Basic and clinical research on recovery of vision by electrical stimulation

• Project/Area Number: 22249058
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Osaka University
• Principal Investigator: FUJIKADO Takashi 大阪大学, 医学(系)研究科(研究院), 教授 (50243233)
• Co-Investigator(Kenkyū-buntansha): 森本 壮 大阪大学, 医学(系)研究科(研究院), 准教授 (00530198) ; 瓶井 資弘 大阪大学, 医学(系)研究科(研究院), 准教授 (40281125) ; 松下 賢治 大阪大学, 医学(系)研究科(研究院), 講師 (40437405) ; 神田 寛行 大阪大学, 医学(系)研究科(研究院), 助教 (50570248) ; 三好 智満 大阪大学, 医学(系)研究科(研究院), 助教 (70314309) ; 辻川 元一 大阪大学, 医学(系)研究科(研究院), 教授 (70419472) ; 坂口 裕和 大阪大学, 医学(系)研究科(研究院), 講師 (80379172) ; 藤田 克昌 大阪大学, 工学研究科, 准教授 (80362664)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥47,970,000 (Direct Cost: ¥36,900,000、Indirect Cost: ¥11,070,000); Fiscal Year 2012: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000); Fiscal Year 2011: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000); Fiscal Year 2010: ¥20,670,000 (Direct Cost: ¥15,900,000、Indirect Cost: ¥4,770,000)
• Keywords: 神経保護 / 電気刺激 / 人工視覚 / 網膜機能画像 / ラマン分光 / 網膜機能イメージング

Research Abstract

IGF1 mRNA was increased by the electrical stimulation on cultured RGC5, which suggests that IGF1 is involved in the neuro-protection mechanism by electrical stimulation (ES). The process of neuronal cell-death was demonstrated sequentially and 2-dimensionally by Raman microscope by probing the intracellular changes of cytochrome C. Trans-corneal electrical stimulation (TES) on rabbit model of retinal degeneration showed a decrease of OPL thickness, which suggests the neuro-protective effect of ES on photoreceptors. Clinical study on the artificial vision by optic nerve stimulation showed the efficacy of ES to elicit phosphine for 2 years for advanced retinitis pigmentosa patients.

Research Products

• [Journal Article] Characteristics of Retinal Reflectance Changes Induced by Transcorneal Electrical Stimulation in Cat Eyes (2014)
  • Author(s): Morimoto T, Kanda H, Miyoshi T, Hirohara Y, Mihashi T, Kitaguchi Y, Nishida K, Fujikado T
  • Journal Title: PLOS ONE Volume: 9(3)
• [Journal Article] Characteristics of corneal topographic and pachymetric patterns in patients with pellucid marginal corneal degeneration (2014)
  • Author(s): Fuchihata M, Maeda N, Toda R, Koh S, Fujikado T, Nishida K
  • Journal Title: Jpn J Ophthalmol Volume: 58-2 Issue: 2 Pages: 131-138
  • DOI: 10.1007/s10384-013-0291-3
  • Peer Reviewed
• [Journal Article] Characteristics of Retinal Reflectance Changes Induced by Transcorneal Electrical Stimulation in Cat Eyes. (2014)
  • Author(s): Morimoto Takeshi, Kanda H, Miyoshi T, Hirohara Y, Mihashi T, Kitaguchi Y, Nishida K, Fujikado T
  • Journal Title: PLOS ONE Volume: 9-3 Issue: 3 Pages: 1-11
  • DOI: 10.1371/journal.pone.0092186
  • Peer Reviewed / Open Access
• [Journal Article] Feasibility of 2nd generation STS retinal prosthesis in dogs (2013)
  • Author(s): Fujikado T, Kamei M, Sakaguchi H, Kanda H, Morimoto T, Nishida K, Kishima H, Maruo T, Oosawa K, Ozawa M, Nishida K
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc. Volume: 2013 Pages: 3119-21
• [Journal Article] Feasibility of 2nd generation STS retinal prosthesis in dogs. (2013)
  • Author(s): Fujikado T, Kamei M, Sakaguchi H, Kanda H, Morimoto T, Nishida K, Kishima H, Maruo T, Oosawa K, Ozawa M, Nishida K
  • Journal Title: Conf Proc IEEE Eng Med Biol Soc Volume: Jul Pages: 3119-3121
  • DOI: 10.1109/embc.2013.6610201
  • Peer Reviewed
• [Journal Article] Four discriminant models for detecting keratoconus pattern using Zernike coefficients of corneal aberrations. (2013)
  • Author(s): Saika M, Maeda N, Hirohara Y, Mihashi T, Fujikado T, Nishida K
  • Journal Title: Jpn J Ophthalmol Volume: 57-6 Issue: 6 Pages: 503-509
  • DOI: 10.1007/s10384-013-0269-1
  • Peer Reviewed
• [Journal Article] Optical imaging of retina in response to grating stimuli in cats. (2013)
