Elucidation of molecular mechanisms on potentiality of tumorigenesis in iPS cells by IVV method

• Project/Area Number: 22310121
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical genome science
• Research Institution: Keio University
• Principal Investigator: YANAGAWA Hiroshi 慶應義塾大学, 理工学部, 訪問教授 (40327672)
• Co-Investigator(Kenkyū-buntansha): TATEYAMA Seiji 慶應義塾大学, 理工学部, 特別研究講師 (90338202)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥20,410,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥4,710,000); Fiscal Year 2012: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2010: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: In vitrovirus / Klf4 / Lin28 / Nanog / iPS細胞 / mRNAディスプレイ / 分子機構 / 複合体 / Nap1 / EGFR / Trp2 / in vitro virus法 / Tnp2 / In vitro virus

Research Abstract

We comprehensively investigated protein-protein interactions (PPIs) of Lin28, Nanog,and Klf4 by mRNA display using the in vitrovirus (IVV) method. As a result, we identified Trp2 as a new Lin28-associated factor, Nap1 as a new Nanog-associated factor, and

Research Products

• [Journal Article] Application of mRNA display for in vitroselection of DNA-binding transcription factor complexes (2013)
  • Author(s): Tateyama,S.,Yanagawa,H
  • Journal Title: Methods Mol. Biol. Volume: 977 Pages: 95-109
  • DOI: 10.1007/978-1-62703-284-1_8
  • ISBN: 9781627032834, 9781627032841
  • Peer Reviewed
• [Journal Article] A phthalimide derivative that inhibits centrosomal clustering is effective on multiple myeloma (2012)
  • Author(s): Shiheido, H., Terada, F., Tabata, N., Hayakawa, I., Matsumura, N., Takashima, H., Ogawa, Y., Du,W., Yamada, T., Shoji, M., Sugai, T., Doi, N.,Iijima, S., Hattori, Y., Yanagawa, H.
  • Journal Title: PLoS ONE Volume: 7 Issue: 6 Pages: e38878-e38878
  • DOI: 10.1371/journal.pone.0038878
  • Peer Reviewed
• [Journal Article] An anilinoquinazoline derivative inhibits tumor growth through interaction with hCAP-G2, a subunit of condensin (2012)
  • Author(s): Shiheido, H., Y. Naito, H. Kimura, H. Genma, H. Takashima, T. Ono, T. Hirano, W. Du, T. Yamada, N. Doi, S. Iijima, Y. Hattori and H. Yanagawa
  • Journal Title: II. PLOS ONE Volume: 7(9) Issue: 9 Pages: e44889-e44889
  • DOI: 10.1371/journal.pone.0044889
  • Peer Reviewed
• [Journal Article] In vitro selection of Fab fragments by mRNA display and gene-linking emulsion PCR (2012)
  • Author(s): Sumida, T., Yanagawa, H., Doi, N.
  • Journal Title: J. Nucleic Acids Volume: 2012 Pages: 371379-371379
  • DOI: 10.1155/2012/371379
  • Peer Reviewed
• [Journal Article] A phthalimide derivative that inhibits centrosomal clustering is effective on multiple myeloma (2012)
  • Author(s): H.Shiheido, F.Terada, N.Tabata, I.Hayakawa, N.Matsumura, H.Takashima, Y.Ogawa, W.Du, T.Yamada, M.Shoji, T.Sugai, N.Doi, S.Iijima, Y.Hattori, H.Yanagawa
  • Journal Title: PLoS ONE Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] mRNA display selection of an optimized MDM2-binding peptide that potently inhibits MDM2-p53 interaction (2011)
  • Author(s): Shiheido, H., Takashima, H., Doi, N., Yanagawa, H.
  • Journal Title: PLoS ONE Volume: 6 Issue: 3 Pages: 1-9
  • DOI: 10.1371/journal.pone.0017898
  • Peer Reviewed
• [Journal Article] DNA display selection of peptide ligands for a full-length human G protein-coupled receptor on CHO-K1 cells (2011)
  • Author(s): Doi,N.,Yamakawa,N.,Matsumoto,H.,Yamamoto,Y.,Nagano,T.,Matsumura,N.,Horisawa,K.,Yanagawa,H.
