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Development of environmental technology based on molecular basis of microbial one-carbon metabolism

Research Project

Project/Area Number 22310046
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental technology/Environmental materials
Research InstitutionKyoto University

Principal Investigator

YURIMOTO Hiroya  京都大学, 大学院・農学研究科, 准教授 (00283648)

Co-Investigator(Kenkyū-buntansha) SAKAI Yasuyoshi  京都大学, 大学院・農学研究科, 教授 (60202082)
OKU Masahide  京都大学, 大学院・農学研究科, 助教 (10511230)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywordsメタン / メタノール / ホルムアルデヒド / C1微生物 / メタン資化性細菌 / メタノール資化性酵母 / メタノール資化性細菌 / 代謝制御
Research Abstract

In order to develop environmental technology regarding C1 compounds, such as methane, methanol and formaldehyde, we have studied molecular basis of C1 metabolism and its application. For example, we successfully expressed bacterial formaldehyde-fixing enzyme, Hps-Phi, in methanotrophs, methylotrophs, and plants. Furthermore, we revealed molecular functions of novel factors involved in yeast formaldehyde-responsiblegene expression.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (39 results)

All 2013 2012 2011 2010

All Journal Article (29 results) (of which Peer Reviewed: 24 results) Presentation (9 results) (of which Invited: 1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Expression level of methanol-inducible peroxisomal proteins and peroxisome morphology are affected by oxygen conditions and mitochondrial respiratory pathway function in the methylotrophic yeastCandida boidinii.2013

    • Author(s)
      S. Fujimura, H. Yurimoto, S. Kurimoto, Y.Matsufuji, T. Ito, T. Hayakawa, N. Tomizuka, Y. Sakai, and T. Nakagawa
    • Journal Title

      FEMS Yeast Res

      Volume: 13 Issue: 4 Pages: 359-366

    • DOI

      10.1111/1567-1364.12040

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Roseomonas musae sp. nov., a new bacterium isolated from a banana phyllosphere.2013

    • Author(s)
      P. Nutaratat, N. Srisuk, K. Duangmal, H. Yurimoto, Y. Sakai, Y. Muramatsu, and Y.Nakagawa
    • Journal Title

      Antonie van Leeuwenhoek

      Volume: 103 Issue: 3 Pages: 617-624

    • DOI

      10.1007/s10482-012-9845-5

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of the Candida boidinii MIG1and its role in regulation of methanol inducible gene expression2012

    • Author(s)
      Z. Zhai, H. Yurimoto, and Y. Sakai
    • Journal Title

      Yeast

      Volume: 29 Issue: 7 Pages: 293-301

    • DOI

      10.1002/yea.2909

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of Methanotrophs in the Phyllosphere2012

    • Author(s)
      H. Iguchi, I. Sato, M. Sakakibara, H. Yurimoto, and Y. Sakai
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 8 Pages: 1580-1583

    • DOI

      10.1271/bbb.120281

    • NAID

      10031066412

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Distribution of Pink-Pigmented Facultative Methylotrophs on Leaves of Vegetables2012

    • Author(s)
      M. Mizuno, H. Yurimoto, N. Yoshida, H. Iguchi, and Y. Sakai
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 3 Pages: 578-580

    • DOI

      10.1271/bbb.110737

    • NAID

      10030750921

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Msn5p Is Involved in Formaldehyde Resistance but Not in Oxidative Stress Response in the Methylotrophic Yeast Candida boidinii2012

    • Author(s)
      Z. Zhai, H. Yurimoto, and Y. Sakai
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 76 Issue: 2 Pages: 299-304

    • DOI

      10.1271/bbb.110679

    • NAID

      10030399766

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Stimulation of methanotrophic growth in co-cultures by cobalamin excreted by rhizobia.2011

