Deciphering the regulatory logic for cell type-specific transcription based on intranuclear dynamics of transcription factors and cis-regulatory DNAs

• Project/Area Number: 22310120
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genome biology
• Research Institution: Konan University
• Principal Investigator: KUSAKABE TAKEHIRO 甲南大学, 理工学部, 教授 (40280862)
• Co-Investigator(Kenkyū-buntansha): NAKAI Kenta 東京大学, 医科学研究所, 教授 (60217643) ; SUZUKI Yutaka 東京大学, 大学院新領域創成科学研究科, 教授 (40323646) ; SHOGUCHI Eiichi 沖縄科学技術大学院大学, マリンゲノミックスユニット, ユニットリーダー (90378563)
• Project Period (FY): 2010-04-01 – 2013-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000); Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2011: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000); Fiscal Year 2010: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 遺伝子 / ゲノム / 転写調節 / 発生・分化 / ホヤ / メダカ

Research Abstract

To obtain a genome wide landscape of transcriptional activity in the simple chordate model Ciona intestinalis, we have conducted a large scale sequencing of 5' ends of their mRNAs by massively parallel sequencing using the Illumina Genome Analyzer. The obtained 5'-end sequences were then used to map transcription start sites (TSSs) on the genome. The data were also used to map 5'-ends of trans-spliced transcripts. These data have been used to determine the TSS of a trans-spliced gene and for genome-wide profiling of C. intestinalis promoters. Based on these data, we further analyzed cell type-specific gene regulatory mechanisms in vivo by examining function of transcription factors, cis-regulatory DNAs, and chromatin level interactions. Using medaka as a vertebrate model, we have revealed that physical interactions between upstream cis-regulatory regions of two adjacent genes are a widely conserved mechanism for spatially regulated transcription during embryogenesis.

Research Products

• [Journal Article] Characterization of the compact bicistronic microRNA precursor, miR-1/miR-133, expressed specifically in Ciona muscle tissues. (2013)
  • Author(s): Rie Kusakabe, Saori Tani, Koki Nishitsuji, Miyuki Shindo, Kohji Okamura, Yuki Miyamoto, Kenta Nakai, Yutaka Suzuki, Takehiro G Kusakabe, Kunio Inoue
  • Journal Title: Gene Expression Patterns Volume: 13 Issue: 1-2 Pages: 43-50
  • DOI: 10.1016/j.gep.2012.11.001
  • Peer Reviewed
• [Journal Article] Cis-acting transcriptional repression establishes a sharp boundary in chordate embrvos (2012)
  • Author(s): Imai KS, DaidoY, Kusakabe TG, Satou Y
  • Journal Title: Science Volume: 337 Issue: 6097 Pages: 964-967
  • DOI: 10.1126/science.1222488
  • Peer Reviewed
• [Journal Article] A conserved non-reproductive GnRH system in chordates (2012)
  • Author(s): Takehiro G. Kusakabe, Tsubasa Sakai, Masato Aoyama, Yuka Kitajima, Yuki Miyamoto, Toru Takigawa, Yutaka Daido, Kentaro Fujiwara, Yasuko Terashima, Yoko Sugiuchi, Giorgio Matassi, Hitoshi Yagisawa, Min Kyun Park, Honoo Satake, and Motoyuki Tsuda
  • Journal Title: PLoS One Volume: 7 (7) Issue: 7 Pages: e41955-e41955
  • DOI: 10.1371/journal.pone.0041955
  • Peer Reviewed
• [Journal Article] Monoaminergic modulation of photoreception in ascidian : Evidence for a proto-hypothalamo-retinal territory (2012)
  • Author(s): Florian Razy-Krajka, Euan R. Brown, Takeo Horie, Jacques Callebert, Yasunori Sasakura, Jean-Stéphane Joly, Takehiro G. Kusakabe, and Philippe Vernier
  • Journal Title: BMC Biology Volume: 10 Issue: 1 Pages: 45-45
  • DOI: 10.1186/1741-7007-10-45
  • Peer Reviewed
• [Journal Article] Cell lineage and cis-regulation for a unique GABAergic/glycinergic neuron type in the larval nerve cord of the ascidian Ciona intestinalis (2012)