  • Journal Title: PLoS ONE Volume: 7 Issue: 1 Pages: 1-8
  • DOI: 10.1371/journal.pone.0030084
  • Peer Reviewed
• [Journal Article] Development of IVV method into drug discovery (2011)
  • Author(s): Yanagawa, H
  • Journal Title: Drug Delivery System Volume: 26 Pages: 571-583
  • NAID: 130002100329
  • URL: http://square.umin.ac.jp/
  • Peer Reviewed
• [Journal Article] Protein complex prediction via verifying and reconstructing the topology of domain-domain interactions (2010)
  • Author(s): Ozawa, Y., Saito, R., Fujimori, S., Kashima, H.,Ishizaka, M.、Yanagawa, H., Miyamoto-Sato, E., Tomita, M.
  • Journal Title: BMC Bioinformatics Volume: 11 Issue: 1 Pages: 350-361
  • DOI: 10.1186/1471-2105-11-350
  • Peer Reviewed
• [Journal Article] Electrochemical detection of tyrosine derivatives and protein tyrosine kinase activity using boron-doped diamond electrode (2010)
  • Author(s): Chiku, M., Horisawa, K., Doi, N., Yanagawa, H., Einaga, Y.
  • Journal Title: Biosensors and Bioelectronics Volume: 9 Issue: 1 Pages: 235-240
  • DOI: 10.1016/j.bios.2010.06.027
  • Peer Reviewed
• [Journal Article] Operon structure optimization by random self-assembly (2010)
  • Author(s): Nakagawa, Y., Yugi,K., Tuge, K., Itaya, M., Yanagawa, H., Sakakibara, Y.
  • Journal Title: Natural Computing Volume: 9 Issue: 1 Pages: 173-181
  • DOI: 10.1007/s11047-009-9141-0
  • Peer Reviewed
• [Journal Article] Acrosome reaction-related steroidal saponin, Co-ARIS, from the starfish induces structural changes in microdomains (2010)
  • Author(s): Naruse, M., Suetomo, H., Matsubara, T., Sato, T., Yanagawa, H., Hoshi, M., Matsumoto, M.
  • Journal Title: Developmental Biology Volume: 347 Issue: 1 Pages: 47-153
  • DOI: 10.1016/j.ydbio.2010.08.019
  • Peer Reviewed
• [Journal Article] Comparative characterization of random-sequence proteins consiting of 5, 12, and 20 kinds of amino acids (2010)
  • Author(s): Tanaka,J., Doi, N., Takashima, H., Yanagawa, H.
  • Journal Title: Protein Sci. Volume: 19 Issue: 4 Pages: 235-240
  • DOI: 10.1002/pro.358
  • Peer Reviewed
• [Journal Article] The Musashi family RNA-binding proteins in stem cells (2010)
  • Author(s): Horisawa, K., Imai, T., Okano, H., Yanagawa, H.
  • Journal Title: Biomolcular Concepts Volume: 1 Issue: 1 Pages: 59-66
  • DOI: 10.1515/bmc.2010.005
  • Peer Reviewed
• [Journal Article] Electrochemical detection of tyrosine derivatives and protein tyrosine kinase activity using boron-doped diamond electrode (2010)
  • Author(s): M.Chiku, K.Horisawa, N.Doi, H.Yanagawa, Y.Einaga
  • Journal Title: Biosensors and Bioelectronics Volume: 26 Pages: 235-240
  • Peer Reviewed
• [Journal Article] Acrosome reaction-related steroidal saponin, Co-ARIS, from the starfish induces structural changes in microdomains (2010)
  • Author(s): M.Naruse, H.Suetomo, T.Matsubara, T.Sato, H.Yanagawa, M.Hoshi, M.Matsumoto
  • Journal Title: Developmental Biology Volume: 347 Pages: 147-153
  • Peer Reviewed
• [Journal Article] Comparative characterization of random-sequence proteins consiting of 5, 12, and 20 kinds of amino acids (2010)
  • Author(s): J.Tanaka, N.Doi, H.Takashima, H.Yanagawa
  • Journal Title: Protein Science Volume: 19 Pages: 235-240
  • Peer Reviewed
• [Journal Article] mRNA display selection of an optimized MDM2-binding peptide that potently inhibits MDM2-p53 interaction (2010)
  • Author(s): H.Shiheido, H.Takashima, N.Doi, H.Yanagawa
  • Journal Title: PLoS ONE Volume: 6
  • Peer Reviewed
• [Journal Article] The Musashi family RNA-binding proteins in stem cells (2010)
  • Author(s): K.Horisawa, T.Imai, H.Okano, H.Yanagawa
  • Journal Title: Biomolcular Concepts Pages: 59-66
  • Peer Reviewed
• [Presentation] Target identification of antitumor drugs against intractable tumors by mRNA display (2012)
  • Author(s): Shiheido, H., Terada, F., Naito, Y., Tabata, N., Kimura, H., Hayakawa, I., Genma, H., Matsumura, N.,Takashima, H., Tokunaga, M., Ogawa, Y., Ono, T., Hirano, T., Du, W., Yamada, T., Shoji, M., Sugai, T., Doi, N., Iijima, S., Hattori, Y., Yanagawa, H
