Development of near-field optical microscopy for hydrogen ion concentration and membrane potential using environmental sensitivity dye
| Project/Area Number |
22310078
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
UMEDA Norihiro 東京農工大学, 大学院・工学研究院, 教授 (60111803)
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| Co-Investigator(Kenkyū-buntansha) |
IWAMI Kentarou 東京農工大学, 大学院・工学研究院, 准教授 (80514710)
OHTA Yoshihiro 東京農工大学, 大学院・工学研究院, 准教授 (10223843)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2010: ¥13,650,000 (Direct Cost: ¥10,500,000、Indirect Cost: ¥3,150,000)
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| Keywords | 近接場光学顕微鏡 / 蛍光感受性色素 / ミトコンドリア / pH / ネクローシス / 多光子吸収 / プロトンポンプ / 蛍光色素 / ｐH / フェムト秒レーザー |
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| Research Abstract |
In this study, a dual-wavelength pH-sensitive dye was excited by two-photon absorption initiated using afemtosecond pulse laser. In addition, fluorescence from the dye was directly collected from the fluorescent point using the collection-mode probe of a scanning near-field optical microscope. By thisproposed method, a pH calibration curve was obtained from the fluorescent intensity ratio of the dyesolution, and temporal pH variations with 0.1 s time resolution following the addition of acid wereobserved. Moreover, mitochondrial activity on the basis of the pH changes was successfully observed in three different mitochondrial densities.
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Report
(4 results)
Research Products
(34 results)
