Roles of maturation of presynaptic functions in the postnatal refinement of neuronal circuits.

• Project/Area Number: 22300125
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Hiroshima University
• Principal Investigator: HASHIMOTO Kouichi 広島大学, 医歯薬保健学研究院, 教授 (00303272)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2010: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: ニューロン / シナプス / 神経回路 / 小脳 / 生後発達

Research Abstract

Postnatal changes of electrophysiological properties of inferior olivery (IO) neurons are examined by the patch clamp recording using slices prepared from mouse medulla. The threshold of action potential was increasedand input resistance was decreased from postnatal day 7 (P7) to P15. These developmental changes proceeded coincident with the formation of the gap junction. Moreover, the subthreshold oscillation also started during this postnatal period. These results suggestthat IO neurons gain their adult type membrane properties from P7 to P15.

Research Products

• [Journal Article] Organotypic Coculture Preparation for the Study of Developmental Synapse Elimination in Mammalian Brain (2012)
  • Author(s): Uesaka, N., Mikuni, T., Hashimoto, K., Hirai, H., Sakimura, K. & Kano, M
  • Journal Title: J Neurosci Volume: 32 Pages: 11657-11670
  • Peer Reviewed
• [Journal Article] Synapse type-independent degradation of the endocannabinoid 2-arachidonoylglycerol after retrograde synaptic suppression. (2012)
  • Author(s): Tanimura, A., Uchigashima, M., Yamazaki, M., Uesaka, N., Mikuni, T., Abe, M., Hashimoto, K., Watanabe, M., Sakimura, K. & Kano, M
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 109 Pages: 12195-12200
  • Peer Reviewed
• [Journal Article] GABAergic inhibition regulates developmental synapse elimination in the cerebellum (2012)
  • Author(s): Nakayama, H., Miyazaki, T., Kitamura, K., Hashimoto, K., Yanagawa, Y., Obata, K., Sakimura, K., Watanabe, M. & Kano, M
  • Journal Title: Neuron Volume: 74 Pages: 384-396
  • Peer Reviewed
• [Journal Article] Cav2.1 in cerebellar Purkinje cells regulates competitive excitatory synaptic wiring, cell survival, and cerebellar biochemical compartmentalization (2012)
  • Author(s): Miyazaki, T., Yamasaki, M., Hashimoto, K., Yamazaki, M., Abe, M., Usui, H., Kano, M., Sakimura, K. & Watanabe, M
  • Journal Title: J. Neurosci Volume: 32 Pages: 1311-1328
  • NAID: 120005208889
  • Peer Reviewed
• [Journal Article] Activity-Dependent Maturation of Climbing Fiber to Purkinje Cell Synapses during Postnatal Cerebellar Development (2012)
  • Author(s): Kano, M., Hashimoto, K
  • Journal Title: Cerebellum Volume: 11 Pages: 449-450
  • Peer Reviewed
• [Journal Article] Organotypic coculture preparation for the study of developmental synapse elimination in mammalian brain (2012)
  • Author(s): Uesaka N
  • Journal Title: J Neurosci Volume: 32 Issue: 34 Pages: 11657
  • DOI: 10.1523/jneurosci.1097-12.2012
  • Peer Reviewed
• [Journal Article] Synapse type-independent degradation of the endocannabinoid 2-arachidonoylglycerol after retrograde synaptic suppression (2012)