  • Organizer: 35th MBSJ 2012
  • Place of Presentation: Fukuoka
  • Year and Date: 2012-12-13
• [Presentation] An anilinoquinazoline derivative Q15 induces apoptosis through interacting with MIP-2A (2012)
  • Author(s): Tokunaga, M,, Shiheido, H., Tabata, N., Sakuma, Y., Takashima, H., Horisawa, K., Doi, N., Yanagawa, H
  • Organizer: 35th MBSJ 2012
  • Place of Presentation: Fukuoka
  • Year and Date: 2012-12-13
• [Presentation] Screening of natural products against induced cancer stem cells (2012)
  • Author(s): Suzuki, H., Tabata, N., Sakuma, Y., Yoshimura, Y.,Tsuji, T., Shoji, M., Sugai, T., Shimizu, T., Saya, H., Doi,N., Yanagawa, H
  • Organizer: 35th MBSJ 2012
  • Place of Presentation: Fukuoka
  • Year and Date: 2012-12-13
• [Presentation] Exploration and characterization of a novel target gene for an iPS cell inducible factor (2012)
  • Author(s): Ota, K., Tateyama, S., Horisawa, K., Doi, N., Yanagawa, H
  • Organizer: KLF4, 35th MBSJ
  • Place of Presentation: Fukuoka
  • Year and Date: 2012-12-13
• [Presentation] タンパク質の相互作用からゲノム創薬への展開 (2012)
  • Author(s): 柳川 弘志
  • Organizer: 慶應義塾大学理工学部生命情報学科10周年記念シンポジウム
  • Place of Presentation: 横浜
  • Year and Date: 2012-04-28
• [Presentation] In vitroevolution of high-affinity anti-CCR5 single chain Fv via neutral drift libraries (2011)
  • Author(s): Sato, R., Doi,N., Mitsui,, S., Shoji,S., Matsushima, K., Yokochi, S., Yanagawa, H
  • Organizer: 34thMBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-13
• [Presentation] FK506 inhibits the function of hereditary spastic paraplegia protein spartin (2011)
  • Author(s): Tokunaga,M.,Shiheido,H.,Hayakawa, I., Takashima, H., Yanagawa, H
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-13
• [Presentation] In vitroselection of anti-CD44vsc Fv inhibiting CD44v-xCT interaction (2011)
  • Author(s): Umezawa,H., Tabata,N., Sakuma,Y., Takenaka,Y., Matsumoto,Y., Doi,N., Nagano, O., Saya, H., Yanagawa, H
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-13
• [Presentation] Screening of anilinoquinazoline derivatives inhibiting migration and invasion of induced cancer stem cells and their action mechanism (2011)
  • Author(s): Tabata,N., Sakuma, Y., Shimizu, T., Saya, H., Ogawa,Y., Shoji, M., Sugai, T., Doi, N., Yanagawa, H
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-13
• [Presentation] In vitro virus (IVV) method revealed an interaction between CD44 and pyruvate kinase M2 (PKM2) (2011)
  • Author(s): Tateyama,S., Nagano,O., Tamada,M., Saya,H., Doi,N., Yanagawa, H
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-13
• [Presentation] In vitro selection of anti-CD44v scFv inhibiting CD44v-xCT interaction (2011)
  • Author(s): H.Umezawa, N.Tabata, Y.Sakuma, Y.Takenaka, Y.Matsumoto, N.Doi, O.Nagano, H.Saya, H.Yanagawa
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-13
• [Presentation] In vitro evolution of high-affinity anti-CCR5 single chain Fvvia neutral drift libraries (2011)
  • Author(s): R.Sato, N.Doi, S.Mitsui,, S.Shoji, K.Matsushima, S.Yokochi, H.Yanagawa
  • Organizer: 34th MBSJ 2011
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-13
• [Presentation] In vitroselection of a novel FK506-binding protein by using mRNA display (2011)
  • Author(s): Tokunaga, M., Shiheido, H., Hayakawa,I., Utsumi, A., Takashima,H., Doi,N., Horisawa,K., Yanagawa, H
  • Organizer: EMBO Molecular Medicine Conference: Molecular Insights for Innovative Therapies
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2011-12-02
• [Presentation] In vitro selection of a novel FK506-binding protein by using mRNA display (2011)
  • Author(s): M.Tokunaga, H.Shiheido, I.Hayakawa, A.Utsumi, H.Takashima, N.Doi, K.Horisawa, H.Yanagawa
  • Organizer: EMBO Molecular Medicine Conference : Molecular Insights for Innovative Therapies
  • Place of Presentation: Heidelberg, Germany
  • Year and Date: 2011-12-02
• [Presentation] Molecular interaction network of KLF4 using mRNA display (2010)
  • Author(s): Tateyama, S., Kodama, T., Ohta, K., Yanagawa, H.
