Identification of regeneration-associated genes in zebrafish by a ChIP-on-chip assay and its application to mammals.

• Project/Area Number: 22300109
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Kanazawa University
• Principal Investigator: KATO Satoru 金沢大学, 医学系, 教授 (10019614)
• Co-Investigator(Renkei-kenkyūsha): TANII Hideji 金沢大学, 医学系, 准教授 (90110618)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: 神経再生分子 / HSP70 / エピジェネティクス / HDACインヒビター / 神経生存 / 軸索伸長 / CHIPアッセイ / 転写因子 / NO / HDAC-2 / S-ニトロシル化 / RARβ / 軸索再伸長 / 魚 / 視神経再生 / 細胞生存 / ラット / ゼブラフィッシュ / Hsp70 / 最初期遺伝子 / 網膜神経節細胞 / 細胞生子

Research Abstract

Regeneration-associated genes (RAGs) were cloned from axotomized zebrafish retina. HSP70 (a stress protein) and purpurin (a retinol-binding protein) were rapidly induced in the fish retina after optic nerve injury. HSP70 was involved in cell survival of injured retinal ganglion cells (RGCs), whereas purpurin was involved in neurite sprouting from injured RGCs. These RAGs could easily induce regrowth of axotomized optic nerve in adult rat.

Research Products

• [Journal Article] Neuritogenic activity of trichostatin A in adult rat retinal ganglion cells through acetylation of histone H3 lysine 9 and RAR β induction. (2014)
  • Author(s): Koroyama Y, Sugitani K, Ogai K, Kato S
  • Journal Title: J. Pharmacol. Sci. Volume: 124 Pages: 112-126
  • NAID: 130003382629
  • Peer Reviewed
• [Journal Article] A molecular mechanism of optic nerve regeneration in fish : the retinoid signaling pathway (2013)
  • Author(s): Kato S, Matsukawa T, Koriyama Y, Sugitani K, Ogai K
  • Journal Title: Prog Retin Eye Res. Volume: 37 Pages: 13-30
  • DOI: 10.1016/j.preteyeres.2013.07.004
  • Peer Reviewed
• [Journal Article] Requirement of Retinoic Acid Receptorβfor Genipin Derivative-Induced Optic Nerve Regeneration in Adult Rat Retina (2013)
  • Author(s): Koriyama Y, Takagi Y, Chiba K, Yamazaki M, Sugitani K, Arai K, Suzuki H, Kato S
  • Journal Title: PLoS One Volume: 8 Issue: 8 Pages: e71252-e71252
  • DOI: 10.1371/journal.pone.0071252
  • NAID: 120005327241
  • Peer Reviewed
• [Journal Article] Protective action of nipradilol mediated through S-nitrosylation of Keap1 and HO-1 induction in retinal ganglion cells (2012)
  • Author(s): Koriyama Y
  • Journal Title: Neurochem. Int. Volume: 61 Issue: 7 Pages: 1242-1253
  • DOI: 10.1016/j.neuint.2012.09.004
  • Peer Reviewed
• [Journal Article] Neuritogenic activity of a genipin derivative in retinal ganglion cells is mediated by retinoic acid receptor beta expression through nitric oxide/S-nitrosylation signaling (2011)
  • Author(s): Koriyama Y, Takagi Y, Chiba K, Yamazaki M, Arai K, Matsukawa T, Suzuki H, Sugitani K, Kagechika H, Kato S
  • Journal Title: J. Neurochem. Volume: 119 Issue: 6 Pages: 1232-1242
  • DOI: 10.1111/j.1471-4159.2011.07533.x
  • Peer Reviewed
• [Journal Article] HSP70, the earliest-induced gene in the zebrafish retina during optic nerve regeneration : Its role in cell survival (2011)
  • Author(s): Nagashima M, Fujikawa C, Mawatari K, Mori Y, Kato S
  • Journal Title: Neurochem Int. Volume: 58 Issue: 8 Pages: 888-895
