Microneedle skin vaccine

• Project/Area Number: 22310082
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Microdevices/Nanodevices
• Research Institution: Kyoto Pharmaceutical University
• Principal Investigator: TAKADA Kanji 京都薬科大学, 薬学部, 教授 (30102106)
• Co-Investigator(Renkei-kenkyūsha): ITO Yukako 京都薬科大学, 薬学部, 講師 (30278444)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000); Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: マイクロファブリケーション / マイクロナノデバイス / 感染症 / マイクロアレイ / 薬学 / 免疫学 / マイクロ・ナノデバイス

Research Abstract

Two- and three-layered dissolving microneedles was loaded with ovalbumin (OA) at the acral and second portion of the needles. As 3-layered MNs delivered anitigen to Langerhans cells that localize in the epiderm, stronger immunological reaction was induced. On the other hand, the target site of tetanus toxoid was revealed to be the Langerhans cells distributed mainly in the skin epiderm.

Research Products

• [Journal Article] High percutaneous bioavailability of granisetron by dissolving microneedles. (2012)
  • Author(s): Asuka Ikejiri, Yukako Ito and Kanji Takada
  • Journal Title: J. Drug. Deliv. Sci. Technol. Volume: 22 Pages: 500-504
  • Peer Reviewed
• [Journal Article] Transdermal Insulin application system with dissolving microneedles. (2012)
  • Author(s): Yukako Ito, Takuya Nakhigashi, NaokoYoshimoto, Yuriko Ueda, Noriyuki Hamasaki, and Kanji Takada
  • Journal Title: Diabetes. Technol. Ther. Volume: 14 Pages: 891-899
  • Peer Reviewed
• [Journal Article] Two-layered dissolving microneedles formulated with intermediate-acting insulin. (2012)
  • Author(s): Yukako Ito, Muneyuki Hirono, Keizo Fukushima, Nobuyuki Sugioka and Kanji Takada
  • Journal Title: Int. J. Pharm. Volume: 436 Pages: 387-393
  • Peer Reviewed
• [Journal Article] Two- and three-layered Dissolving Microneedles for Transcutaneous Delivery of Model. Vaccine Antigen in Rats. (2012)
  • Author(s): Asuka Ikejiri, Seishiro Naito, Yukako Ito and Kanji Takada
  • Journal Title: J. Biomaterials. Nanobiotech. Volume: 3 Pages: 325-334
  • Peer Reviewed
• [Journal Article] Antigen-loaded dissolving microneedle array as a novel tool for percutaneous vaccination. (2012)
  • Author(s): Seishiro Naito, Yukako Ito, Tomoko Kiyohara, Michiyo Kataoka, Masaki Ochiai and Kanji Takada
  • Journal Title: Vaccine Volume: 30 Pages: 1191-1197
  • Peer Reviewed
• [Journal Article] Effect of lipophilicity on the bioavailability of drugs after percutaneous administration by dissolving microneedles. (2012)
  • Author(s): Ito Y, Yoshimura M, Tanaka T and Takada K.