  • Author(s): Tanimura A
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Issue: 30 Pages: 12195
  • DOI: 10.1073/pnas.1204404109
  • Peer Reviewed
• [Journal Article] Activity-dependent maturation of climbing fiber to purkinje cell synapses during postnatal cerebellar development (2012)
  • Author(s): Kano M
  • Journal Title: Cerebellum Volume: 11 Issue: 2 Pages: 449
  • DOI: 10.1007/s12311-011-0337-3
  • Peer Reviewed
• [Journal Article] GABAergic Inhibition Regulates Developmental Synapse Elimination in the Cerebellum (2012)
  • Author(s): Nakayama H
  • Journal Title: Neuron Volume: 74 Issue: 2 Pages: 384
  • DOI: 10.1016/j.neuron.2012.02.032
  • Peer Reviewed
• [Journal Article] Ca _v2.1 in cerebellar Purkinje cells regulates competitive excitatory synaptic wiring, cell survival, and cerebellar biochemical compartmentalization (2012)
  • Author(s): Miyazaki, Taisuke ; Yamasaki, Miwako ; Hashimoto, Kouichi, et
  • Journal Title: Journal of Neuroscience Volume: 32 Issue: 4 Pages: 1311
  • DOI: 10.1523/jneurosci.2755-11.2012
  • NAID: 120005208889
  • Peer Reviewed
• [Journal Article] Developmental switching of perisomatic innervation from climbing fibers to Basket cell fibers in cerebellar Purkinje cells (2011)
  • Author(s): Ichikawa, R., Yamasaki, M., Miyazaki, T., Konno, K., Hashimoto, K., Tatsumi, H., Inoue, Y., Kano, M. & Watanabe, M
  • Journal Title: J Neurosci Volume: 31 Pages: 16916-16927
  • NAID: 120005183171
  • Peer Reviewed
• [Journal Article] Postsynaptic P/Q-type Ca2+ channels in cerebellar Purkinje cells mediate synaptic competition among multiple climbing fiber inputs during postnatal development. (2011)
  • Author(s): Hashimoto, K., Tsujita, M., Miyazaki, T., Kitamura, K., Yamazaki, M., Shin, H.S., Watanabe, M., Sakimura, K. & Kano, M
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 108 Pages: 9987-9992
  • Peer Reviewed
• [Journal Article] A Role for Myosin Va in Cerebellar Plasticity and Motor Learning: A Possible Mechanism Underlying Neurological Disorder in Myosin Va Disease (2011)
  • Author(s): Miyata, M., Kishimoto, Y., Tanaka, M., Hashimoto, K., Hirashima, N., Murata, Y., Kano, M. & Takagishi, Y
  • Journal Title: J Neurosci. Volume: 31 Pages: 6067-6078
  • Peer Reviewed
• [Journal Article] Postsynaptic P/Q-type Ca²⁺ channel in Purkinje cell mediates synaptic competition and elimination in developing cerebellum (2011)
  • Author(s): Hashimoto, K.; Tsujita, M.; Miyazaki, T., et al
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 Issue: 24 Pages: 9987
  • DOI: 10.1073/pnas.1101488108
  • Peer Reviewed
• [Journal Article] Developmental switching of perisomatic innervation from climbing fibers to basket cell fibers in cerebellar Purkinje cells (2011)
  • Author(s): Ichikawa, R.; Yamasaki, M.; Miyazaki, T., et al
  • Journal Title: Journal of Neuroscience Volume: 31 Issue: 47 Pages: 16916
  • DOI: 10.1523/jneurosci.2396-11.2011
  • NAID: 120005183171
  • Peer Reviewed
• [Journal Article] A role for myosin Va in cerebellar plasticity and motor learning: A possible mechanism underlying neurological disorder in myosin Va disease (2011)
