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Establishment of the methodology for design and growth of implantable pancreatic beta cell or liver cell tissues in vitro

Research Project

Project/Area Number 22246101
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionThe University of Tokyo

Principal Investigator

SAKAI Yasuyuki  東京大学, 生産技術研究所, 教授 (00235128)

Co-Investigator(Kenkyū-buntansha) SHIRAKASHI Ryo  東京大学, 生産技術研究所, 教授 (80292754)
ITOH Taichi  東京大学, 大学院医学系研究科, 准教授 (50447421)
新野 俊樹  東京大学, 生産技術研究所, 准教授 (70291929)
Co-Investigator(Renkei-kenkyūsha) NIINO Toshiki  東京大学, 生産技術研究所, 教授 (70291929)
Research Collaborator YUAN Pang  
YAO-TONG Lai  
STEPHANIE Liana Utami Sutoko  
Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥46,930,000 (Direct Cost: ¥36,100,000、Indirect Cost: ¥10,830,000)
Fiscal Year 2014: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000)
Fiscal Year 2013: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2011: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2010: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Keywords医用化学工学 / マルチスケール血管網配備 / 膵島 / 肝 / 三次元造形 / 人工赤血球 / 肝組織 / 流路ネットワーク / 組織ユニット / 移植 / 再生医療 / 組織工学 / 細胞 / 生物・生体プロセス / 再生医学 / 組織 / 移植・再生医療 / 細胞・組織 / 生物・生体工学
Outline of Final Research Achievements

Toward engineering implantable pancreatic beta cell or liver cell tissues, an integrative methodologies that fully utilizes the advantages of both tissue element assembly in a micro-cale and large 3D scaffold having a flow channel network in a macro-scale. Model scaffolds was fabricated by the 3D selective laser sintering (SLS) process. Good results of the perfusion culture of liver cell aggregates proved the high feasibility of the methodology. In addition, we succeeded in the preparation of hemoglobin-based artificial oxygen carrier showing lower cytotoxicity.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (37 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (14 results) (of which Peer Reviewed: 11 results,  Acknowledgement Compliant: 1 results) Presentation (19 results) (of which Invited: 6 results) Book (2 results) Remarks (2 results)

  • [Journal Article] Preparation of uniform-sized hemoglobin-albumin microspheres as oxygen carriers by Shirasu porous glass membrane emulsification technique2015

    • Author(s)
      Yao-Tong Lai, Mayu Sato, Seiichi Ohta, Kazuki Akamatsu, Shin-ichi Nakao, Yasuyuki Sakai, Taichi Ito
    • Journal Title

      Colloids and Surfaces B: Biointerfaces

      Volume: 127 Pages: 1-7

    • DOI

      10.1016/j.colsurfb.2015.01.018

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Combination of microwell structures and direct oxygenation enables efficient and size-regulated aggregate formation of an insulin-secreting pancreatic beta-cell line2014

    • Author(s)
      M. Shinohara, H. Kimura, K. Montagne, K. Komori, T. Fujii, Y. Sakai
    • Journal Title

      Biotechnol. Prog.

      Volume: 30 Issue: 1 Pages: 178-187

    • DOI

      10.1002/btpr.1837

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] マクロ三次元流路ネットワークと小チャンバーからなる組織再構築用担体2014

    • Author(s)
      酒井康行、厖媛、堀本洋平、安斎正博,新野俊樹
    • Journal Title

      バイオインダストリー

      Volume: 31 Pages: 45-50

    • Related Report
      2013 Annual Research Report
  • [Journal Article] 酸素供給に基づいた肝組織の設計と構築2014

    • Author(s)
      酒井康行,厖媛,堀本洋平,安斎正博,新野俊樹
    • Journal Title

      伝熱学会誌

      Volume: 53 Pages: 10-15

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Preparation of Uniform-Sized Poly[methacryloxypropyl Tris(trimethylsiloxy)silane] Microspheres via Shirasu Porous Glass Membrane Emulsification Technique2013