  • Author(s): Hirohara Y, Mihashi T, Kanda H, Morimoto T, Miyoshi T, Wolffsohn JS, Fujikado T
  • Journal Title: Exp Eye Res Volume: 109C Pages: 1-7
  • DOI: 10.1016/j.exer.2013.01.007
  • Peer Reviewed
• [Journal Article] Transcorneal electrical stimulation promotes survival of photoreceptors and improves retinal function in rhodopsin P347L transgenic rabbits (2012)
  • Author(s): Morimoto T, Kanda H, Kondo M, Terasaki H, Nishida K, Fujikado T
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 28
• [Journal Article] Testing of semichronically implanted retinal prosthesis by suprachoroidal-transretinal stimulation in patients with retinitis pigmentosa (2011)
  • Author(s): Fujikado T, Kamei M, Sakaguchi H, Kanda H, Morimoto T, Ikuno Y, Nishida K, Kishima H, Maruo T, Konoma K, Ozawa M, Nishida K
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 52 Pages: 4726-33
• [Journal Article] Chronic implantation of newly developed suprachoroidal- transretinal stimulation (STS) prosthesis in dogs (2011)
  • Author(s): Morimoto T, Kamei M, Nishida K, Sakaguchi H, Kanda H, Ikuno Y, Kishima H, Maruo T, Konoma K, Ozawa M, Nishida K, Fujikado T
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 52 Pages: 6785-92
• [Journal Article] Testing of semichronically implanted retinal prosthesis by suprachoroidal-transretinal stimulation in patients with retinitis pigmentosa (2011)
  • Author(s): Fujikado T
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 52(7) Issue: 7 Pages: 4726-33
  • DOI: 10.1167/iovs.10-6836
  • Peer Reviewed
• [Journal Article] Chronic implantation of newly developed suprachoroidal-transretinal stimulation (STS) prosthesis in dogs (2011)
  • Author(s): Morimoto T
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 52(9) Issue: 9 Pages: 6785-92
  • DOI: 10.1167/iovs.10-6971
  • Peer Reviewed
• [Journal Article] Biocompatibility and durability of Teflon-coated platinum-iridium wires implanted in the vitreous cavity (2011)
  • Author(s): Nishida K, Sakaguchi H, Xie P, Terasawa Y, Ozawa M, Kamei M, Nishida K
  • Journal Title: J Artif Organs Volume: 14 Issue: 4 Pages: 357-363
  • DOI: 10.1007/s10047-011-0591-7
  • NAID: 10030339473
  • Peer Reviewed
• [Journal Article] Activated Protein C Rescues the Retina from Ischemia-induced Cell Death. (2011)
  • Author(s): Du ZJ, Yamamoto T, Ueda T, Suzuki M, Tano Y, Kamei M
  • Journal Title: Invest Ophthalmol Vis Sci.Epub ahead of print Aug 4.2010 Volume: 52 Pages: 987-993
  • Peer Reviewed
• [Journal Article] Optimal parameters of transcorneal electrical stimulation (TES) to be neuroprotective of axotomized RGCs in adult rats (2010)
  • Author(s): Morimoto T, Miyoshi T, Sawai H, Fujikado T
  • Journal Title: Exp Eye Res. Volume: 90 Pages: 285-91
• [Journal Article] Optimal parameters of transcorneal electrical stimulation (TES) to be neuroprotective of axotomized RGCs in adult rats. (2010)
  • Author(s): Morimoto T, Miyoshi T, Sawai
  • Journal Title: Exp Eye Res. Volume: 90 Pages: 285-291
  • Peer Reviewed
• [Journal Article] Efficacy of suprachoroidal-transretinal stimulation in a rabbit model of retinal degeneration. (2010)
  • Author(s): Nishida K, Kamei M, Kondo M, Sakaguchi H, Suzuki M, Fujikado T, Tano Y.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 51 Pages: 2263-2268
  • Peer Reviewed
• [Journal Article] Development of anisometropia in patients after surgery for esotropia. (2010)
  • Author(s): Fujikado T, Morimoto T, Shimojyo H
  • Journal Title: Jpn J Ophthalmol Volume: 54 Pages: 589-593
  • NAID: 10027666380
  • Peer Reviewed
• [Journal Article] Automated processing of label-free Raman microscope images of macrophage cells with standardized regression for high-throughput analysis (2010)
  • Author(s): Milewski RJ, Kumagai Y, Fujita K, Standley DM, Smith NI.
