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Molecular mechanisms of the sleep control by neurons activated during sleep

Research Project

Project/Area Number 22300133
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Fusional basic brain science
Research Institution公益財団法人大阪バイオサイエンス研究所 (2012)
Osaka Bioscience Institute (2010-2011)

Principal Investigator

URADE Yoshihiro  公益財団法人大阪バイオサイエンス研究所, 分子行動生物学部門, 研究部長 (10201360)

Co-Investigator(Kenkyū-buntansha) FUJIMORI Ko  大阪薬科大学, 薬学部, 講師 (70425453)
Co-Investigator(Renkei-kenkyūsha) KASHIWAGI Kaori  (公財)大阪バイオサイエンス研究所, 分子行動生物学部門, 研究員 (10372666)
NAGATA Nanae  (公財)大阪バイオサイエンス研究所, 分子行動生物学部門, 研究員 (80390805)
ZHI Li-huang  (公財)大阪バイオサイエンス研究所, 分子行動生物学部門, 研究副部長 (10321704)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords睡眠 / Sox5 / 神経 / SOX5
Research Abstract

In this study, we identified a novel splicing isoform of Sox5 with truncation at exon 2, Sox5-t2. Q-PCR analysis revealed that Sox5-t2 was dominantly expressed in the adult mouse cortex. Electrophoretic mobility shift assay showed that Sox5-t2 binds to genes that are up-regulated during sleep and contain Sox5-binding elements. By conditional knockout of Sox5 in the forebrain, the amount of REM sleep decreased in the light phase. These results suggest that Sox5 is an important regulator of sleep-related functions of neurons.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (31 results)

All 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 3 results) Presentation (21 results) Remarks (1 results)

  • [Journal Article] Role of the basal ganglia in the control of sleep and wakefulness2013

    • Author(s)
      Lazarus, M.; Chen, J. F.; Urade, Y.; Huang, Z. L.
    • Journal Title

      Curr Opin Neurobiol

      Volume: 23 Issue: 5 Pages: 780-785

    • DOI

      10.1016/j.conb.2013.02.001

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sleep-wake regulation by prostaglandin D2 and adenosine2012

    • Author(s)
      Nagata, N.; Urade, Y
    • Journal Title

      Brain Nerve

      Volume: 64 Pages: 621-8

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22647469.

    • Related Report
      2012 Final Research Report
  • [Journal Article] Endogenous sleep-promoting substance2012

    • Author(s)
      Nagata, N.; Urade, Y.
    • Journal Title

      Nihon Rinsho

      Volume: 70 (7) Pages: 1227-32

    • NAID

      40019360568

    • Related Report
      2012 Final Research Report
  • [Journal Article] How do the basal ganglia regulate sleep-wake behavior?2012

    • Author(s)
      Lazarus, M.; Huang, Z. L.; Lu, J.; Urade, Y.; Chen, J. F.
    • Journal Title

      Trends Neurosci

      Volume: 35(12) Pages: 723-32

    • Related Report
      2012 Final Research Report
  • [Journal Article] Prostaglandin D2 and sleep/wake regulation2011

    • Author(s)
      Urade, Y.; Hayaishi, O.
    • Journal Title

      Sleep Med Rev

      Volume: 15 (6) Issue: 6 Pages: 411-8

    • DOI

      10.1016/j.smrv.2011.08.003

    • Related Report
      2012 Final Research Report
  • [Journal Article] Hematopoietic prostaglandin D synthase (H-Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein2011

    • Author(s)
      Moniot, B.; Farhat, A.; Aritake, K.; Declosmenil, F.; Nef, S.; Eguchi, N.; Urade, Y.; Poulat, F.; Boizet-Bonhoure, B.
    • Journal Title

      Dev Dyn

      Volume: 240 (10) Issue: 10 Pages: 2335-43

    • DOI

      10.1002/dvdy.22726

    • Related Report
      2012 Final Research Report
  • [Journal Article] The role of adenosine in the regulation of sleep2011

    • Author(s)
      Huang, Z. L.; Urade, Y.; Hayaishi, O.
    • Journal Title

      Curr Top Med Chem

      Volume: 11 (8) Pages: 1047-57

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/21401496.

