| Budget Amount *help |
¥48,750,000 (Direct Cost: ¥37,500,000、Indirect Cost: ¥11,250,000)
Fiscal Year 2012: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2011: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2010: ¥22,750,000 (Direct Cost: ¥17,500,000、Indirect Cost: ¥5,250,000)
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| Research Abstract |
In order to examine the functional roles of translesion synthesis (TLS) in mammalian cells, we have established many mouse embryonic fibroblast cell lines expressing polymerase activity-defective or Rev1 interaction-defective mutant DNA polymerase eta (Pol eta) in Pol eta, Pol iota or Pol kappa single-, double- or triple-deficient mouse embryonic fibroblasts (MEFs). Using these MEF cells, we investigated UV sensitivity as well as mutation frequency upon UV irradiation. Our results suggest that there is an error-prone TLS pathway that uses Pol kappa via Rev1 in the absence of Pol eta.
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