| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Research Abstract |
We developed a micro-endoscope system which enables in vivo Ca imaging of neural circuits located deep brain area at cellular resolution in head-restricted non-anesthetized mice. We applied this system to the analysis of the cerebellar granule cell networks. We used mutants in which Ca sensor protein (GCaMP2) is expressed only in the cerebellar granule cell layers (Knopfel et al). First, we explored the role of synaptic transmission from the cerebellar granule cells in reflexive eye movements, by using a mutant in which synaptic transmission from cerebellar granule cells can be reversibly blocked in Dox dependent manner(Wada et al, 2014). Next, we conducted in vivo Ca imaging experiments in the cerebellar flocculus, which is known to contribute to the control of reflexive eye movement, and could visualize Ca response of cerebellar granule cells of the flocculus in vivo with physiological stimuli.
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