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Molecular Understanding of STOP1-regulating system in plant stress tolerance

Research Project

Project/Area Number 22248006
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionGifu University

Principal Investigator

KOYAMA HIROYUKI  岐阜大学, 応用生物科学部, 教授 (90234921)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Yoshiharu  岐阜大学, 応用生物科学部, 教授 (50301784)
IUCHI Satoshi  独立行政法人理化学研究所, 実験植物開発室, 研究員 (90312256)
SUZUKI Yuji  東北大学, 農学研究科, 助教 (80374974)
SAKURAI Nozomu  (財)かずさDNA研究所, 植物ゲノムバイテク室, 研究員 (30392286)
Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000)
Fiscal Year 2013: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2012: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2011: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2010: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
KeywordsSTOP1 / 植物免疫 / 酸性土壌耐性 / アルミニウム / 転写誘導 / 分子育種 / 酸性土壌 / 進化 / 酸耐性 / アルミニウム耐性 / バイオインフォマティクス
Research Abstract

STOP1, a zinc finger protein, regulates transcription of Al and proton tolerance genes in Arabidopsis. We characcterized function of orthologous genes in various plant species, including woody plants, grass plants, and bryophyte. All plants conserved functional protein of STOP1-orthologues, which regulate expression of various acid and Alresistant genes. It indicated STOP1-regulating system is ubiquitus among wide range of land plant speceis.
Expression of ALMT1, encoding malate transporter, is strictly regulated by STOP1. Expression of the gene is activated by Al, but is also activated by bacterial elicitors. The former protects root tips from Al toxicity, but the latter recruites veneficial bacteria that induce plant immunity. It indecated that STOP1 and regulating genes are likely a module that has multiple function in stress registance of higher plants.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (30 results)

All 2014 2013 2012 2011 2010

All Journal Article (18 results) (of which Peer Reviewed: 15 results) Presentation (8 results) Book (4 results)

  • [Journal Article] Identification of a STOP1-like protein in Eucalyptus that regulates transcription of Al tolerance genes2014

    • Author(s)
      Sawaki Y., Kobayashi Y., Kihara-Doi T., Nishikubo N., Kawazu T., Kobyashi M., Kobayashi Y., Iuchi S., Koyama H.. & Sato S.
    • Journal Title

      Plant Sci.

      Volume: 223 Pages: 8-15

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] STOP2 activates transcription of several genes for Al- and low pH-tolerance that are regulated by STOP1 in Arabidopsis2014

    • Author(s)
      Kobayashi, Y., Ohyama, Y., Kobayashi, Y., Ito, H., Iuchi, S., Fujita, M., Zhao, C-R., Tanveer, T., Ganesan, M., Kobayashi, M. & Koyama H.
    • Journal Title

      Mol Plant

      Volume: 7 Pages: 311-322

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Al-responsive citrate excretion and citrate-transporting MATEs in Eucalyptus camaldulensis2013

    • Author(s)
      Sawaki,Y., Kihara-Doi, T., Kobayashi, Y., Nishikubo, N., Kawazu, T., Kobayashi, Y., Koyama, H. & Sato S.
    • Journal Title

      Planta

      Volume: 237

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the complex regulation of AtALMT1 expression in response to phytohormones and other inducers2013

    • Author(s)
      Kobayashi, Y. Kobayashi, Y., Sugimoto, M., Lakshmanan, V., Iuchi, S., Kobayashi, M., Bais, H and Koyama, H.
    • Journal Title

      Plant Physiol

      Volume: 162 Pages: 732-740

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of AtALMT1 in the Arabidopsis thaliana ecotype Columbia results in enhanced Al-activated malate excretion and beneficial bacterium recruitment2013

    • Author(s)
      Kobayashi, Y., Lakshmanan, V., Kobayashi, Y., Asai, M., Iuchi, S., Kobayashi, M., Bais, H. P. & Koyama H.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 8(9)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of AtSTOP1 orthologous genes in tobacco and other plant species2013

    • Author(s)
      Ohyama, Y., Ito, H., Kobayashi, Y., Ikka, T., Morita, A., Kobayashi, M., Imaizumi, R., Aoki, T., Komatsu, K., Sakata, Y., Iuchi, S. & Koyama, H.
    • Journal Title

