| Budget Amount *help |
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2012: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2011: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2010: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
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| Research Abstract |
(1) Transcription factor AmyR was localized to nucleus and significantly unstable in the presence of inducer, but MalR showed constitutive nuclear localization and stability irrespective of carbon sources. AmyR was associated with chaperons Hsp70 and Hsp90. (2) Maltose transporter MalP was targeted to the vacuole after addition of glucose. Both HECT ubiquitin ligase HulA and arrestin-like protein CreD are required for glucose-induced endocytosis of MalP. In addition, pull-down analysis using CreD-3FLAG and WW domains of HulA fused to GST indicated that CreD could interact with HulA via WW domains. (3) Comprehensive screening of genes involved in pentose metabolism was performed by transcriptome analysis, and enzymatic properties of candidate gene products were analyzed, which resulted in the complete elucidation of the pentose metabolic pathway in Aspergillus oryzae.
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