  • Author(s): K.Nishitsuii
  • Journal Title: Development, Growth and Differentiation Volume: 54 Issue: 2 Pages: 177-186
  • DOI: 10.1111/j.1440-169x.2011.01319.x
  • Peer Reviewed
• [Journal Article] Profiling ascidian promoters as the primordial type of vertebrate promoter (2011)
  • Author(s): Kohji Okamura, Riu Yamashita, Noriko Takimoto, Koki Nishitsuji, Yutaka Suzuki, Takehiro G. Kusakabe, and Kenta Nakai
  • Journal Title: BMC Genomics Volume: 12 (Suppl. 3) Issue: S3
  • DOI: 10.1186/1471-2164-12-s3-s7
  • Peer Reviewed
• [Journal Article] COQ6 mutations in human patients produce nephrotic syndrome with sensorineural deafness (2011)
  • Author(s): Saskia F. Heeringa他56名
  • Journal Title: J. Clin. Invest Volume: 121 (5) Issue: 5 Pages: 2013-2024
  • DOI: 10.1172/jci45693
  • Peer Reviewed
• [Journal Article] Cross-validated methods for promoter/transcription start site mapping in SL trans-spliced genes, established using the Ciona intestinalis troponin I gene (2011)
  • Author(s): Parul Khare, Sandra I. Mortimer, Cynthia L. Cleto, Kohji Okamura, Yutaka Suzuki, Takehiro Kusakabe, Kenta Nakai, Thomas H. Meedel, and Kenneth E. M. Hastings
  • Journal Title: Nucleic Acids Res. Volume: 39 (7) Issue: 7 Pages: 2638-2648
  • DOI: 10.1093/nar/gkq1151
  • Peer Reviewed
• [Journal Article] Ependymal cells of chordate larvae are stem-like cells from the adult nervous system (2011)
  • Author(s): Horie, T., Shinki, R., Ogura, Y., Kusakabe, T.G., Satoh, N., and Sasakura, Y.
  • Journal Title: Nature Volume: 469 Issue: 7331 Pages: 525-528
  • DOI: 10.1038/nature09631
  • Peer Reviewed
• [Journal Article] 動物の発生と進化の遺伝子発現調節ネットワーク-ゲノムからの挑戦 (2011)
  • Author(s): 將口栄一
  • Journal Title: 生物の科学遺伝65.28-34 Volume: 65 Pages: 28-34
• [Journal Article] Ependymal cells of chordate larvae are stem-like cells that form the adult nervous system (2011)
  • Author(s): Takeo Horie
  • Journal Title: Nature Volume: 469 Pages: 525-528
  • Peer Reviewed
• [Journal Article] The ANISEED database : digital representation, formalization, and elucidation of a chordate developmental program (2010)
  • Author(s): O. Tassy, D. Dauga, F. Daian, D. Sobral, F. Robin, P. Khoueiry, D. Salgado, V Fox, D. Caillol, R. Schiappa, B. Laporte, A. Rios, G. Luxardi, T. Kusakabe, J. S. Joly, S. Darras, L. Christiaen, M. Contensin, H. Auger, C. Lamy, C. Hudson, U. Rothbacher, M. J. Gilchrist, K. W. Makabe, K. Hotta, S. Fujiwara, N. Satoh, Y Satou and P Lemaire
  • Journal Title: Genome Res Volume: 20 Issue: 10 Pages: 1459-1468
  • DOI: 10.1101/gr.108175.110
  • Peer Reviewed
• [Journal Article] Genomic cis-regulatory networks in the early Ciona intestinalis embryo (2010)
  • Author(s): A. Kubo, N. Suzuki, X. Yuan, K. Nakai, N. Satoh, K. S. Imai and Y Satou
  • Journal Title: Development Volume: 137 Issue: 10 Pages: 1613-1623
  • DOI: 10.1242/dev.046789
  • Peer Reviewed
• [Journal Article] Positional variations among heterogeneous nucleosome maps give dynamical information on chromatin (2010)
  • Author(s): Yoshiaki Tanaka, Itsuki Yoshimura, and Kenta Nakai
  • Journal Title: Chromosoma. Volume: 119 (4) Issue: 4 Pages: 391-404
  • DOI: 10.1007/s00412-010-0264-y
  • Peer Reviewed
• [Journal Article] The ANISEED database : Digital representation, formalization, and elucidation of a chordate developmental program (2010)
  • Author(s): Olivier Tassy
  • Journal Title: Genome Research Volume: 20 Pages: 1459-1468
  • Peer Reviewed
• [Journal Article] Genomic cis-regulatory networks in the Ciona intestinalis embryo (2010)
  • Author(s): Atsushi Kubo
