Effects of nuclear receptor-activating compounds produced By microalgae on aquatic animal

• Project/Area Number: 22310020
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental impact assessment/Environmental policy
• Research Institution: University of Tsukuba
• Principal Investigator: KAYA Kunimitsu 筑波大学, 生命環境系, 教授 (40124341)
• Co-Investigator(Kenkyū-buntansha): SHIRAISHI Fujio 国立環境研究所, 環境リスク研究センター, フェロー (30113476) ; SANO Toharu 国立環境研究所, 環境リスク研究センター, 主任研究員 (10178808)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2010: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: 7-hydroxy retinoic acid / RAR agonistic activity / Cyanobacteria / Teratogen / 7-Hydroxy retinoic acid / cis-trans isomerization / keto-enol tautomerism / teratogenic test / all-trans retinoic acid / zebrafish / cyanobacteria / photostability / medaka ER / yeast-two-hybrid assay / E2 / Planktothrix agardhii / warerblooms / Cylindrospermopsis raciborskii / nuclear receptors / RAR / Two-hybrid assay / Microcysits / spirulina / ATLA / UV-A

Research Abstract

A novel analogue of retinoic acid, 7-hydroxy retinoic acid, was found in waterbloom-forming cyanobacteria. The analogue had an agonistic activity against retinoic acid receptor (RAR) as a kind of nuclear receptors. The novel analogue was isolated from mass-cultured Spirulina sp.(TU-3).The analogue was found in all-tested strains of cyanobacteria. The analogue exist a mixture of four isomers by cis-trans isomerization including keto-enol tautomerism. The agonistic activity of the analogue against RAR was a half of all-trans retinoic acid (ATRA). The analogue was far-stable under UV-A compared with ATRA. The stability of 7-hydroxy retinoic acid under UV-A suggest that amphibians and fishes living in waterblooms occurring waters may be exposure, and give teratogenic effects.

Research Products

• [Journal Article] A novel retinoic acid analogue, 7-hydroxy retinoic acid, isolated from cyanobacteria (2011)
  • Author(s): Kaya K, Shiraishi F., Uchida H and Sano T
  • Journal Title: Biochim. Biophys. Acta Volume: 1810 Issue: 4 Pages: 414-419
  • DOI: 10.1016/j.bbagen.2010.11.009
  • Peer Reviewed
• [Journal Article] A novel retinoic acid analogue, 7-hydroxy retinoic acid isolated from cyanobacteria (2011)
  • Author(s): Kaya, K, Shiraishi, F, Uchida H, Sano, T.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1810 Pages: 414-419
  • Peer Reviewed
• [Presentation] Is 7-hydroxy retinoic acid of cyanobacteria an aquatic ecotoxin? (2013)
  • Author(s): Kaya, Kunimitsu
  • Organizer: 9th International Conference on Toxic Cyanobacteria
  • Place of Presentation: Johannesburg, South Africa
• [Presentation] Is 7-hydroxy retinoic acid of cyanobacteria an aquatic ecotoxin? (2012)
  • Author(s): Kaya K, Shiraishi F, and Sano T
  • Organizer: 9^<th> International Conference on To xi c Cyanobacteria.
  • Place of Presentation: Johannesburg, South Africa
  • Year and Date: 2012-08-12
• [Presentation] A photostable retinoic acid analogue, 7-hydrioxy retinoic acid isolated from Spirulina and other cyanobacteria (2012)
  • Author(s): Kaya K, ShiraishiF, Sano T
  • Organizer: 8^<th> Asia-Pacific Conference on Algal Biotechnology
  • Place of Presentation: Adelaide, Australia
  • Year and Date: 2012-07-11
• [Presentation] A photostable retinoic acid analogue, 7-hydroxy retinoic acid, isolated from Spirulina and other cyanobacteria (2012)
  • Author(s): Kaya, Kunimitsu
  • Organizer: 8th Asia-Pacific conference on Algal Biotechnology
  • Place of Presentation: Adelaide, Australia