Study of the molecular mechanisms of synaptic plasticity and itsinvolvements in human diseases.

• Project/Area Number: 22300122
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: University of Miyazaki
• Principal Investigator: TAKAMIYA Kogo 宮崎大学, 医学部, 教授 (40283767)
• Co-Investigator(Renkei-kenkyūsha): WAKAZONO Yoshihiko 宮崎大学, 医学部, 助教 (90377755)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2010: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
• Keywords: シナプス可塑性 / グルタミン酸受容体 / 学習・記憶

Research Abstract

We studied the molecular mechanisms of synaptic plasticity in this project. Inparticular, we focused on the GRIP protein on AMPA receptor trafficking, which plays a key role insynaptic plasticity expression. It turned out that GRIP is located in exocyst and play an importantfunction in AMPA receptor cell surface expression. In addition, we have established a system toaccurately introduce exogenous gene into hippocampus to study synaptic plasticity in vivo.

Research Products

• [Journal Article] Involvement of AMPA Receptor GluR2 and GluR3Trafficking in Trigeminal Spinal Subnucleus Caudalis andC1/C2 Neurons in Acute-Facial Inflammatory Pain. (2012)
  • Author(s): Miyamoto M, Tsuboi Y, Honda K, Kobayashi M,Takamiya K, Huganir RL, Kondo M, Shinoda M, SessleBJ, Katagiri A, Kita D, Suzuki I, Oi Y, Iwata K.
  • Journal Title: PLoS One
  • Peer Reviewed
• [Journal Article] Involvement of AMPA Receptor GluR2 and GluR3 Trafficking in Trigeminal Spinal Subnucleus Caudalis and C1/C2 Neurons in Acute-Facial Inflammatory Pain. (2012)
  • Author(s): Miyamoto, M.
  • Journal Title: PLoS One Volume: 7 Issue: 8 Pages: e44055-e44055
  • DOI: 10.1371/journal.pone.0044055
  • Peer Reviewed / Open Access
• [Journal Article] GluA3-deficiency in mice is associated with increased social and aggressive behavior and elevated dopamine in striatum. (2012)
  • Author(s): Adamczyk, A.
  • Journal Title: Behavioural brain research Volume: 229 Issue: 1 Pages: 265-272
  • DOI: 10.1016/j.bbr.2012.01.007
  • Peer Reviewed
• [Journal Article] DlgS97/SAP97, a neuronal isoform of discs large, regulates ethanol tolerance. (2012)
  • Author(s): Maiya, R.
  • Journal Title: PLoS One Volume: 7 Issue: 11 Pages: e48967-e48967
  • DOI: 10.1371/journal.pone.0048967
  • Peer Reviewed
• [Journal Article] Characterization of in vivo dlg1 deletion on T cell development and function (2012)
  • Author(s): Humphries, L. A.
  • Journal Title: PLoS One Volume: 7 Issue: 9 Pages: e45276-e45276
  • DOI: 10.1371/journal.pone.0045276
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of synaptic plasticity underlyinglearning and memory (2011)
  • Author(s): Takamiya K.
  • Journal Title: Seikagaku Pages: 1016-1026
• [Journal Article] Involvement of GluR2 and GluR3 subunit C-termini inthe trigeminal spinal subnucleus caudalis and C1-C2neurons in trigeminal neuropathic pain. (2011)
  • Author(s): Miyamoto M,Tsuboi Y, Takamiya K, Huganir RL, Kondo M, Shinoda M,Oi Y, Iwata K.
  • Journal Title: Neuroscience letters Pages: 8-12
  • Peer Reviewed
• [Journal Article] Enhanced synaptic plasticity in mice withphosphomimetic mutation of the GluA1 AMPA receptor. (2011)
  • Author(s): Makino Y, Johnson RC, Yu Y, Takamiya K, Huganir RL.
  • Journal Title: Proc Natl Acad Sci U S A Pages: 8450-8455
  • Peer Reviewed