  • Journal Title: Immunome Res. Volume: 6(open access- on line journal) Pages: 11-11
  • Peer Reviewed
• [Journal Article] Comparison of retinal reflectance changes elicited by transcorneal electrical retinal stimulation to that by optic chiasma stimulation in cats.
  • Author(s): Mihashi T, Okawa Y, Miyoshi T, Kitaguchi Y, Hirohara Y, Fuiikado T
  • Journal Title: Jpn J Ophthalmol Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Clinical application of adaptive optics fundus camera (2014)
  • Author(s): Fujiakdo Takashi
  • Organizer: Japan Taiwan Bilateral Conference on Biomedical and Plasmonic Imaging
  • Place of Presentation: Taipei Taiwan
  • Invited
• [Presentation] Feasibility of 2nd Generation STS Retinal Prosthesis in dogs (2013)
  • Author(s): Fujikado T
  • Organizer: Artificial Vision 2013: The International Symposium on Visual Prosthetics
  • Place of Presentation: Aachen,Germany
  • Year and Date: 2013-11-08
• [Presentation] Evaluation of residual retinal preservation by using transcorneal electrical stimulation and optical coherence tomography in patients with advanced retinitis pigmentosa, candidates for retinal prosthesis (2013)
  • Author(s): Morimoto T
  • Organizer: The International Symposium on Visual Prosthetics
  • Place of Presentation: Aachen,Germany
  • Year and Date: 2013-11-08
• [Presentation] Feasibility of 2nd Generation STS Retinal Prosthesis with 49 Channel Electrode Array in Dogs (2013)
  • Author(s): Fujikado T
  • Organizer: ARVO
  • Place of Presentation: USA, Seattle
  • Year and Date: 2013-05-09
• [Presentation] Feasibility of 2nd Generation STS Retinal Prosthesis in dogs. (2013)
  • Author(s): Fujiakdo T, Kamei M, Sakaguchi H, Kanda H, Morimoto T, Nishida K, Kishima H, Maruo T, Oosawa K, Ozawa M
  • Organizer: Artificial Vision 2013: The International Symposium on Visual Prosthetics
  • Place of Presentation: Aachen,Germany
  • Invited
• [Presentation] Effect of Low‐Additional Soft Contact Lenswith Decentered Optical Design on Myopia Progression inChildren (2013)
  • Author(s): Fujikado Takashi
  • Organizer: Internatinal Myopia Conference
  • Place of Presentation: California, USA
  • Invited
• [Presentation] Feasibility of 2nd Generation STS Retinal Prosthesis in Dogs (2013)
  • Author(s): Fujikado T, Kamei M, Sakaguchi H, Kanda H, Morimoto T, Nishida K, Kishima H, Maruo T, Oosawa K, Ozawa M, Nishida K
  • Organizer: 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society
  • Place of Presentation: Osaka Japan
  • Invited
• [Presentation] Feasibility of 2nd Generation STS Retinal Prosthesis with 49 Channel Electrode Array in Dogs (2013)
  • Author(s): Fujikado T, Kamei M, Kishima H, Maruo T, Osawa K, Ozawa M
  • Organizer: ARVO2013
  • Place of Presentation: Seattle, USA
• [Presentation] Feasibility of Semi-chronically Implanted Retinal Prosthesis by Suprachoroidal-Transretinal Stimulation in Patients with Retinitis Pigmentosa (2011)
  • Author(s): Fujikado T, et al
  • Organizer: Japan-Korea Biomedical Optics Symposium