  • Author(s): Miyata M, Kishimoto Y, Tanaka M, Hashimoto K, Hirashima N, Murata Y, Kano M, Takagishi Y
  • Journal Title: J.Neurosci. Volume: 31 Issue: 16 Pages: 6067
  • DOI: 10.1523/jneurosci.5651-10.2011
  • Peer Reviewed
• [Journal Article] A role for myosin Va in cerebellar plasticity and motor learning : a possible mechanism underlying neurological disorder in myosin Va disease. (2011)
  • Author(s): Miyata M, Kishimoto Y, Tanaka M, Hashimoto K, Hirashima N, Murata Y, Kano M, Takagishi Y
  • Journal Title: Journal of Neuroscience Volume: (In press)
  • Peer Reviewed
• [Journal Article] TARPs γ-2 and γ-7 are essential for AMPA receptor expression in the cerebellum. (2010)
  • Author(s): Yamazaki, M., Fukaya, M., Hashimoto, K., Yamasaki, M., Tsujita, M., Itakura, M., Abe, M., Natsume, R., Takahashi, M., Kano, M., Sakimura, K. & Watanabe, M
  • Journal Title: Eur. J. Neurosci Volume: 31 Pages: 2204-2220
  • Peer Reviewed
• [Journal Article] TARPs gamma-2 and gamma-7 are essential for AMPA receptor expression in the cerebellum. (2010)
  • Author(s): Yamazaki M, Fukaya M, Hashimoto K, Yamasaki M, Tsujita M, Itakura M, Abe M, Natsume R, Takahashi M, Kano M, Sakimura K, Watanabe M
  • Journal Title: European Journal of Neuroscience Volume: 31 Pages: 2204-2220
  • Peer Reviewed
• [Presentation] Postnatal refinement of the cerebellar climbing fiber to Purkinje cell synapse (2013)
  • Author(s): K. Hashimoto
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] 小脳登上線維-プルキンエ細胞シナプスの発達において競合する登上線維間の相対的な強度の差と登上線維の絶対的な強度がそれぞれ異なる段階に寄与している (2013)
  • Author(s): 川田 慎也、橋本 浩一、山崎 真弥、宮崎 太輔、山崎美和子、三國貴康、渡辺 雅彦、崎村 健司、狩野 方伸
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京
• [Presentation] Postnatal refinement of the cerebellar climbing fiber to Purkinje cell synapse (2013)
  • Author(s): Hashimoto K
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京 日本
• [Presentation] 小脳登上線維-プルキンエ細胞シナプスの発達において競合する登上線維間の相対的な強度の差と登上線維の絶対的な強度がそれぞれ異なる段階に寄与している (2013)
  • Author(s): 川田慎也
  • Organizer: 第90回日本生理学会大会
  • Place of Presentation: 東京 日本
• [Presentation] Synapse type-independent degradation of 2-arachidonoylglycerol after retrograde synaptic suppression in the cerebellum. (2012)
  • Author(s): Tanimura A., Uchigashima M., Yamazaki M., Uesaka N., Mikuni T., Hashimoto K., Watanabe M., Sakimura K., Kano M.
  • Organizer: Annual meetnig of Society for Neuroscience
  • Place of Presentation: New Orleans、USA
• [Presentation] 小脳プルキンエ細胞特異的γ2 ノックアウトマウスにおける登上線維の刈り込み及び樹状突起への伸展の異常 (2012)
  • Author(s): 川田慎也、橋本浩一、山崎真弥、宮崎太輔、渡辺雅彦、崎村健司、狩野方伸
  • Organizer: 第35回日本神経科学学会大会
  • Place of Presentation: 名古屋
• [Presentation] 小脳プルキンエ細胞特異的γ2ノックアウトマウスにおける登上線維の刈り込み及び樹状突起への伸展の異常 (2012)
  • Author(s): 川田慎也
  • Organizer: 第35回日本神経科学学会大会
  • Place of Presentation: 愛知 日本
• [Presentation] Synapse type-independent degradation of 2-arachidonoylglycerol after retrograde synaptic suppression in the cerebellum (2012)
  • Author(s): Tanimura A
  • Organizer: Annual meetnig of Society for Neuroscience
  • Place of Presentation: New Orleans USA
• [Presentation] 発達期小脳登上線維-プルキンエ細胞間シナプス除去への抑制性シナプス伝達の関与 (2011)
