| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
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| Research Abstract |
The object of the present study was to investigate the cause which the onset of inborn error of metabolism is suppressed, when senescence accelerated mouse-prone 6 and 8 (SAMP6 and SAMP8) were raised on non-digestible oligosaccharide (FOS) and dietary fiber (GM) diets for long period. The composition of diet fed changes intestinal microflora and is transformed the activity of metabolism. As a result, the products of intestinal microbes are changed quantitatively and qualitatively and the existence of host is affected. To clarify the interaction between the change of intestinal microflora and the vital activity of host via intestinal by FOS and GM feeding, the variation of biological markers in concerned with oxidative stress, immune function, and bone metabolism was investigated. In 2012 and 2013, SAMP6 (n=45) with three groups (n=15 each group) and SAMP8 (n=45) with three groups (n=15 each group) were raised on control diet (CONT), FOS diet and GM diet for 32 weeks or 43 weeks, and th
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e biological markers were determined. In SAMP6 fed FOS diet and GM diet, intestinal microbes wereimproved and the bone metabolism became a very excellent. In especially, Bifidobacterium increased markedly and the activity of β-glucronidase and β-galactosidase decreased in FOS group, while in GM group Clostridium increased and the activity of β-glucronidase and β-galactosidase increased. However, the effect for RANKL and 1CTP was different from between FOS group and GM group. These results demonstrate that the function mechanism of FOS and GM are different between FOS and GM. SAMP8 fed FOS diet and GM diet was determined memory and learning disorder and oxidative markers such as BAP test, d-ROMs test, 8-OHdG and isopratan. In FOS group and GM group, intestinal microbes were improved and the senescence of brain was delayed. In addition, anti-oxidative function increased in comparison with that of control group, and inflammatory cytokines were suppressed. In 2013, in focus to brain function, SAMP8 was raised on CONT diet and FOS diet, and SAMR (normal senescence) was raised on CONT diet for 40 weeks to investigate the interaction among the memory and learning disorder, intestinal microbes and brain functions (amyloid-β, catecholamine, brain histochemistry, cytokines, oxidative stress marker s and et al.). The accumulation of amyloid-β in brain was suppressed by FOS ingestion, but the concentration of catecholamine was not changed. Inflammatory cytokineswere decreased by FOS ingestion, and anti-inflammatory cytokines were increased. The oxidative stress markers such as Bap test, d-Roms test, 8-OHdG and isoprastan were improved by FOS ingestion. These results demonstrate that long-term intake of FOS and GM improves intestinal microbes and also suppress or delay the learning and memory disorder and the aging in SAMP8. Thus, the suppression of oxidative stress and deposition of amyloid-β through intestinal microflora is concerned with senescenceand brain function. Less
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