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, U.S.A
  • Year and Date: 2010-12-18
• [Presentation] Molecular interaction network of KLF4 using mRNA display (2010)
  • Author(s): Seiji Tateyama, Tatsushi Kodama, Kiyoto Ohta, Hiroshi Yanagawa
  • Organizer: The 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Honolulu, Hawaii
  • Year and Date: 2010-12-18
• [Presentation] In vitrovirus (IVV)法を用いたCD44を介した遺伝子発現制御機構の解析 (2010)
  • Author(s): 舘山誠司、永 野 修 、佐 谷 秀 行 、柳川弘志
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-12
• [Presentation] In vitrovirus法によるLin28相互作用因子の解析 (2010)
  • Author(s): 下門大祐、舘山誠司、土居信英、柳川弘志
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-12
• [Presentation] In vitro Virus(IVV)法を用いたCD44を介した遺伝子発現制御機構の解析 (2010)
  • Author(s): 舘山誠司, 永野修, 佐谷秀行, 柳川弘志
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会(BMB2010)
  • Place of Presentation: 神戸ポートアイランド、神戸
  • Year and Date: 2010-12-12
• [Presentation] In vitro Virus法によるLin28相互作用因子の解析 (2010)
  • Author(s): 下門大祐, 舘山誠司, 士居信英, 柳川弘志
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会(BMB2010)
  • Place of Presentation: 神戸ポートアイランド、神戸
  • Year and Date: 2010-12-12
• [Presentation] ハイリスク骨髄腫克服のためのスクリーニングシステムの構築 (2010)
  • Author(s): 鈴木裕也、寺田蕗子、内藤悠平、柳川弘志、杜ぶん林、山田健人、土居信英、飯島史朗、松下麻衣子、田原佳代子、服部豊
  • Organizer: 第54回日本薬学会関東支部大会
  • Place of Presentation: 八王子
  • Year and Date: 2010-10-02
• [Presentation] ハイリスク骨髄腫克服のためのスクリーニングシステムの構築 (2010)
  • Author(s): 鈴木裕也、寺田蕗子、内藤悠平、柳川弘志、杜ぶん林、山田健人、土居信英、飯島史朗、松下麻衣子、田原佳代子、服部豊
  • Organizer: 第54回日本薬学会関東支部大会
  • Place of Presentation: 東京薬科大学、八王子
  • Year and Date: 2010-10-02
• [Presentation] mRNAディスプレイ法により選択されたMDM2結合ペプチドはMDM2-p53相互作用を強力に阻害する (2010)
  • Author(s): 始平堂弘和、土居信英、柳川弘志
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-25
• [Presentation] 新規アニリノキナゾリン誘導体を用いたin vivo におけるハイリスク多発性骨髄腫の治療効果 (2010)
  • Author(s): 内藤悠平、鈴木裕也、尾崎由枝、柳川弘志, 杜ぶん林、山田健人、土居信英、飯島史朗、松下麻衣子、田原佳代子、服部豊
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-09-25
• [Presentation] 新規アニリノキナゾリン誘導体を用いたin vivoにおけるハイリスク多発性骨髄腫の治療効果 (2010)
  • Author(s): 内藤悠平、鈴木裕也、尾崎由枝、柳川弘志, 杜ぶん林、山田健人、土居信英、飯島史朗、松下麻衣子、田原佳代子、服部豊
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場・リーガロイヤルホテル、大阪
  • Year and Date: 2010-09-25
• [Presentation] mRNAディスプレイ法により選択されたMDM2結合ペプチドはMDM2-p53相互作用を強力に阻害する (2010)
  • Author(s): 始平堂弘和、土居信英、柳川弘志
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪国際会議場・リーガロイヤルホテル、大阪
  • Year and Date: 2010-09-24
• [Presentation] In vitrovirus(IVV)法を用いた次世代タンパク質機能解析システム (2010)
  • Author(s): 柳川弘志
  • Organizer: 第10回日本蛋白質科学会年会シンポジウム「蛋白質研究法:先進計測・解析技術の新展開」
  • Place of Presentation: 札幌
  • Year and Date: 2010-06-16
• [Presentation] In vitro virus(IVV)法を用いた次世代タンパク質機能解析システム (2010)
  • Author(s): 柳川弘志
  • Organizer: 第10回日本蛋白質科学会年会シンポジウム「蛋白質研究法:先進計測・解析技術の新展開」
  • Place of Presentation: 札幌コンベンションセンター、札幌(招待講演)
  • Year and Date: 2010-06-16
• [Presentation] タンパク質の相互作用からゲノム創薬への展開
  • Author(s): 柳川 弘志
  • Organizer: 慶應義塾大学理工学部生命情報学科１０周年記念シンポジウム
  • Place of Presentation: 横浜
  • Invited
• [Presentation] arget identification of antitumor drugs against intractable tumors by mRNA display
  • Author(s): Shiheido H, Terada F, Naito Y, Tabata N, Kimura H, Hayakawa I, Genma H, Matsumura N, Takashima H, Tokunaga M, et al.
