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Development of a new single-cell imaging method using oxonol voltage-sensitive dyes

Research Project

Project/Area Number 22300129
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurophysiology and muscle physiology
Research InstitutionKanto Gakuin University

Principal Investigator

MOMOSE-SATO Yoko  関東学院大学, 人間環境学部, 教授 (70251501)

Co-Investigator(Renkei-kenkyūsha) SATO Katsushige  駒沢女子大学, 人間健康学部, 教授 (80291342)
Project Period (FY) 2010-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Keywords膜電位感受性色素 / イメージング / 鶏胚 / 細胞 / 神経系
Outline of Final Research Achievements

We evaluated the suitability of voltage-sensitive oxonol dyes for optical imaging at the single-cell level. We screened four oxonol dyes in dissociated cells from 8-9 day-old embryonic chick brainstems. Voltage-related fluorescent changes in response to the perfusion of a high-K+ solution were measured using a cooled CCD camera. Clear optical responses were detected with every dye, suggesting the potential utility of the oxonol dye in optical imaging of voltage changes at the single-cell level.

Report

(6 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (43 results)

All 2015 2014 2013 2012 2011 2010 Other

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Acknowledgement Compliant: 3 results,  Open Access: 1 results) Presentation (27 results) (of which Invited: 1 results) Book (3 results) Remarks (2 results)

  • [Journal Article] Voltage-sensitive dye imaging during functional development of the embryonic nervous system: a brief review with special thanks to Professor Larry Cohen.2015

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Journal Title

      Neurophotonics

      Volume: 2 Issue: 2 Pages: 0210091-5

    • DOI

      10.1117/1.nph.2.2.021009

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Maintenance of the large-scale depolarization wave in the embryonic chick brain against deprivation of the rhythm generator.2014

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Journal Title

      Neuroscience

      Volume: 266 Pages: 186-196

    • DOI

      10.1016/j.neuroscience.2014.02.014

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Optical survey of initial expression of synaptic function in the embryonic chick trigeminal sensory nucleus.2014

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Journal Title

      Neuroscience Letters

      Volume: 570 Pages: 92-96

    • DOI

      10.1016/j.neulet.2014.04.017

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Optical survey of vagus nerve-related neuronal circuits in the embryonic rat brainstem.2013

    • Author(s)
      Momose-Sato, Y., Nakamori, T., Mullah, S. H.- E.-R. and Sato, K.
    • Journal Title

      Neuroscience Letters

      Volume: 535 Pages: 140-145

    • DOI

      10.1016/j.neulet.2012.12.013

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of voltage-sensitive fluorescence dyes for monitoring neuronal activity in the embryonic central nervous system.2013

    • Author(s)
      Mullah, S. H.-E.-R., Komuro, R., Yan, P., Hayashi, S., Inaji, M., Momose- Sato, Y., Loew, L. M. and Sato, K.
    • Journal Title

      The Journal of Membrane Biology

      Volume: 246 Issue: 9 Pages: 679-688

    • DOI

      10.1007/s00232-013-9584-1

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical imaging of the spontaneous depolarization wave in the mouse embryo: Origins and pharmacological natures.2013

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Journal Title

      Annals of the New York Academy of Sciences

      Volume: 1279 Issue: 1 Pages: 60-70

    • DOI

      10.1111/j.1749-6632.2012.06806.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Large-scale synchronized activity in the embryonic brainstem and spinal cord.2013

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Journal Title

      Frontiers in Cellular Neuroscience

      Volume: 7, Article 36 Pages: 1-15

    • DOI

      10.3389/fncel.2013.00036

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous depolarization wave in the mouse embryo: origin and large-scale propagation over the CNS identified with voltage-sensitive dye imaging.2012

    • Author(s)
      Momose-Sato, Y., Nakamori, T. and Sato, K.
    • Journal Title

      European Journal of Neuroscience

      Volume: 35 Issue: 8 Pages: 1230-1241

    • DOI

      10.1111/j.1460-9568.2012.07997.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pharmacological mechanisms underlying the switching from the large-scale depolarization wave to segregated activity in the mouse CNS.2012

    • Author(s)
      Momose-Sato, Y., Nakamori, T. and Sato, K.
    • Journal Title

      European Journal of Neuroscience

      Volume: 35 Issue: 8 Pages: 1242-1252

    • DOI

      10.1111/j.1460-9568.2012.08040.x

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical analysis of developmental changes in synaptic potentiation in the neonatal rat corticostriatal projection2012

    • Author(s)
      Mullah, S.H.-E.-R., et al
    • Journal Title

      Neuroscience

      Volume: 201 Pages: 338-348

    • DOI

      10.1016/j.neuroscience.2011.11.023

    • Related Report
      2012 Annual Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Voltage-sensitive dye imaging analysis of functional development of the neonatal rat cortocostriatal projection2011

