Evolutional strategy for the establishment of angiosperm-type chloroplast NDH

• Project/Area Number: 22247005
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyoto University
• Principal Investigator: SHIKANAI TOSHIHARU 京都大学, 理学(系)研究科(研究院), 教授 (70273852)
• Co-Investigator(Renkei-kenkyūsha): TSUGEKI Ryuuji 京都大学, 大学院理学研究科, 助教 (50303805) ; NISHIMURA Yoshiki 京都大学, 大学院理学研究科, 助教 (70444099)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥43,940,000 (Direct Cost: ¥33,800,000、Indirect Cost: ¥10,140,000); Fiscal Year 2012: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2011: ¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000); Fiscal Year 2010: ¥17,160,000 (Direct Cost: ¥13,200,000、Indirect Cost: ¥3,960,000)
• Keywords: 葉緑体 / NDH / サイクリック電子伝達 / 光合成 / アセンブリ / 進化 / シロイヌナズナ / ゼニゴケ / サイクイック電子伝達 / レドックス調節 / 電子伝達 / 分子シャペロン / アッセンブリー / コケ植物

Research Abstract

We discovered that a novel protein, NdhS is necessary for the high-affinity binding of chloroplast NDH with ferredoxin. Chloroplast NDH is not NAD(P)H dehydrogenase but antimycin A-resistant ferredoxin-dependent plastoquinone reductase (FQR). We also clarified the assembly process of subcomplex A of chloroplast NDH in Arabidopsis with the discovery of several assembly factors specifically required for this process. We further discovered that in Marchantia chloroplast NDH does not form the supercomplex with photosystem I and is involved in the redox homeostasis in chloroplasts at low light intensity.

