| Budget Amount *help |
¥42,770,000 (Direct Cost: ¥32,900,000、Indirect Cost: ¥9,870,000)
Fiscal Year 2014: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2012: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2011: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2010: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Outline of Final Research Achievements |
Homologous DNA recombination (HR) is regulated by double-strand DNA-break (DSB) formation and recombination-pathway choice among two types of HR (non-crossing-over and crossing-over) and non-homologous end-joining (NHEJ). (1) Using new bio-assays, we identified rice and Arabidopsis SPO11s (meiotic recombination-initiating proteins) and their intrinsic DSB-forming activity for the first time. (2) We identified using a newly devised genetic assay, DSB-repair pathway-choice functions of Srs2 helicase, which forms an active complex with Rad51 (a eukaryotic RecA-family recombinase). (3) We unexpectedly found that Rad51 functions in end-joining fidelity of the canonical NHEJ. (4) We revealed by structural and biochemical studies, roles of an acidic residue on a flexible loop of RecA in selecting ssDNA in its various in vitro functions, and in activation and inactivation of its recombinase activity. These results suggest new roles of RecA/Rad51 in all the three major DSB-repair pathways.
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