  • Journal Title: Develogmemt Volume: 137 Pages: 1613-1623
  • Peer Reviewed
• [Journal Article] Gradual transition from mosaic to global DNA methylation patterns during deuterostome evolution (2010)
  • Author(s): Kohji Okamura
  • Journal Title: BMC Bioinformatics Volume: 11(Suppl 7)
  • Peer Reviewed
• [Journal Article] Positional variations among heterogeneous nucleosome maps give dynamical information on chromatin (2010)
  • Author(s): Yoshiaki Tanaka
  • Journal Title: Chromosoma Volume: 119 Pages: 391-404
  • Peer Reviewed
• [Presentation] ホヤのメチローム解析から探る組織特異的メチル化の起源 (2013)
  • Author(s): 横山貴央
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-05
• [Presentation] カタユウレイボヤにおける転写開始点およびプロモーターの網羅的解析 (2013)
  • Author(s): 横森類
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸
  • Year and Date: 2013-12-03
• [Presentation] Development and function of the sensory and motor systems in the simple chordate Ciona intestinalis (2013)
  • Author(s): Také Kusakabe
  • Organizer: OIST Winter Course"Evolution of Complex Systems"
  • Place of Presentation: Onna, Okinawa, Japan
  • Year and Date: 2013-12-03
• [Presentation] 錐体オプシン遺伝子と共発現するマイクロRNAの役割 (2013)
  • Author(s): 大道裕
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-27
• [Presentation] カタユウレイボヤの運動を制御する神経回路の微細構造解析 (2013)
  • Author(s): 柏木洋貴
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-27
• [Presentation] カタユウレイボヤ幼生の遊泳運動制御回路の機能解析 (2013)
  • Author(s): 島井光太郎
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-27
• [Presentation] Functional and structural evidence for a central pattern generator controlling swimming locomotion of the Ciona intestinalis larva (2013)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: 7th International Tunicate Meeting
  • Place of Presentation: Naples, Italy
  • Year and Date: 2013-07-25
• [Presentation] Identification and transcriptional regulation of a gene encoding a novel BMP-antagonist Pinhead (2013)
  • Author(s): Kaoru S. Imai
  • Organizer: 7th International Tunicate Meeting
  • Place of Presentation: Naples, Italy
• [Presentation] Cell-lineage analysis of the larval photoreceptor cells in Ciona intestinalis (2013)
  • Author(s): Moeko Tanaka
  • Organizer: 7th International Tunicate Meeting
  • Place of Presentation: Naples, Italy
• [Presentation] Tracking cells expressing Hedgehog signaling molecules during postembryonic development of Ciona intestinalis (2013)
  • Author(s): Megumi Mukai
  • Organizer: 7th International Tunicate Meeting
  • Place of Presentation: Naples, Italy
• [Presentation] Molecular characterization of Piwi in the central nervous system of the Ciona larva (2013)
  • Author(s): Kotaro Shimai
  • Organizer: 7th International Tunicate Meeting
  • Place of Presentation: Naples, Italy
• [Presentation] Ultrastructural analysis of the neural circuit for swimming locomotion of the Ciona intestinalis larva (2013)
  • Author(s): Hiroki Kashiwagi
  • Organizer: 7th International Tunicate Meeting
  • Place of Presentation: Naples, Italy
• [Presentation] Genome-wide analysis of transcription start sites and promoters in Ciona intestinalis (2013)
  • Author(s): Rui Yokomori
  • Organizer: 7th International Tunicate Meeting
  • Place of Presentation: Naples, Italy
• [Presentation] Shared cis-regulatory modules regulate transcription of evolutionarily conserved and bidirectionally transcribed miRNA-opsin gene pairs in the medaka retina (2013)
  • Author(s): Yutaka Daido
  • Organizer: 17th International Congress of Developmental Biology
  • Place of Presentation: Cancún, Mexico