Research Products

• [Journal Article] Central role of cyclic electron transport around photosystem I in the regulation of photosynthesis (2014)
  • Author(s): Shikanai T
  • Journal Title: Curr. Opin. Biotech. Volume: 26 Pages: 25-30
  • DOI: 10.1016/j.copbio.2013.08.012
• [Journal Article] In planta mutagenesis of Src homology 3 domain-like fold of NdhS, a ferredoxin-binding subunit of the chloroplast NADH dehydrogenase-like complex in Arabidopsis. A conserved Arg-193 plays a critical role in ferredoxin binding (2013)
  • Author(s): Yamamoto H. and Shikanai T
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 51 Pages: 36328-36337
  • DOI: 10.1074/jbc.m113.511584
  • Peer Reviewed
• [Journal Article] Antimycin A-like molecules inhibit cyclic electron transport around photosystem I in ruptured chloroplasts (2013)
  • Author(s): Taira Y., Okegawa Y., Sugimoto K., Abe M., Miyoshi H. and Shikanai T
  • Journal Title: FEBS Open Bio. Volume: 3 Issue: 1 Pages: 406-410
  • DOI: 10.1016/j.fob.2013.09.007
  • Peer Reviewed / Open Access
• [Journal Article] A single amino acid alteration in PGR5 confers resistance to antimycin A in cyclic electron transport around PSI (2013)
  • Author(s): Sugimoto K., Okegawa Y., Tohri A., Long T.A., Sarah F.S., Hisabori T. and Shikanai T
  • Journal Title: Plant Cell Physiol. Volume: 54 Issue: 9 Pages: 1525-1534
  • DOI: 10.1093/pcp/pct098
  • Peer Reviewed
• [Journal Article] Mutagenesis of Individual Pentatricopeptide Repeat Motifs Affects RNA Binding Activity and Reveals Functional Partitioning of Arabidopsis PROTONGRADIENT REGULATION3 (2013)
  • Author(s): Fujii S, Sato N, Shikanai T
  • Journal Title: Plant Cell Volume: 25 Issue: 8 Pages: 1-11
  • DOI: 10.1105/tpc.113.112193
  • Peer Reviewed
• [Journal Article] PGR 5-dependent cyclic electron transport around PSI contributes to the redox homeostasis in chloroplasts rather than CO_2 fixation and biomass production in rice (2012)
  • Author(s): Nishikawa Y, Yamamoto H, Okegawa Y, Wada S, Sato N, Taira Y, Sugimoto K, Makino A and Shikanai T
  • Journal Title: Plant Cell Physiol Volume: 53 Issue: 12 Pages: 2117-2126
  • DOI: 10.1093/pcp/pcs153
  • NAID: 10031145641
  • Peer Reviewed
• [Journal Article] Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha (2012)
  • Author(s): Ueda M., Kuniyoshi T., Yamamoto Y., Sugimoto K., Ishizaki K., Kohchi T., Nishimura Y. and Shikanai T
  • Journal Title: Plant J. Volume: 72 Issue: 4 Pages: 683-693
  • DOI: 10.1111/j.1365-313x.2012.05115.x
  • Peer Reviewed
• [Journal Article] Multistep assembly of chloroplast NADH dehydrogenase-like subcomplex A requires several nucleus-encoded proteins including CRR41 and CRR42, in Arabidopsis (2012)
  • Author(s): Peng L, Fukao Y, Fujiwara M, Shikanai T
  • Journal Title: Plant Cell Volume: 24 Issue: 1 Pages: 202-214
  • DOI: 10.1105/tpc.111.090597
  • Peer Reviewed
• [Journal Article] Structure of the chloroplast NADH dehydrogenase-like complex : nomenclature for nuclear-encoded subunits (2011)
  • Author(s): Ifuku K, Endo T, Shikanai T, Aro E-M
  • Journal Title: Plant Cell Physiol Volume: 52 Issue: 9 Pages: 1560-1568
  • DOI: 10.1093/pcp/pcr098
  • NAID: 10029714253
  • Peer Reviewed
• [Journal Article] An Src homology 3 domain-like fold protein forms a ferredoxin-binding site for the chloroplast NADH dehydrogenase-like complex in Arabidopsis (2011)
  • Author(s): Yamamoto H., Peng L., Fukao Y. and Shikanai T
  • Journal Title: Plant Cell Volume: 23 Issue: 4 Pages: 1480-1493
  • DOI: 10.1105/tpc.110.080291
• [Journal Article] A chaperonin subunit with unique structures is essential for folding of a specific substrate (2011)
  • Author(s): Peng L., Fukao Y., Myouga F., Motohashi R., Shinozaki K. and Shikanai T
  • Journal Title: PLoS Biol. Volume: 9 Issue: 4 Pages: e1001040-e1001040
  • DOI: 10.1371/journal.pbio.1001040
• [Journal Article] Supercomplex formation with photosystem I is required for the stabilization of the chloroplast NADH dehydrogenase-like complex in Arabidopsis (2011)
  • Author(s): Peng L. and Shikanai
  • Journal Title: Plant Physiol. Volume: 155 Issue: 4 Pages: 1629-1639
  • DOI: 10.1104/pp.110.171264
• [Journal Article] Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase complex (2011)
  • Author(s): Peng L., Yamamoto H. and Shikanai T
  • Journal Title: Biochim. Biophys. Acta Volume: 1807 Issue: 8 Pages: 945-953
  • DOI: 10.1016/j.bbabio.2010.10.015
• [Journal Article] PROTON GRADIENT REGULATION 3 recognizes multiple targets with limited similarity and mediates translation and RNA stabilization in plastids (2011)
  • Author(s): Cai W, Okuda K, Peng L, Shikanai T
  • Journal Title: Plant J Volume: 67 Issue: 2 Pages: 318-327
  • DOI: 10.1111/j.1365-313x.2011.04593.x
  • Peer Reviewed
• [Journal Article] Supercomplex formation with photosystem I is required for the stabilization of the chloroplast NADH dehydrogenase-like complex in Arabidopsis. (2011)
  • Author(s): Peng L, Shikanai T
  • Journal Title: Plant Physiology Volume: 155 Pages: 1629-1639
  • Peer Reviewed
• [Journal Article] A chaperonin subunit with unique structures is essential for folding of a specificsubstrate. (2011)
  • Author(s): Peng L, Fukao Y, Myouga F, Motohashi R, Shinozaki K, Shikanai T
  • Journal Title: PLoS Biology Volume: 9
  • Peer Reviewed
• [Journal Article] A Src homology 3 domain-like fold protein forms a ferredoxin-binding site for the chloroplast NADH dehydrogenase-like complex in Arabidopsis. (2011)
  • Author(s): Yamamota H, Peng L, Fukao Y, Shikanai T.
  • Journal Title: The Plant Cell Volume: 22(印刷中)
  • Peer Reviewed
• [Journal Article] Structure and biogenesis of the chloroplast NAD(P)H dehydrogenase complex (2011)