  • Place of Presentation: Osaka(招待講演)
  • Year and Date: 2011-11-29
• [Presentation] 視神経疾患に対する電気刺激療法 (2011)
  • Author(s): 森本壮
  • Organizer: 第49回日本神経眼科学会総会シンポジウム
  • Place of Presentation: 神戸(招待講演)
  • Year and Date: 2011-11-26
• [Presentation] Feasibility of chronically implanted retinal prosthesis by suprachoroidal-transretinal stimulation in patients with advanced retinitis pigmentosa (2011)
  • Author(s): Morimoto T
  • Organizer: The 2nd KSCEV&JSCEV joint meeting symposium
  • Place of Presentation: 韓国ソウル(招待講演)
  • Year and Date: 2011-11-19
• [Presentation] 重度視覚障害者に対する視覚代行装置(オーデコ)の機能評価 (2011)
  • Author(s): 神田寛行、森本壮、西田幸二、不二門尚
  • Organizer: 第65回日本臨床眼科学会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-09
• [Presentation] 網膜色素変性の残存網膜内層機能の評価-経角膜電気刺激検査と網膜厚の関連の検討 (2011)
  • Author(s): 森本壮、神田寛行、西田幸二、不二門尚
  • Organizer: 第65回日本臨床眼科学会
  • Place of Presentation: 東京
  • Year and Date: 2011-10-07
• [Presentation] 両眼波面センサーによる立体映像視聴時の調節の評価 (2011)
  • Author(s): 神田寛行、松田卓爾、三橋俊文、小林真理子、森本壮、西田幸二、不二門尚
  • Organizer: 第67回日本弱視斜視学会総会
  • Place of Presentation: 京都
  • Year and Date: 2011-07-01
• [Presentation] 次世代の再生医療と手術手技人工網膜埋植の手術手技 (2011)
  • Author(s): 瓶井資弘
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-05-13
• [Presentation] 脈絡膜上-経網膜刺激方式による長期通電の安全性評価 (2011)
  • Author(s): 神田寛行、森本壮、西田健太郎、北口善之、寺澤靖雄、西田幸二、不二門尚
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-12
• [Presentation] ロドプシンP347Lトランスジェニックウサギ眼に対する経角膜電気刺激の神経保護効果 (2011)
  • Author(s): 森本壮、神田寛行、近藤峰生、寺崎浩子、西田幸二、不二門尚
  • Organizer: 第115回日本眼科学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-05-12
• [Presentation] 高次収差と弱視 (2011)
  • Author(s): 森本壮
  • Organizer: 第115回日本眼科学会総会シンポジウム
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2011-05-12
• [Presentation] Evaluation Of The Safety Of Long-term Suprachoroidal-transretinal Stimulation (2011)
  • Author(s): Kanda H, Morimoto T, Nishida K, Kitaguchi Y, Terasawa Y, Nishida K, Fujikado T
  • Organizer: ARVO2011
  • Place of Presentation: 米国フロリダ州フォートローダデール
  • Year and Date: 2011-05-04
• [Presentation] Surgical Approach for Semi-chronic Implantation of Retinal Prosthesis by Suprachoroidal-Transretinal Stimulation (STS) in Patients with Retinitis Pigmentosa (2011)
  • Author(s): Sakaguchi H, Kamei M, Nishida K, Ikuno Y, Morimoto T, Kishima H, Maruo T, Ozawa M, Fujikado T, Nishida K
  • Organizer: ARVO2011
  • Place of Presentation: 米国フロリダ州フォートローダデール
  • Year and Date: 2011-05-04
• [Presentation] Functional Imaging Of The Retina In Response To Grating Light Stimuli (2011)
  • Author(s): Hirohara Y, Mihashi T, Kanda H, Morimoto T, Miyoshi T, Fujikado T
  • Organizer: ARVO2011
  • Place of Presentation: 米国フロリダ州フォートローダデール
  • Year and Date: 2011-05-04
• [Presentation] Feasibility of Semi-chronically Implanted Retinal Prosthesis by Suprach oroidal-Transretinal Stimulation in Patients with Retinitis Pigmentosa (2011)
  • Author(s): Fujikado T, Kamei M, Sakaguchi H, Kanda H, Morimoto T, Ikuno Y, Nishida K, Kishima H, Konoma K, Ozawa M
  • Organizer: ARVO2011