    • Related Report
      2012 Final Research Report
  • [Journal Article] Prostaglandin D_<2>and Sleep/Wake Regulation2011

    • Author(s)
      Urade, Y, Hayaishi, O
    • Journal Title

      Sleep Medicine reviews

      Volume: In press

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crucial role of prostaglandin D_<2>and adenosine in sleep regulation-Experimental evidence from pharmacological approaches to gene-knockoutmice.2010

    • Author(s)
      Urade, Y, Hayaishi, O
    • Journal Title

      Future Neurology

      Volume: 5(3) Pages: 363-376

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] マウス脳における脂肪酸 結合タンパク質FABP7 の相互作用タンパ ク質の同定2012

    • Author(s)
      永田奈々恵、柏木香保里、山本利義、三 田村エリザベス、ミハエル・ラザルス、 黄 志力、藤森 功、宮本悦子、裏出良 博
    • Organizer
      第85回日本生化学大会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-15
    • Related Report
      2012 Final Research Report
  • [Presentation] Prostaglandin D2 involved in sleep regulation is produced in the leptomeninges of brain2012

    • Author(s)
      Yoan Cherasse, Michael Lazarus, Yo Oishi, Mahesh Kaushik, Yoshihiro Urade, Osamu Hayaishi
    • Organizer
      The 7^<th> Asian Sleep Research Society Congress
    • Place of Presentation
      Taipei, 台湾
    • Year and Date
      2012-11-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Key roles of dopamine D2 receptor in the sleep-wake regulation2012

    • Author(s)
      Zhi-Li Huang, Wei-Min Qu. Yoshihiro Urade
    • Organizer
      The 7^<th> Asian Sleep Research Society Congress
    • Place of Presentation
      Taipei, 台湾
    • Year and Date
      2012-11-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Role of PGD2 and adenosine in sleep regulation: from pharmacological approaches to gene-knockout mice2012

    • Author(s)
      Yoshihiro Urade
    • Organizer
      The 7th National Sleep Congress of Chinese Sleep Research Spciety
    • Place of Presentation
      Nanjing, China
    • Year and Date
      2012-10-20
    • Related Report
      2012 Final Research Report
  • [Presentation] Sleep is regulated by prostaglandin D2 selectively produced in the meninges by lipocalin-type prostaglandin D synthase2012

    • Author(s)
      Yoan Cherasse, Yo Oishi, Michael Lazarus, Zhi-Li Huang, Osamu Hayaishi, Yoshihiro Urade
    • Organizer
      22^<nd> IUBMB
    • Place of Presentation
      Sevilla, Spain
    • Year and Date
      2012-09-06
    • Related Report
      2012 Final Research Report
  • [Presentation] 睡眠基礎研究 ‐動物から人へ‐2012

    • Author(s)
      裏出良博
    • Organizer
      第12回日本抗加齢医学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2012-06-24
    • Related Report
      2012 Final Research Report
  • [Presentation] Hypothalamic regulation of sleep-wake cycle, from basic research to drug discovery2012

    • Author(s)
      Zhi-Li Huang, Wei-Min Qu, Yoshihiro Urade
    • Organizer
      The 2012 Beijing Sleep Medicine Forum
    • Place of Presentation
      Beijing, China
    • Year and Date
      2012-05-13
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel SOX5 splicing isoform expressed in mouse brain during sleep2011

    • Author(s)
      Nanae Nagata, Kaori Kashiwagi, Toshiyoshi Yamamoto, Elizabeth P.Ko-Mitamura, Michael Lazarus, Zhi-Li-Huang, Ko Fujimori, Yoshihiro Urade
    • Organizer
      World Sleep 2011
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Dopamine D2 receptors in the basal ganglia are essential in the maintenance of wakefulness2011