• [Presentation] Cis-acting transcriptional repression establishes a sharp boundary in chordate embryos (2013)
  • Author(s): Kaoru Imai
  • Organizer: 17th International Congress of Developmental Biology
  • Place of Presentation: Cancún, Mexico
• [Presentation] A novel somatic role of Piwi in the central nervous system of the ascidian Ciona intestinalis (2013)
  • Author(s): Kotaro Shimai
  • Organizer: 17th International Congress of Developmental Biology
  • Place of Presentation: Cancún, Mexico
• [Presentation] Cis-acting transcriptional repression establishes a sharp boundary in chordate embryos(Admp遺伝子はPinhead遺伝子によりタンパク質レベルおよび転写レベルで二重の抑制をうけ動物胚の腹側の領域を決定する) (2013)
  • Author(s): Kaoru Imai
  • Organizer: 第46回日本発生生物学会大会
  • Place of Presentation: 松江
• [Presentation] Cone subtype-specific microRNAs as putative regulators for photoreceptor subtypes : evidence from primary transcripts localization and target prediction in medaka (2013)
  • Author(s): Yutaka Daido
  • Organizer: 第19回小型魚類研究会
  • Place of Presentation: 仙台
• [Presentation] Cone subtype-specific microRNAs as putative regulators for photoreceptor subtypes: evidence from primary transcripts localization and target prediction in medaka (2013)
  • Author(s): Yutaka Daido, Takehiro G. Kusakabe
  • Organizer: 19th Japanese Medaka and Zebrafish Meeting
  • Place of Presentation: 仙台
• [Presentation] Shared cis-regulatory modules regulate transcription of evolutionarily conserved and bidirectionally transcribed miRNA-opsin gene pairs in the medaka retina (2013)
  • Author(s): Yutaka Daido, Takehiro G. Kusakabe
  • Organizer: 17th International Congress of Developmental Biology
  • Place of Presentation: Cancun, Mexico
• [Presentation] A novel somatic role of Piwi in the central nervous system of the ascidian Ciona intestinalis (2013)
  • Author(s): Kotaro Shimai, Takeo Horie, Koki Nishitsuji, Maki Shirae-Kurabayashi, Akira Nakamura, Rie Kusakabe, Kenta Nakai, Kunio Inoue, Takehiro G. Kusakabe
  • Organizer: 17th International Congress of Developmental Biology
  • Place of Presentation: Cancun, Mexico
• [Presentation] Development and function of the sensory and motor systems in the simple chordate Ciona intestinalis (2013)
  • Author(s): Take Kusakabe
  • Organizer: OIST Winter Course “Evolution of Complex Systems”
  • Place of Presentation: Onna, Okinawa, Japan
• [Presentation] Cis-acting transcriptional repression establishes a sharp boundary in chordate embryos (2013)
  • Author(s): Kaoru Imai, Yutaka Daido, Takehiro Kusakabe, Yutaka Satou
  • Organizer: 第46回日本発生生物学会大会
  • Place of Presentation: 松江
• [Presentation] otxモルファントの光応答から見出されたホヤ幼生の眼外光受容細胞 (2012)
  • Author(s): 石本慎一郎
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] カタユウレイボヤ幼生の中枢パターン発生器を構成するGABA/グリシン作動性神経細胞のSoxB1による発生制御 (2012)
  • Author(s): 森口大輔
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] 共通のシス調節モジュールによる進化的に保存されたmiRNA-オプシン遺伝子対の錐体サブタイプ特異的な転写制御 (2012)
  • Author(s): 大道裕
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-14
• [Presentation] カタユウレイボヤ中枢神経系におけるPiwiのはたらき (2012)
  • Author(s): 島井光太郎
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] ホヤ器官形成過程におけるhedgehogおよびGli発現細胞の挙動とヘッジホッグシグナルの役割 (2012)
  • Author(s): 向恵実
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] Deep sequencing analysis of transcription start sites (TSSs) in Ciona intestinalis (2012)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: 2nd International Workshop on Tunicate Information System
  • Place of Presentation: 京都
  • Year and Date: 2012-11-26
• [Presentation] Medaka as a model system to extend novel findings in ascidians to vertebrates (2012)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都
