| Budget Amount *help |
¥43,550,000 (Direct Cost: ¥33,500,000、Indirect Cost: ¥10,050,000)
Fiscal Year 2013: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2011: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Fiscal Year 2010: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
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| Research Abstract |
We investigated the regulatory mechanisms that underlie the derivation of the neural plates (neural precursors) from the pan-somatic tissue precursor epiblast. We took advantage of mutant mouse embryos and epiblast stem cells under culture conditions.
The anterior neural plate, as marked by Sox2 N2 enhancer activity, was derived directly from the epiblast through the cooperative action of transcription factors that promote neural development but repress the development of all non-neural lineages. By contrast, the posterior neural plate, as marked by Sox2 N1 enhancer activity, developed through neuro-mesodermal bipotential precursors named "axial stem cells". Tbx6-dependent repression of the N1 enhancer was required for mesodermal development. These new findings revise the classical view of somatic cell lineage separation, where germ layer separation was assumed to be the essential first step.
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