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Study on fish osmoregulation and its application

Research Project

Project/Area Number 22248021
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionThe University of Tokyo

Principal Investigator

KANEKO TOYOJI  東京大学, 農学生命科学研究科, 教授 (70221190)

Co-Investigator(Renkei-kenkyūsha) LEE Kyung Mi  東京大学, 農学生命科学研究科, 特任研究員 (30507885)
WATANABE Soichi  東京大学, 農学生命科学研究科, 助教 (20507884)
Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2011: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2010: ¥22,360,000 (Direct Cost: ¥17,200,000、Indirect Cost: ¥5,160,000)
Keywords浸透圧調節 / ティラピア / 塩類細胞 / カリウム / セシウム / 温泉トラフグ / アミノ酸輸送体 / イオン輸送体 / ROMK / ROMK / ライフサイクル / 機能の可塑性 / NCC / NHE3 / CFTR / NKCC / トラフグ / 遊離アミノ酸 / 血漿浸透圧 / コラーゲン / 温泉
Research Abstract

In the present study, we aimed to clarify molecular and cellular mechanisms of fish osmoregulation, and seek a possibility to apply fish osmoregulatory studies to fisheries industry. First, we clarified the functional plasticity and life cycle of gill ionocytes in tilapia. Second, gill ionocytes were demonstrated to excrete potassium, and the molecular mechanism of potassium excretion was shown in tilapia. Cesium was also secreted from gill ionocytes through the potassium-excreting mechanism. Finally, the involvement of free amino acids in intracellular osmoregulation was shown in tilapia and puffer fish. It is highly possible that temporal exposure of fish to higher salinity water causes a transient increase in intracellular amino acid concentration, resulting in a transient increase in fish meat taste.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (46 results)

All 2014 2013 2012 2011 2010

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (20 results) (of which Invited: 5 results) Book (6 results)

  • [Journal Article] Bicarbonate secreted from the pancreas contributed to the formation of Ca precipitates in Japanese eel, Anguilla Japonica.2013

    • Author(s)
      Mekuchi, M., Watanabe, S. and Kaneko, T.
    • Journal Title

      Journal of Experimental Zoology - PartA

      Volume: 319 Issue: 1 Pages: 53-62

    • DOI

      10.1002/jez.1774

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hyposmoregulatory ability and ionand water-regulatory mechanisms during the leptocephalus stages of Japanese eel Anguilla japonica2013

    • Author(s)
      Lee K. M., Yamada Y., Okamura A., Tsukamoto K., Kaneko T. (他2名)
    • Journal Title

      Fisheries Science

      Volume: vol.79 Issue: 1 Pages: 77-86

    • DOI

      10.1007/s12562-012-0576-3

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression of sodium/hydrogen exchanger 3 and cation-chloride cotransporters in the kidney of Japanese eel acclimated to a wide range of salinities2013

    • Author(s)
      Keitaro Teranishi, Miyuki Mekuchi, Toyoji Kaneko
    • Journal Title

      Comparative Biochemistry and Physiology, Part A

      Volume: 164 Issue: 2 Pages: 333-343

    • DOI

      10.1016/j.cbpa.2012.11.011

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of cardiac natriuretic peptides in seawater adaptation of medaka embryos as revealed by loss-of-function analysis2013

    • Author(s)
      Miyanishi, H., Okubo, K., Kaneko, T., and Takei, Y.
    • Journal Title

      Am. J. Physiol. Regul. Integr.Comp. Physiol

      Volume: 304 Issue: 6 Pages: R423-R434

    • DOI

      10.1152/ajpregu.00384.2012

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expressions of ion transporters in gill mitochondrion-rich cells in Japanese eel acclimated to a wide range of environmental salinity.2013

    • Author(s)
      Seo, M.Y., Mekuchi, M., Teranishi, K. and Kaneko, T.
    • Journal Title

      Comparative Biochemistry and Physiology - Part A

      Volume: 166 Issue: 2 Pages: 323-332

    • DOI

      10.1016/j.cbpa.2013.07.004

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization and gene expression of Na+-K+-2Cl- cotransporter2 (NKCC2) in the gastrointestinal tract of Olive flounder (Paralichythys olivaceus) during the four days after infection with Streptococcus parauberis2013

    • Author(s)
      Kim, Y. K., Watanabe, S., Park, S. I., Jeong, J. B., Kaneko, T., Park, M. A., and Yeo, I. K.
    • Journal Title

      Mar. Freshw. Behav. Physiol.