    • Author(s)
      Yao-Tong Lai, Seiichi Ohta, Kazuki Akamatsu, Shin-ichi Nakao, Yasuyuki Sakai, Taichi Ito
    • Journal Title

      JOURNAL OF CHEMICAL ENGINEERING OF JAPAN

      Volume: 46 Issue: 11 Pages: 777-784

    • DOI

      10.1252/jcej.13we100

    • NAID

      130003381990

    • ISSN
      0021-9592, 1881-1299
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation and harvesting of thick pancreatic β-cell sheets on a highly O2-permeable plate modified with poly(N-isopropylacrylamide)2013

    • Author(s)
      Kikuo Komori,* Mari Udagawa, Marie Shinohara, Kevin Montagne, Tatsuro Tsuru and Yasuyuki Sakai
    • Journal Title

      Biomaterials Science

      Volume: 1 Issue: 5 Pages: 510-518

    • DOI

      10.1039/c3bm00123g

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid aggregation of heterogeneous cells and multiple-sized microspheres in methylcellulose medium2012

    • Author(s)
      N. Kojima, S. Takeuchi and Y. Sakai
    • Journal Title

      Biomaterials

      Volume: 33 Issue: 18 Pages: 4508-4514

    • DOI

      10.1016/j.biomaterials.2012.02.065

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Liver tissue engineering based on aggregate assembly: efficient formation of endothelialized rat hepatocyte aggregates and their immobilization with biodegradable fibers2012

    • Author(s)
      Y. Pang, K. Montagne, M. Shinohara, K. Komori and Y. Sakai
    • Journal Title

      Biofabrication

      Volume: 4 Issue: 4 Pages: 45004-45004

    • DOI

      10.1088/1758-5082/4/4/045004

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Laser sintering fabrication of three-dimensional tissue engineering scaffolds with a flow channel network2011

    • Author(s)
      T.Niino, D.Hamajima, K.Montagne, S.Oizumi, H.Naruke, H.Huang, Y.Sakai
    • Journal Title

      Biofabrication

      Volume: 3 Issue: 3 Pages: 34-104

    • DOI

      10.1088/1758-5082/3/3/034104

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Avidin-biotin-based approach to forming heterotypic cell clusters and cell sheets on a gas-permeable membrane2011

    • Author(s)
      M.Hamon, K.Montagne, N.Kojima, I.Ozawa, R.Ishii, S.Yamaguchi, T.Nagamune, T.Ushida, Y.Sakai
    • Journal Title

      Biofabrication

      Volume: 3 Issue: 3 Pages: 34-111

    • DOI

      10.1088/1758-5082/3/3/034111

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Use of liposome encapsulated hemoglobin as an oxygen carrier for fetal and adult rat liver cell culture2011

    • Author(s)
      K.Montagne, H.Huang, K.Ohara, K.Matsumoto, A.Mizuno, K.Ohta, Y.Sakai
    • Journal Title

      J.Biosci.Bioeng

      Volume: 112 Pages: 485-490

    • NAID

      110008761513

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 再生医療への化学工学の寄与可能性-組織設計・構築と育成-2010

    • Author(s)
      酒井康行, 藤井輝夫, 新野俊樹
    • Journal Title

      再生医療

      Volume: 9 Pages: 361-366

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Spontaneous formation of stably-attached and 3D-organized hepatocyte aggregates on oxygen-permeable polydimethylsiloxane membranes having 3D microstructures2010

    • Author(s)
      F.Evenou, T.Fujii, Y.Sakai
    • Journal Title

      Biomedical Microdevices

      Volume: 12 Pages: 465-475

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous formation of highly functional three-dimensional multilayer from human hepatoma Hep G2 cells cultured on an oxygen-permeable polydimethylsiloxane membrane2010

