Identification of regeneration-associated genes in zebrafish by a ChIP-on-chip assay and its application to mammals.
| Project/Area Number |
22300109
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Kanazawa University |
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Principal Investigator |
KATO Satoru 金沢大学, 医学系, 教授 (10019614)
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| Co-Investigator(Renkei-kenkyūsha) |
TANII Hideji 金沢大学, 医学系, 准教授 (90110618)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 神経再生分子 / HSP70 / エピジェネティクス / HDACインヒビター / 神経生存 / 軸索伸長 / CHIPアッセイ / 転写因子 / NO / HDAC-2 / S-ニトロシル化 / RARβ / 軸索再伸長 / 魚 / 視神経再生 / 細胞生存 / ラット / ゼブラフィッシュ / Hsp70 / 最初期遺伝子 / 網膜神経節細胞 / 細胞生子 |
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| Research Abstract |
Regeneration-associated genes (RAGs) were cloned from axotomized zebrafish retina. HSP70 (a stress protein) and purpurin (a retinol-binding protein) were rapidly induced in the fish retina after optic nerve injury. HSP70 was involved in cell survival of injured retinal ganglion cells (RGCs), whereas purpurin was involved in neurite sprouting from injured RGCs. These RAGs could easily induce regrowth of axotomized optic nerve in adult rat.
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Report
(4 results)
Research Products
(32 results)