    • Author(s)
      Inaji, M., et al.
    • Journal Title

      Neurolmage

      Volume: 54 Pages: 1831-1839

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Optical survey of initial expression of synaptic function in the embryonic chick trigeminal sensory nucleus.2014

    • Author(s)
      Sato, K. and Momose-Sato, Y.
    • Organizer
      Society for Neuroscience 44th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Maintenance of the large-scale depolarization wave in the embryonic chick brain against deprivation of the rhythm generator.2014

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Organizer
      Society for Neuroscience 44th Annual Meeting
    • Place of Presentation
      Washington DC, USA
    • Year and Date
      2014-11-15 – 2014-11-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Optical survey of initial expression of synaptic function in the embryonic chick trigeminal sensory nucleus.2014

    • Author(s)
      Sato, K. and Momose-Sato, Y.
    • Organizer
      The 37th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Homeostatic maintenance of the large-scale depolarization wave in the developing central nervous system.2014

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Organizer
      The 37th Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      横浜
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Annual Research Report
  • [Presentation] Optical survey of initial expression of synaptic function in the embryonic chick trigeminal sensory nucleus.2014

    • Author(s)
      Sato, K. and Momose-Sato, Y.
    • Organizer
      The 91st Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Kagoshima
    • Related Report
      2013 Annual Research Report
  • [Presentation] Homeostatic maintenance of the large-scale depolarization wave in the developing central nervous system.2014

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Organizer
      The 91st Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Kagoshima
    • Related Report
      2013 Annual Research Report
  • [Presentation] Voltage-sensitive dye imaging of neural activities in the embryonic central nervous system.2014

    • Author(s)
      Sato, K. and Momose-Sato, Y.
    • Organizer
      JThe 91st Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      Kagoshima
    • Related Report
      2013 Annual Research Report
  • [Presentation] Membrane potential imaging during functional development of neuronal circuits in vertebrate embryos.2013

    • Author(s)
      Momose-Sato, Y. and Sato, K.
    • Organizer
      XXXVII International Congress of Physiological Sciences (IUPS 2013)
    • Place of Presentation
      Birmingham, UK
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Large-scale correlated wave in the embryonic mouse CNS: Development, origins and pharmacological natures.2013

    • Author(s)
      Sato, K. and Momose-Sato, Y.
    • Organizer
      XXXVII International Congress of Physiological Sciences (IUPS 2013)
    • Place of Presentation
      Birmingham, UK
    • Related Report
      2013 Annual Research Report
  • [Presentation] Evaluation of voltage-sensitive fluorescence dyes for monitoring neuronal activity in the embryonic CNS.2013

    • Author(s)
      Mullah, S. H.-E.-R. , Komuro, R., Yan, P., Inaji, M., Urmy, J., Momose-Sato, Y., Loew, L. M. and Sato, K.
    • Organizer
      Society for Neuroscience 43rd Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Effects of in ovo blockade of the spontaneous depolarization wave on functional synaptogenesis in the developing CNS.2013

    • Author(s)
      Momose-Sato, Y., Tashiro, Y. and Sato, K.
    • Organizer
      The 36 Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      Kyoto
    • Related Report
      2013 Annual Research Report
  • [Presentation] Effects of in ovo blockade of the spontaneous depolarization wave on functional synaptogenesis in the embryonic NTS.2013

    • Author(s)
      Momose-Sato, Y., Tashiro, Y., Nakamori, T., Mullah H.-E-R. S. and Sato, K.
    • Organizer
      The 90th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      タワーホール船堀(東京)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Developmental changes in the LTP in the neonatal rat corticostriatal projection: Optical imaging with the voltage-sensitive dye.2013

    • Author(s)
      Sato, K., Mullah H.-E-R. S., Inaji, M., Nariai, T., Momose-Sato, Y. and Ohno, K.
    • Organizer
      The 90th Annual Meeting of the Physiological Society of Japan
    • Place of Presentation
      タワーホール船堀(東京)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Pharmacological nature of the depolarization wave in the mouse CNS-Dual switching and its developmental significance-2012

    • Author(s)
      Momose-Sato, Y., et al
    • Organizer
      日本生理学会
    • Place of Presentation
      松本文化会館(松本市)
    • Year and Date
      2012-03-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] From embryonic correlated activity to the respiratory and locomotor rhythms: pharmacological mechanisms underlying the switching.2012

    • Author(s)
      Momose-Sato, Y., Nakamori, T., Mullah H.-E-R. S. and Sato, K.
    • Organizer
      The 35 Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Optical analysis of developmental changes in the LTP in the neonatal rat corticostriatal projection.2012