      Plant Physiol

      Volume: 162 Pages: 1937-1946

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and physiological analysis of Al3+ and H+ rhizotoxicities at moderately acidic conditions2013

    • Author(s)
      Kobayashi, Y., Kobayashi, Y., Watanabe, T., Shaff J. E., Ohta, H., Kochian, L. V., Wagatsuma, T., Kinraide, T. B. & Koyama H.
    • Journal Title

      Plant Physiol

      Volume: 163 Pages: 180-192

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of the complex regulation of AtALMT1 expression in response to phytohormones and other inducers.2013

    • Author(s)
      Kobayashi, Y. Kobayashi, Y., Sugimoto, M., Lakshmanan, V., Iuchi, S., Kobayashi, M., Bais, H and Koyama, H.
    • Journal Title

      Plant Physiology

      Volume: 162 Issue: 2 Pages: 732-740

    • DOI

      10.1104/pp.113.218065

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of AtALMT1 in the Arabidopsis thaliana ecotype Columbia results in enhanced Al-activated malate excretion and beneficial bacterium recruitment.2013

    • Author(s)
      Kobayashi, Y., Lakshmanan, V., Kobayashi, Y., Asai, M., Iuchi, S., Kobayashi, M., Bais, H. P. & Koyama H.
    • Journal Title

      Plant Signaling & Behavior

      Volume: 8 Issue: 9 Pages: e25565-e25565

    • DOI

      10.4161/psb.25565

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of AtSTOP1 orthologous genes in tobacco and other plant species.2013

    • Author(s)
      Ohyama, Y., Ito, H., Kobayashi, Y., Ikka, T., Morita, A., Kobayashi, M., Imaizumi, R., Aoki, T., Komatsu, K., Sakata, Y., Iuchi, S. & Koyama, H.
    • Journal Title

      Plant Physiol.

      Volume: 162 Issue: 4 Pages: 1937-1946

    • DOI

      10.1104/pp.113.218958

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of Al-responsive citrate excretion and citrate-transporting MATEs in Eucalyptus camaldulensis.2013

    • Author(s)
      Sawaki,Y., Kihara-Doi, T., Kobayashi, Y., Nishikubo, N., Kawazu, T., Kobayashi, Y., Koyama, H. & Sato S.
    • Journal Title

      Planta

      Volume: 237 Issue: 4 Pages: 979-989

    • DOI

      10.1007/s00425-012-1810-z

    • Related Report
      2013 Annual Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular and physiological analysis of Al3+ and H+ rhizotoxicities at moderately acidic conditions.2013

    • Author(s)
      Kobayashi, Y., Kobayashi, Y., Watanabe, T., Shaff J. E., Ohta, H., Kochian, L. V., Wagatsuma, T., Kinraide, T. B. & Koyama
    • Journal Title

      Plant Physiol

      Volume: 163 Issue: 1 Pages: 180-92

    • DOI

      10.1104/pp.113.222893

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 他種由来STOP1 相同遺伝子を導入した相補組換え体シロイヌナズナの解析2012

    • Author(s)
      大山慶直,小林佑理子,井内聖,小山博之
    • Journal Title

      無菌生物

      Volume: 42 Pages: 69-72

    • NAID

      10031131373

    • Related Report
      2013 Final Research Report
  • [Journal Article] Analysis of environmental stress in plants with the aid of marker genes for H2O2 responses2012

    • Author(s)
      Hieno, A., Naznin, A.H. Sawaki, K., Koyama, H., Sakai, S., Ishino, H., Hyakumachi, M. & Yamamoto, Y.Y.
    • Journal Title

      Methods Enzymol

      Volume: 527 Pages: 221-238

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] シロイヌナズナ相補組換えによるSTOP1相同遺伝子の機能解析2012

    • Author(s)
      大山義直, 他
    • Journal Title

      無菌生物

      Volume: 35(印刷中)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] シロイヌナズナをモデルとした植物の酸性土壌耐性に関する研究2011

    • Author(s)
      小山博之
    • Journal Title

      土肥誌

      Volume: 82 Pages: 349-352

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Prediction of transcriptional regulatory elements for plant hormone responses based on microarray data2011