  • DOI: 10.1016/j.neuint.2011.02.017
  • Peer Reviewed
• [Journal Article] HSP70 gene expression in the zebrafish retina after optic nerve injury : A comparative study under heat shock stresses (2011)
  • Author(s): Fujikawa C
  • Journal Title: Adv Exp Med Biol Volume: 723 Pages: 663-668
• [Journal Article] Apoptosis induced by Src-family tyrosine kinase inhibitors in culturedrat cortical cell (2011)
  • Author(s): Takadera T
  • Journal Title: Neurotox Res Volume: 21 Issue: 3 Pages: 309-316
  • DOI: 10.1007/s12640-011-9284-5
  • Peer Reviewed
• [Journal Article] HSP70, the earliest-induced gene in the zebrafish retina during optic nerve regeneration : Its role in cell survival (2011)
  • Author(s): Mikiko Nagashima
  • Journal Title: Neurochemistry International. Volume: (In press)
  • Peer Reviewed
• [Journal Article] 5-S-GAD Attenuates Fe(2+)-induced lipid peroxidation and cell death in a neuronal cell model. (2011)
  • Author(s): Tsuneo Takadera
  • Journal Title: Neurotoxicity Research Volume: (In press)
  • Peer Reviewed
• [Journal Article] Long-acting genipin derivative protects retinal ganglion cells from oxidative stress models in vitro and in vivo through the Nrf2/antioxidant response element signaling pathway (2010)
  • Author(s): Koriyama Y, Chiba K, Yamazaki M, Suzuki H, Muramoto K, Kato S
  • Journal Title: J. Neurochem. Volume: 115 Issue: 1 Pages: 79-91
  • DOI: 10.1111/j.1471-4159.2010.06903.x
  • NAID: 120002561626
  • Peer Reviewed
• [Journal Article] Long-acting genipin derivative protects retinal ganglion cells from oxidative stress models in vitro and in vivo through the Nrf2/ARE signaling pathway. (2010)
  • Author(s): Yoshiki Koriyama
  • Journal Title: Journal of Neurochemistry Volume: 115 Pages: 79-91
  • Peer Reviewed
• [Presentation] ゼブラフィッシュ視神経再生における白血病阻止因子(LIF)の発現増加について (2014)
  • Author(s): 大貝和裕,杉谷加代,郡山恵樹,加藤聖
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学群元キャンパス
  • Year and Date: 2014-03-16
• [Presentation] 酸化的ストレス条件下でのマウス網膜におけるニューログロビンの発現とその機能に関する検討 (2014)
  • Author(s): 杉谷 加代，世良 真悠子，大貝 和裕，郡山 恵樹，若杉 桂輔，加藤 聖
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学群元キャンパス（鹿児島県鹿児島市）
• [Presentation] 'Upregulation of leukemia inhibitory factor (LIF) during zebrafish optic nerve regeneration (2014)
  • Author(s): 大貝 和裕，杉谷 加代，郡山 恵樹，加藤 聖
  • Organizer: 第91回日本生理学会大会
  • Place of Presentation: 鹿児島大学群元キャンパス（鹿児島県鹿児島市）
• [Presentation] A novel function of Factor XIII-A in nerve regeneration (2013)
  • Author(s): Sugitani K
  • Organizer: GTH 2013 (German Trombosis And Heamostasis Society 57th Annual Meeting 2013)
  • Place of Presentation: ICM (International Congress Center Munish)ドイツミュンヘン
• [Presentation] Factor XIII as a regeneration associated molecules in fish retina after optic nerve injury. (2013)
  • Author(s): 杉谷 加代
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
• [Presentation] ゼブラフィッシュ視神経損傷後のleukemia inhibitory factor(LIF)とSox2の発現上昇について (2013)
  • Author(s): 大貝 和裕，杉谷 加代，郡山 恵樹，中島 廣志，加藤 聖
  • Organizer: Neuro2013
  • Place of Presentation: 国立京都国際会館（京都市左京区）
