| Budget Amount *help |
¥45,890,000 (Direct Cost: ¥35,300,000、Indirect Cost: ¥10,590,000)
Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2013: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2010: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
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| Outline of Final Research Achievements |
We identified several host proteins associated with viral RNA replication complexes of Red clover necrotic mosaic virus (RCNMV) and determined their roles in viral RNA replication. RCNMV encodes the replication proteins, p27 and p88. We found that p27 interacts with Hsp70 and Hsp90 and regulate the assembly of the RCNMV replicase complex on the ER membrane. p27 recruits small GTPases, such as ADP ribosylation factor 1 (Arf1) and Sar1, which regulate the biogenesis of COPI and COPII vesicles, respectively, to the ER. These results suggest that the replication of RCNMV depends on the host membrane trafficking machinery and that RCNMV rewires the cellular trafficking pathways to build a viral replication factory. We also found that p88 interacts with phosphatidic acid (PA)-producing enzyme phospholipase D (PLD) and that PLD is essential for viral RNA replication. Together, our findings suggest that RCNMV hijacks many host proteins to achieve successful RNA replication in host cells.
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