  • Author(s): 中山寿子、宮崎太輔、橋本浩一、柳川右千夫、小幡邦彦、崎村建司、渡辺雅彦、狩野方伸
  • Organizer: 第63回日本生理学会中国四国地方会
  • Place of Presentation: 広島
  • Year and Date: 2011-10-23
• [Presentation] 登上線維の生後発達における P/Q 型電位依存性カルシウムチャネルの役割 (2011)
  • Author(s): 橋本浩一、辻田実加、宮崎太輔、喜多村和郎、山崎真弥、Shin Hee-sup、渡辺雅彦、崎村建司、狩野方伸
  • Organizer: 第34回日本神経科学学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] 登上線維の生後発達におけるP/Q型電位依存性カルシウムチャネルの役割 (2011)
  • Author(s): 橋本浩一
  • Organizer: 第34回日本神経科学学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-17
• [Presentation] 2-アラキドノイルグリセロールリパーゼは小脳皮質においてシナプス非特異的に分解される (2011)
  • Author(s): 谷村あさみ、山崎真弥、内が島基政、上阪直史、三国貴康、橋本浩一、渡辺雅彦、崎村建司、狩野方伸
  • Organizer: 第34回日本神経科学学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2011-09-15
• [Presentation] 生後発達期小脳における神経回路の再編成 (2011)
  • Author(s): 橋本浩一
  • Organizer: 「包括型脳科学研究推進支援ネットワーク」夏のワークショップ
  • Place of Presentation: 神戸
  • Year and Date: 2011-08-22
• [Presentation] プルキンエ細胞選択的Cav2.1(P/Q型電位依存性カルシウムチャネル)ノックアウトマウスにおける登上線維-プルキンエ細胞シナプスの生後発達過程の異常 (2011)
  • Author(s): 橋本浩一
  • Organizer: 「包括型脳科学研究推進支援ネットワーク」夏のワークショップ
  • Place of Presentation: 神戸
  • Year and Date: 2011-08-22
• [Presentation] プルキンエ細胞選択的Cav2.1(P/Q 型電位依存性カルシウムチャネル)ノックアウトマウスにおける登上線維-プルキンエ細胞シナプスの生後発達過程の異常 (2011)
  • Author(s): 橋本浩一
  • Organizer: 包括型脳科学研究推進支援ネットワーク 夏のワークショップ
  • Place of Presentation: 神戸
• [Presentation] 橋本浩一 (2011)
  • Author(s): 橋本浩一
  • Organizer: 包括型脳科学研究推進支援ネットワーク 夏のワークショップ
  • Place of Presentation: 神戸
• [Presentation] 小脳登上線維-プルキンエ細胞シナプスの生後発達における P/Q 型電位依存性カルシウムチャネルの役割 (2010)
  • Author(s): 橋本 浩一、辻田 実加、宮崎 太輔、山崎 真弥、喜多村 和郎、Hee-Sup Shin、渡辺 雅彦、崎村 建司、狩野 方伸
  • Organizer: 第87回日本生理学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-05-21
• [Presentation] 登上線維の発達過程における P/Q 型電位依存性カルシウムチャネルの役割 (2010)
  • Author(s): 橋本 浩一、辻田 実加、喜多村 和郎、宮崎 太輔、山崎 真弥、Hee-Sup Shin、渡辺 雅彦、崎村 建司、狩野 方伸
  • Organizer: 第33回日本神経科学大会、第53回日本神経化学会大会および第20回日本神経回路学会大会
  • Place of Presentation: 神戸
• [Presentation] TARPs γ -2 and γ -7 are functional components of cerebellar AMPA receptor. (2010)
  • Author(s): Yamazaki M, Fukaya M, Hashimoto K, Yamasaki M, Itakura M, Takahashi M, Kano M, Watanabe M, Sakimura K
  • Organizer: 第33回日本神経科学大会、第53回日本神経化学会大会および第20回日本神経回路学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] Analysis of physiological functions of cerebellar AMPA receptor subunits using a Purkinje cell-selective gene targeting system. (2010)
  • Author(s): Abe M, Hashimoto K, Miyazaki T, Azechi H, Natsume R, Watanabe M, Kano M, Sakimura K
  • Organizer: 第33回日本神経科学大会、第53回日本神経化学会大会および第20回日本神経回路学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] 2-Arachidonoylglycerol produced by diacylglycerol lipase α mediates retrograde suppression of synaptic transmission in the CNS. (2010)
  • Author(s): Tanimura A, Yamazaki M, Hashimotodani Y, Uchigashima M, Kawata S, Abe M, Kita Y, Hashimoto K, Shimizu T, Watanabe M, Sakimura K, Kano M
  • Organizer: 第33回日本神経科学大会、第53回日本神経化学会大会および第20回日本神経回路学会大会 合同大会
  • Place of Presentation: 神戸
• [Presentation] Transmembrane AMPA receptor regulatory protein gamma-2 and gamma-7 are essential for cerebellar AMPA receptor complex (2010)
