Molecular genetics of cell niche and radiation sensitivity in tissue construction of Oryzias latipes
| Project/Area Number |
22310035
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TODO Takeshi 大阪大学, 大学院・医学系研究科, 教授 (90163948)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2012: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
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| Keywords | 細胞微小環境 / 組織幹細胞 / メダカ / 放射線 / 組織特異的発現 / 細胞周期チェックポイント増殖抑制 / 損傷応答 / 細胞周期チェックポイント / 増殖抑制 / 増殖制御 |
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| Research Abstract |
To develop the system to study DNA damage response in tissue stem cell, in this study we have established several mutants in two different pathways in Medaka. One is the signal transduction pathway, responsibility to DNA damage. ATM, ATR and DNA-PKcs are member of PI3 kinase family. These gene products play an important role on the initial step of damage response. We have already established ATM and ATR mutants. In this study we have identified DNA-PKcs mutant. We have also obtained p53 mutant, which also play essential role on damage response. Our goal is to analyze the damage response in Medaka using these mutants carrying a single or a multiple mutation. Another is the signaling pathway which regulates cell growth in tissue. The Wnt signaling pathways are a group of signal transduction pathways, which leads to regulation of transcription and cell growth. These signaling pathways are activated by the binding of a Wnt ligand to a Frizzled family receptor. To establish system to regulate cell growth, we haveestablished Frizzled mutants. Establishment of these mutants enables us to study the cross-talk between damage response and growth signaling.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] ATF6α/β-mediated adjustment of ER chaperone levels is essential for development of the notochord in medaka fish.2013
Author(s)
Ishikawa T, Okada T, Ishikawa-Fujiwara T, Todo T, Kamei Y, Shigenobu S, Tanaka M, Saito TL, Yoshimura J, Morishita S, Toyoda A, Sakaki Y, Taniguchi Y, Takeda S, Mori K.
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Journal Title
Mol Biol Cell
Volume: 24(9)
Issue: 9
Pages: 1387-1395
DOI
Related Report
Peer Reviewed
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[Journal Article] Characterization of two members of the cryptochrome/photolyase family from Ostreococcus tauri provides insights into the origin and evolution of cryptochromes2010
Author(s)
Heijde M, Zabulon G, Corellou F, Ishikawa T, Brazard J, Usman A, Sanchez F, Plaza P, Martin M, Falciatore A, Todo T, Bouget FY, Bowler C
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Journal Title
Plant Cell Environ
Volume: 33(10)
Issue: 10
Pages: 1614-26
DOI
Related Report
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[Journal Article] High-resolution melting curve analysis for rapid detection of mutations in a Medaka TILLING library2010
Author(s)
Ishikawa T, Kamei Y, Otozai S, Kim J, Sato A, Kuwahara Y, Tanaka M, Deguchi T, Inohara H, Tsujimura T, Todo T.
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Journal Title
BMC Mol Biol
Volume: 11
Pages: 70-70
Related Report
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[Presentation] ATF6 plays an essential role in induction of ER chaperones required for notochord formation2012
Author(s)
Tokiro Ishikawa, Tetsuya Okada, Tomoko Ishikawa -Fujiwara, Takeshi Todo, Yasuhiro Kamei, Shuji Shigenobu, Minoru Tanaka, Taro L. Saito, Jun Yoshimura, Shinichi Morishita, Atsushi Toyoda, Yoshiyuki Sakaki, Yoshihito Taniguchi, Shunichi Takeda, Kazutoshi Mo
Organizer
18th Japanese Medaka and Zebrafish Meeting
Place of Presentation
Kyoto
Year and Date
2012-09-22
Related Report
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[Presentation] Molecular characterization of marine rhythms.2012
Author(s)
Tessmar-Raible, K., Zantke, J., Kuegler, P., Oliveri, P., Fortunato, A.E., Falciatore, A., Ribera d'Alcala, M., Buia, M.C., Iacono, B., Zikihara, K.,Ishikawa, T
Organizer
12thHFSP Awadees Meeting Daegu
Place of Presentation
Republic of Korea
Year and Date
2012-07-04
Related Report
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