organic pollutants removal in sequencing batch activated sludge reactors2013

    • Author(s)
      Shi W., Satoh H., Mino T.
    • Journal Title

      土木学会論文集G

      Volume: 69

    • NAID

      130004962566

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Microbial Community Composition of Polyhydroxyalkanoate-Accumulating Organisms in Full-Scale Wastewater Treatment Plants Operated in Fully Aerobic Mode2013

    • Author(s)
      M. Oshiki
    • Journal Title

      Microbes and Environments

      Volume: 28 Issue: 1 Pages: 96-104

    • DOI

      10.1264/jsme2.ME12141

    • NAID

      10031138549

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monitoring of ribosomal RNA in the supernatant of activated sludge.2013

    • Author(s)
      Yang H., Suda W., Oshima K., Hattori M., Satoh H., Mino T.
    • Journal Title

      土木学会論文集G(環境)

      Volume: 69(7)

    • NAID

      130004962542

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effect of reduction of aeration period on organic pollutants removal in sequencing batch activated sludge reactors.2013

    • Author(s)
      Shi W., Satoh H., Mino T.
    • Journal Title

      土木学会論文集G(環境)

      Volume: 69

    • NAID

      130004962566

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bacterial Population Dynamics in a Laboratory Activated Sludge Reactor Monitored by Pyrosequencing of 16S rRNA2013

    • Author(s)
      Satoh, H., Oshima, K., Suda, W., Ranasinghe, P., Li, N., Gunawardana, E.G.W., Hattori, M., Mino, T.
    • Journal Title

      Microbes and Environments

      Volume: 28 Issue: 1 Pages: 65-70

    • DOI

      10.1264/jsme2.ME12115

    • NAID

      10031138545

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamics of dewaterability and bacterial populations in activated sludge2012

    • Author(s)
      Li N., Satoh H., Mino T.
    • Journal Title

      Water Sci. Technol.

      Volume: 66 Issue: 8 Pages: 1634-1640

    • DOI

      10.2166/wst.2012.360

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Revealing microbial community structures in large- and small-scale activated sludge systems by barcoded pyrosequencing of 16S rRNA gene.2012

    • Author(s)
      Ranasinghe, P.D., Satoh, H., Oshiki, M., Oshima, K., Suda, W., Hattori, M., Mino, T.
    • Journal Title

      Water Science and Technology

      Volume: 66 Issue: 10 Pages: 2155-2161

    • DOI

      10.2166/wst.2012.428

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 次世代シークエンサーが照らし出す廃水処理微生物生態系2012

    • Author(s)
      佐藤弘泰
    • Journal Title

      日本水環境学会誌

      Volume: 35A Pages: 308-314

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Rapid Quantification of Polyhydroxyalkanoates (PHA) Concentration in Activated Sludge with the Fluorescent dye Nile Blue A2011

    • Author(s)
      Oshiki, M., H.Satoh, T.Mino
    • Journal Title

      Water Science and Technology

      Volume: 64巻 Issue: 3 Pages: 747-753

    • DOI

      10.2166/wst.2011.707

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 活性汚泥の脱水性に影響を与えるバクテリア群の探索2011

    • Author(s)
      李寧、佐藤弘泰、味埜俊
    • Journal Title

      第45回日本水環境学会年会

      Pages: 684-684

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Comparison of Bacterial Communities in Treated Water and in Activated Sludge.2010

    • Author(s)
      Gunawardana, E.G.W., Satoh, H.Mino, T.
    • Journal Title

      環境工学研究論文集

      Volume: 47 Pages: 357-364

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 活性汚泥法における遅滞曝気による電力消費のピークカット2014

    • Author(s)
      石維, 庄司仁, 佐藤弘泰, 味埜俊
    • Organizer
      第48回日本水環境学会年会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Rapid detection of microbial population changes caused by extracts from activated sludge.2014

    • Author(s)
      蘇涛, 佐藤弘泰, 味埜俊
    • Organizer
      第48回日本水環境学会年会
    • Place of Presentation
      東北大学(仙台)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Nature of polyhydroxyalkanoates accumulated in activated sludge as a possible source of bioenergy.2013

    • Author(s)
      Huda S.M.S., Satoh H., Mino T.
    • Organizer
      IWA-ASPIRE 2013 Conference and Exhibition
    • Place of Presentation
      Daejung, Korea
    • Related Report
      2013 Annual Research Report
  • [Presentation] 次世代型シークエンサーを用いた活性汚泥細菌群集構造の通年モニタリング2013

    • Author(s)
      佐藤弘泰、Gunawardana E.G.W.、李寧、味埜俊、須田亙、大島健志郎、服部正平
    • Organizer
      第50回下水道研究発表会講演集
    • Place of Presentation
      東京ビッグサイト(東京)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Rapid detection of activated sludge microbial population changes caused by addition of extract from another activated sludge2013

