Regulation of gene expression by chromosome condensation protein, condensin

• Project/Area Number: 22310116
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genome biology
• Research Institution: University of Tsukuba
• Principal Investigator: KIMURA Keiji 筑波大学, 生命環境系, 准教授 (50332268)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000); Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 遺伝子発現制御 / クロマチン / コンデンシン / 核小体 / 細胞周期 / リン酸化 / 染色体凝縮 / 細胞体凝縮 / 転写制御 / 染色体

Research Abstract

ChIP assay indicated that both isoform of condensin (I and II) presented on the rDNA region in the interphase nucleolus. Knockdown of condensin (I or II) led to enhanced acetylation of histones in rRNA region, suggesting that condensin condenses interphase chromatin structures and suppresses gene expressions. We also found that dephosphorylation of condensin (I and II) by PP1 and PP2A at the exit of mitosis was important for dissociation of condensin from mitotic chromosomes.

Research Products

• [Journal Article] Enhancer of rudimentary homolog (ERH) plays an essential role in the progression of mitosis by promoting mitotic chromosome alignment (2012)
  • Author(s): Fujimura A, Kishimoto H, Yanagisawa J, Kimura K
  • Journal Title: Biochem Biophys Res Commun. Volume: 423 Pages: 588-592
  • Peer Reviewed
• [Journal Article] Enhancer of rudimentary homolog (ERH) plays an essential role in the progression of mitosis by promoting mitotic chromosome alignment. (2012)
  • Author(s): Fujimura A, Kishimoto H, Yanagisawa J, Kimura K.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 423 Issue: 3 Pages: 588-592
  • DOI: 10.1016/j.bbrc.2012.06.018
  • Peer Reviewed
• [Journal Article] Estrogen regulates tumor growth through a nonclassical pathway that includes the transcription factors ERβ and KLF5 (2011)
  • Author(s): Nakajima Y, Akaogi K, et al
  • Journal Title: Science Signaling Volume: Vol.4 Issue: 168
  • DOI: 10.1126/scisignal.2001551
  • Peer Reviewed
• [Journal Article] Novel nucleolar pathway connecting intracellular energy status with p53 activation (2011)
  • Author(s): Kumazawa T, Nishimura K, Kuroda T, Ono W, Yamaguchi C, Katagiri N, Tsuchiya M, Masumoto H, Nakajima Y, Murayama A, Kimura K, Yanagisawa J
  • Journal Title: J. Biol. Chem. Volume: 288 Pages: 20861-20869
  • Peer Reviewed
• [Journal Article] Critical role of the nucleolus in activation of the p53-dependent postmitotic checkpoint (2011)
  • Author(s): Tsuchiya M, Katagiri N, Kuroda T, Kishimoto H, Nishimura K, Kumazawa T, Iwasaki N, Kimura K, Yanagisawa J
  • Journal Title: Biochem Biophys Res Commun. Volume: 407 Pages: 378-382
  • Peer Reviewed
• [Journal Article] RNA content in the nucleolus alters p53 acetylation via MYBBP1A (2011)
  • Author(s): Kuroda T, Murayama A, Katagiri N, Ohta Y, Fujita E, Masumoto H, Ema M, Takahasi S, Kimura K, Yanagisawa J
  • Journal Title: EMBO J. Volume: 30 Pages: 1054-1066
  • Peer Reviewed
• [Journal Article] Novel nucleolar pathway connecting intracellular energy status with p53 activation. (2011)
  • Author(s): Kumazawa T, Nishimura K, Kuroda T, Ono W, Yamaguchi C, Katagiri N, Tsuchiya M, Masumoto H, Nakajima Y, Murayama A, Kimura K, & Yanagisawa J.
  • Journal Title: J. Biol. Chem. Volume: 288 Issue: 23 Pages: 20861-20869
