Mitochondria Treatment by Antioxidant with Multi-enzyme Activity

• Project/Area Number: 22300166
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: KAWAKAMI Hiroyoshi 首都大学東京, 都市環境科学研究科, 教授 (10221897)
• Co-Investigator(Kenkyū-buntansha): ASAYAMA Shoichiro 首都大学東京, 都市環境科学研究科, 准教授 (90315755) ; SHIMIZU Takahiko 千葉大学, 医学研究科, 客員准教授 (40301791)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2012: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 酵素 / 抗酸化 / キャリア / ミトコンドリア

Research Abstract

In this research, we have prepared the Mn-porphyrin-dimer-loaded liposomes for the rescue of H2O2-damaged cells. Furthermore, the antioxidative effect of the Mn-porphyrin dimer on the SOD-deficient cells and mice was examined. Moreover, we have synthesized a new Mn-porphyrin dimer to switch the enzyme activity by biological stimuli.

Research Products

• [Journal Article] Pharmaceutical Effect of Manganese Porphyrins on Manganese Superoxide Dismutase Deficient Mice (2012)
  • Author(s): N. Hayakawa, S. Asayama, Y. Noda, ShimizuT. ShimizuT., H. Kawakami
  • Journal Title: Mol. Pharmaceutics Volume: 9 Issue: 10 Pages: 2956-2959
  • DOI: 10.1021/mp300147v
  • Peer Reviewed
• [Journal Article] Up-regulation of Gene Expression by Transfection to Hepetocyte Spheroids (2012)
  • Author(s): K. Hazama, S. Asayama, H. Kawakami
  • Journal Title: Mol. Pharmaceutics Volume: 9 Pages: 3602-3605
• [Journal Article] New water-soluble Mn-porphyrin with catalytic activity for superoxide dismutation and peroxynitrite decomposition (2011)
  • Author(s): S. Asayama , T. Nakajima, H. Kawakami
  • Journal Title: Metallomics Volume: 3 Issue: 7 Pages: 744-748
  • DOI: 10.1039/c1mt00005e
• [Journal Article] Highly Amphiphilic Manganese Porphyrin for the Mitochondrial Targeting Antioxidant (2010)
  • Author(s): H.Kawakami, et al.
  • Journal Title: The Journal of Biochemistry Volume: 147 Pages: 153-156
  • NAID: 10027873554
  • Peer Reviewed
• [Journal Article] Design of lipoprotein-adsorbed liposomes retaining Mn-porphyrins for SOD mimic delivery to brains (2010)
  • Author(s): H.Kawakami, et al.
  • Journal Title: Desalination and Water Treatment Volume: 17 Pages: 31-36
  • Peer Reviewed
• [Journal Article] pH-Sensitive liposome retaining Mn-porphyrin as superoxide dismutase mimic for antioxidant delivery (2010)
  • Author(s): H.Kawakami, et al.
  • Journal Title: Porphyrins Volume: 19 Pages: 1-8
  • Peer Reviewed
• [Presentation] 人工カタラーゼとしてのイミダゾール基含有高分子/Mn-ポルフィリン複合体の分子設計 (2013)
  • Author(s): 窪田陸,朝山章一郎,川上浩良
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2013-03-29
• [Presentation] Mn-SOD欠損細胞及びマウスに対するMnポルフィリンダイマー含有リポソームの抗酸化活性評価 (2012)
  • Author(s): 米田祥浩, 山口翔平,清水孝彦, 朝山章一郎, 川上浩良
  • Organizer: 第61回高分子学会年次大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2012-05-31
• [Presentation] 金属キレート能を有する新規Mnポルフィリン錯体の合成とその抗酸化活性評価 (2012)
  • Author(s): 山口翔平
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学
  • Year and Date: 2012-03-30
• [Presentation] 金属キレート能を有する新規 Mn ポルフィリンダイマーの合成とその酵素活性評価 (2012)
  • Author(s): 山口翔平
  • Organizer: 第3回メタロミクス研究フォーラム
  • Place of Presentation: 昭和薬科大学
• [Presentation] Mnポルフィリンダイマーを用いたSOD欠損細胞に対する抗酸化評価 (2011)
  • Author(s): 窪田陸,田端久志, 清水孝彦(都健康長寿医療センター研),朝山章一郎,川上浩良
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: 静岡県立大学・静岡県コンベンションアーツセンター・ツインメッセ静岡
• [Presentation] Superoxide Dismutase Mimic-Loaded Liposome (2010)
  • Author(s): Hiroyoshi Kawakami
  • Organizer: International Liposome Research Days & Lipids, Liposomes & Membrane Biophysics
  • Place of Presentation: Toronto(Canada)
  • Year and Date: 2010-08-06