  • Year and Date: 2012-09-23
• [Presentation] Transcriptional co-regulation of the bidirectionally transcribed cone opsin-miRNA gene pairs in the medaka Oryzias latipes (2012)
  • Author(s): Yutaka Daido
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都
  • Year and Date: 2012-09-23
• [Presentation] カタユウレイボヤの器官形成過程におけるhedgehogおよびGli発現細胞の追跡 (2012)
  • Author(s): 向恵実
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 豊中
  • Year and Date: 2012-09-15
• [Presentation] カタユウレイボヤ幼生のGABA/グリシン作動性神経細胞の発生におけるSoxB1の役割 (2012)
  • Author(s): 森口大輔
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 豊中
  • Year and Date: 2012-09-15
• [Presentation] 同一シス転写調節領域によるメダカ錐体オプシン-miRNA遺伝子対の共発現機構 (2012)
  • Author(s): 大道裕
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 豊中
  • Year and Date: 2012-09-13
• [Presentation] メダカ視細胞特異的シス転写調節配列のin silico解析と実験検証 (2012)
  • Author(s): 浜西桜子
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 豊中
  • Year and Date: 2012-09-13
• [Presentation] メダカの後脳および脊髄に存在する新奇GnRHシステム (2012)
  • Author(s): 横田裕子
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 豊中
  • Year and Date: 2012-09-13
• [Presentation] ホヤ中枢神経系におけるPiwiのはたらき (2012)
  • Author(s): 島井光太郎
  • Organizer: ホヤ研究会2012 in京都
  • Place of Presentation: 京都
  • Year and Date: 2012-06-02
• [Presentation] カタユウレイボヤ幼生の眼外光受容細胞 (2012)
  • Author(s): 日下部岳広
  • Organizer: ホヤ研究会2012 in京都
  • Place of Presentation: 京都
  • Year and Date: 2012-06-02
• [Presentation] The miRNA-processing enzyme Dicer is required for morphogenesis of the Ciona intestinalis embryo (2012)
  • Author(s): Kana Waki
  • Organizer: Joint Meeting of the 45th Annual Meeting of the Japanese society of Developmental Biologists & the 64th Annual Meeting of the Japanese Society for Cell Biology
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-31
• [Presentation] A putative role of a Piwi protein in the central nervous system of Ciona intestinalis (2012)
  • Author(s): Kotaro Shimai
  • Organizer: Joint Meeting of the 45th Annual Meeting of the Japanese society of Developmental Biologists & the 64th Annual Meeting of the Japanese Society for Cell Biology
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-30
• [Presentation] Role of Hh signaling in tunicate development and chordate evolution (2012)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: Hedgehog 2012: Hedgehog Signaling in Development, Evolution and Disease
  • Place of Presentation: Singapore
  • Year and Date: 2012-03-20
• [Presentation] Role of Hh signaling in tunicate development and chordate evolution (2012)
  • Author(s): Takehiro G.Kusakabe
  • Organizer: Hedgehog 2012 : Hedgehog Signaling in Development, Evolution and Disease
  • Place of Presentation: BIOPOLIS, Singapore(招待講演)
  • Year and Date: 2012-03-20
• [Presentation] A novel GnRH system in the hindbrain and spinal cord of medaka Oryzias latipes (2012)
  • Author(s): Yuko Yokota
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都
• [Presentation] カタユウレイボヤ幼生のGABA/グリシン作動性神経細胞の発生におけるSoxB1 の役割 (2012)
  • Author(s): 森口大輔、西辻光希、島井光太郎、大道裕、一瀬葵、久原篤、安尾仁良、堀江健生、日下部岳広
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 豊中
• [Presentation] ホヤ中枢神経系におけるPiwiのはたらき (2012)
  • Author(s): 島井光太郎、堀江健生、白江-倉林麻貴、中村輝、日下部岳広
  • Organizer: ホヤ研究会2012 in Kyoto
  • Place of Presentation: 京都
• [Presentation] カタユウレイボヤ幼生の眼外光受容細胞 (2012)
  • Author(s): 日下部岳広、原真弓、杉内葉子、前田翔馬、中川将司
  • Organizer: ホヤ研究会2012 in Kyoto
  • Place of Presentation: 京都
• [Presentation] カタユウレイボヤにおける転写開始パターンとプロモーター構造および機能の網羅的解析 (2011)
  • Author(s): 横森類
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] ホヤのメチローム解析から探る組織特異的プロモーターメチル化の起源 (2011)