  • DOI: 10.1074/jbc.m110.209916
  • Peer Reviewed
• [Journal Article] Critical role of the nucleolus in activation of the p53-dependent postmitotic checkpoint. (2011)
  • Author(s): Tsuchiya M, Katagiri N, Kuroda T, Kishimoto H, Nishimura K, Kumazawa T, Iwasaki N, Kimura K, & Yanagisawa J.
  • Journal Title: Biochem Biophys Res. Commun. Volume: 407 Issue: 2 Pages: 378-382
  • DOI: 10.1016/j.bbrc.2011.03.029
  • Peer Reviewed
• [Journal Article] Novel nucleolar pathway connecting intracellular energy status with p53 activation (2011)
  • Author(s): Kumazawa T, Nishimura K, Kuroda, T, Ono W, Yamaguchi C, Katagiri N, Tsuchiya M, Masumoto H, Nakajima Y, Murayama A, Kimura K, Yanagisawa J
  • Journal Title: J.Biol.Chem Volume: 286 Pages: 20861-20869
  • Peer Reviewed
• [Journal Article] Critical role of the nucleolus in activation of the p53-dependent postmitotic checkpoint (2011)
  • Author(s): Tsuchiya M, Katagiri N, Kuroda T, Kishimoto H, Nishimura K, Kumazawa T, Iwasaki N, Kimura K, Yanagisawa J
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 407 Pages: 378-382
  • Peer Reviewed
• [Journal Article] Estrogen regulates tumor growth through a nonclassical pathway that includes the transcription factors ERβ and KLF5 (2011)
  • Author(s): Nakajima Y, Osakabe A, Suzuki T, Kishimoto H, Kuroda T, Fujimura T, Homma Y, Murayama A, Kimura K, Inoue S, Yanagisawa J
  • Journal Title: Science Signaling Volume: 4
  • Peer Reviewed
• [Journal Article] Estrogen inhibits transforming growth factor beta signaling by promoting Smad2/3 degradation (2010)
  • Author(s): Ito I, Hanyu A, Wayama M, Goto N, Katsuno Y, Kawasaki S, Nakajima Y, Kajiro M, Komatsu Y, Fujimura A, Hirota R, Murayama A, Kimura K, Imamura T, Yanagisawa J
  • Journal Title: J.Biol.Chem Volume: 285 Pages: 14747-14755
  • Peer Reviewed
• [Presentation] 核小体因子によるM期染色体動態制御 (2012)
  • Author(s): 木村圭志
  • Organizer: 第30回染色体ワークショップ第11回核ダイナミクス研究会
  • Place of Presentation: 淡路
  • Year and Date: 2012-12-19
• [Presentation] Nucleolar factors regulate faithful mitotic chromosome segregation (2012)
  • Author(s): Kimura K, Fujimra A, Kishimoto H, Yanagisawa J
  • Organizer: 第8回3Rシンポジウム
  • Place of Presentation: 淡路
  • Year and Date: 2012-11-25
• [Presentation] ホスファターゼによる染色体分配制御 (2012)
  • Author(s): 木村圭志
  • Organizer: 「細胞増殖制御」終了シンポジウム
  • Place of Presentation: 東京工業大学
  • Year and Date: 2012-08-30
• [Presentation] 核小体因子NOL11によるAurora Bを介したM期染色体動態制御 (2012)
  • Author(s): 木村圭志、藤村亜紀子、柳澤純
  • Organizer: 第29回染色体ワークショップ
  • Place of Presentation: 仙台
  • Year and Date: 2012-01-26
• [Presentation] 核小体RNAとタンパク質による協調的なM期染色体動態制御 (2012)
  • Author(s): 木村 圭志
  • Organizer: 「ゲノム普遍席制御」第3回領域会議
  • Place of Presentation: 淡路・淡路夢舞台国際会議所
• [Presentation] 核小体RNAとタンパク質によるM期染色体動態制御 (2012)
  • Author(s): 木村 圭志
  • Organizer: 遺伝情報場 第５回領域班会議
  • Place of Presentation: 北海道・ルスツリゾート
• [Presentation] Nucleolar factors regulate faithful mitotic chromosome segregation (2012)
  • Author(s): Kimura K, Fujimura A, Kishimoto H, Yanagisawa J.
  • Organizer: 第8回3Rシンポジウム
  • Place of Presentation: 淡路・淡路夢舞台国際会議所
• [Presentation] ホスファターゼの新規機能を介した染色体動態制御 (2011)
  • Author(s): 木村圭志
  • Organizer: 細胞増殖制御班会議
  • Place of Presentation: 鳥取大山ロイヤルホテル
  • Year and Date: 2011-09-07
• [Presentation] 核小体を介したM期染色体ダイナミクスの解析 (2011)
  • Author(s): 木村圭志
