Development of a new single-cell imaging method using oxonol voltage-sensitive dyes

• Project/Area Number: 22300129
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Kanto Gakuin University
• Principal Investigator: MOMOSE-SATO Yoko 関東学院大学, 人間環境学部, 教授 (70251501)
• Co-Investigator(Renkei-kenkyūsha): SATO Katsushige 駒沢女子大学, 人間健康学部, 教授 (80291342)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
• Keywords: 膜電位感受性色素 / イメージング / 鶏胚 / 細胞 / 神経系

Outline of Final Research Achievements

We evaluated the suitability of voltage-sensitive oxonol dyes for optical imaging at the single-cell level. We screened four oxonol dyes in dissociated cells from 8-9 day-old embryonic chick brainstems. Voltage-related fluorescent changes in response to the perfusion of a high-K+ solution were measured using a cooled CCD camera. Clear optical responses were detected with every dye, suggesting the potential utility of the oxonol dye in optical imaging of voltage changes at the single-cell level.

Research Products

• [Journal Article] Voltage-sensitive dye imaging during functional development of the embryonic nervous system: a brief review with special thanks to Professor Larry Cohen. (2015)
  • Author(s): Momose-Sato, Y. and Sato, K.
  • Journal Title: Neurophotonics Volume: 2 Issue: 2 Pages: 0210091-5
  • DOI: 10.1117/1.nph.2.2.021009
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Maintenance of the large-scale depolarization wave in the embryonic chick brain against deprivation of the rhythm generator. (2014)
  • Author(s): Momose-Sato, Y. and Sato, K.
  • Journal Title: Neuroscience Volume: 266 Pages: 186-196
  • DOI: 10.1016/j.neuroscience.2014.02.014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Optical survey of initial expression of synaptic function in the embryonic chick trigeminal sensory nucleus. (2014)
  • Author(s): Momose-Sato, Y. and Sato, K.
  • Journal Title: Neuroscience Letters Volume: 570 Pages: 92-96
  • DOI: 10.1016/j.neulet.2014.04.017
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Optical survey of vagus nerve-related neuronal circuits in the embryonic rat brainstem. (2013)
  • Author(s): Momose-Sato, Y., Nakamori, T., Mullah, S. H.- E.-R. and Sato, K.
  • Journal Title: Neuroscience Letters Volume: 535 Pages: 140-145
  • DOI: 10.1016/j.neulet.2012.12.013
  • Peer Reviewed
• [Journal Article] Evaluation of voltage-sensitive fluorescence dyes for monitoring neuronal activity in the embryonic central nervous system. (2013)
  • Author(s): Mullah, S. H.-E.-R., Komuro, R., Yan, P., Hayashi, S., Inaji, M., Momose- Sato, Y., Loew, L. M. and Sato, K.
  • Journal Title: The Journal of Membrane Biology Volume: 246 Issue: 9 Pages: 679-688
  • DOI: 10.1007/s00232-013-9584-1
  • Peer Reviewed
• [Journal Article] Optical imaging of the spontaneous depolarization wave in the mouse embryo: Origins and pharmacological natures. (2013)
  • Author(s): Momose-Sato, Y. and Sato, K.
  • Journal Title: Annals of the New York Academy of Sciences Volume: 1279 Issue: 1 Pages: 60-70
  • DOI: 10.1111/j.1749-6632.2012.06806.x
  • Peer Reviewed
• [Journal Article] Large-scale synchronized activity in the embryonic brainstem and spinal cord. (2013)
  • Author(s): Momose-Sato, Y. and Sato, K.
  • Journal Title: Frontiers in Cellular Neuroscience Volume: 7, Article 36 Pages: 1-15
  • DOI: 10.3389/fncel.2013.00036
  • Peer Reviewed
• [Journal Article] Spontaneous depolarization wave in the mouse embryo: origin and large-scale propagation over the CNS identified with voltage-sensitive dye imaging. (2012)
  • Author(s): Momose-Sato, Y., Nakamori, T. and Sato, K.
  • Journal Title: European Journal of Neuroscience Volume: 35 Issue: 8 Pages: 1230-1241
  • DOI: 10.1111/j.1460-9568.2012.07997.x
  • Peer Reviewed
• [Journal Article] Pharmacological mechanisms underlying the switching from the large-scale depolarization wave to segregated activity in the mouse CNS. (2012)
  • Author(s): Momose-Sato, Y., Nakamori, T. and Sato, K.
  • Journal Title: European Journal of Neuroscience Volume: 35 Issue: 8 Pages: 1242-1252
  • DOI: 10.1111/j.1460-9568.2012.08040.x
  • Peer Reviewed
• [Journal Article] Optical analysis of developmental changes in synaptic potentiation in the neonatal rat corticostriatal projection (2012)
  • Author(s): Mullah, S.H.-E.-R., et al
