Development of comparative metagenomics for soil microbiome analysis
| Project/Area Number |
22248038
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SENOO Keishi 東京大学, 大学院・農学生命科学研究科, 教授 (40206652)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Hiroyuki 茨城大学, 農学部, 教授 (80168947)
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| Co-Investigator(Renkei-kenkyūsha) |
HATTORI Masahira 東京大学, 新領域創成科学研究科, 教授 (70175537)
SHINANO Takuro (独)農業・食品産業技術総合研究機構, 東北農業研究センター 農業放射線研究 センター, センター長 (20235542)
MINAMISAWA Kiwamu 東北大学, 大学院・生命科学研究科, 教授 (70167667)
OTSUKA Shigeto 東京大学, 大学院・農学生命科学研究科, 准教授 (10313074)
HASHIMOTO Tomoyoshi (独)農業・食品産業技術総合研究機構, 中央農業総合研究センター, 上席研究員 (90343946)
YOH Muneoki 東京農工大学, 農学研究院, 教授 (50260526)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2012: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2011: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2010: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000)
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| Keywords | 生物環境 / 土壌メタゲノミクス / 土壌環境再生 / 窒素循環 / 水田土壌 / メタトランスクリプトーム解析 / 畑土壌 / 三宅島森林土壌 / 細菌群集構造 / 糸状菌群集構造 / 一酸化二窒素 / RNAクローンライブラリー解析 / 定量PCR解析 / Rice cluster VI / 初成土壌 / Leptosprillum属細菌 / Acidithiobacillus属細菌 / RubisCO |
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| Research Abstract |
We tried to uncover the structure and function of microbial communities in soil ecosystem through soil metagenomics using the new DNA-sequencing technology. Novel microbial communities responsible for reduction reaction and nitrogen transformation in rice paddy soil were revealed. The resilience of soil microbial community to the volcanic eruption impact was revealed and the detailed picture of soil microbial genetic response to the long-term application of livestock liquid manure was obtained.
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Report
(4 results)
Research Products
(66 results)
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[Presentation] Bacterial community of the fresh volcanic deposits on barren land, grassland, and woodland in the Island of Miyake, Japan, as revealed by pyrosequencing-based analysis of 16S rRNA genes2012
Author(s)
Yong Guo, Reiko Fujimura, Yoshinori Sato, Kenshiro Oshima, Masahira Hattori, Takashi Kamijo, Hiroyuki Ohta
Organizer
14th International Symposium on Microbial Ecology
Place of Presentation
コペンハーゲン、デンマーク
Year and Date
2012-08-21
Related Report
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[Presentation] Metagenomic analysis of microbial succession on fresh volcanic ash deposits in the Island of Miyake2011
Author(s)
Reiko Fujimura, Seokwon Kim, Yong Guo, Yoshinori Sato, Ken Hosokawa, Wataru Suda, Erika Iioka, Kenshiro Ohshima, Masahira Hattori, Takashi Kamijo and Hiroyuki Ohta
Organizer
Japan, 20th International Symposium on Environmental Biogeochemistry
Place of Presentation
イスタンブール工科大学,イスタンブール
Year and Date
2011-09-28
Related Report
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[Presentation] NITROGEN FIXATION BY IRON-OXIDIZING BACTERIA DURING EARLY ECOSYSTEM DEVELOPMENT ON MIYAKE-JIMA2010
Author(s)
Reiko Fujimura, Yoshinori Sato, Tomonori Sugawara, Ken Hosokawa, Tomoyasu Nishizawa, Kenji Nanba, Takashi Kamijo, Bill Holben and Hiroyuki Ohta
Organizer
JAPAN, 13th International Symposium on Microbial Ecology
Place of Presentation
Seattle, WA, USA
Year and Date
2010-08-26
Related Report
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[Presentation] NITROGEN FIXATION BY IRON-OXIDIZING BACTERIA DURING EAELY ECOSYSTEM DEVELOPMENT ON MIYAKE-JIMA, JAPAN VOLCANIC DEPOSITS2010
Author(s)
Reiko Fujimura, Yoshinori Sato, Tomonori Sugawara, Ken Hosokawa, Tomoyasu Nishizawa, Kenji Nanba, Takashi Kamijo, Bill Holben, Hiroyuki Ohta
Organizer
13th International Symposium on Microbial Ecology
Place of Presentation
Seattle, WA, USA
Year and Date
2010-08-26
Related Report
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[Presentation] Bacterial community of the fresh volcanic deposits on barren land, grassland, and woodland in the Island of Miyake, Japan, as revealed by pyrosequencing-based analysis of 16S rRNA genes
Author(s)
Yong Guo, Reiko Fujimura, Yoshinori Sato, Kenshiro Oshima, Masahira Hattori, Takashi Kamijo, Hiroyuki Ohta
Organizer
14th International Symposium on Microbial Ecology
Place of Presentation
ベラ，センター，コペンハーゲン
Related Report
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[Presentation] Metagenomic analysis of microbial succession on fresh volcanic ash deposits in the Island of Miyake, Japan
Author(s)
Reiko Fujimura, Seokwon Kim, Yong Guo, Yoshinori Sato, Ken Hosokawa, Wataru Suda, Erika Iioka, Kenshiro Ohshima, Masahira Hattori, Takashi Kamijo and Hiroyuki Ohta
Organizer
20th International Symposium on Environmental Biogeochemistry
Place of Presentation
イスタンブール工科大学，イスタンブール
Related Report
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