    • Author(s)
      F.Evenou, T.Fujii, Y.Sakai
    • Journal Title

      Tissue Engineering Part C : Methods

      Volume: 16 Pages: 311-318

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] SPG膜乳化型酸素運搬体による肝細胞への酸素供給の検討2015

    • Author(s)
      Yao-Tong Lai・太田誠一・赤松憲樹・中尾真一・酒井康行・伊藤大知
    • Organizer
      第14回 日本再生医療学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2015-03-29 – 2015-03-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヘモグロビン/アルブミンマイクロスフィア型人工酸素運搬体の細胞取り込みと生体適合性に与えるサイズの影響2014

    • Author(s)
      Yao-Tong Lai・太田誠一・赤松憲樹・中尾真一・酒井康行・伊藤大知
    • Organizer
      第21回血液代替物学会
    • Place of Presentation
      東京
    • Year and Date
      2014-12-08 – 2014-12-09
    • Related Report
      2014 Annual Research Report
  • [Presentation] 細胞・組織培養と生理学的酸素供給2014

    • Author(s)
      酒井康行
    • Organizer
      第12回ガンとハイポキシア研究会
    • Place of Presentation
      佐賀
    • Year and Date
      2014-11-21
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Engineering of Implantable Liver Tissue Equivalent based on Three-dimensional Scaffold fabrication and Cellular Aggregate Assembly2014

    • Author(s)
      Yuan PANG, Yohei HORIMOTO, Toshiki NIINO, Yasuyuki SAKAI
    • Organizer
      The 4th International Conference on Additive Manufacturing and Bio-Manufacturing
    • Place of Presentation
      北京
    • Year and Date
      2014-11-12 – 2014-11-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] Design, fabrication and performance evaluation in packed-bed perfusion culture of liver cells2014

    • Author(s)
      S. Sutoko, Y. Pang, Y. Horimoto, M. Anzai, T. Niino, Y. Sakai
    • Organizer
      International Conference on Biofabrication 2014
    • Place of Presentation
      Pohang, Korea
    • Year and Date
      2014-09-28 – 2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Design and fabrication of scalable three‐dimensional scaffold for in vitro liver tissue engineering2014

    • Author(s)
      Yuan PANG, Yohei HORIMOTO, Stephanie Sutoko, Toshiki NIINO, Yasuyuki SAKAI
    • Organizer
      International Conference on Biofabrication 2014
    • Place of Presentation
      Pohang, Korea
    • Year and Date
      2014-09-28 – 2014-10-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] Size control of hemoglobin and albumin-based oxygen carriers by SPG membrane emulsification2014

    • Author(s)
      Lai Yao-Tong・太田誠一・赤松憲樹・中尾真一・酒井康行・伊藤大知
    • Organizer
      化学工学会 第46回 秋期大会
    • Place of Presentation
      九州大学
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] ボトムアップとトップダウンを融合する大型組織構築の方法論2014

    • Author(s)
      酒井康行,厖媛,Stephanie L. U. SUTOKO,堀本洋平,安斎正博,新野俊樹
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] SPG膜乳化法を用いたヘモグロビン/アルブミン架橋型人工酸素運搬体の開発2013

    • Author(s)
      Yao-Tong Lai・太田誠一・佐藤真優・赤松憲樹・中尾真一・酒井康行・伊藤大知
    • Organizer
      第20回血液代替物学会
    • Place of Presentation
      奈良市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Preparation of a novel hemoglobin-based oxygen carrier by the Shirasu porous glass membrane emulsification technique2013

    • Author(s)
      Yao-Tong Lai, SeiichiOhta, Kazuki A kamatsu, Shinichi Nakao, Yasuyuki Sakai, Taichi Ito
    • Organizer
      第51回日本人工臓器学会大会/第5回 国際人工臓器学術大会
    • Place of Presentation
      横浜市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Microfabrication and chemical Engineering in organization of liver tissues for various applications2013

