Study for the pathogenesis of autoimmune diseases using sphingomyeline knock out mouse
Project/Area Number |
22249041
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Research Category |
Grant-in-Aid for Scientific Research (A)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
膠原病・アレルギー・感染症内科学
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Research Institution | Kanazawa Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
OKAZAKI Kazuichi 関西医科大学, 医学部, 教授 (70145126)
OKAZAKI Toshiro 金沢医科大学, 医学部, 教授 (40233308)
WATANABE Ken 独立行政法人国立長寿医療研究センター, 運動器疾患研究部, 研究室リーダー (10342966)
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Co-Investigator(Renkei-kenkyūsha) |
KAWANAMI Takafumi 金沢医科大学, 医学部, 講師 (20350762)
FUJITA Yoshimasa 金沢医科大学, 医学部, 講師 (70450880)
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Project Period (FY) |
2010-04-01 – 2013-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥46,670,000 (Direct Cost: ¥35,900,000、Indirect Cost: ¥10,770,000)
Fiscal Year 2012: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2011: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2010: ¥20,150,000 (Direct Cost: ¥15,500,000、Indirect Cost: ¥4,650,000)
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Keywords | スフィンゴミエリン / リピッドラフト / ノックアウトマウス / 自己免疫性疾患 / 関節リウマチ / T細胞 / サイトカイン / コラーゲン誘導関節炎 |
Research Abstract |
We analyzed the pathogenesis of autoimmune diseases using sphingomyelin synthase 1 knockout (SMS1-KO) mouse. In vivo study showed that T cell function was impaired and cytokine production was decreased. By concanavalin A-induced hepatitis model, SMS1-KO mouse showed the amelioration of hepatitis. In addition, using collagen-induced arthritis model, we confirmed the amelioration of arthritis in SMS1-KO mouse. These results indicate that sphingomyelin has important roles in the pathogenesis of autoimmune diseases such as autoimmune hepatitis and rheumatoid arthritis through impaired functions of lipid rafts.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Long-term Heat Exposure Prevents Hypoxia-Induced Apoptosis in Mouse Fibroblast Cells2014
Author(s)
Sugimoto N, Shido O, Matsuzaki K, Katakura M, Hitomi Y, Tanaka M, Sawaki T, Fujita Y, Kawanami T, Masaki Y, Okazaki T, Nakamura H, Koizumi S, Yachie A and Umehara H
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Journal Title
Cell biochemistry and biophysics
Volume: (in press)
Issue: 1
Pages: 301-7
DOI
Related Report
Peer Reviewed
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[Journal Article] Deficient leptin signaling ameliorates systemic lupus erythematosus lesions in MRL/Mp-Fas lpr mice2014
Author(s)
Fujita Y, Fujii T, Mimori T, Sato T, Nakamura T, Iwao H, Nakajima A, Miki M, Sakai T, Kawanami T, Tanaka M, Masaki Y, Fukushima T, Okazaki T and Umehara H
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Journal Title
Journal of immunology
Volume: 192
Issue: 3
Pages: 979-984
DOI
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Peer Reviewed
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[Journal Article] Upregulation of aquaporin expression in the salivary glands of heat-acclimated rats2013
Author(s)
Sugimoto N, Matsuzaki K, Ishibashi H, Tanaka M, Sawaki T, Fujita Y, Kawanami T, Masaki Y, Okazaki T, Sekine J, Koizumi S, Yachie A, Umehara H and Shido O
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Journal Title
Scientific Reports
Volume: 3
Issue: 1
Pages: 1763-1763
DOI
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Peer Reviewed
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[Journal Article] Regulation of autophagy and its associated cell death by "sphingolipid rheostat": reciprocal role of ceramide and sphingosine 1-phosphate in the mammalian target of rapamycin pathway2012
Author(s)
Taniguchi M, Kitatani K, Kondo T, Hashimoto-Nishimura M, Asano S, Hayashi A, Mitsutake S, Igarashi Y, Umehara H, Takeya H, Kigawa J and Okazaki T
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Journal Title
The Journal of biological chemistry
Volume: 287
Pages: 39898-39910
URL
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Peer Reviewed
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[Journal Article] Cellular heat acclimation regulates cell growth, cell morphology, mitogen-activated protein kinase activation, and expression of aquaporins in mouse fibroblast cells2012
Author(s)
Sugimoto N, Shido O, Matsuzaki K, Ohno-Shosaku T, Hitomi Y, Tanaka M, Sawaki T, Fujita Y, Kawanami T, Masaki Y, Okazaki T, Nakamura H, Koizumi S, Yachie A and Umehara H
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Journal Title
Cell Physiol Biochem.