  • Journal Title: J. Pharm. Sci. Volume: 15 Pages: 145-56
  • Peer Reviewed
• [Journal Article] Effect of lipophilicity on the bioavailability of drugs after percutaneous administration by dissolving microneedles. (2012)
  • Author(s): Yukako Ito
  • Journal Title: J. Pharm. Sci. Volume: 101 Pages: 1145-1156
  • Peer Reviewed
• [Journal Article] Two- and three-layered dissolving microneedles for transcutaneous delivery of model vaccine antigen in rats. (2012)
  • Author(s): Asuka Ikejiri
  • Journal Title: J. Biomaterials Nanobiotechnol. Volume: 3 Pages: 325-334
  • Peer Reviewed
• [Journal Article] High percutanous bioavailability of granisetron by dissolving microneedles. (2012)
  • Author(s): Asuka Ikejiri
  • Journal Title: J. Drug Del. Sci. Tech. Volume: 22 Pages: 500-504
  • Peer Reviewed
• [Journal Article] Transdermal Insulin application system with dissolving microneedle. (2012)
  • Author(s): Yukako Ito
  • Journal Title: Diabetes Technol. Ther. Volume: 14 Pages: 891-899
  • Peer Reviewed
• [Journal Article] Incidence of low bioavailability of leuprolide acetate after percutaneous administration to rats by dissolving microneedles. (2011)
  • Author(s): Yukako Ito, Hiroshi Murano Noriyuki Hamasaki, Keizo Fukushima and Kanji Takada
  • Journal Title: Int. J. Pharm. Volume: 407 Pages: 126-131
  • Peer Reviewed
• [Journal Article] Insulin micropilescomprising biodegradable polymers for production of a long-term hypoglycemic effect. (2011)
  • Author(s): Keizo Fukushima, Yukako Ito, Masahiro Ishihata, Nobuyuki Sugioka and Kanji Takada
  • Journal Title: J.Drug Target. Volume: 19 Pages: 212-218
  • Peer Reviewed
• [Journal Article] Two-layered dissolving microneedles for percutaneous delivery ofsumatriptan. (2011)
  • Author(s): Yukako Ito, Sumie Kashihara, Keizo Fukushima and Kanji Takada
  • Journal Title: Drug. Dev. Ind. Pharm. Volume: 37 Pages: 1387-1393
  • Peer Reviewed
• [Journal Article] Incidence of low bioavailability of leuprolide acetate after percutaneous administration to rats by dissolving microneedles (2011)
  • Author(s): Yukako Ito
  • Journal Title: Int.J.Pharm Volume: 407 Pages: 126-131
  • Peer Reviewed
• [Journal Article] Insulin micropiles comprising biodegradable polymers for production of a long-term hypoglycemic effect (2011)
  • Author(s): Keizo Fukushima
  • Journal Title: J.Drug Target Volume: 37 Pages: 335-341
  • Peer Reviewed
• [Journal Article] Pharmacokinetics of clomipramine, an antidepressant, in poloxamer 407-induced hyperlipidaemic model rats (2011)
  • Author(s): Shinji Kobuchi
  • Journal Title: J.Pharm.Pharmacol. Volume: 63 Pages: 300-305
  • Peer Reviewed
• [Journal Article] Pharmacokinetics of clomipramine, an antidepressant, in poloxamer 407-induced hyperlipidaemic model rats (2011)
  • Author(s): Kenjiro Koga
  • Journal Title: J.Pharm.Pharmacol Volume: 63 Pages: 515-523
  • Peer Reviewed
• [Journal Article] Two-layered dissolving microneedles for percutaneous delivery of sumatriptan (2011)
  • Author(s): Yukako Ito
