Identification and biochemical analyses of novel factors that are involved in homologous recombination reaction
| Project/Area Number |
22310119
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | Waseda University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2011: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2010: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | ゲノム維持修復 / ゲノム多様性 / ゲノム進化・再編 / 遺伝情報複製・再編 / ゲノム機能 / 活性発現の分子機構 / 染色体構築・機能・分配 / 生体高分子構造・機能 / 構造活性相関 |
|---|
| Research Abstract |
Homologous recombination functions in DNA double strand break repair and meiotic recombination. Several proteins that are involved in the homologous recombination have been identified. However, missing recombination factors still exist in the homologous-recombination pathway. In this study, in humans, GEMIN2, EVL, and PSF, have been identified as novel homologous recombination proteins. Biochemical analyses with purified proteins revealed their functions in homologous recombination in vitro. In addition, we also found that FANCD2, an essential recombination protein, is robustly monoubiquitinated in the presence of DNA and FANCI.
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Report
(4 results)
Research Products
(151 results)
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[Journal Article] The ATR-ATRIP kinase complex is responsible for triggering activation of the FA pathway2012
Author(s)
Shigechi T., Tomida J., Sato K., Kobayashi M., Eykelenboom J. K., Pessina F., Zhang Y., Uchida E., Ishiai M., Lowndes N. F., Yamamoto K., Kurumizaka H., Maehara Y., Takata M
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Journal Title
Cancer Res.
Volume: 72
Pages: 1149-1156
Related Report
Peer Reviewed
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[Journal Article] RAD51 plays a crucial role in halting cell death program induced by ionizing radiation in bovine oocytes2012
Author(s)
Kujjo L.L., Ronningen R., Ross P., Pereira R.J.G., Rodriguez R., Beyhan Z., Goissis M.D., Baumann T., Kagawa W., Camsari C., Smith G.W., Kurumizaka H., Yokoyama S., Cibelli J.B., Perez G.I
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Journal Title
Biol. Reprod.
Volume: 86
Pages: 76-76
Related Report
Peer Reviewed
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[Journal Article] ATR-ATRIP Kinase Complex Triggers Activation of the Fanconi Anemia DNA Repair Pathway2012
Author(s)
Tomoko Shigechi, Junya Tomida, Koichi Sato, Masahiko Kobayashi, John K.Eykelenboom, Fabio Pessina, Yanbin Zhang, Emi Uchida, Masamichi Ishiai, Noel F.Lowndes, Kenichi Yamamoto, Hitoshi Kurumizaka, Yoshihiko Maehara, Minoru Takata
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Journal Title
Cancer Research
Volume: 72
Issue: 5
Pages: 1149-1156
DOI
Related Report
Peer Reviewed
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[Journal Article] Purification of the Human SMN-GEMIN2 Complex and Assessment of Its Stimulation of RAD51-Mediated DNA Recombination Reactions2011
Author(s)
Motoki Takaku, Takashi Tsujita, Naoki Horikoshi, Yoshimasa Takizawa, Yong, Qing, Kouji Hirota, Masae Ikura, Tsuyoshi Ikura, Shunichi Takeda, Hitoshi Kurumizaka
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Journal Title
Biochemistry
Volume: 50
Issue: 32
Pages: 6797-6805
DOI
Related Report
Peer Reviewed
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[Journal Article] GEMIN2 promotes accumulation of RAD51 at double-strand breaks in homologous recombination.2010
Author(s)
Takizawa Y., Qing Y., Takaku M., Ishida T., Morozumi Y., Tsujita T., Kogame T., Hirota K., Takahashi M., Shibata T., Kurumizaka H., Takeda S
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Journal Title
Nucleic Acids Res.
Volume: 38
Pages: 5059-5074
Related Report
Peer Reviewed
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[Presentation] Histone chaperon activity of Fanconi anemia proteins, FANCD2 and FANCI, is required for DNA crosslink repair2012
Author(s)
Ishiai M., Sato K., Toda K., Furukoshi S., Osakabe A., Tachiwana H., Takizawa Y., Kagawa W., Kitao H., Dohmae N., Obuse C., Kimura H., Takata M., and Kurumizaka H
Organizer
The 8th 3R Symposium
Place of Presentation
兵庫
Related Report
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[Presentation] Human telomeric repeat sequences cause destabilization of nucleosome positioning in yeast cells2012
Author(s)
Satome-Nakamura A., Yasuda T., Ogi T., Nakazawa Y., Saito K., Kagawa W., Suzuki T., Dohmae N., Hino T., Kato T., Genet M., Hazawa M., Tomita M., Hanaoka F., Sugasawa K., Jeggo P., Okayasu R., and Kurumizaka H
Organizer
The 8th 3R Symposium
Place of Presentation
兵庫
Related Report
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[Presentation] Biochemical studies of homologous pairing on chromatinized DNA2012
Author(s)
Takaku M., Machida S., Kobayashi W., Hayashida R., Suzuki H., Ikura M., Osakabe A., Tachiwana H., Tashiro S., Ikura T., and Kurumizaka H
Organizer
The 8th 3R Symposium
Place of Presentation
兵庫
Related Report
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[Presentation] Acetylation of human Rad52 protein CBP/p3002012
Author(s)
Yasuda T., Saito K., Kagawa W., Ogi T., Suzuki T., Dohmae N., Hino T., Nakazawa Y., Satome-Nakamura A., Kato T., Genet M., Hazawa M., Tomita M., Hanaoka F., Sugasawa K., Jeggo P., Okayasu R., Tajima K., and Kurumizaka H
Organizer
The 8th 3R Symposium
Place of Presentation
兵庫
Related Report
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[Presentation] ATR-ATRIP kinase complex is responsible for triggering activation of the FA pathway2011
Author(s)
Shigechi T., Tomida J., Sato K., Kobayashi M., Eykelenboom J., Fabio P., Yanbin Z., Uchida E., Ishiai M., Lowndes N., Yamamoto K., Kurumizaka H., Maehara Y., Takata M
Organizer
第34回日本分子生物学会年会
Place of Presentation
神奈川
Related Report
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[Presentation] Histone chaperone activity of the FANCI-FANCD2 complex and its importance in repair of inter-strand DNA crosslinks by the Fanconi anemia pathway2011
Author(s)
Sato K., Ishiai M., Toda K., Furukoshi S., Osakabe A., Tachiwana H., Takizawa Y., Kagawa W., Kitao H., Dohmae N., Obuse C., Kimura H., Takata M., Kurumizaka H
Organizer
第34回日本分子生物学会年会
Place of Presentation
神奈川
Related Report
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