  • Author(s): 横山貴央
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] カタユウレイボヤにおける転写開始パターンとプロモーター構造および機能の網羅的解析 (2011)
  • Author(s): 横森類
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] ホヤのメチローム解析から探る組織特異的プロモーターメチル化の起源 (2011)
  • Author(s): 横山貴央
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] Regulation and specification mechanisms of glutamatergic neurons during development of the larval nervous system of the ascidian Ciona intestinalis (2011)
  • Author(s): Koki Nishitsuji
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] カタユウレイボヤ幼生のGABA/グリシン作動性神経細胞の細胞系譜と特異的転写調節機構の解析 (2011)
  • Author(s): 一瀬葵
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Novel GnRH-producing cells in the hindbrain and the spinal cord of vertebrates (2011)
  • Author(s): 瀧川徹
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-13
• [Presentation] Regulation and specification mechanisms of glutamatergic neurons during development of the larval nervous system of the ascidian Ciona intestinalis (2011)
  • Author(s): Koki Nishitsuji
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] カタユウレイボヤ幼生のGABA/グリシン作動性神経細胞の細胞系譜と特異的転写調節機構の解析 (2011)
  • Author(s): 一瀬葵
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] Novel GnRH-producing cells in the hindbrain and the spinal cord of vertebrates (2011)
  • Author(s): 瀧川徹
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-13
• [Presentation] カタユウレイボヤ中枢神経系のPiwiの働き (2011)
  • Author(s): 島井光太郎
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-22
• [Presentation] ホヤ幼生のGABA/グリシン作動性神経細胞の細胞系譜と細胞特異的転写制御機構の解析 (2011)
  • Author(s): 西辻光希
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-22
• [Presentation] ホヤ幼生のGABA/グリシン作動性神経細胞の細胞系譜と細胞特異的転写制御機構の解析 (2011)
  • Author(s): 西辻光希
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪アリーナ
  • Year and Date: 2011-09-22
• [Presentation] カタユウレイボヤ中枢神経系のPiwiの働き (2011)
  • Author(s): 島井光太郎
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪アリーナ
  • Year and Date: 2011-09-22
• [Presentation] ホヤ幼生のGABA/グリシン作動性神経細胞形成における転写因子SoxB1の役割 (2011)
  • Author(s): 一瀬葵
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川
  • Year and Date: 2011-09-21
• [Presentation] ホヤ幼生のGABA/グリシン作動性神経細胞形成における転写因子SoxB1の役割 (2011)
  • Author(s): 一瀬葵
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪アリーナ
  • Year and Date: 2011-09-21
• [Presentation] Are there GnRH-producing cells in the hindbrain and the spinal cord of vertebrates (2011)
  • Author(s): Toru Takigawa
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 静岡県三島市
  • Year and Date: 2011-09-08
• [Presentation] Bidirectionally transcribed cone opsin-miRNA gene pairs in the medaka Oryzias latipes (2011)
  • Author(s): Yutaka Daido
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 静岡県三島市
  • Year and Date: 2011-09-08
• [Presentation] Bidirectionally transcribed cone opsin-miRNA gene pairs in the medaka Oryzias latipes (2011)
  • Author(s): Yutaka Daido
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 東レ総合研修センター
  • Year and Date: 2011-09-08
• [Presentation] 脊索動物に脊椎動物の起源を探る (2011)
  • Author(s): 日下部岳広
  • Organizer: 日本進化学会第13回大会・シンポジウム「脳はどうやってうまれ,どうやって複雑化していったのか」
  • Place of Presentation: 京都
  • Year and Date: 2011-07-31
• [Presentation] 脊索動物に脊椎動物の起源を探る (2011)
  • Author(s): 日下部岳広
  • Organizer: 日本進化学会第13回大会
  • Place of Presentation: 京都大学百年記念ホール(招待講演)
  • Year and Date: 2011-07-31
• [Presentation] Deep evolutionary origin of non-reproductive GnRH in chordates (2011)
  • Author(s): Takehiro G.Kusakabe
  • Organizer: 6th International Tunicate Meeting
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2011-07-07
