|Budget Amount *help
¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2013: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2012: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Fiscal Year 2011: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2010: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
In order to get new insights on physiological functions of small peptides, their potentials in tissues such as vessels and muscles were investigated in this study. As a result, it was clarified that basic di-peptides could relax contracted thoraces aorta in an endothelium-independent manner through the suppression of Ca2+-related signaling pathways by cell -line experiments and in silica analyses. Furthermore, we have demonstrated for the first time that the potent vasorelaxation power of basic do-peptides was ameliorated with aging and hypertension. We further got new evidence that basic di-peptides could absorb intact across intestinal membrane by our proposed new visualization technique (MADL-MS), and show anti-diabetic effect in muscle cells.