Development of a micro-device for manipulation and analysis of single genomic DNA molecules toward single cell analysis
Project/Area Number |
22310080
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Microdevices/Nanodevices
|
Research Institution | The University of Tokyo |
Principal Investigator |
OANA Hidehiro 東京大学, 大学院・工学系研究科, 准教授 (20314172)
|
Co-Investigator(Renkei-kenkyūsha) |
WASHIZU Masao 東京大学, 大学院・工学系研究科, 教授 (10201162)
GEL Murat 東京大学, 大学院・工学系研究科, 助教 (90456153)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
|
Keywords | DNA / ゲノム / ナノバイオ / マイクロ / ナノデバイス / バイオテクノロジー / マイクロ・ナノデバイス / マイクロ・ナノテバイス |
Research Abstract |
We developed a new methodology for investigation ofindividual, intact chromatin fibers isolated from individual cells, utilizinga custom-designed microfluidic device and a novel technique for handlingindividual chromatin fibers by fluorescence microscopy. The method developedhere has potential application for direct investigation of the epigenetic profileof intact chromatin fibers at individual cell level.
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Report
(4 results)
Research Products
(18 results)