Development of vitreous ice technique and correlative microscopy to observe biological specimens at a molecular resolution
| Project/Area Number |
22310079
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Osaka University |
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Principal Investigator |
IWASAKI Kenji 大阪大学, 蛋白質研究所, 准教授 (20342751)
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| Co-Investigator(Renkei-kenkyūsha) |
TAKAGI Junichi 大阪大学, 蛋白質研究所, 教授 (90212000)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2012: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2011: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
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| Keywords | 電子線トモグラフィー / 相関顕微鏡法 / CEMOVIS / 電子顕微鏡 / 相関顕微鏡 / クライオ電子顕微鏡 |
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| Research Abstract |
We attempted to gage the tilt angle using a capacitive liquid-based inclinometer when the projection images for tomographic reconstructionwere acquired. As a result, the tile angle measured has the same accuracy as those obtained by the marker-based alignment, which is most popular alignment method in tomography. When the enough fiducial markers are not available in the projection images, measuring the tilt angle will be the useful method to obtain the better tomogram.
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Report
(4 results)
Research Products
(42 results)