  • Journal Title: Neuroscience Volume: 201 Pages: 338-348
  • DOI: 10.1016/j.neuroscience.2011.11.023
  • Peer Reviewed
• [Journal Article] Voltage-sensitive dye imaging analysis of functional development of the neonatal rat cortocostriatal projection (2011)
  • Author(s): Inaji, M., et al.
  • Journal Title: Neurolmage Volume: 54 Pages: 1831-1839
  • Peer Reviewed
• [Presentation] Optical survey of initial expression of synaptic function in the embryonic chick trigeminal sensory nucleus. (2014)
  • Author(s): Sato, K. and Momose-Sato, Y.
  • Organizer: Society for Neuroscience 44th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] Maintenance of the large-scale depolarization wave in the embryonic chick brain against deprivation of the rhythm generator. (2014)
  • Author(s): Momose-Sato, Y. and Sato, K.
  • Organizer: Society for Neuroscience 44th Annual Meeting
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2014-11-15 – 2014-11-19
• [Presentation] Optical survey of initial expression of synaptic function in the embryonic chick trigeminal sensory nucleus. (2014)
  • Author(s): Sato, K. and Momose-Sato, Y.
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Homeostatic maintenance of the large-scale depolarization wave in the developing central nervous system. (2014)
  • Author(s): Momose-Sato, Y. and Sato, K.
  • Organizer: The 37th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Optical survey of initial expression of synaptic function in the embryonic chick trigeminal sensory nucleus. (2014)
  • Author(s): Sato, K. and Momose-Sato, Y.
  • Organizer: The 91st Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Kagoshima
• [Presentation] Homeostatic maintenance of the large-scale depolarization wave in the developing central nervous system. (2014)
  • Author(s): Momose-Sato, Y. and Sato, K.
  • Organizer: The 91st Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Kagoshima
• [Presentation] Voltage-sensitive dye imaging of neural activities in the embryonic central nervous system. (2014)
  • Author(s): Sato, K. and Momose-Sato, Y.
  • Organizer: JThe 91st Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Kagoshima
• [Presentation] Membrane potential imaging during functional development of neuronal circuits in vertebrate embryos. (2013)
  • Author(s): Momose-Sato, Y. and Sato, K.
  • Organizer: XXXVII International Congress of Physiological Sciences (IUPS 2013)
  • Place of Presentation: Birmingham, UK
  • Invited
• [Presentation] Large-scale correlated wave in the embryonic mouse CNS: Development, origins and pharmacological natures. (2013)
  • Author(s): Sato, K. and Momose-Sato, Y.
  • Organizer: XXXVII International Congress of Physiological Sciences (IUPS 2013)
  • Place of Presentation: Birmingham, UK
• [Presentation] Evaluation of voltage-sensitive fluorescence dyes for monitoring neuronal activity in the embryonic CNS. (2013)
  • Author(s): Mullah, S. H.-E.-R. , Komuro, R., Yan, P., Inaji, M., Urmy, J., Momose-Sato, Y., Loew, L. M. and Sato, K.
  • Organizer: Society for Neuroscience 43rd Annual Meeting
  • Place of Presentation: San Diego, USA
• [Presentation] Effects of in ovo blockade of the spontaneous depolarization wave on functional synaptogenesis in the developing CNS. (2013)
  • Author(s): Momose-Sato, Y., Tashiro, Y. and Sato, K.
  • Organizer: The 36 Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kyoto
• [Presentation] Effects of in ovo blockade of the spontaneous depolarization wave on functional synaptogenesis in the embryonic NTS. (2013)
  • Author(s): Momose-Sato, Y., Tashiro, Y., Nakamori, T., Mullah H.-E-R. S. and Sato, K.
  • Organizer: The 90th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: タワーホール船堀（東京）
• [Presentation] Developmental changes in the LTP in the neonatal rat corticostriatal projection: Optical imaging with the voltage-sensitive dye. (2013)
  • Author(s): Sato, K., Mullah H.-E-R. S., Inaji, M., Nariai, T., Momose-Sato, Y. and Ohno, K.
  • Organizer: The 90th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: タワーホール船堀（東京）
• [Presentation] Pharmacological nature of the depolarization wave in the mouse CNS-Dual switching and its developmental significance- (2012)
  • Author(s): Momose-Sato, Y., et al
  • Organizer: 日本生理学会
  • Place of Presentation: 松本文化会館(松本市)