      Volume: 46 Issue: 3 Pages: 145-157

    • DOI

      10.1080/10236244.2013.793472

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intestinal expression of peptide transporter 1 (PEPT1) at different life stages of Japanese eel, Anguilla japonica.2013

    • Author(s)
      Ahn, H., Yamada, Y., Okamura, A., Tsukamoto, K., Kaneko, T. and Watanabe, S.
    • Journal Title

      Comparative Biochemistry and Physiology - Part B

      Volume: 166 Issue: 2 Pages: 157-164

    • DOI

      10.1016/j.cbpb.2013.08.005

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potassium excretion through ROMK potassium channel expressed in gill mitochondrion-rich cells of Mozambique tilapia2012

    • Author(s)
      古川史也
    • Journal Title

      American Journal of Physiology-Regulatory, Integrative and comparative physiolgy

      Volume: 302 Issue: 5 Pages: R568-R576

    • DOI

      10.1152/ajpregu.00628.2011

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Excretion of cesium and rubuduum via the branchial potassium-transporting pathway in Mozambique tilapia2012

    • Author(s)
      古川史也
    • Journal Title

      Fisheries Science

      Volume: 78 Issue: 3 Pages: 597-602

    • DOI

      10.1007/s12562-012-0492-6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Recruitment and degeneration of mitochondrion-rich cells in the gills of Mozambique tilapia Oreochromis mossambicus during adaptation to a hyperosmotic environment2012

    • Author(s)
      Mayu Inokuchi and Toyoji Kaneko
    • Journal Title

      Comparative Biochemistry and Physiology, PartA

      Volume: 162 Issue: 3 Pages: 245-251

    • DOI

      10.1016/j.cbpa.2012.03.018

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Differential regulation of TRPV4 mRNA levels by acclimation salinity and extracellular osmolality in euryhaline tilapia2012

    • Author(s)
      Seale, A. P., Watanabe, S., Breves, J.P., Lerner, D. T., Kaneko, T., and Grau, E.G.
    • Journal Title

      Gen. Comp. Endocrinol.

      Volume: 178 Issue: 1 Pages: 123-130

    • DOI

      10.1016/j.ygcen.2012.04.020

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Morphofunctional modifications in gill mitochondria-rich cells of Mozambique tilapia transferred from freshwater to 70% seawater, detected by dual observations of whole-mount immunocytochemistry and scanning electron microscopy2011

    • Author(s)
      Choi, J.H., Lee, K.M., Inokuchi, M., and Kaneko, T.
    • Journal Title

      Comp. Biochem. Physiol

      Volume: A 158 Issue: 1 Pages: 132-142

    • DOI

      10.1016/j.cbpa.2010.09.019

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] NKCC2β and NCCβ expressed in the intestine and rectum, respectively, in Japanese eel Anguilla japonica2011

    • Author(s)
      Watanabe, S., Mekuchi, M., Ideuchi, H., Kim, Y. K., and Kaneko, T.
    • Journal Title

      Comp. Biochem. Physiol

      Volume: A 159 Issue: 4 Pages: 427-435

    • DOI

      10.1016/j.cbpa.2011.04.009

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Morphofunctional modifications in gill mitochondria-rich cells of Mozambique tilapia transferred from freshwater to 70% seawater, detected2011

    • Author(s)
      Choi, J.H., Lee, K.M., Inokuchi, M., Kaneko, T.
    • Journal Title

      Comp.Biochem.Physiol.A

      Volume: 158 Pages: 132-142

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Responses of gill mitochondria-rich cell in Mozambique tilapia acclimated to acidic environments (pH4.0) in combination with different2011

    • Author(s)
      Furukawa, F., Watanabe, S., Inokuchi, M., Kaneko, T.
    • Journal Title

      Comp.Biochem.Physiol.A

      Volume: 158 Pages: 468-476

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Electroneutral cation-Cl^-cotransporters NKCC2β and NCCβ expressed in the intestine and rectum, respectively, in Japanese eel Anguilla japonica2011

    • Author(s)
      Watanabe, S., Mekuchi, M., Ideuchi, H., Kim, Y.K., Kaneko, T.
    • Journal Title

      Comp.Biochem.Physiol.A

      Volume: 159 Pages: 427-435

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mg-calcite, a carbonate mineral, constitutes Ca precipitates produced as a byproduct of osmoregulation in the intestine of seawater-acclimated Japanese eel Anguilla japonica2010