  • Place of Presentation: 米国フロリダ州フォートローダデール
  • Year and Date: 2011-05-03
• [Presentation] Snapshot Spectral Imaging for Functional Imaging of Retina (2011)
  • Author(s): Saika M, Mihashi T, Hirohara Y, Kanda H, Morimoto T, Miyoshi T, Fujikado T
  • Organizer: ARVO2011
  • Place of Presentation: 米国フロリダ州フォートローダデール
  • Year and Date: 2011-05-03
• [Presentation] Transcorneal Electrical Stimulation Promotes The Survival Of Photorece ptors And Preserves Retinal Function In Rhodopsin P3471 Transgenic Rabbits (2011)
  • Author(s): Morimoto T, Kanda H, Kondo M, Terasaki H, Nishida K, Fujikado T
  • Organizer: ARVO2011
  • Place of Presentation: 米国フロリダ州フォートローダデール
  • Year and Date: 2011-05-02
• [Presentation] Identification Of Electrodes In Visual Prosthesis Using Suprachoroidal-Transretinal Stimulation (2011)
  • Author(s): EndoT, Fujikado T, Kamei M, Sakaguchi H, Kanda H, Morimoto T, Kishima H, Konoma K, Ozawa M, Nishida K
  • Organizer: AEVO2011
  • Place of Presentation: 米国フロリダ州フォートローダデール
  • Year and Date: 2011-05-01
• [Presentation] Dynamic Raman observation of cytochromes in apoptotic cells (2011)
  • Author(s): Masaya Okada, Jun Ando, Katsumasa Fujita, Nicholas I.Smith, Satoshi Kawata
  • Organizer: Biomedical Optics, Photonics West 2011, SPIE
  • Place of Presentation: The Moscone center, San Francisco, USA
• [Presentation] Artificial Vision by Direct Optic Nerve Electrode (AV-DONE) (2010)
  • Author(s): Kamei M, Tano Y
  • Organizer: XXIIth MEETING OF THE CLUB JULES GONIN
  • Place of Presentation: 京都(招待講演)
  • Year and Date: 2010-11-04
• [Presentation] Intrinsic optical imaging of retinal response to Transcorneal Electrical Stimulation. (2010)
  • Author(s): Miyoshi T, Kanda H, Morimoto T, Hirohara Y, Mihashi T, Fujikado T
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-09-03
• [Presentation] Transcorneal electrical stimulation, for diagnosis and treatment of retina or optic nerve diseases. (2010)
  • Author(s): Takashi Fujikado
  • Organizer: XXXII International Congress of Ophthalmology
  • Place of Presentation: Berlin・ドイツ
  • Year and Date: 2010-05-06
• [Presentation] A New Electrode Device for Artificial Vision by Direct Optic NerveElectrode (AV-DONE) (2010)
  • Author(s): H.Sakaguchi, K.Nishida, C.Cecilia-Gonzalez, Y.Terasawa, R.Velez-Montoya, T.Fujikado, R.Sanchez-Fontan, M.Ozawa, H.Quiroz-Mercado, M.Kamei.
  • Organizer: ARVO annual meeting 2010
  • Place of Presentation: Fort Lauderdale, アメリカ
• [Book] Role of electrical activity of neurons for neuroprotection (2012)
  • Author(s): Morimoto T
  • Publisher: Int Rev Neurobiol.
• [Book] 【最新の治療デバイス】感覚・運動デバイス人工視覚システム、Clinical Neuroscience 29(4) (2011)
  • Author(s): 神田寛行、不二門尚(分担執筆)
  • Total Pages: 3
  • Publisher: 中外医学社
• [Book] 【加齢黄斑変性最新動向】人工網膜、医学のあゆみ236(13) (2011)
  • Author(s): 神田寛行、不二門尚(分担執筆)
  • Total Pages: 5
  • Publisher: 医歯薬出版株式会社
• [Patent(Industrial Property Rights)] 特許権 (2011)
  • Inventor(s): 瓶井 資弘、坂口 裕和
  • Industrial Property Rights Holder: ㈱ニデック
  • Industrial Property Rights Type: 特許
  • Filing Date: 2011-02-01