In vivo analysis of cerebellar granular cell networks using a micro-endoscope
| Project/Area Number |
22300136
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fusional brain recording science
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| Research Institution | Osaka Bioscience Institute |
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Principal Investigator |
FUNABIKI Kazuo 公益財団法人大阪バイオサイエンス研究所, システムズ生物学, 研究副部長 (00301234)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
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| Keywords | 小脳 / 顆粒細胞 / 顕微内視鏡 / 情報処理 / 学習理論 / 運動学習 / in vivo imaging / 小脳顆粒細胞 / 神経計算機構 / Ca イメージング / Caイメージング |
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| Research Abstract |
We developed a micro-endoscope system which enables in vivo Ca imaging of neural circuits located deep brain area at cellular resolution in head-restricted non-anesthetized mice. We applied this system to the analysis of the cerebellar granule cell networks. We used mutants in which Ca sensor protein (GCaMP2) is expressed only in the cerebellar granule cell layers (Knopfel et al). First, we explored the role of synaptic transmission from the cerebellar granule cells in reflexive eye movements, by using a mutant in which synaptic transmission from cerebellar granule cells can be reversibly blocked in Dox dependent manner(Wada et al, 2014). Next, we conducted in vivo Ca imaging experiments in the cerebellar flocculus, which is known to contribute to the control of reflexive eye movement, and could visualize Ca response of cerebellar granule cells of the flocculus in vivo with physiological stimuli.
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Report
(5 results)
Research Products
(31 results)
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[Journal Article] Protein kinase G dynamically modulates TASK1-mediated leak K+ currents in cholinergic neurons of the basal forebrain2010
Author(s)
Toyoda H, Saito M, Okazawa M, Hirao K, Sato H, Abe H, Takada K, Funabiki K, Takada M, Kaneko T, Kang Y
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Journal Title
J Neurosci
Volume: 30(16)
Pages: 5677-89
Related Report
Peer Reviewed
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[Journal Article] TRPM1 is a component of the retinal ON bipolar cell transduction channel in the mGluR6 cascade2010
Author(s)
Koike C, Obara T, Uriu Y, Numata T, Sanuki R, Miyata K, Koyasu T, Ueno S, Funabiki K, Tani A, Ueda H, Kondo M, Mori Y, Tachibana M, Furukawa T
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Journal Title
Proc Natl Acad Sci U S A
Volume: 107(1)
Pages: 332-7
Related Report
Peer Reviewed
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[Journal Article] Protein kinase G dynamically modulates TASK1-mediated leak K+currents in cholinergic neurons of the basal forebrain.2010
Author(s)
Toyoda H, Saito M, Okazawa M, Hirao K, Sato H, Abe H, Takada K, Funabiki K, Takada M, Kaneko T, Kang Y.
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Journal Title
J Neurosci.
Volume: 30
Pages: 5677-5689
Related Report
Peer Reviewed
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