    • Author(s)
      Mekuchi, M., Hatta, T., and Kaneko, T.
    • Journal Title

      Fish. Sci

      Volume: 76 Issue: 2 Pages: 199-205

    • DOI

      10.1007/s12562-009-0199-5

    • NAID

      10026162962

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spatial, cellular and intracellular localization of Na^+/K^+-ATPase in the sterically-disposed renal tubules of Japanese eel2010

    • Author(s)
      Teranishi, K., and Kaneko, T.
    • Journal Title

      J. Histochem. Cytochem

      Volume: 58 Issue: 8 Pages: 707-719

    • DOI

      10.1369/jhc.2010.955492

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prolactin-releasing peptide receptor expressed in the pituitary in Mozambique tilapia Oreochromis mossambicus : an aspect of prolactin regulatory mechanisms2010

    • Author(s)
      Watanabe, S, Kaneko, T.
    • Journal Title

      Gen.Comp.Endocrinol.

      Volume: 167 Pages: 27-34

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatial, cellular and intracellular localization of Na^+/K^+-ATPase in the sterically-disposed renal tubules of Japanese eel2010

    • Author(s)
      Teranishi, K., Kaneko, T.
    • Journal Title

      J.Histochem.Cytochem.

      Volume: 58 Pages: 707-719

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 機能と形態から探る魚類生理学2014

    • Author(s)
      金子豊二
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      函館
    • Related Report
      2013 Final Research Report
  • [Presentation] 機能と形態から探る魚類生理学(平成26年度日本水産学会賞受賞講演)2014

    • Author(s)
      金子豊二
    • Organizer
      平成26年度日本水産学会春季大会
    • Place of Presentation
      北海道大学水産学部(北海道)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 海が嫌いなキンギョの話2013

    • Author(s)
      金子豊二
    • Organizer
      東京大学オープンキャンパス2013農学部コース
    • Place of Presentation
      東京大学農学部,東京
    • Year and Date
      2013-08-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 水産動物におけるセシウムの取り込みと排出2013

    • Author(s)
      金子豊二
    • Organizer
      公益財団法人農学会・日本農学アカデミー共同主催シンポジウム,放射能汚染の不安に答える-水産物はどこまで安全か-
    • Place of Presentation
      東京大学弥生講堂,東京
    • Year and Date
      2013-02-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 魚類におけるセシウムの動態を探る2013

    • Author(s)
      金子豊二
    • Organizer
      第38回日本比較内分泌学会大会
    • Place of Presentation
      宮崎市民プラザ,宮崎
    • Related Report
      2013 Final Research Report
  • [Presentation] 海水魚におけるセシウムの取込みと排出2013

    • Author(s)
      金子豊二
    • Organizer
      第2回環境放射能除染研究発表会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 魚類におけるセシウムの動態を探る2013

    • Author(s)
      金子豊二
    • Organizer
      第38回日本比較内分泌学会大会
    • Place of Presentation
      宮崎市民プラザ(宮崎県)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 水産動物におけるセシウムの取り込みと排出2013

    • Author(s)
      金子豊二
    • Organizer
      公益財団法人農学会・日本農学アカデミー共同主催シンポジウム,放射能汚染の不安に答える-水産物はどこまで安全か-
    • Place of Presentation
      東京大学弥生講堂
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 海水魚におけるセシウムの取込みと排出2012

    • Author(s)
      金子豊二
    • Organizer
      第四回放射能の農畜水産物等への影響についての研究報告会-東日本大震災に関する救援・復興に係る農学生命科学研究科の取組み-
    • Place of Presentation
      東京大学弥生講堂・一条ホール,東京
    • Year and Date
      2012-09-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 海水魚のエラからセシウムが排出される2012

    • Author(s)
      金子豊二
    • Organizer
      第三回放射能の農畜水産物等への影響についての研究報告会-東日本大震災に関する救援・復興に係る農学生命科学研究科の取組み-
    • Place of Presentation
      東京大学安田講堂,東京
    • Year and Date
      2012-05-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 海水魚におけるセシウムの取込みと排出2012

    • Author(s)
      金子豊二
    • Organizer
      第四回放射能の農畜水産物等への影響についての研究報告会-東日本大震災に関する救援・復興に係る農学生命科学研究科の取組み-
    • Place of Presentation
      東京大学弥生講堂
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] ウナギ体表の被蓋細胞によるイオン調節の可能性2012