• [Journal Article] Phosphorylation of AMPA receptors is required forsensory deprivation-induced homeostatic synapticplasticity. (2011)
  • Author(s): Goel A, Xu LW, Snyder KP, Song L,Goenaga-Vazquez Y, Megill A, Takamiya K, Huganir RL,Lee HK.
  • Journal Title: PLoS One
  • Peer Reviewed
• [Journal Article] GluA4 is Indispensable for Driving Fast Neurotransmission across a High-fidelity Central Synapse. J Physiol (2011)
  • Author(s): Yang, Y.M., Aitoubah, J., Lauer, A.M., Nuriya, M., Takamiya, K., Jia, Z., May, B.J., Huganir, R.L., Wang, L.Y.
  • Journal Title: J Physiol Volume: 589 Issue: 17 Pages: 4209-27
  • DOI: 10.1113/jphysiol.2011.208066
  • Peer Reviewed
• [Journal Article] Preserved acute pain and impaired neuropathic pain in mice lacking protein interacting with C Kinase 1 (2011)
  • Author(s): Wang, W., Petralia, R.S., Takamiya, K., Xia, J., Li, Y.Q., Huganir, R.L., Tao, Y.X., Yaster, M
  • Journal Title: Molecular pain Volume: 7 Pages: 11-11
  • DOI: 10.1186/1744-8069-7-11
  • Peer Reviewed
• [Journal Article] Reevaluating the Role of LTD in Cerebellar Motor Learning (2011)
  • Author(s): Schonewille, M., Gao, Z., Boele, H.J., Vinueza Veloz, M.F., Amerika, W.E., Simek, A.A., De Jeu, M.T., Steinberg, J.P., Takamiya, K., Hoebeek, F.E., et al
  • Journal Title: Neuron Volume: 71 Issue: 1 Pages: 43-50
  • DOI: 10.1016/j.neuron.2011.02.044
  • Peer Reviewed
• [Journal Article] Regulation of AMPA receptor function by the human memory-associated gene KIBRA (2011)
  • Author(s): Makuch, L., Volk, L., Anggono, V., Johnson, R.C., Yu, Y., Duning, K, Kremerskothen, J., Xia, J., Takamiya, K, Huganir, R.L.
  • Journal Title: Neuron Volume: 71 Issue: 6 Pages: 1022-1029
  • DOI: 10.1016/j.neuron.2011.08.017
  • Peer Reviewed
• [Journal Article] Enhanced synaptic plasticity in mice with phosphomimetic mutation of the GluA1 AMPA receptor (2011)
  • Author(s): Makino, Y., Johnson, R.C., Yu, Y., Takamiya, K., Huganir, R.L.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108 Issue: 20 Pages: 8450-8455
  • DOI: 10.1073/pnas.1105261108
  • Peer Reviewed
• [Journal Article] Phosphorylation of AMPA receptors is required for sensory deprivation-induced homeostatic synaptic plasticity (2011)
  • Author(s): Goel, A., Xu, L.W., Snyder, K.P., Song, L., Goenaga-Vazquez, Y., Megill, A., Takamiya, K., Huganir, R.L., Lee, H.K.
  • Journal Title: PLoS One Volume: 6 Issue: 3 Pages: 1016-1026
  • DOI: 10.1371/journal.pone.0018264
  • Peer Reviewed
• [Journal Article] Developmental regulation of protein interacting with C kinase 1(PICK1)function in hippocampal synaptic plasticity and learning. (2011)
  • Author(s): Volk L, Kim CH, Takamiya K, Yu Y, Huganir RL
  • Journal Title: Proc Natl Acad Sci U S A
  • Peer Reviewed
• [Journal Article] Spinal cord protein interacting with C kinase 1 is required for the maintenance of complete Freund's adjuvant-induced inflammatory pain but not for incision-induced post-operative pain. (2011)
  • Author(s): Atianjoh FE, Yaster M, Zhao X, Takamiya K, Xia J, Gauda EB, Huganir RL, Tao YX
  • Journal Title: Pain Volume: 151 Pages: 226-234
  • Peer Reviewed
• [Journal Article] Involvement of GluR2 and GluR3 subunit C-termini in the trigeminal spinal subnucleus caudalis and C1-C2 neurons in trigeminal neuropathic pain (2011)
  • Author(s): Miyamoto M, Tsuboi Y, Takamiya K, Huganir RL, Kondo M, Shinoda M, Oi Y, Iwata K