    • Author(s)
      Yasuyuki Sakai
    • Organizer
      Grand Challenge for Integration of Stem Cell, Nanomaterials and Biomanufacturing - Looking for Synergy” (SCNB2013)
    • Place of Presentation
      上海
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 酸素透過性材料を用いた厚いシート状肝・膵島組織の作成2012

    • Author(s)
      酒井康行、Fanny Evenou、Morgan Hamon、篠原満利恵、小森喜久夫、藤井輝夫
    • Organizer
      日本再生医療学会第11回総会
    • Place of Presentation
      パシフィコ横浜
    • Related Report
      2012 Annual Research Report
  • [Presentation] Micro-technologies and chemical engineering for organization of liver tissue2012

    • Author(s)
      Y. Sakai
    • Organizer
      6th International Conference on Bioengineering and Nanotechnology
    • Place of Presentation
      Berkeley, USA
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Self-secreted factors, oxygen and 3D culture in stem/progenitor cell-based tissue engineering ― mES/iPS, liver and pancreatic b-cells ―2012

    • Author(s)
      Y. Sakai
    • Organizer
      1st Joint Symposium 2012 on Bridging Cancer Nonotechnology, The University of Texas MD Anderson Cancer Center & University of Tokyo
    • Place of Presentation
      Houston, USA
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] New 3D scaffold consisting of assembled subunits as culture chambers and an interconnected flow-channel network for liver tissue engineering2012

    • Author(s)
      Yuan PANG, Yohei HORIMOTO, kentaro HARA, Yutaro ITAGAKI, Toshiki NIINO, Yasuyuki SAKAI
    • Organizer
      International Conference on Biofabrication 2012
    • Place of Presentation
      Manchester, UK
    • Related Report
      2012 Annual Research Report
  • [Presentation] Chemical engineering-based multi-scale optimization of 3D cellular organization and oxygen supply2011

    • Author(s)
      Y.Sakai
    • Organizer
      2011 MRS Fall Meeting & Exhibition
    • Place of Presentation
      ボストン,米国(招待講演)
    • Year and Date
      2011-11-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Scaffolds having flow channels, oxygen carriers and oxygen-permeable membranes2011

    • Author(s)
      Yasuyuki SAKAI, Teruo FUJII, Toshiki NIINO
    • Organizer
      HAAISHAPD 2011 Annual Scientific Meeting
    • Place of Presentation
      シドニー,オーストラリア(招待講演)
    • Year and Date
      2011-11-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] Importance of oxygen supply in liver tissue engineering : Scaffolds having flow channels, oxygen carriers and oxygen-permeable membranes2010

    • Author(s)
      Yasuyuki Sakai, Toshiki Niino
    • Organizer
      BIT Life Sciences' 3rd Annual Congress of Regenerative Medicine & Stem Cell-2010
    • Place of Presentation
      中国,上海
    • Year and Date
      2010-12-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 生体に学ぶ:人工赤血球とハイドロゲルニッチ設計の試み

    • Author(s)
      伊藤大知
    • Organizer
      オレオナノサイエンスシンポジウム2013-生物に学ぶ生体界面科学-
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Book] "Engineering of implantable liver tissues", in "Liver Stem Cells"(etd. by T. Ochiya)2011

    • Author(s)
      Y.Sakai, M.Nishikawa, F.Evenou, M.Hamon, H.Huang, K.
    • Publisher
      Hamana Press
    • Related Report
      2011 Annual Research Report
  • [Book] 第18章,血流導入型体内埋め込み組織の構築,「細胞治療・再生医療のための培養システム」(紀ノ岡正博,酒井康行監修)2011

    • Author(s)
      酒井康行, 新野俊樹(分担執筆)
    • Publisher
      シーエムシー出版
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://envchem.iis.u-tokyo.ac.jp/sakai/index.php

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://envchem.iis.u-tokyo.ac.jp/sakai/index.php?lang=ja&page=research&category=5

    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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