    • Author(s)
      H. Iguchi, H. Yurimoto, and Y. Sakai
    • Journal Title

      Appl. Environ. Microbiol

      Volume: 77 Issue: 24 Pages: 8509-8515

    • DOI

      10.1128/aem.05834-11

    • NAID

      120003692388

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Yeast methylotrophy and autophagy in a methanol-oscillating environment on growing Arabidopsis thaliana leaves.2011

    • Author(s)
      K. Kawaguchi, H. Yurimoto, M. Oku, and Y. Sakai
    • Journal Title

      PLoS ONE

      Volume: 6 Issue: 9 Pages: e25257-e25257

    • DOI

      10.1371/journal.pone.0025257

    • NAID

      120003367881

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] C1 微生物複合生物系による省エネ型炭素固定技術2011

    • Author(s)
      阪井康能、由里本博也
    • Journal Title

      環境資源工学

      Volume: 58 Pages: 59-63

    • Related Report
      2012 Final Research Report
  • [Journal Article] Inhibition of surgical trauma-enhanced peritoneal dissemination of tumor cells by human catalase derivatives in mice2011

    • Author(s)
      C. Nishizaki, M. Nishikawa, T. Yata, T. Yamada, Y. Takahashi, M. Oku, H. Yurimoto, Y. Sakai,K. Nakanishi, and Y. Takakura
    • Journal Title

      Free Radic. Biol. Med

      Volume: 51 Issue: 3 Pages: 773-779

    • DOI

      10.1016/j.freeradbiomed.2011.05.025

    • NAID

      120003255726

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Yeast methylotrophy: metabolism, gene regulation, and peroxisome homeostasis2011

    • Author(s)
      H. Yurimoto, M. Oku, and Y. Sakai
    • Journal Title

      Int. J. Microbiol

      Volume: 2011 Pages: 101298-101298

    • DOI

      10.1155/2011/101298

    • NAID

      120003405543

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methylovulum miyakonense gen. nov., sp. Nov., a novel type I methanotroph from a forest soil.2011

    • Author(s)
      H. Iguchi, H. Yurimoto, and Y. Sakai
    • Journal Title

      Int. J. Syst. Evol. Microbiol.

      Volume: 61 Issue: 4 Pages: 810-815

    • DOI

      10.1099/ijs.0.019604-0

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 光合成経路と相性のよいホルムアルデヒド固定反応2011

    • Author(s)
      由里本博也、阪 井 康 能
    • Journal Title

      バイオサイエンスとインダストリー

      Volume: 69 Pages: 46-48

    • NAID

      10027617195

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
  • [Journal Article] C1微生物複合生物系による省エネ型炭素固定技術2011

    • Author(s)
      阪井康能、由里本博也
    • Journal Title

      環境資源工学

      Volume: 58 Pages: 59-63

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Methylovulum miyakonense gen.nov., sp.nov., a novel type I methanotroph from a forest soil.2011

    • Author(s)
      H.Iguchi, H.Yurimoto, Y.Sakai.
    • Journal Title

      Int.J.Syst.Evol.Microbiol.

      Volume: 61 Pages: 810-815

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] C1微生物と植物の相互作用と炭素循環 ‐代謝生化学・分子細胞生物学からの理解‐2010

    • Author(s)
      由里本博也、阪井康能
    • Journal Title

      植物の生長調節

      Volume: 45 Pages: 125-131

    • Related Report
      2012 Final Research Report
  • [Journal Article] Crystal structure of 3-hexulose- 6-phosphate synthase, a member of the orotidine 5'-monophosphate decarboxylase suprafamily2010

    • Author(s)
      I. Orita, A. Kita, H. Yurimoto, N. Kato, Y. Sakai, and K. Miki
    • Journal Title

      Proteins

      Volume: 78 Issue: 16 Pages: 3488-3492

    • DOI

      10.1002/prot.22860

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Soluble and particulate methane monooxygenase gene clusters in the type I methanotrophMethylovulum miyakonense HT12.2010

    • Author(s)
      H. Iguchi, H. Yurimoto, and Y. Sakai
    • Journal Title

      FEMS Microbiol. Lett.