    • Author(s)
      蘇 涛
    • Organizer
      第47回日本水環境学会年会
    • Place of Presentation
      大阪工業大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微量元素が生物学的リン除去に及ぼす影響の検討2012

    • Author(s)
      佐藤祐樹
    • Organizer
      第46回日本水環境学会年会講演集
    • Place of Presentation
      東洋大学(東京都)
    • Year and Date
      2012-03-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] 余剰汚泥の持つ有機物吸着能力を活かした下水処理エネルギー効率の改善.2012

    • Author(s)
      佐藤弘泰
    • Organizer
      第49回下水道研究発表会講演集
    • Place of Presentation
      神戸国際会議場
    • Related Report
      2012 Annual Research Report
  • [Presentation] Dynamics of bacteriophages in supernatant of wastewater treatment processes.2012

    • Author(s)
      Yang H.
    • Organizer
      14th International Symposium on Microbial Ecology
    • Place of Presentation
      デンマーク、コペンハーゲン市
    • Related Report
      2012 Annual Research Report
  • [Presentation] T-RFLP and Pyrosequencing Approach to Clarify Bacterial Groups that Affects Dewaterability of Activated Sludge2012

    • Author(s)
      Li N.
    • Organizer
      14th International Symposium on Microbial Ecology
    • Place of Presentation
      デンマーク、コペンハーゲン市
    • Related Report
      2012 Annual Research Report
  • [Presentation] 次世代シークエンサーデータ解析支援ツール“OTUMAMi”の開発とそれを利用した活性汚泥細菌群集解析.2012

    • Author(s)
      佐藤弘泰
    • Organizer
      第15回日本水環境学会シンポジウム
    • Place of Presentation
      佐賀大学
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 下水処理水中に出現したバクテリオファージの経時変化2012

    • Author(s)
      佐藤弘泰
    • Organizer
      第4回ファージ研究会
    • Place of Presentation
      群馬大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 微生物群集構造解析を目的とした超音波破砕活性汚泥サンプルのエタノール添加による保存方法の研究2012

    • Author(s)
      Tao Su
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 高速シークエンシング法による活性汚泥微生物群集構造変化の解析2012

    • Author(s)
      佐藤弘泰
    • Organizer
      第28回日本微生物生態学会大会
    • Place of Presentation
      豊橋技術科学大学
    • Related Report
      2012 Annual Research Report
  • [Presentation] 下水処理エネルギー効率改善のための微生物の有機物一時貯蔵能力の活用2011

    • Author(s)
      佐藤弘泰
    • Organizer
      第48回環境工学研究フォーラム
    • Place of Presentation
      大同大学(名古屋市)
    • Year and Date
      2011-11-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] 微量元素が生物学的リン除去プロセスに及ぼす影響2011

    • Author(s)
      佐藤祐樹
    • Organizer
      第48回環境工学研究フォーラム
    • Place of Presentation
      大同大学(名古屋市)
    • Year and Date
      2011-11-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Anaerobic Digestion of Temporal Carbon Storage Materials Accumulated in Activated Sludge2011

    • Author(s)
      S.M.Shamsul Huda
    • Organizer
      IWA-ASPIRE (International Water Association Asia Pacific Region) 2011
    • Place of Presentation
      東京フォーラム(東京)
    • Year and Date
      2011-10-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of Bacterial Communities in Activated Sludge and in Treated Water while Developing an Easy Methodology for Preparation of PCR-Compatible DNA Extract2011

    • Author(s)
      Egodaha Gedara Wasana Gunawardana
    • Organizer
      IWA-ASPIRE (International Water Association Asia Pacific Region) 2011
    • Place of Presentation
      東京フォーラム(東京)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Revealing Microbial Community Structures in Large and Small Scale Activated Sludge Systems by Barcoded Pyrosequencing of 16S rRNA Gene2011

    • Author(s)
      Ranasinghe, P.D.
    • Organizer
      IWA-ASPIRE (International Water Association Asia Pacific Region) 2011
    • Place of Presentation
      東京フォーラム(東京)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dynamics of Bacteriophages in Treated Water from an Activated Sludge Process2011

    • Author(s)
      Yang H.
    • Organizer
      IWA-ASPIRE (International Water Association Asia Pacific Region) 2011
    • Place of Presentation
      東京フォーラム(東京)
    • Year and Date
      2011-10-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dynamics of Dewaterability and Bacterial Populations in Activated Sludge2011