  • Year and Date: 2012-03-29
• [Presentation] From embryonic correlated activity to the respiratory and locomotor rhythms: pharmacological mechanisms underlying the switching. (2012)
  • Author(s): Momose-Sato, Y., Nakamori, T., Mullah H.-E-R. S. and Sato, K.
  • Organizer: The 35 Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋国際会議場（名古屋）
• [Presentation] Optical analysis of developmental changes in the LTP in the neonatal rat corticostriatal projection. (2012)
  • Author(s): Sato, K., Mullah H.-E-R. S., Inaji, M., Nariai, T., Momose-Sato, Y. and Ohno, K.
  • Organizer: The 35 Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 名古屋国際会議場（名古屋）
• [Presentation] Effects of in ovo blockade of the spontaneous depolarization wave on functional synaptogenesis in the embryonic brainstem nucleus. (2012)
  • Author(s): Momose-Sato, Y., Tashiro, Y., Nakamori, T., Mullah, S. H.-E.-R. and Sato, K.
  • Organizer: Society for Neuroscience 42nd Annual Meeting
  • Place of Presentation: Ernest N, Memorial Convention Center New Orleans (USA)
• [Presentation] Developmental changes in the LTP in the neonatal rat corticostriatal projection: Optical imaging with a voltage-sensitive dye. (2012)
  • Author(s): Mullah, S. H.-E.-R. , Inaji, M., Nariai, T., Urmy, J., Momose-Sato, Y., Ohno, K. and Sato, K.
  • Organizer: Society for Neuroscience 42nd Annual Meeting
  • Place of Presentation: Ernest N, Memorial Convention Center New Orleans (USA)
• [Presentation] Dual switching in the pharmacological nature of the large-scale depolarization wave in the mouse embryo (2011)
  • Author(s): Momose-Sato, Y., et al
  • Organizer: Society for Neuroscience
  • Place of Presentation: Washington DC Convention Center (Washington DC, USA)
  • Year and Date: 2011-11-14
• [Presentation] Spontaneous depolarization wave in the embryonic mouse CNS : disappearance of the wave with development and its mechanism (2011)
  • Author(s): Momose-Sato, Y., et al
  • Organizer: 日本神経科学学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-09-16
• [Presentation] Screening of voltage-sensitive fluorescence dyes for imaging embryonicneural activity (2011)
  • Author(s): Sato, K., et al
  • Organizer: 日本神経科学学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-09-16
• [Presentation] Spontaneous depolarization wave in the mouse embryo : spatial segregation with development and its mechanism (2011)
  • Author(s): Momose-Sato, Y., et al
  • Organizer: 日本生理学会
  • Place of Presentation: (東日本大震災のため、誌上開催)(Journal of Physiological Sciences 61 suppl.1,S168)
• [Presentation] Screening of fluorescent voltage-sensitive dyes for detecting neural activity in the embryonic brain (2011)
  • Author(s): Sato, K., et al
  • Organizer: 日本生理学会
  • Place of Presentation: (東日本大震災のため、誌上開催)(Journal of Physiological Sciences 61 suppl.1,S168)
• [Presentation] Developmental changes in the spontaneous depolarization wave in the mouse embryo : mechanisms underlying spatial segregation. (2010)
  • Author(s): Momose-Sato, Y., Sato, K.
  • Organizer: Society for Neuroscience 40th Annual Meeting
  • Place of Presentation: San Diego Convention Center (San Diego, USA)
  • Year and Date: 2010-11-14
• [Presentation] 発生期マウス中枢神経系における自発性広範囲伝播脱分極波の光学的解析 (2010)
  • Author(s): 佐藤容子、佐藤勝重
  • Organizer: 第33回日本神経科学大会
  • Place of Presentation: 神戸コンベンションセンター(神戸市)
  • Year and Date: 2010-09-02
• [Presentation] 発生期マウス中枢神経系における自発性広範囲伝播脱分極波の光学的イメージング (2010)
  • Author(s): 佐藤容子、佐藤勝重
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡市民文化ホール(盛岡市)
  • Year and Date: 2010-05-21
• [Presentation] 胎生期中枢神経回路に発現する広範囲律動性脱分極波 (2010)
  • Author(s): 佐藤勝重、佐藤容子
  • Organizer: 第87回日本生理学会
  • Place of Presentation: 盛岡市民文化ホール(盛岡市)
  • Year and Date: 2010-05-20
• [Book] Membrane Potential Imaging in the Nervous System and Heart (2015)
  • Author(s): Momose-Sato, Y., Sato, K. and Kamino, K.
  • Publisher: Springer-Verlag, New York
• [Book] Membrane Potential Imaging in the Nervous System : Methods and Applications. (2011)
  • Author(s): Momose-Sato, Y., et al.
  • Publisher: Springer-Verlag
• [Book] Comprehensive Developmental Neuroscience. (2011)
  • Author(s): Momose-Sato, Y., Sato, K.
  • Publisher: Elsevier(in press)
• [Remarks]
  • URL: http://square.umin.ac.jp/optical/
• [Remarks]
  • URL: http://square.umin.ac.jp/optical/