  • Journal Title: Drug.Dev.Ind.Pharm Volume: 37 Pages: 1387-1393
  • Peer Reviewed
• [Journal Article] Effect of oxidative stress on the pharmacokinetics of clomipramine rats treated with ferric-nitrilotriacetate (2011)
  • Author(s): Shinji Kobuchi
  • Journal Title: Drug Metab.Lett Volume: 5 Pages: 243-252
  • Peer Reviewed
• [Journal Article] Decrease in brain distribution of fluvoxamine in experimental hyperlipidemic rats (2011)
  • Author(s): Keizo Fukushima
  • Journal Title: J.Pharm.Pharmaceut.Sci Volume: 14 Pages: 414-424
  • Peer Reviewed
• [Journal Article] Effect of obesity induced by high-fat diet on the pharmacokinetics of cyclosporine A in rats (2011)
  • Author(s): Keizo Fukushima
  • Journal Title: Biomed.Preventive Nutr Volume: 1 Pages: 195-201
  • Peer Reviewed
• [Journal Article] Incidence of low bioavailability of leuprolide acetate after percutaneous administration to rats by dissolving microneedles. (2011)
  • Author(s): Y.Ito
  • Journal Title: Int.J.Pharmaceut Volume: 407 Pages: 126-131
  • Peer Reviewed
• [Journal Article] Insulin micropiles comprising biodegradable polymers for production of a long-term hypoglycemic effect. (2011)
  • Author(s): K.Fukushima
  • Journal Title: J.Drug Target Volume: 19 Pages: 212-218
  • Peer Reviewed
• [Journal Article] Utility of nano-sized, water-in-oil emulsion as a sustained release formulation of glycyrrhizin. (2011)
  • Author(s): K.Koga
  • Journal Title: Biol.Pharm.Bull Volume: 34 Pages: 300-305
  • Peer Reviewed
• [Journal Article] Two-layered dissolving microneedles for percutaneous delivery of peptide/protein drugs in rats. (2011)
  • Author(s): K.Fukushima
  • Journal Title: Pharm.Res. Volume: 28 Pages: 7-21
  • Peer Reviewed
• [Journal Article] Self-dissoving micropile array tips for percutaneous administration of insulin. (2010)
  • Author(s): Yukako Ito, Takenao Yamazaki, Nobuyuki Sugioka and Kanji Takada
  • Journal Title: J. Mater. Sci: Mater. Med. Volume: 21 Pages: 835-841
  • Peer Reviewed
• [Journal Article] Self-dissoving micropile array tips as percutaneous delivery system of protein drug. (2010)
  • Author(s): Yukako Ito, Ryo Hasegawa, KeizoFukushima, Nobuyuki Sugioka and Kanji Takada
  • Journal Title: Biol. Pharm. Bull. Volume: 33 Pages: 683-690
  • Peer Reviewed
• [Journal Article] Molecular weight dependence on bioavailability of FITC-dextran after afministration of self-dissolving micropile to rat skin. Drug. Dev. (2010)
  • Author(s): Yukako Ito, Ayaka Ise, Nobuyuki Sugioka and Kanji Takada
  • Journal Title: Ind. Pharm. Volume: 36 Pages: 845-851
  • Peer Reviewed
• [Journal Article] Evaluation of insulin self-dissolving micropiles by PK/PD analysis. (2010)
  • Author(s): K.Fukushima
  • Journal Title: J.Drug Del.Sci.Tech. Volume: 20 Pages: 361-336
  • Peer Reviewed
• [Journal Article] Molecular weight dependence on bioavailability of FITC-dextran after administration of self-dissolving micropile to rat skin. (2010)
  • Author(s): Y.Ito
  • Journal Title: Drug Dev.Ind.Pharm Volume: 36 Pages: 845-851