    • Author(s)
      Sato, K., Mullah H.-E-R. S., Inaji, M., Nariai, T., Momose-Sato, Y. and Ohno, K.
    • Organizer
      The 35 Annual Meeting of the Japan Neuroscience Society
    • Place of Presentation
      名古屋国際会議場(名古屋)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Effects of in ovo blockade of the spontaneous depolarization wave on functional synaptogenesis in the embryonic brainstem nucleus.2012

    • Author(s)
      Momose-Sato, Y., Tashiro, Y., Nakamori, T., Mullah, S. H.-E.-R. and Sato, K.
    • Organizer
      Society for Neuroscience 42nd Annual Meeting
    • Place of Presentation
      Ernest N, Memorial Convention Center New Orleans (USA)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Developmental changes in the LTP in the neonatal rat corticostriatal projection: Optical imaging with a voltage-sensitive dye.2012

    • Author(s)
      Mullah, S. H.-E.-R. , Inaji, M., Nariai, T., Urmy, J., Momose-Sato, Y., Ohno, K. and Sato, K.
    • Organizer
      Society for Neuroscience 42nd Annual Meeting
    • Place of Presentation
      Ernest N, Memorial Convention Center New Orleans (USA)
    • Related Report
      2012 Annual Research Report
  • [Presentation] Dual switching in the pharmacological nature of the large-scale depolarization wave in the mouse embryo2011

    • Author(s)
      Momose-Sato, Y., et al
    • Organizer
      Society for Neuroscience
    • Place of Presentation
      Washington DC Convention Center (Washington DC, USA)
    • Year and Date
      2011-11-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] Spontaneous depolarization wave in the embryonic mouse CNS : disappearance of the wave with development and its mechanism2011

    • Author(s)
      Momose-Sato, Y., et al
    • Organizer
      日本神経科学学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Screening of voltage-sensitive fluorescence dyes for imaging embryonicneural activity2011

    • Author(s)
      Sato, K., et al
    • Organizer
      日本神経科学学会
    • Place of Presentation
      パシフィコ横浜(横浜市)
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Spontaneous depolarization wave in the mouse embryo : spatial segregation with development and its mechanism2011

    • Author(s)
      Momose-Sato, Y., et al
    • Organizer
      日本生理学会
    • Place of Presentation
      (東日本大震災のため、誌上開催)(Journal of Physiological Sciences 61 suppl.1,S168)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Screening of fluorescent voltage-sensitive dyes for detecting neural activity in the embryonic brain2011

    • Author(s)
      Sato, K., et al
    • Organizer
      日本生理学会
    • Place of Presentation
      (東日本大震災のため、誌上開催)(Journal of Physiological Sciences 61 suppl.1,S168)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Developmental changes in the spontaneous depolarization wave in the mouse embryo : mechanisms underlying spatial segregation.2010

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Organizer
      Society for Neuroscience 40th Annual Meeting
    • Place of Presentation
      San Diego Convention Center (San Diego, USA)
    • Year and Date
      2010-11-14
    • Related Report
      2010 Annual Research Report
  • [Presentation] 発生期マウス中枢神経系における自発性広範囲伝播脱分極波の光学的解析2010

    • Author(s)
      佐藤容子、佐藤勝重
    • Organizer
      第33回日本神経科学大会
    • Place of Presentation
      神戸コンベンションセンター(神戸市)
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] 発生期マウス中枢神経系における自発性広範囲伝播脱分極波の光学的イメージング2010

    • Author(s)
      佐藤容子、佐藤勝重
    • Organizer
      第87回日本生理学会
    • Place of Presentation
      盛岡市民文化ホール(盛岡市)
    • Year and Date
      2010-05-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] 胎生期中枢神経回路に発現する広範囲律動性脱分極波2010

    • Author(s)
      佐藤勝重、佐藤容子
    • Organizer
      第87回日本生理学会
    • Place of Presentation
      盛岡市民文化ホール(盛岡市)
    • Year and Date
      2010-05-20
    • Related Report
      2010 Annual Research Report
  • [Book] Membrane Potential Imaging in the Nervous System and Heart2015

    • Author(s)
      Momose-Sato, Y., Sato, K. and Kamino, K.
    • Publisher
      Springer-Verlag, New York
    • Related Report
      2014 Annual Research Report
  • [Book] Membrane Potential Imaging in the Nervous System : Methods and Applications.2011

    • Author(s)
      Momose-Sato, Y., et al.
    • Publisher
      Springer-Verlag
    • Related Report
      2010 Annual Research Report
  • [Book] Comprehensive Developmental Neuroscience.2011

    • Author(s)
      Momose-Sato, Y., Sato, K.
    • Publisher
      Elsevier(in press)
    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/optical/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://square.umin.ac.jp/optical/

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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