• [Presentation] A putative somatic role of the germline-specific gene piwi in the central nervous system of Ciona intestinalis (2011)
  • Author(s): Kotaro Shimai
  • Organizer: 6th International Tunicate Meeting
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2011-07-07
• [Presentation] Specification and differentiation of a GABAergic/glycinergic neuron subtype in the Ciona intestinalis larva (2011)
  • Author(s): Koki Nishitsuji
  • Organizer: 6th International Tunicate Meeting
  • Place of Presentation: Montreal, Canada
  • Year and Date: 2011-07-07
• [Presentation] The tunicate nervous system : insights into the origin of vertebrate-specific traits (2011)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: 第8回国際比較生理生化学会議(ICCPB2011) Symposium S31"Evolving shapes and development - viewed from changes in gene regulations"
  • Place of Presentation: 名古屋
  • Year and Date: 2011-06-05
• [Presentation] The tunicate nervous system : insights into the origin of vertebrate-specific traits (2011)
  • Author(s): Takehiro G.Kusakabe
  • Organizer: 第8回国際比較生理生化学会議
  • Place of Presentation: 名古屋国際会議場(招待講演)
  • Year and Date: 2011-06-05
• [Presentation] Towards understanding the role of cis-regulatory regions in evolution? (2011)
  • Author(s): Kenta Nakai
  • Organizer: 日本発生生物学会第44回大会
  • Place of Presentation: 沖縄コンベンションセンター(招待講演)
  • Year and Date: 2011-05-21
• [Presentation] 神経系の発生と多様性:脊索動物モデル・ホヤから脊椎動物モデル・メダカへの展開 (2011)
  • Author(s): 日下部岳広
  • Organizer: 第9回関西小型魚類勉強会
  • Place of Presentation: 独立行政法人産業総合技術研究所尼崎支所
  • Year and Date: 2011-05-12
• [Presentation] 神経系の発生と多様性:脊索動物モデル・ホヤから脊椎動物モデル・メダカへの展開 (2011)
  • Author(s): 日下部岳広
  • Organizer: 第9回関西小型魚類勉強会
  • Place of Presentation: 産業総合技術研究所、尼崎(招待講演)
  • Year and Date: 2011-05-12
• [Presentation] Sequence analyses of genetic and epigenetic information for transcriptional regulation (2011)
  • Author(s): Kenta Nakai
  • Organizer: The 9th Asia Pacific Bioinformatics Conference
  • Place of Presentation: Incheon, Korea
  • Year and Date: 2011-01-14
• [Presentation] Sequence Analyses of genetic and epigenetic information for transcriptional regulation (2011)
  • Author(s): Kenta Nakai
  • Organizer: The 9th Asia Pacific Bioinformatics Conference
  • Place of Presentation: Incheon, Korea(招待講演)
  • Year and Date: 2011-01-14
• [Presentation] Transcriptional regulation underlying the differentiation of glutamatergic neurons in the Ciona intestinalis larva (2011)
  • Author(s): Koki Nishitsuji
  • Organizer: International Symposium Genetic Regulation of Development : Commemorative of 27th International Prize for Biology
  • Place of Presentation: 京都
• [Presentation] Cell lineage and transcriptional regulation for a GABAergic/glycinergic neuron subtype in the ascidian larva (2011)
  • Author(s): Aoi Ichinose
  • Organizer: International Symposium Genetic Regulation of Development : Commemorative of 27th International Prize for Biology
  • Place of Presentation: 京都
• [Presentation] Bidirectionally transcribed cone opsin-miRNA gene pairs in the medaka Oryzias latipes (2011)
  • Author(s): Yutaka Daido
  • Organizer: International Symposium Genetic Regulation of Development : Commemorative of 27th International Prize for Biology
  • Place of Presentation: 京都
• [Presentation] Deep evolutionary origin of non-reproductive GnRH in chordates (2011)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: 6th International Tunicate Meeting
  • Place of Presentation: Montréal, Canada
• [Presentation] A putative somatic role of the germline-specific gene piwi in the central nervous system of Ciona intestinalis (2011)