    • Author(s)
      Ning Li
    • Organizer
      IWA-ASPIRE (International Water Association Asia Pacific Region) 2011
    • Place of Presentation
      東京フォーラム(東京)
    • Year and Date
      2011-10-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] 16S barcoded pyrosequencing resolves diversity of Betaproteobacteria in activated sludge2011

    • Author(s)
      Ranasinghe, P.D.
    • Organizer
      IUMS (International Union of Microbial Societies) 2011
    • Place of Presentation
      札幌コンベンションセンター(北海道)
    • Year and Date
      2011-09-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] Effect of activated sludge handling on suspended solids and bacterial populations in settled supernatant2011

    • Author(s)
      Egodaha Gedara Wasana GUNAWARDANA
    • Organizer
      環境バイオテクノロジー学会2011年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2011-06-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Methane production from polyhydroxybutyrate accumulated in activated sludge2011

    • Author(s)
      Huda, S.M.S
    • Organizer
      環境バイオテクノロジー学会2011年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2011-06-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Developing a computational tool to illustrate microbial diversity in activated sludge2011

    • Author(s)
      Ranasinghe, P.D.
    • Organizer
      環境バイオテクノロジー学会2011年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2011-06-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Time-shifting of power consumption in wastewater treatment using temporal carbon storage capability of activated sludge microorganisms2011

    • Author(s)
      Shi, W.
    • Organizer
      環境バイオテクノロジー学会2011年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2011-06-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 下水道の復旧復興における微生物の有機物一時貯蔵能力の活用の提案2011

    • Author(s)
      佐藤弘泰
    • Organizer
      環境バイオテクノロジー学会2011年度大会
    • Place of Presentation
      東京大学(東京都)
    • Year and Date
      2011-06-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] 遠心分離と精密天秤を組み合わせた汚泥の脱水性評価手法の開発2010

    • Author(s)
      李寧、佐藤弘泰、味埜俊
    • Organizer
      第47回下水道研究発表会
    • Place of Presentation
      名古屋市
    • Related Report
      2010 Annual Research Report
  • [Presentation] Degradation of excess sludge accumulating polyhydroxybutyrate produced from energy saving activated sludge process under anaerobic digestion.2010

    • Author(s)
      Huda, S.M.S., Satoh, H., Mino, T.
    • Organizer
      The 8th International Symposium on Southeast Asian Water Environment
    • Place of Presentation
      プーケット(タイ)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Pyro-Sequencing-aided Identification of Microorganisms that were Affected by Activated Sludge Extract.2010

    • Author(s)
      Ranasinghe, P.D., Satoh, H., Oshima, K., Hattori, M., Mino, T.
    • Organizer
      第47回環境工学研究フォーラム
    • Place of Presentation
      高知市
    • Related Report
      2010 Annual Research Report
  • [Presentation] Dynamics of Dewaterability of Activated Sludge Elucidated by a Novel Dewaterability Testing Method.2010

    • Author(s)
      Li, N., Satoh, H., andMino, T.
    • Organizer
      3rd IWA Asian Pacific Young Water Professionals Conference
    • Place of Presentation
      シンガポール(シンガポール)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Relationship between bacterial population in activated sludge and phylotype of free DNA in treated water2010

    • Author(s)
      Gunawardana, E.G.W., Satoh, H.Mino, T.
    • Organizer
      第26回日本微生物生態学会年会
    • Place of Presentation
      つくば市
    • Related Report
      2010 Annual Research Report
  • [Presentation] Comparative Study of Bacterial Communities in wastewater Treatment Plants byPyrosequencing of Partial 16S rRNA Gene.2010

    • Author(s)
      Ranasinghe, P.D., Satoh, H., Oshima, K., Hattori, M., Mino, T.
    • Organizer
      第26回日本微生物生態学会年会
    • Place of Presentation
      つくば市
    • Related Report
      2010 Annual Research Report
  • [Remarks] 東京大学大学院新領域創成科学研究科社会文化環境学専攻 味埜佐藤研究室ホームペ-ジ

    • URL

      http://www.mwm.k.u-tokyo.ac.jp:8080/Plone/outcome

    • Related Report
      2013 Final Research Report
  • [Remarks] 研究の成果-東京大学味埜佐藤研究室

    • URL

      http://www.mwm.k.u-tokyo.ac.jp:8080/Plone/outcome

    • Related Report
      2013 Annual Research Report
  • [Remarks] 東京大学大学院新領域創成科学研究科社会文化環境学専攻 味埜佐藤研究室ホームページ

    • URL

      http://www.mwm.k.u-tokyo.ac.jp:8080/Plone

    • Related Report
      2012 Annual Research Report
  • [Remarks]

    • URL

      http://www.mwm.k.u-tokyo.ac.jp:8080/Plone

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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