• [Presentation] A novel function of Factor XIII-A in nerve regeneration (2013)
  • Author(s): Sugitani K
  • Organizer: GTH2013
  • Place of Presentation: International Congress Center Munish (ドイツ）
• [Presentation] 中枢神経系におけるKeap1のS-ニトロシル化による抗酸化活性化機構 (2012)
  • Author(s): 郡山恵樹
  • Organizer: 第85回日本薬理学会年会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2012-03-14
• [Presentation] S-nitrosylation of histone deacetylase 2 promotes optic nerve regeneration by retinoic acid receptor beta expression (2012)
  • Author(s): Koriyama Y, Chiba K, Yamazaki M, Sugitani K, Kato S
  • Organizer: RD2012
  • Place of Presentation: Monarch Hotel, Bad Gögging, Germany
• [Presentation] A distinct effect of two types of transglutaminase on fish optic nerve regeneration (2012)
  • Author(s): Sugitani K, Ogai K, Kato S
  • Organizer: The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry, The 55th Annual Meeting of the Japanese Society for Neurochemistry合同大会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] Retinoic acid receptor βinduction by S-nitrosylation of histone deacetylase 2 promotes optic nerve regeneration after injury (2012)
  • Author(s): Koriyama Y, Sugitani K, Chiba K, Karo S
  • Organizer: The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry, The 55th Annual Meeting of the Japanese Society for Neurochemistry合同大会
  • Place of Presentation: 神戸国際会議場（兵庫県）
• [Presentation] S-nitrosylation of histone deacetylase 2 promotes optic nerve regeneration by retinoic acid receptor beta expression (2012)
  • Author(s): Koriyama Y, Chiba K, Yamazaki M, Sugitani K, Kato S
  • Organizer: RD2012
  • Place of Presentation: Monarch Hotel (ドイツ）
• [Presentation] Reciprocal changes of factor XIII and retinal transglutaminase expressions in the fish retina during optic nerve regeneration (2012)
  • Author(s): Sugitani K, Ogai K, Kato S
  • Organizer: RD2012
  • Place of Presentation: Monarch Hotel (ドイツ）
• [Presentation] 視神経損傷後の新規細胞外マトリックス分子プルプリンによるラット網膜神経節細胞の生存と軸索伸長 (2011)
  • Author(s): Koriyama Y, Matsukawa T, Nagashima M, Kato S
  • Organizer: 第54回日本神経化学会大会
  • Place of Presentation: 石川県加賀市山代温泉「瑠璃光」
  • Year and Date: 2011-09-28
• [Presentation] 視神経損傷後の新規細胞外マトリックス分子プルプリンによるラット網膜神経節細胞の生存と軸索伸長 (2011)
  • Author(s): 郡山恵樹
  • Organizer: 第54回日本神経化学会
  • Place of Presentation: 瑠璃光(石川)
  • Year and Date: 2011-09-28
• [Presentation] The expression of KLF4 in zebrafish retina during optic nerve regeneration (2010)
  • Author(s): Maki Nishitani
  • Organizer: Neuro2010
  • Place of Presentation: 神戸コンベンションセンター(兵庫県)
• [Presentation] The earliest inducible molecule in the zebrafish retina for optic nerve regeneration. (2010)
  • Author(s): Chieko Fujikawa
  • Organizer: RD2010
  • Place of Presentation: Fairmont Tremblant Hotel(カナダ)
• [Presentation] Factor XIIIA induction of regenerating retina and axons in the fish ocular system. (2010)
  • Author(s): Kayo Sugitani
  • Organizer: RD2010
  • Place of Presentation: Fairmont Tremblant Hotel(カナダ)
• [Remarks]
  • URL: http://neuro.w3.kanazawa-u.ac.jp
• [Remarks] 金沢大学大学院医学系研究科脳医科学専攻脳細胞分子学講座脳情報分子学
  • URL: http://neuro.w3.kanazawa-u.ac.jp/