  • Author(s): Kotaro Shimai
  • Organizer: 6th International Tunicate Meeting
  • Place of Presentation: Montréal, Canada
• [Presentation] Specification and differentiation of a GABAergic/glycinergic neuron subtype in the Ciona intestinalis larva (2011)
  • Author(s): Koki Nishitsuji
  • Organizer: 6th International Tunicate Meeting
  • Place of Presentation: Montréal, Canada
• [Presentation] Transcriptional regulation underlying the differentiation of glutamatergic neurons in the Ciona intestinalis larva (2011)
  • Author(s): Koki Nishitsuji
  • Organizer: International Symposium Genetic Regulation of Development
  • Place of Presentation: 京都ガーデンパレス
• [Presentation] Bidirectionally transcribed cone opsin-miRNA gene pairs in the medaka Oryzias latipes (2011)
  • Author(s): Yutaka Daido
  • Organizer: International Symposium Genetic Regulation of Development
  • Place of Presentation: 京都ガーデンパレス
• [Presentation] bHLH型転写因子Ptf1aはホヤ幼生においてドーパミン作動性ニューロンの分化に必須である (2010)
  • Author(s): 堀江健生
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] bHLH型転写因子Ptflaはホヤ幼生においてドーパミン作動性ニューロンの分化に必須である (2010)
  • Author(s): 堀江健生
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Current status of tunicate databases : DBTGR (2010)
  • Author(s): Takehiro G.Kusakabe
  • Organizer: 1st Tunicate Information System Meeting
  • Place of Presentation: Nice, France(招待講演)
  • Year and Date: 2010-11-12
• [Presentation] 転写因子SoxB1はホヤ幼生の尾部前端部抑制性ニューロンの発生に必要である (2010)
  • Author(s): 一瀬葵
  • Organizer: 日本動物学会第81回東京大会
  • Place of Presentation: 東京大学教養学部(駒場キャンパス)
  • Year and Date: 2010-09-23
• [Presentation] 転写因子SoxB1はホヤ幼生の尾部前端部抑制性ニューロンの発生に必要である (2010)
  • Author(s): 一瀬葵
  • Organizer: 日本動物学会第81回東京大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-09-23
• [Presentation] Transcriptional mechanisms underlying specification of neuron subtypes in the Ciona intestinalis larva (2010)
  • Author(s): Koki Nishitsuji
  • Organizer: 2nd Joint Meeting of The SFBD and JSDB 2010
  • Place of Presentation: Paris, France
  • Year and Date: 2010-05-26
• [Presentation] Current status of tunicate databases : DBTGR (2010)
  • Author(s): Takehiro G. Kusakabe
  • Organizer: 1st Tunicate Information System Meeting
  • Place of Presentation: Nice, France
• [Presentation] Transcriptional mechanisms underlying specification of neuron subtypes in the Ciona intestinalis larva (2010)
  • Author(s): Koki Nishitsuji
  • Organizer: 2nd Joint Meeting of The SFBD and JSDB 2010 - From Cells to Organs
  • Place of Presentation: Institut Pasteur–Paris, France
• [Book] Genome Structure, Functional Genomics, and Proteomics in Ascidians. Paul Denny, Chittaranjan Kole (Eds) Genome Mapping and Genomics in Laboratory Animals : Genome Mapping and Genomics in Animals (2012)
  • Author(s): Yasunori Sasakura, Nicolas Sierro, Kenta Nakai, Kazuo Inaba, and Takehiro G. Kusakabe
  • Total Pages: 261
  • Publisher: Springer-Verlag, Berlin Heidelberg
• [Book] Genome Structure, Functional Genomics, and Proteomics in Ascidians. In: Paul Denny, Chittaranjan Kole (Eds) Genome Mapping and Genomics in Laboratory Animals: Genome Mapping and Genomics in Animals, Vol. 4. (2012)
  • Author(s): Yasunori Sasakura, Nicolas Sierro, Kenta Nakai, Kazuo Inaba, Takehiro G. Kusakabe
  • Total Pages: 261
  • Publisher: Springer-Verlag, Berlin Heidelberg
• [Remarks] ホヤ遺伝子制御データベース(DBTGR)
  • URL: http://dbtgr.hgc.jp/
• [Remarks] ホヤ統合データベース(Aniseed)
  • URL: http://crfb.univ-mrs.fr/aniseed/
• [Remarks] DBTGR: Database of Tunicate Gene Regulation
  • URL: http://dbtgr.hgc.jp/
• [Remarks]
  • URL: http://dbtgr.hgc.jp/
• [Remarks]
  • URL: http://dbtgr.hgc.jp/