Knock-out rat production and organ regeneration using embryonic stem cells
| Project/Area Number |
22300147
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
HIRABAYASHI Masumi 生理学研究所, 行動・代謝分子解析センター, 准教授 (20353435)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥13,390,000 (Direct Cost: ¥10,300,000、Indirect Cost: ¥3,090,000)
Fiscal Year 2012: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2011: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
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| Keywords | 胚性幹細胞 / キメララット / ランダムインテグレーション / ジーンターゲッティング / 臓器欠損 / 4倍体補完 / ノックインラット / ノックアウトラット / 遺伝子ターゲッティング / Pdx1遺伝子 / 相同組換え / エレクトロポレーション / 膵臓欠損 / ラット / トランスジェニック / FGFレセプターインヒビター / MEK活性化インヒビター / GSK3インヒビター / Kusabira-Orange遺伝子 / キメラ |
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| Research Abstract |
The ultimate objective of the present study is to produce gene-modified rats useful for regenerative medical research using pluripotent embryonic stem cells (ESCs) and advanced reproductive technologies (ART). Rats expressing the kusabira-orange gene, as well as rats expressing the tdTomato gene knock-in at Rosa26 locus, were successfully produced in preliminary trials. Rats lacking Pdx1 gene were also produced by a similar ART protocol with ESCs (Pdx1-deficient mice have no pancreas), but these rats did not show any phenotypic loss of the pancreas. However, functional rat pancreas was successfully regenerated in adult mice by injecting rat wild-type pluripotent stem cells (PSCs) into Pdx1/KO mouse blastocysts.
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Report
(4 results)
Research Products
(50 results)
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[Patent(Industrial Property Rights)] Method for producing heterogenous embryonic chimeric animal using a stem cell2011
Inventor(s)
Nakauchi H, Kobayashi T, Yamaguchi T, Hamanaka S, Hirabayashi M, Kato M
Industrial Property Rights Holder
Nakauchi H, Kobayashi T, Yamaguchi T, Hamanaka S, Hirabayashi M, Kato M
Filing Date
2011-11-17
Related Report
Overseas