    • Author(s)
      Z.-L. Huang, W.-M. Qu, Yoshihiro Urade
    • Organizer
      8^<th< IBRO World Congress of Neuroscience
    • Place of Presentation
      Florence, Italy
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of adenosine A2A receptors in the nucleus accumbens for sleep-wake regulation2011

    • Author(s)
      Michael Lazarus, Yoshihiro Urade
    • Organizer
      8^<th> IBRO WorldCongress of Neuroscience
    • Place of Presentation
      Florence, Italy
    • Related Report
      2012 Final Research Report
  • [Presentation] Molecular mechanism of sleep-wake regulation: From basic research to translational research2011

    • Author(s)
      Yoshihiro Urade
    • Organizer
      21st World Congress on Psychosomatic Medicine
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of the nucleus accumbens in the regulation of behavioral arousal2011

    • Author(s)
      Michael Lazarus, Yoshihiro Urade
    • Organizer
      21st World Congress on Psychosomatic Medicine
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2012 Final Research Report
  • [Presentation] Humoral and neural regulation of sleep - Lesson from prostaglandin D2-induced sleep2011

    • Author(s)
      Yoshihiro Urade
    • Organizer
      Worldsleep 2011
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Key roles of the histaminergic system for the somnogenic effect of prostaglandin D2 and adenosine2011

    • Author(s)
      Zhi-Li Huang, Wei-Min Qu, Yoshihiro Urade, Osamu Hayaishi
    • Organizer
      Worldsleep 2011
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of adenosine A2A receptors in the neucleus accumbens for sleep-wake regulation2011

    • Author(s)
      Michael Lazarus, Yoshihiro Urade, Osamu Hayaishi
    • Organizer
      Worldsleep 2011
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] A novel SOX5 splicing isoform expressed in mouse brain during sleep2011

    • Author(s)
      Nanae Nagata, Kaori Kashiwagi, Toshiyoshi Yamamoto, Elizabeth P. Ko-Mitamura, Michael Lazarus, Zhi-Li Hunag, Ko Fujmori, Yoshihiro Urade
    • Organizer
      Worldsleep 2011
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Function of a novel SOX5 splicing isoform expressed in mouse brain during sleep2010

    • Author(s)
      Nanae Nagata, Kaori Kashiwagi, Toshiyoshi Yamamoto, et al.
    • Organizer
      Neuro2010
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] 2010)睡眠覚醒調節の分子機構2010

    • Author(s)
      裏出良博
    • Organizer
      第52回日本小児神経学会総会
    • Place of Presentation
      福岡
    • Year and Date
      2010-05-20
    • Related Report
      2012 Final Research Report
  • [Presentation] Recent progress on sleep-wake promotiong substances: Prostaglandin D . and adenosine2010

    • Author(s)
      Yoshihiro Urade, Huang Zhi-Li
    • Organizer
      Turkish-Japanese Sleep Forum
    • Place of Presentation
      Izmir, Turkey
    • Year and Date
      2010-05-04
    • Related Report
      2012 Final Research Report
  • [Presentation] Roles of histaminergic systems in sleep-wake regulation2010

    • Author(s)
      Zhi-Li Huang, Wei-Min Qu, Yoshihiro Urade
    • Organizer
      2010 Beijing international sleep medicine forum
    • Place of Presentation
      Beijing, China
    • Year and Date
      2010-04-18
    • Related Report
      2012 Final Research Report
  • [Presentation] 睡眠時に脳内で発 現する新規SOX5 スプライシングアイソ フォームの機能解析2010

    • Author(s)
      永田奈々恵、柏木香保里、山本利義、岡 崎一生、三田村エリザベス、Wei-Min Qu, Michael Lazarus, Zhi-Li Huang, 藤森 功、裏出良博
    • Organizer
      第33回日本神経 科学大会・第53回日本神経化学会大 会・第20回日本神経回路学会大会合同 大会
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.obi-dept2.com/

    • Related Report
      2012 Final Research Report

URL: 

Published: 2010-08-23   Modified: 2019-07-29  

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