  • Organizer: 遺伝情報場班会議
  • Place of Presentation: 北海道大学ルスツリゾート
  • Year and Date: 2011-06-11
• [Presentation] A dual function of PP2A to regulate the localization of chromosomal proteins (2010)
  • Author(s): 木村圭志
  • Organizer: 第33回日本分子生物学会年会シンポジウム
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-18
• [Presentation] A dual function on PP2A to regulate the localization of chromosomal proteins (2010)
  • Author(s): Kimura, K.
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(招待講演)
  • Year and Date: 2010-12-08
• [Presentation] Regulation of condensin by dual functions of PP2A (2010)
  • Author(s): 木村圭志
  • Organizer: MEXT Priority Research Project"Cell Proliferation Control"International Symposium
  • Place of Presentation: Tokyo
  • Year and Date: 2010-11-08
• [Presentation] Regulation of condensin by the dual functions of PP2A (2010)
  • Author(s): Kimura, K.
  • Organizer: MEXT Priority Research Project "Cell Proliferation Control" International Workshop MITOSIS : Cell Proliferation Control
  • Place of Presentation: 癌研究所(東京)(招待講演)
  • Year and Date: 2010-11-08
• [Presentation] PP2Aの新規機能による染色体タンパク質の制御機構 (2010)
  • Author(s): 木村圭志
  • Organizer: 癌研究所セミナー
  • Place of Presentation: 癌研究所
  • Year and Date: 2010-08-06
• [Presentation] PP2Aの新規機能による染色体タンパク質の制御機構 (2010)
  • Author(s): 木村圭志
  • Organizer: 癌研究所セミナー
  • Place of Presentation: 癌研究所(東京)
  • Year and Date: 2010-08-06
• [Presentation] Regulation of condensing by the dual functions of PP2A (2010)
  • Author(s): Takemoto A, Maeshima K, Ikehara T, Hanaoka F, Yanagisawa J, Kimura K
  • Organizer: Cold Spring Harbor Symposium Cold Spring Harbor
  • Place of Presentation: NY, USA
  • Year and Date: 2010-06-04
• [Presentation] The localization of condensin II is regulated by a non-catalytic action of PP2A (2010)
  • Author(s): Takemoto, A., Maeshima, K, Ikehara, T., Hanaoka, R, Yanagisawa, J., Kimura, K.
  • Organizer: Cold Spring Harbor Symposium
  • Place of Presentation: Cold Spring Harbor, NY, USA
  • Year and Date: 2010-06-04
• [Presentation] PP2Aによるコンデンシンを介した染色体構造制御 (2010)
  • Author(s): 竹本愛、前島一博、柳澤純、木村圭志
  • Organizer: 第9回核ダイナミクス研究会
  • Place of Presentation: 伊豆市
  • Year and Date: 2010-05-28
• [Presentation] ホスファターゼによる染色体分配制御
  • Author(s): 木村 圭志
  • Organizer: 「細胞増殖制御」終了シンポジウム
  • Place of Presentation: 神奈川・東京工業大学
• [Presentation] 核小体因子によるM期染色体動態制御
  • Author(s): 木村 圭志
  • Organizer: 第30回染色体ワークショップ 第11回核ダイナミクス研究会
  • Place of Presentation: 淡路・淡路夢舞台国際会議所
• [Presentation] 核小体因子NOL11によるAurora Bを介したM期染色体動態制御
  • Author(s): 木村圭志、藤村亜希子、柳澤純
  • Organizer: 第29回染色体ワークショップ
  • Place of Presentation: 仙台,秋保温泉 ホテルニュー水戸屋
• [Presentation] Nucleolar factors regulate faithful mitotic chromosome segregation
  • Author(s): Kimura K, Fujimura A, Kishimoto H, & Yanagisaw J.
  • Organizer: 第8回国際3Rシンポジウム
  • Place of Presentation: 淡路,兵庫県立淡路夢舞台国際会議場
• [Presentation] 核小体因子によるM期染色体の動態制御
  • Author(s): 木村圭志
  • Organizer: 第30回染色体ワークショップ・第11回核ダイナミクス研究会合同
  • Place of Presentation: 淡路,兵庫県立淡路夢舞台国際会議場
• [Book] M期を制御するホスファターゼとその新規機能(597-602) (2011)
  • Author(s): 竹本愛、木村圭志
  • Publisher: 秀潤社
• [Book] 細胞工学「M期を制御するホスファターゼとその新規機能」 (2011)
  • Author(s): 竹本 愛、木村 圭志
  • Total Pages: 6
  • Publisher: 秀潤社
• [Book] 細胞工学Vol.30,No.6 (2011)
  • Author(s): 竹本愛、木村圭志
  • Total Pages: 6
  • Publisher: 秀潤社