      Volume: 312 Issue: 1 Pages: 71-76

    • DOI

      10.1111/j.1574-6968.2010.02101.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of an HPS/PHI fusion enzyme from Mycobacterium gastri in chloroplasts of geranium enhances its ability to assimilate and phytoremediate formaldehyde2010

    • Author(s)
      Z. Song, I. Orita, F. Yin, H. Yurimoto, N. Kato,Y. Sakai, K. Izui, K. Li, and L. Chen
    • Journal Title

      phytoremediate formaldehyde.

      Volume: 32 Issue: 10 Pages: 1541-1548

    • DOI

      10.1007/s10529-010-0324-7

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Peroxisomal Catalase Gene in the Methylotrophic Yeast Pichia methanolica2010

    • Author(s)
      T. Nakagawa, K. Yoshida, A. Takeuchi, T. Ito, S. Fujimura, Y. Matsufujii, N. Tomizuka, H. Yurimoto, Y. Sakai, and T. Hayakawa
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74 Issue: 8 Pages: 1733-1735

    • DOI

      10.1271/bbb.100329

    • NAID

      10027558835

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Characterization of Two Genes with High Similarity to the Dihydroxyacetone Synthase Gene in the Methylotrophic Yeast Pichia methanolica2010

    • Author(s)
      T. Nakagawa, S. Fujimura, T. Ito, Y. Matsufujii,S. Ozawa, T. Miyaji, J. Nakagawa, N. Tomizuka, H. Yurimoto, Y. Sakai,and T. Hayakawa
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74 Issue: 7 Pages: 1491-1493

    • DOI

      10.1271/bbb.100153

    • NAID

      10027557611

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Trm2p-dependent derepression is essential for methanol-specific gene activation in the methylotrophic yeast Candida boidinii.2010

    • Author(s)
      Y. Sasano, H. Yurimoto, M. Kuriyama, and Y. Sakai
    • Journal Title

      FEMS Yeast Res

      Volume: 10 Pages: 535-544

    • DOI

      10.1111/j.1567-1364.2010.00640.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Trm2p-dependent derepression is essential for methanol-specific gene activation in the methylotrophic yeast Candida boidinii2010

    • Author(s)
      Y.Sasano, H.Yurimoto, M.Kuriyama, Y.Sakai.
    • Journal Title

      FEMS Yeast Res.

      Volume: 10 Pages: 535-544

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular characterization of two genes with high similarity to the dihydroxyacetone synthase gene in the methylotrophic yeast Pichia methanolica.2010

    • Author(s)
      T.Nakagawa, S.Fujimura, T.Ito, Y.Matsufujii, S.Ozawa, T.Miyaji, J.Nakagawa, N.Tomizuka, H.Yurimoto, Y.Sakai, T.Hayakawa.
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 74 Pages: 1491-1493

    • NAID

      10027557611

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Peroxisomal catalase gene in the methylotrophic yeast Pichia methanolica.2010

    • Author(s)
      T.Nakagawa, K.Yoshida, A.Takeuchi, T.Ito, S.Fujimura, Y.Matsufujii, N.Tomizuka, H.Yurimoto, Y.Sakai, T.Hayakawa.
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 74 Pages: 1733-1735

    • NAID

      10027558835

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overexpression of an HPS/PHI fusion enzyme, from Mycobacterium, gastri in, chloroplasts of geranium enhances its ability to assimilate and phytoremediate formaldehyde.2010

    • Author(s)
      Z.Song, I.Orita, F.Yin, H.Yurimoto, N.Kato, Y.Sakai, K.Izui, K.Li, L.Chen.
    • Journal Title

      Biotechnol.Lett.

      Volume: 32 Pages: 1541-1548

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Soluble and particulate methane monooxygenase gene clusters in the type I methanotroph Methylovulum miyakonense HT12.2010

    • Author(s)
      H.Iguchi, H.Yurimoto, Y.Sakai.
    • Journal Title

      FEMS Microbiol.Lett.

