Development of a micro-device for manipulation and analysis of single genomic DNA molecules toward single cell analysis

• Project/Area Number: 22310080
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Microdevices/Nanodevices
• Research Institution: The University of Tokyo
• Principal Investigator: OANA Hidehiro 東京大学, 大学院・工学系研究科, 准教授 (20314172)
• Co-Investigator(Renkei-kenkyūsha): WASHIZU Masao 東京大学, 大学院・工学系研究科, 教授 (10201162) ; GEL Murat 東京大学, 大学院・工学系研究科, 助教 (90456153)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2012: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: DNA / ゲノム / ナノバイオ / マイクロ / ナノデバイス / バイオテクノロジー / マイクロ・ナノデバイス / マイクロ・ナノテバイス

Research Abstract

We developed a new methodology for investigation ofindividual, intact chromatin fibers isolated from individual cells, utilizinga custom-designed microfluidic device and a novel technique for handlingindividual chromatin fibers by fluorescence microscopy. The method developedhere has potential application for direct investigation of the epigenetic profileof intact chromatin fibers at individual cell level.

Research Products

• [Journal Article] Direct Formation of Giant Unilamellar Vesicles from Microparticles of Polyion Complexes and Investigation of Their Properties Using a Microfluidic Chamber (2013)
  • Author(s): Hidehiro Oana
  • Journal Title: Soft Matter Volume: in press Issue: 22 Pages: 696-704
  • DOI: 10.1039/c3sm00089c
  • Peer Reviewed
• [Journal Article] Optical Sequence Probingwith the Homologous Recombination Protein RecA (2012)
  • Author(s): Yuji Kimura, Yuya Goto, Hidehiro Oana,Masao Washizu
  • Journal Title: Journal of Biotechnology Volume: 164 Pages: 254-259
  • URL: http://dx.doi.org/10.1016/j.jbiotec.2012.08.006
• [Journal Article] Optical Sequence Probing with the Homologous Recombination Protein RecA (2012)
  • Author(s): Yuji Kimura, Yuya Goto, Hidehiro Oana,Masao Washizu
  • Journal Title: Journal of Biotechnology Volume: 164, Pages: 254-259
  • URL: http://dx.doi.org/10.1016/j.jbiotec.2012.08.006
• [Journal Article] EXTENSION, IMMOBILIZATION AND CHEMICAL MODIFICATION OF DOUBLE-STRANDED DNA ON A SOLID SURFACE-TOWARD DIRECT SEQUENCING WITH MICROSCOPY- (2010)
  • Author(s): K.Nishikawa, et al.
  • Journal Title: Proc.of MicroTAS2010 Volume: 1 Pages: 82-84
• [Presentation] REAL-TIME FISH USING OPTICALLY-DRIVEN MICROSPHERES FUNCTIONALIZED BY THE HOMOLOGOUS RECOMBINATION PROTEIN, RecA (2012)
  • Author(s): Hidehiro Oana, Tatsuya Shino, Kaori Nishikawa, Masao Washizu
  • Organizer: 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences(MicroTAS 2012)
  • Place of Presentation: 宜野湾市
  • Year and Date: 2012-10-29
• [Presentation] 相同組換えタンパクを用いた特定塩基配列探索技術の開発 (2012)
  • Author(s): 小穴英廣,志野達弥,西川香里,鷲津正夫
  • Organizer: 第25回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 熊本市
  • Year and Date: 2012-05-18
• [Presentation] Formation of Polymer Vesicles from Microdroplets of Polyion Complex in a Microfluidic Chamber (2011)
  • Author(s): Hidehiro Oana, Mutsuki Morinaga,Akihiro Kishimura, Murat Gel, Kazunori Kataoka, and Masao Washizu
  • Organizer: MicRO Alliance Meeting 2011
  • Place of Presentation: アナーバー、アメリカ合衆国
  • Year and Date: 2011-10-09
• [Presentation] Formation of Polymer Vesicles from Microdroplets of Polyion Complex in a Microfluidic Chamber (2011)
  • Author(s): Hidehiro Oana, et al.
  • Organizer: MicRO Alliance Meeting 2011
  • Place of Presentation: アメリカ合衆国アナーバー
  • Year and Date: 2011-10-09
• [Presentation] Formation of Polymer Vesicles from Microdroplets of Polyion Complex and Examination of Their Physicochemical Properties in Microfluidic Chamber (2011)
  • Author(s): Hidehiro Oana, Mutsuki Morinaga,Akihiro Kishimura, Murat Gel, Kazunori Kataoka, and Masao Washizu
  • Organizer: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011)
  • Place of Presentation: シアトル、アメリカ合衆国
  • Year and Date: 2011-10-05
• [Presentation] Formation of Polymer Vesicles from Microdroplets of Polyion Complex and Examination of Their Physicochemical Properties in Microfluidic Chamber (2011)
  • Author(s): Hidehiro Oana, et al.
  • Organizer: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2011)
  • Place of Presentation: アメリカ合衆国シアトル
  • Year and Date: 2011-10-05
• [Presentation] EXTENSION, IMMOBILIZATION AND CHEMICAL MODIFICATION OF DOUBLE-STRANDED DNA ON A SOLID SURFACE -TOWARD DIRECT SEQUENCING WITH MICROSCOPY- (2010)
  • Author(s): K. Nishikawa, M. Kataoka, R. Nagata, A.Kitayama, R. Tero, M. Washizu and H. Oana:
  • Organizer: 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2010)
  • Place of Presentation: フローニンゲン、オランダ
  • Year and Date: 2010-10-04
• [Presentation] Folding Dynamics of Large DNA Molecules under Steady Strong Flow (2010)
  • Author(s): Hidehiro Oana, Takuya Saito, Takahiro Sakaue, Daiji Kaneko, Masao Washizu:
  • Organizer: The fifth Pacific Rim Conference on Rheology(PRCR5)
  • Place of Presentation: 札幌市
  • Year and Date: 2010-08-05
• [Presentation] Folding Dynamics of Large DNA Molecules under Steady Strong Flow (2010)
  • Author(s): H.Oana, et al.
  • Organizer: the fifth Pacific Rim Conference on Rheology (PRCRS)
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2010-08-05
• [Presentation] 基板上に伸長固定した DNA の塩基修飾反応とその検出 (2010)
  • Author(s): 西川香里, 片岡正典, 永田麗子, 喜多山篤, 手老龍吾, 鷲津正夫, 小穴英廣
  • Organizer: 第21回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 東京都文京区
  • Year and Date: 2010-06-10
• [Presentation] 基板上に伸長固定したDNAの塩基修飾反応とその検出 (2010)
  • Author(s): 西川香里, 他
  • Organizer: 第21回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-06-10
• [Presentation] 相同組換えタンパクを用いた特定塩基配列探索技術の開発
  • Author(s): 小穴英廣
  • Organizer: 第25回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 熊本県 熊本市 崇城大学
• [Presentation] REAL-TIME FISH USING OPTICALLY-DRIVEN MICROSPHERES FUNCTIONALIZED BY THE HOMOLOGOUS RECOMBINATION PROTEIN, RecA
  • Author(s): Hidehiro Oana
  • Organizer: 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences
  • Place of Presentation: 沖縄県 宜野湾市 沖縄コンベンションセンター
• [Remarks]
  • URL: http://www.bntl.t.u-tokyo.ac.jp