  • Organizer: 35th MBSJ 2012
  • Place of Presentation: Fukuoka
• [Presentation] An anilinoquinazoline derivative Q15 induces apoptosis through interacting with MIP-2A
  • Author(s): Tokunaga M, Shiheido H, Tabata N, Sakuma Y, Takashima H, Horisawa K, Doi N, Yanagawa H
  • Organizer: 35th MBSJ 2012
  • Place of Presentation: Fukuoka
• [Presentation] Screening of natural products against induced cancer stem cells
  • Author(s): Suzuki H, Tabata N, Sakuma Y, Yoshimura Y, Tsuji T, Shoji M, Sugai T, Shimizu T, Saya H, Doi N, Yanagawa H
  • Organizer: 35th MBSJ 2012
  • Place of Presentation: Fukuoka
• [Presentation] Exploration and characterization of a novel target gene for an iPS cell inducible factor, KLF4
  • Author(s): Ota K, Tateyama S, Horisawa K, Doi N, Yanagawa H
  • Organizer: 35th MBSJ 2012
  • Place of Presentation: Fukuoka
• [Book] Evolutionary Engineering of Artificial Proteins with Limited Sets of Primitive Amino Acids (2012)
  • Author(s): Tanaka,J., Yanagawa,H., Doi,N. InTech
  • Publisher: Protein Engineering
• [Book] Useful method to search for DNA-binding transcription factor complexesusing mRNA (2012)
  • Author(s): Tateyama S, Yanagawa H
  • Publisher: Methods in Molecular Biology
• [Book] Application of mRNA display for in vitro selection of DNA-binding transcription factor complexes. IN: Methods in Molecular Biology: Gene Regulation (2012)
  • Author(s): Tateyama S. and Yanagawa H
  • Publisher: Humana Press in brand of Springer
• [Book] The Musashi proteins in neural stem cell/progenitor cells (2011)
  • Author(s): Horisawa,K., Yanagawa, H
  • Publisher: Neural Stem Cells and Therapy
• [Book] John Wiley & Sons Ltd (2011)
  • Author(s): Doi,N.,Kakukawa,K.,Oishi,Y.,Yanagawa,H
  • Publisher: Chemical Synthetic Biology
• [Book] Neural Stem Cells and Therapy (2011)
  • Author(s): K.Horisawa, H.Yanagawa
  • Publisher: InTech
• [Book] Chemical Synthetic Biology (2011)
  • Author(s): N.Doi, K.Kakukawa, Y.Oishi, H.Yanagawa
  • Publisher: John Wiley & Sons Ltd
• [Book] 生体高分子の進化工学、現代生物科学入門第9巻:合成生物学 (2010)
  • Author(s): 柳川弘志、土居信英
  • Publisher: 岩波書店
• [Book] Newton(ニュートン): 無生物から生物ができる-生命誕生の不思議 (2010)
  • Author(s): 柳川弘志
  • Publisher: 教育社
• [Book] 生体高分子の進化工学、現代生物科学入門第9:合成生物学 (2010)
  • Author(s): 柳川弘志、土居信英
  • Total Pages: 34
  • Publisher: 岩波書店
• [Book] Newton(ニュートン):無生物から生物ができる-生命誕生の不思議 (2010)
  • Author(s): 柳川弘志
  • Total Pages: 32
  • Publisher: 教育社