Volume: 30
Issue: 2
Pages: 450-457
DOI
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Peer Reviewed
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[Journal Article] Loss of HITS (FAM107B) expression in cancers of multiple organs : tissue microarray analysis2012
Author(s)
Nakajima H, Koizumi K, Tanaka T, Ishigaki Y, Yoshitake Y, Yonekura H, Sakuma T, Fukushima T, Umehara H, Ueno S, Minamoto T and Motoo Y
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Journal Title
International journal of oncology
Volume: 41
Issue: 4
Pages: 1347-1357
DOI
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Peer Reviewed
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[Journal Article] Induction of HITS, a newly identified family with sequence similarity 107 protein (FAM107B), in cancer cells by heat shock stimulation2012
Author(s)
Nakajima H, Ishigaki Y, Xia QS, Ikeda T, Yoshitake Y, Yonekura H, Nojima T, Tanaka T, Umehara H, Tomosugi N, Takata T, Shimasaki T, Nakaya N, Sato I, Kawakami K, Koizumi K, Minamoto T and Motoo Y
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Journal Title
International journal of oncology
Volume: 37
Pages: 583-593
URL
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Peer Reviewed
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[Journal Article] Follistatin-related protein/follistatin-like 1 evokes an innate immune response via CD14 and toll-like receptor 42012
Author(s)
Murakami K, Tanaka M, Usui T, Kawabata D, Shiomi A, Iguchi-Hashimoto M, Shimizu M, Yukawa N, Yoshifuji H, Nojima T, Ohmura K, Fujii T, Umehara H and Mimori T
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Journal Title
FEBS Lett.
Volume: 586
Pages: 319-324
URL
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Peer Reviewed
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[Journal Article] Skewed production of IL-6 and TGFbeta by cultured salivary gland epithelial cells from patients with Sjogren's syndrome2012
Author(s)
Kawanami T, Sawaki T, Sakai T, Miki M, Iwao H, Nakajima A, Nakamura T, Sato T, Fujita Y, Tanaka M, Masaki Y, Fukushima T, Hirose Y, Taniguchi M, Sugimoto N, Okazaki T and Umehara H
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Journal Title
PLoS One
Volume: 7
Issue: 10
Pages: e45689-e45689
DOI
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[Journal Article] CD4+ T-cell dysfunctions through the impaired lipid rafts ameliorate concanavalin A-induced hepatitis in sphingomyelin synthase 1-knockout mice2012
Author(s)
Dong L, Watanabe K, Itoh M, Huan CR, Tong XP, Nakamura T, Miki M, Iwao H, Nakajima A, Sakai T, Kawanami T, Sawaki T, Masaki Y, Fukushima T, Fujita Y, Tanaka M, Yano M, Okazaki T and Umehara H
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Journal Title
International immunology
Volume: 24
Issue: 5
Pages: 327-337
DOI
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Peer Reviewed
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[Journal Article] Regulation of cell migration by sphingomyelin synthases : sphingomyelin in lipid rafts decreases responsiveness to signaling by the CXCL12/CXCR4 pathway2012
Author(s)
Asano S, Kitatani K, Taniguchi M, Hashimoto M, Zama K, Mitsutake S, Igarashi Y, Takeya H, Kigawa J, Hayashi A, Umehara H and Okazaki T
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Journal Title
Molecular and cellular biology
Volume: 32
Issue: 16
Pages: 3242-3252
DOI
Related Report
Peer Reviewed
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[Journal Article] Cellular heat acclimation regulates cell growth, cell morphology, mitogen-activated protein kinase activation, and expression of aquaporins in mouse fibroblast cells2012
Author(s)
Sugimoto N, Shido O, Matsuzaki K, Ohno-Shosaku T, Hitomi Y, Tanaka M, Sawaki T, Fujita Y, Kawanami T, Masaki Y, Okazaki T, Nakamura H, Koizumi S, Yachie A and Umehara H
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Journal Title
Cell Physiol Biochem
Volume: 30
Pages: 450-457
Related Report
Peer Reviewed
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