| Project/Area Number |
22249038
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Gunma University |
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Principal Investigator |
MORI Masatomo 群馬大学, 大学院・医学系研究科, 教授 (80174382)
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| Co-Investigator(Kenkyū-buntansha) |
OKADA Shuichi 群馬大学, 医学部, 講師 (20260474)
HASHIMOTO Koshi 群馬大学, 大学院・医学系研究科, 助教 (30396642)
YADA Toshihiko 自治医科大学, 医学部, 教授 (60166527)
YAMADA Masanobu 群馬大学, 大学院・医学系研究科, 准教授 (90261833)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000)
Fiscal Year 2012: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
Fiscal Year 2011: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2010: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
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| Keywords | Nesfatin-1 / 肥満 / シグナル伝達 / 摂食抑制 / 脂肪細胞 / ネスファチン-1 / アピトサイトカイン / 遺伝子 / メラノコルチン受容体 / リン酸化CRE結合蛋白(p-CREB) / 膵β細胞 / 視床下部 / G蛋白共役型受容体 / ジーンターゲティング / 受容体 / サイクリックAMP |
|---|
| Research Abstract |
Nesfatin-1 administration promoted a dose-dependent increase in CRE reporteractivities inmouse NB41A3cells,and Nesfatin-1 bound plasmamembranes derived frommouse hypothalami and NB41A3 cells in a high affinity manner. Using a CRE reporter assay ineachof 49 GPCRs, wetried to identify a receptorspecific to Nesfatin-1, but these efforts resulted in failure. Troglitazone, an activator for PPARγ, possessed a significantstabilizing activity of NUCB2 gene mRNA, and Nesfatin-1 activated EGF signaling tosuppress adipogenesis.
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