  • Peer Reviewed
• [Journal Article] Self-dissolving microarray chip as percutaneous delivery system of protein drug. (2010)
  • Author(s): Y.Ito
  • Journal Title: Biol.Pharm.Bull. Volume: 33 Pages: 683-690
  • Peer Reviewed
• [Journal Article] Permeation enhancement of ascorbic acid by self-dissolving micropile array tip through rat skin. (2010)
  • Author(s): Y.Ito
  • Journal Title: Chem.Pharm.Bull. Volume: 58 Pages: 458-463
  • NAID: 130000255937
  • Peer Reviewed
• [Journal Article] Self-dissolving micropile array tips for percutaneous administration of insulin. (2010)
  • Author(s): Y.Ito
  • Journal Title: J.Mater.Sci-Mater Med Volume: 21 Pages: 835-841
  • Peer Reviewed
• [Journal Article] Three-layered microcapsules as a long-term sustained release injection preparation. (2010)
  • Author(s): Y.Ito
  • Journal Title: Int.J.Pharmaceut Volume: 384 Pages: 53-59
  • Peer Reviewed
• [Journal Article] Nano-sized water-in-oil-in-water emulsion enhances intestinal absorption of calcein, a high solubility and low permeability compound. (2010)
  • Author(s): K.Koga
  • Journal Title: Eur.J.Pharm.Biopharm Volume: 74 Pages: 223-232
  • Peer Reviewed
• [Presentation] Usefulness of three-layered dissolving microneedles for skin vaccination. (2012)
  • Author(s): Kanji Takada, Seoshiro Naito, Yukako Ito, Mangatt Sudhakaran S., Asuka Ikejiri
  • Organizer: 第16回日本ワクチン学会学術集会
  • Place of Presentation: 横浜
• [Presentation] 塩基性繊維芽細胞増殖因子(bFGF)含有マイクロニードル・アレイ・チップに関する基礎的研究. (2012)
  • Author(s): 高田寛治、小野一郎、伊藤由佳子、上田祐理子、松地大輝、家田和奈、櫻井紀代美
  • Organizer: 第42回日本創傷治癒学会
  • Place of Presentation: 札幌
• [Presentation] 塩基性繊維芽細胞増殖因子（bFGF）含有マイクロニードル・アレイ・チップに関する基礎的研究 (2012)
  • Author(s): 高田寛治
  • Organizer: 第42回日本創傷治癒学会
  • Place of Presentation: 札幌
• [Presentation] 塩基性繊維芽細胞増殖因子（bFGF）マイクロニードル・アレイ・チップの瘢痕抑制効果に関する研究. (2012)
  • Author(s): 高田寛治
  • Organizer: 第42回日本創傷治癒学会
  • Place of Presentation: 札幌
• [Presentation] 民族差、地域差とグローバル開発 (2011)
  • Author(s): 高田寛治
  • Organizer: 第32回日本臨床薬理学会年会
  • Place of Presentation: アクトシティ浜松(静岡県)(招待講演)
  • Year and Date: 2011-12-02
• [Presentation] Effect of oxidative stress on the pharmacokineitcs of clomipramine and distribution to the brain (2011)
  • Author(s): Shinji Kobuchi
  • Organizer: 第26回日本薬物動態学会年会
  • Place of Presentation: 広島国際会議場(広島県)
  • Year and Date: 2011-11-17
• [Presentation] マイクロニードル投与用デバイス. (2011)
  • Author(s): 濱崎紀行、仲東卓也、伊藤由佳子、高田寛治
  • Organizer: 第27回日本DDS学会
  • Place of Presentation: 東京大学本郷キャンパス
• [Presentation] Epidermal vaccination of hepatitis A using a dissolving microneedle array. (2011)
  • Author(s): Seishiro Naito, Yukako Ito, Tomoko Kiyohara, Michiyo Kataoka, Masaki Ochiai, Kanji Takada
  • Organizer: 5th Vaccine ISV Annual Global Congress
  • Place of Presentation: Seattle, U.S.A.
• [Presentation] Transdermal insulin application system with dissolvingmicroneedles. (2011)
  • Author(s): Kanji Takada, Yukako Ito
  • Organizer: 11th Annual DiabetesTechnology Meeting
  • Place of Presentation: San Francisco, U.S. A.