  • Author(s): Peng L, Yamamoto H, Shikanai T
  • Journal Title: Biochimica Biophysica Acta Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Chloroplast stromal proteins, CRR6 and CRR7, are required for assembly of the NAD(P)H dehydrogenase subcomplex A in Arabidopsis. (2010)
  • Author(s): Peng L, Cai W, Shikanai T
  • Journal Title: The Plant Journal Volume: 63 Pages: 203-211
  • Peer Reviewed
• [Journal Article] Physiological links among alternative electron transport pathways reducing and oxidizing plastoquinone in Arabidopsis. (2010)
  • Author(s): Okegawa Y, Kobayashi Y, Shikanai T
  • Journal Title: The Plant Journal Volume: 63 Pages: 458-468
  • Peer Reviewed
• [Journal Article] De novo biosynthesis of fatty acids plays critical roles in the response of the photosynthetic machinery to low temperature in Arabidopsis. (2010)
  • Author(s): Takami T, Sibata M, Kobayashi Y, Shikanai T
  • Journal Title: Plant Cell Physiology Volume: 51 Pages: 1265-1275
  • NAID: 10027351358
  • Peer Reviewed
• [Presentation] Machinery and function of PSI cyclic electron transport (2014)
  • Author(s): Shikanai T
  • Organizer: 2nd Kyoto-Bristol Symposium
  • Place of Presentation: Kyoto
  • Year and Date: 2014-01-10
• [Presentation] Regulation of photosynthetic electron transport (2013)
  • Author(s): Shikanai T
  • Organizer: NC-CARP•CREST/さきがけ国際シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2013-11-08
• [Presentation] Regulation of photosynthesis by PSI cyclic electron transport (2013)
  • Author(s): Shikanai T
  • Organizer: PhD Summer School : New Frontiers in Photosynthesis
  • Place of Presentation: San Michele all'Adige, Italy
  • Year and Date: 2013-07-30
• [Presentation] Regulation of photosynthesis by PSI cyclic electron transport (2013)
  • Author(s): Shikanai T
  • Organizer: 24th International Conference on Arabidopsis Research
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2013-06-25
• [Presentation] Regulation of photosynthesis by PSI cyclic electron transport (2013)
  • Author(s): Shikanai T
  • Organizer: 3^<rd> International Symposium on Chloroplast Genomics and Genetic Engineering
  • Place of Presentation: New Brunswick, USA
  • Year and Date: 2013-05-11
• [Presentation] Cyclic electron flow : new structural components and regulation (2012)
  • Author(s): Shikanai T
  • Organizer: IPMB 2012
  • Place of Presentation: Jeju, Korea
  • Year and Date: 2012-10-23
• [Presentation] Assembly of the chloroplast NDH complex (2012)
  • Author(s): Shikanai T
  • Organizer: The 11th Nordic Photosynthesis Congress
  • Place of Presentation: Naantali, Finland
  • Year and Date: 2012-09-12
• [Presentation] Chloroplast NADH dehydrogenase- like complex : function and assembly (2012)
  • Author(s): Shikanai T
  • Organizer: Gordon Research Conference, Mitochondria & Chloroplasts
  • Place of Presentation: Smithfield, USA
  • Year and Date: 2012-08-01
• [Presentation] Structure and assembly of the chloroplast NDH complex (2012)
  • Author(s): Shikanai T
  • Organizer: NU Plant Science Symposium 2012
  • Place of Presentation: Jinju, Korea
  • Year and Date: 2012-05-11
• [Presentation] Regulation of photosynthetic electron transport : Structure and assembly of thechloroplast NDH complex (2011)
  • Author(s): Shikanai T, Peng L, Yamamoto H
  • Organizer: Japanese-Finish seminar 2011
  • Place of Presentation: 岡山ロイヤルホテル(岡山市)
  • Year and Date: 2011-03-02
• [Presentation] Machinery and assembly of the chloroplast NDH complex. (2011)
  • Author(s): Shikanai T, Peng L, Yamamoto H
  • Organizer: 2011 Switzerland-Japan Workshop
  • Place of Presentation: Ollon, Swiss
  • Year and Date: 2011-01-03
• [Presentation] Machinery and assembly of PSI cyclic electron transport (2011)
  • Author(s): Shikanai T
  • Organizer: Gordon Research Conference, Photosynthesis
  • Place of Presentation: Davidson, USA
• [Presentation] Machinery and assembly of the chloroplast NDH complex (2011)
  • Author(s): Shikanai T
  • Organizer: witzerland-Japan Workshop
  • Place of Presentation: Ollon, Swiss
• [Presentation] Regulation of photosynthetic electron transport by PSI cyclic electron transport (2011)
  • Author(s): Shikanai T
  • Organizer: The 15^<th> International Congress of Photosynthesis
  • Place of Presentation: Beijing, China
• [Presentation] Machinery and assembly of PSI cyclic electron transport (2011)
  • Author(s): Shikanai T
  • Organizer: Gordon Reserch Conference Photosynthesis
  • Place of Presentation: Davidson, NC, USA(招待講演)
• [Presentation] Regulation of photosynthetic electron transport by PSI cyclic electron transport. (2010)
  • Author(s): Shikanai T
  • Organizer: The 15^<th> International Congress of Photosynthesis
  • Place of Presentation: 北京(中国)
  • Year and Date: 2010-08-26
• [Presentation] Structure and function of machinery of photosystem I cyclic electron transport (2010)
  • Author(s): Shikanai T
  • Organizer: APRU Research Symposium
  • Place of Presentation: Kyoto
• [Presentation] Regulation of photosynthesis by PSI cyclic electron transport
  • Author(s): Shikanai T
  • Organizer: 3rd International Symposium on Chloroplast Genomics and Genetic Engineering
  • Place of Presentation: New Brunswick, USA
  • Invited
• [Presentation] Regulation of photosynthesis by PSI cyclic electron transport
  • Author(s): Shikanai T
  • Organizer: PhD Summer School: New Frontiers in Photosynthesis
  • Place of Presentation: San Michele all’Adige, Italy
  • Invited
• [Book] Regulation of photosynthetic electron transport. In The Chloroplast : Basics and Application (2010)
  • Author(s): Shikanai T
  • Publisher: Springer