    • Author(s)
      Yamamoto YY, Yoshioka Y, Hyakumachi M, Maruyama K, Yamaguchi-Shinozaki K, Tokizawa M, Koyama H
    • Journal Title

      BMC Plant Biology

      Volume: 39

    • NAID

      130006995311

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞膜表面のイオン活動度推定プログラムを用いたシロイヌナズナのアルミニウム耐性機構に関する研究2010

    • Author(s)
      小林安文, 小山博之
    • Journal Title

      無菌生物

      Volume: 40 Pages: 79-80

    • NAID

      10027130371

    • Related Report
      2010 Annual Research Report
  • [Presentation] STOP1 Regulating System ; A Root Module for Adapting to Acid Soil Environment, and for Enhancing Plant Immunity2013

    • Author(s)
      Koyama H., Kobayashi Y., Bais H, Iuchi S.
    • Organizer
      第3回国際農業会議
    • Place of Presentation
      中国杭州
    • Related Report
      2013 Final Research Report
  • [Presentation] リンゴ酸トランスポーターAtALMT1 活性化を介したリンゴ酸放出機構の解析2013

    • Author(s)
      小山博之
    • Organizer
      日本植物生理学会
    • Place of Presentation
      岡山
    • Related Report
      2012 Annual Research Report
  • [Presentation] 無菌栽培系を用いた植物の有機酸放出アルミニウム耐性機構の解明2011

    • Author(s)
      小林佑理子, 小山博之
    • Organizer
      無菌生物ノートバイオロジー学会
    • Place of Presentation
      札幌
    • Year and Date
      2011-01-13
    • Related Report
      2010 Annual Research Report
  • [Presentation] シロイヌナズナ根部イオン処理下で誘導される地上部遺伝子発現のプロファイリング2011

    • Author(s)
      澤木克亘, 他
    • Organizer
      土肥学会
    • Place of Presentation
      つくば市
    • Related Report
      2011 Annual Research Report
  • [Presentation] 共発現遺伝子ネットワークによるSTOP1変異の解析2011

    • Author(s)
      山中啓史, 他
    • Organizer
      土肥学会
    • Place of Presentation
      つくば市
    • Related Report
      2011 Annual Research Report
  • [Presentation] マイクロアレイデータを用いたイオンストレス転写制御配列の予測2011

    • Author(s)
      時澤睦朋, 他
    • Organizer
      土肥学会
    • Place of Presentation
      つくば市
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナをモデルとした植物の酸性土壌耐性機構に関する研究2011

    • Author(s)
      小山博之
    • Organizer
      土肥学会
    • Place of Presentation
      つくば市
    • Related Report
      2011 Annual Research Report
  • [Presentation] 細胞表面イオン活動度プログラムを用いたシロイヌナズナアルミニウム応答遺伝子の解析2010

    • Author(s)
      小林安文, 小山博之, 他
    • Organizer
      日本土壌肥料学会
    • Place of Presentation
      札幌
    • Year and Date
      2010-09-07
    • Related Report
      2010 Annual Research Report
  • [Book] Rhizotoxic ions : 'Omics' approaches for studying abiotic stress tolerance in plants. In Omics and Plant Abiotic Stress Tolerance2011

    • Author(s)
      Zhao, C-Z., Yamamoto, Y.Y. & Koyama, H.
    • Related Report
      2013 Final Research Report
  • [Book] Heavy metal tolerance in Arabidopsis thaliana Advances in Botanical Research 602011

    • Author(s)
      Chaffai, R. & Koyama, H.
    • Related Report
      2013 Final Research Report
  • [Book] Advance in Botanical Reserch (Chapter 1 Heavy Metal Tolerance in Arabidopsis thaliana)2011

    • Author(s)
      Chaffai RB Koyama, H
    • Total Pages
      49
    • Publisher
      Academic Press
    • Related Report
      2011 Annual Research Report
  • [Book] Advances in Botanical Research2011

    • Author(s)
      Chaffai, R, Koyama, H
    • Publisher
      Heavy metal tolerance in Arabidopsis thaliana(印刷中)
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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