• [Presentation] マイクロニードル投与用デバイス (2011)
  • Author(s): 濱崎紀行
  • Organizer: 第27回日本DDS学会
  • Place of Presentation: 東京大学本郷キャンパス(東京都)
• [Presentation] Epidermal vaccination of hepatitis A using a dissolving microneedle array (2011)
  • Author(s): Seishiro Naito
  • Organizer: 5^<th> Vaccine ISV Annual Global Congress
  • Place of Presentation: Seattle Marriott Waterfront Hotel (Seattle, U.S.A)
• [Presentation] Transdermal insulin application system with dissolving microneedles (2011)
  • Author(s): Kanji Takada
  • Organizer: 11^<th> Annual Diabetes Technology Meeting
  • Place of Presentation: Regency San Francisco Airport (California, U.S.A)
• [Presentation] マイクロニードル・アレイの皮膚侵入性に関する種差 (2011)
  • Author(s): 元谷俊彦
  • Organizer: 第32回日本臨床薬理学会年会
  • Place of Presentation: アクトシティ浜松(静岡県)(招待講演)
• [Presentation] Factors affecting the bioavailability of drugs from dissolving microneedles in rats. (2010)
  • Author(s): Yukako Ito, Keizo Fukushima, Nobuyuki Sugioka and Kanji Takada
  • Organizer: First International Conference on microneedles
  • Place of Presentation: Atlanta, USA
• [Presentation] Dissolving microneedles for percutaneous delivery ofinsulin. European Association for the study of Diabetes (2010)
  • Author(s): Kanji Takada and Yukako Ito
  • Organizer: 46th Annual Meeting
  • Place of Presentation: Stockholm, Sweden
• [Presentation] 経皮ワクチン用2層、3層マイクロニードル・アレイの比較 (2010)
  • Author(s): 池尻亜砂佳
  • Organizer: 第14回日本ワクチン学会学術集会
  • Place of Presentation: 東京
• [Presentation] 免疫抑制剤シクロスポリンの血中動態に及ぼす脂肪乳剤の影響 (2010)
  • Author(s): 松田達馬
  • Organizer: 第31回日本臨床薬理学会年会
  • Place of Presentation: 京都
• [Presentation] 日米欧における臨床用量の差異と血中動態 (2010)
  • Author(s): 高田寛治
  • Organizer: 第31回日本臨床薬理学会年会
  • Place of Presentation: 京都
• [Presentation] 生体肝移植における免疫抑制剤タクロリムスの血中動態に影響を与える諸因子 (2010)
  • Author(s): 木村嘉彦
  • Organizer: 第20回日本医療薬学会年会
  • Place of Presentation: 千葉
• [Presentation] Time-dependent interaction of ritonavir with p-glycoprotein and cytochrome P450 substrates in chronic use. (2010)
  • Author(s): 福島恵造
  • Organizer: 第25回日本薬物動態学会年会
  • Place of Presentation: 東京
• [Presentation] Effect of oxidative stress on the pharmacokineitcs of atazanavir. (2010)
  • Author(s): 青山洋昭
  • Organizer: 第25回日本薬物動態学会年会
  • Place of Presentation: 東京
• [Presentation] Dissolving microneedles for percutaneous delivery of insulin. (2010)
  • Author(s): 高田寛治
  • Organizer: European Association for the study of Diabetes, 46th Annual Meeting
  • Place of Presentation: Stockholm, Sweden
• [Presentation] 2層マイクロニードル (2010)
  • Author(s): 高田寛治
  • Organizer: バイオアカデミックフォーラム
  • Place of Presentation: 東京
• [Presentation] マイクロニードルアレイチップからの薬物のバイオアベイラビリティに及ぼす脂溶性要因の影響 (2010)
  • Author(s): 吉村昌寛
  • Organizer: 日本薬剤学会第25年会
  • Place of Presentation: 徳島
• [Book] 第12章3節.経皮投与(マイクロニードル)技術の動向.ワクチンの市場動向と開発・製造実務集 (2012)
  • Author(s): 高田寛治
  • Publisher: 技術情報協会
• [Book] ワクチン (2012)
  • Author(s): 高田寛治
  • Total Pages: 900
  • Publisher: 技術情報協会
• [Remarks] 京都薬科大学薬物動態学分野ホームページ
  • URL: http://www.kyoto-phu.ac.jp/labo/doutai/doutai-j.html
• [Remarks] 2011年11月9日の日本テレビニュースにてマイクロニードルワクチンが全国ネットで報道された。目下、高田寛治が理事長を務めるNPO日本健康支援機構ANのホームページで再生中である。
  • URL: http://www.annpo.jp/
• [Patent(Industrial Property Rights)] ワクチン抗原表皮デリバリー用溶解性3層マイクロニードル・アレイ・パッチ (2010)
  • Inventor(s): 高田寛治
  • Industrial Property Rights Holder: (株)バイオセレンタック
  • Filing Date: 2010-10-27