  • Journal Title: Neurosci Lett Volume: 491 Pages: 8-12
  • Peer Reviewed
• [Journal Article] 高次脳神経機能、特に学習・記憶におけるシナプス可塑性の分子機構(総説) (2011)
  • Author(s): 高宮考悟
  • Journal Title: 宮崎県医師会医学会誌 Volume: 34 Pages: 1-10
• [Journal Article] Developmental regulation of protein interacting with Ckinase 1 (PICK1) function in hippocampal synapticplasticity and learning. (2010)
  • Author(s): Volk L, Kim CH, Takamiya K, YuY, Huganir RL.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 21784-21789
  • Peer Reviewed
• [Journal Article] GRIP1 and 2 regulate activity-dependent AMPA receptorrecycling via exocyst complex interactions. (2010)
  • Author(s): Mao L,Takamiya K, Thomas G, Lin DT, Huganir RL.
  • Journal Title: Proceedings of the National Academy of Sciences of theUnited States of America Volume: 19038-19043
  • Peer Reviewed
• [Journal Article] Specific roles of AMPA receptor subunit GluR1(GluA1)phosphorylation sites in regulating synaptic plasticity in the CA1 region of hippocampus. (2010)
  • Author(s): Lee HK, Takamiya K, He K, Song L, Huganir RL
  • Journal Title: J Neurophysiol Volume: 103 Pages: 479-489
  • Peer Reviewed
• [Journal Article] GRIP1 and 2 regulate activity-dependent AMPA receptor recycling via exocyst complex interactions. (2010)
  • Author(s): Mao L, Takamiya K, Thomas G, Lin DT, Huganir RL
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Pages: 19038-19043
  • Peer Reviewed
• [Presentation] Organic and inorganic arsenic impairsynaptic potentials: Studies on GluA1trafficking. (2012)
  • Author(s): H. Madhyastha, M. Maruyama, K. Takamiya
  • Organizer: 北米神経科学界
• [Presentation] LTP誘導機構解明のための海馬CA1領域選択的、外来遺伝子導入法の確立とその適用 (2012)
  • Author(s): 髙宮考悟
  • Organizer: 第３５回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 糖鎖シグナルによるシナプス可塑性の制御機構 (2012)
  • Author(s): 髙宮考悟
  • Organizer: 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 仙台
• [Presentation] AMPA型グルタミン酸受容体のN型糖鎖修飾による電流特性への影響 (2012)
  • Author(s): 髙宮考悟
  • Organizer: 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 仙台
• [Presentation] 脂質ラフトにおけるAMPA型グルタミン酸受容体GluA1の輸送制御 (2012)
  • Author(s): 髙宮考悟
  • Organizer: 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 仙台
• [Presentation] 学習・記憶におけるシナプス可塑性の分子メカニズム AMPA型グルタミン酸レセプターによる神経機能の制御 (2012)
  • Author(s): 高宮考悟
  • Organizer: 生理学研究所研究会 シナプス可塑性の動作原理 分子から行動まで
  • Place of Presentation: 岡崎
• [Presentation] 学習・記憶におけるシナプス可塑性の分子メカニズム-AMPA型グルタミン酸レセプターによる神経機能の制御- (2011)
  • Author(s): 高宮考悟
  • Organizer: 第5回トランスポーター研究会
  • Place of Presentation: 宮崎(招待講演)
  • Year and Date: 2011-09-22
• [Presentation] The selective injection system utilizing theta oscillation (2011)
  • Author(s): 高宮考悟
  • Organizer: 神経科学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] 学習・記憶におけるシナプス可塑性の分子メカニズム-AMPA型グルタミン酸レセプターによる神経機能の制御- (2011)
  • Author(s): 高宮考悟
  • Organizer: 第15回神経科学領域における分子モニタリングシンポジウム
  • Place of Presentation: 宮崎
  • Year and Date: 2011-06-13
• [Presentation] LTP 誘導機構解明のための海馬 CA1 領域選択的、外来遺伝子導入法の確立とその適用Application of hippocampus CA1-selectivegene transfer system for analyzing LTPexpression mechanisms
  • Author(s): Y. Wakazono, J. Tsutajima, T. Kunitake, K.Takamiya
  • Organizer: 第35回日本神経科学大会
• [Remarks]
  • URL: http://www.med.miyazaki-u.ac.jp/1physiol/default.html
• [Remarks]
  • URL: http://www.med.miyazaki-u.ac.jp/lphysiol/default.html