    • Author(s)
      徐 美暎・金子豊二
    • Organizer
      平成24年度日本水産学会秋季大会
    • Place of Presentation
      下関
    • Related Report
      2012 Annual Research Report
  • [Presentation] ウナギ腎臓における1価イオン輸送体の局在とその役割2012

    • Author(s)
      寺西慶太郎・馬久地みゆき・金子豊二
    • Organizer
      平成24年度日本水産学会秋季大会
    • Place of Presentation
      下関
    • Related Report
      2012 Annual Research Report
  • [Presentation] 環境浸透圧によるモザンビークティラピアの腸管アミノ酸吸収機構の変化2012

    • Author(s)
      相馬智史・金子豊二・渡邊壮一
    • Organizer
      平成24年度日本水産学会秋季大会
    • Place of Presentation
      下関
    • Related Report
      2012 Annual Research Report
  • [Presentation] Differentiation and apoptosis of mitochondria-rich cells in gills of Mozambique tilapia2011

    • Author(s)
      Inokuchi, M., Kaneko, T.
    • Organizer
      8th International Congress on Comparative Physiology and Biochemistry
    • Place of Presentation
      名古屋
    • Year and Date
      2011-05-02
    • Related Report
      2011 Annual Research Report
  • [Presentation] ティラピア鰓におけるK^+排出機構の解明とRb^+,Cs^+輸送の検討2011

    • Author(s)
      古川史也・渡辺壮一・金子豊二
    • Organizer
      平成24年度日本水産学会秋季大会
    • Place of Presentation
      長崎
    • Related Report
      2013 Final Research Report
  • [Presentation] Differentiation and apoptosis of mitochondria-rich cells in gills of Mozambique tilapia2011

    • Author(s)
      Inokuchi, M., and Kaneko, T.
    • Organizer
      8th International Congress on Comparative Physiology and Biochemistry
    • Place of Presentation
      Nagoya, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 広塩性魚における環境浸透圧変化による筋肉中遊離アミノ酸量変動と呈味への影響2010

    • Author(s)
      坂本安弘・渡邊壮一・山口洋子・金子元・渡部終五・金子豊二
    • Organizer
      平成22年度日本水産学会秋季大会
    • Place of Presentation
      京都
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] 酸性環境に曝露したティラピアにおける塩類細胞の応答2010

    • Author(s)
      古川史也・渡辺壮一・井ノ口繭・金子豊二
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      藤沢
    • Related Report
      2013 Final Research Report
  • [Presentation] ティラピアを淡水から脱イオン水に移行後した際の鰓塩類細胞の経時的変化2010

    • Author(s)
      崔 丁玄・李 慶美・井ノ口 繭・金子豊二
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      藤沢
    • Related Report
      2013 Final Research Report
  • [Book] Excretion of cesium through potassium transport pathway in the gills of a marine teleost. In "Agricultural Implications of the Fukushima Nuclear Accident" (Nakanishi, T. M. and Tanoi, K. Eds)2013

    • Author(s)
      Kaneko, T., Furukawa, F., and Watanabe, S.
    • Publisher
      Springer
    • Related Report
      2013 Final Research Report
  • [Book] 増補改訂版 魚類生理学の基礎2013

    • Author(s)
      会田勝美・金子豊二 編
    • Total Pages
      265
    • Publisher
      恒星社厚生閣
    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Annual Research Report
  • [Book] Excretion of cesium through potassium transport pathway in the gills of a marine teleost. In “Agricultural Implications of the Fukushima Nuclear Accident” (Nakanishi, T. M. and Tanoi, K. Eds)2013

    • Author(s)
      Kaneko, T., Furukawa, F., and Watanabe, S.
    • Total Pages
      204
    • Publisher
      Springer
    • Related Report
      2012 Annual Research Report
  • [Book] ウナギの塩分濃度適応戦略,ウナギの博物誌(黒木真理 編著)2012

    • Author(s)
      金子豊二
    • Publisher
      化学同人
    • Related Report
      2013 Final Research Report
  • [Book] 最新 水産ハンドブック2012

    • Author(s)
      金子豊二
    • Publisher
      講談社サイエンティフィク
    • Related Report
      2013 Final Research Report
  • [Book] ウナギの塩分濃度適応戦略,ウナギの博物誌(黒木真理 編著)2012

    • Author(s)
      金子豊二
    • Total Pages
      254
    • Publisher
      化学同人
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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