| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2012: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2011: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
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| Research Abstract |
In order to reconstruct the nuclear genome of Arabidopsis thaliana, we introduced the LoxP sequence, which originated from bacteriophase P1, into the chromosomes, and have created 30 lines carrying one or two copies of LoxP per chromosome. These LoxP sites were combined in the genome by crossing, and the sequence-specific recombination was induced with the Cre-recombinase. PCR and FISH analyses revealed that reciprocal translocations are possibly induced by recombination between two LoxP sites located on different chromosomes. It was also suggested that alteration of chromatin structure associated with the reciprocal translocation, induces phenotypic changes.
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