| Budget Amount *help |
¥44,460,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥10,260,000)
Fiscal Year 2013: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2012: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Fiscal Year 2011: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2010: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
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| Outline of Final Research Achievements |
In recent years, the development of bio-fuel buttery using unutilized bioresources like food wastes have been interesting. In this project, we have been studied the construction of enzyme buttery (include microbial buttery) using highly active and long-term stable oxidoreductases as the element in a fuel cell. As a result, we succeeded the construction of L-proline-enzyme buttery using thermostable due-linked L-proline dehydrogenase. This is the first success in the production of amino acid enzyme buttery. In order to improve the battery cell unit for the increase of its output, we have screened more active and novel oxidoreductases based on both genome information and activity based methods, and succeeded in the finding of several stable enzymes including thermostable glutamate dehydrogenase and D-amino acid dehydrogenase.
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