  • Author(s): Yamazaki M, Fukaya M, Hashimoto K, Kano M, Watanabe M, Sakimura K
  • Organizer: 7th FENS forum of European Neuroscience
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] Retrograde synaptic suppression in the cerebellum, hippocampus and striatum is mediated by 2-arachidonoylglycerol produced by diacylglycerol lipase alpha (2010)
  • Author(s): Tanimura A, Yamazaki M, Hashimotodani Y, Uchigashima M, Kawata S, Abe M, Kita Y, Hashimoto K, Shimizu T, Watanabe M, Sakimura K, Kano M
  • Organizer: 7th FENS forum of European Neuroscience
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] 小脳登上線維-プルキンエ細胞シナプスの生後発達におけるP/Q型電位依存性カルシウムチャネルの役割 (2010)
  • Author(s): 橋本浩一、辻田実加、宮崎太輔、山崎真弥、喜多村和郎、Hee-SupShin、渡辺雅彦、崎村建司、狩野方伸
  • Organizer: 第87回 日本生理学会大会
  • Place of Presentation: 盛岡市民文化ホール
• [Presentation] P/Q type voltage dependent Ca2+ channel is crucial for early postnatal development of climbing fiber to Purkinje cell synapse (2010)
  • Author(s): Hashimoto K, Tsujita M, Kitamura K, Miyazaki T, Yamazaki M, Shin HS, Watanabe M, Sakimura K, Kano M
  • Organizer: 第33回日本神経科学大会、第53回日本神経化学会大会および第20回日本神経回路学会大会 合同大会
  • Place of Presentation: 神戸コンベンションセンター(招待講演)
• [Presentation] TARPs γ-2 and γ-7 are functional components of cerebellar AMPA receptor (2010)
  • Author(s): Yamazaki M, Fukaya M, Hashimoto K, Yamasaki M, Itakura M, Takahashi M, Kano M, Watanabe M, Sakimura K
  • Organizer: 第33回日本神経科学大会、第53回日本神経化学会大会および第20回日本神経回路学会大会 合同大会
  • Place of Presentation: 神戸コンベンションセンター
• [Presentation] Analysis of physiological functions of cerebellar AMPA receptor subunits using a Purkinje cell-selective gene targeting system (2010)
  • Author(s): Abe M, Hashimoto K, Miyazaki T, Azechi H, Natsume R, Watanabe M, Kano M, Sakimura K
  • Organizer: 第33回日本神経科学大会、第53回日本神経化学会大会および第20回日本神経回路学会大会 合同大会
  • Place of Presentation: 神戸コンベンションセンター
• [Presentation] 2-Arachidonoylglycerol produced by diacylglycerol lipase α mediates retrograde suppression of synaptic transmission in the CNS. (2010)
  • Author(s): Tanimura A, Yamazaki M, Hashimotodani Y, Uchigashima M, Kawata S, Abe M, Kita Y, Hashimoto K, Shimizu T, Watanabe M, Sakimura K, Kano M
  • Organizer: 第33回日本神経科学大会、第53回日本神経化学会大会および第20回日本神経回路学会大会 合同大会
  • Place of Presentation: 神戸コンベンションセンター
• [Presentation] Transmembrane AMPA receptor regulatory protein gamma-2 and gamma-7 are essential for cerebellar AMPA receptor complex (2010)
  • Author(s): Yamazaki M, Fukaya M, Hashimoto K, Kano M, Watanabe M, Sakimura K
  • Organizer: 7^<th> FENS forum of European Neuroscience
  • Place of Presentation: Amsterdam, Netherlands
• [Presentation] Retrograde synaptic suppression in the cerebellum, hippocampus and striatum is mediated by 2-arachidonoylglycerol produced by diacylglycerol lipase alpha (2010)
  • Author(s): Tanimura A, Yamazaki M, Hashimotodani Y, Uchigashima M, Kawata S, Abe M, Kita Y, Hashimoto K, Shimizu T, Watanabe M, Sakimura K, Kano M
  • Organizer: 7^<th> FENS forum of European Neuroscience
  • Place of Presentation: Amsterdam, Netherlands
• [Remarks]
  • URL: http://home.hiroshima-u.ac.jp/physiol2/