      Volume: 312 Pages: 71-76

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of 3-hexulose-6-phosphate synthase, a member of the orotidine 5'-monophosphate decarboxylase suprafamily.2010

    • Author(s)
      I.Orita, A.Kita, H.Yurimoto, N.Kato, Y.Sakai, K.Miki.
    • Journal Title

      Proteins

      Volume: 78 Pages: 3488-3492

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] C1微生物と植物の相互作用と炭素循環-代謝生化学・分子細胞生物学からの理解-2010

    • Author(s)
      由里本博也、阪井康能
    • Journal Title

      植物の生長調節

      Volume: 45 Pages: 125-131

    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of environmental technology based on symbiotic interactions of C1-microorganisms and plants.2012

    • Author(s)
      Yurimoto, H
    • Organizer
      The 6th JapanFinland Biotechnology Symposium
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2012 Final Research Report
  • [Presentation] Development of environmental technology based on symbiotic interactions of C1-microorganisms and plants2012

    • Author(s)
      H. Yurimoto, H. Iguchi, K. Kawaguchi, and Y. Sakai
    • Organizer
      The 6th Japan-Finland Biotechnology Symposium
    • Place of Presentation
      東北学院大学(仙台市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 植物から放出されるC1化合物と植物表層のC1微生物2011

    • Author(s)
      田里本博也、川口甲介、井口博之、阪井康能
    • Organizer
      第17回大気化学討論会
    • Place of Presentation
      京都大学(招待講演)
    • Year and Date
      2011-10-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] Metabolic flux regulates methanol-inducible gene expression in the methylotrophic yeast Candida boidinii2011

    • Author(s)
      Yurimoto, H
    • Organizer
      The 25th International Conference on Yeast Genetics and Molecular Biology
    • Place of Presentation
      Olsztyn, Poland
    • Related Report
      2012 Final Research Report
  • [Presentation] Metabolic flux regulates methanol-inducible gene expression in the methylotrophic yeast Candida boidnii2011

    • Author(s)
      Hiroya Yurimoto, Toshiki Kosakai, Rie Sano, Yasuyoshi Sakai
    • Organizer
      TKe 25th International Conference on Yeast Genetics and Molecular Biology
    • Place of Presentation
      Olsztyn (ポーランド)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Biotechnological application of metabolic functions in C1-microorganisms.2010

    • Author(s)
      Yurimoto, H
    • Organizer
      The 5th Japan- Finland Biotechnology Symposium
    • Place of Presentation
      Turku, Finland
    • Year and Date
      2010-06-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Biotechnological application of metabolic functions in C_1-microorganisms2010

    • Author(s)
      Yurimoto, H.
    • Organizer
      The 5th Japan-Finland Biotechnology Symposium
    • Place of Presentation
      Turku (Finland)招待講演
    • Year and Date
      2010-06-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Gene organization and function of the RuMP pathway enzymes in methanotrophs2010

    • Author(s)
      Yurimoto, H
    • Organizer
      Gordon Research Conference on Molecula Basis of Microbial One-Carbon Metabolism.
    • Place of Presentation
      Lewiston, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Gene organization and function of the RuMP pathway enzymes in methanotrophs2010

    • Author(s)
      Yurimoto, H.
    • Organizer
      Gordon Research Conference on Molecular Basis of Microbioal One-Carbon Metabolism
    • Place of Presentation
      Lewiston, ME (USA)
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] メタン酸化菌に対して用いられるメタン酸化活性向上剤、及びその利用2010

    • Inventor(s)
      阪井康能、井口博之、由里本博也
    • Industrial Property Rights Holder
      京都大学
    • Industrial Property Number
      2010-183287
    • Filing Date
      2010-08-18
    • Related Report
      2012 Final Research Report 2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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