Visualization analysis of neurotransmission and exocytosis by using novel fluorescent protein and laser optics technology
Project/Area Number |
22300131
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Fusional basic brain science
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Research Institution | Hokkaido University |
Principal Investigator |
NEMOTO Tomomi 北海道大学, 電子科学研究所, 教授 (50291084)
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Project Period (FY) |
2010 – 2012
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Project Status |
Completed (Fiscal Year 2012)
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Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2011: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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Keywords | 2光子顕微鏡 / 分泌 / 蛍光タンパク質 / SNARE / 光脳科学 / 非線形光学 / 開口放出 / イメージング / 2光子顕微鏡強 / 破骨細胞 / SNAREタンパク質 |
Research Abstract |
We have developed novel visualization analysis techniques for neurotransmission and exocytosis, and constructed “in vivo” optical imaging methods to advance for elucidation of the molecular basis. We generated fluorescently tagged SNARE proteins, VAMP-Sirius by using a novel fluorescent protein Sirius in order to visualize dynamics of acid vesicles in an osteoclast model. A novel FRET Ca^<2+> sensor, Cameleon-nano protein, visualized Ca^<2+>-dependent exocyosis in pancreatic acinar cells, as well as Ca^<2+> dynamics in neurons in cerebellum. Furthermore, we successfully observed hippocampal neurons in deeper layers in live mouse brains.
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Report
(4 results)
Research Products
(162 results)
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[Journal Article] A novel function of Noc2 in agonist-induced intracellular Ca2+ increase during zymogen-granule exocytosis in pancreatic acinar cells.2012
Author(s)
Ogata, S., Miki, T., Seino, S., Tamai, S., Kasai, H. & Nemoto, T.
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Journal Title
PLoS ONE
Volume: e37048
Issue: 5
Pages: 1-9
DOI
Related Report
Peer Reviewed
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[Journal Article] Rap1 controls lymphocyte adhesion cascade and interstitial migration within lymph nodes in a RAPL-dependent and-independent manner2010
Author(s)
Yukihiko Ebisuno, Koko Katagiri, Tomoya Katakai, Yoshihiro Ueda, Tomomi Nemoto, Hiroyuki Inada, Junichi Nabekura, Takaharu Okada, Reiji Kannagi, Toshiyuki Tanaka, Masayuki Miyasaka, Nancy Hogg, Tatsuo Kinashi
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Journal Title
Blood
Volume: 115(4)
Pages: 804-14
Related Report
Peer Reviewed
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[Presentation] In vivo two-photon microscopy with a 1030 nm (high-peak-power) picosecond-pulse laser to visualize the cortexand hippocampal pyramidal neurons in H-Line mice2012
Author(s)
R. Kawakami, K. Sawada, A. Sato, T. Hibi, Y.Kozawa, S. Sato, H. Yokoyama, T. Nemoto
Organizer
Society for Neuroscience
Place of Presentation
Ernest N. Morial Convention Center, New Orleans, US, 2012
Related Report
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[Presentation] Improvement of the Spatial Resolution in Two-Photon Microscopy with a Vector Beam Generated by Liquid Crystal Devices2012
Author(s)
S. Ipponjima, T. Terumasa, Y. Kozawa , H.Horanai, A. Sato, M. Kurihara, N. Hashimoto, H. Yokoyama, S. Sato, T. Nemoto
Organizer
Focus On Microscopy 2012, Suntec Singapore International Convention & Exhibition centre
Place of Presentation
Singapore, Republic of Singapore
Related Report
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[Presentation] 脳をみる
Author(s)
根本知己
Organizer
光材料・応用技術研究会 第四回研究会
Place of Presentation
東京理科大(東京)
Related Report
Invited
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