Regulation of sodium and glycine betaine in a halotolerant cyanobacterium Aphanothece halophytica

• Project/Area Number: 22310131
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied genomics
• Research Institution: Meijo University
• Principal Investigator: TAKABE Teruhiro 名城大学, 総合研究所, 教授 (80097766)
• Co-Investigator(Kenkyū-buntansha): TANAKA Yoshito 名城大学, 理工学部, 教授 (10247679)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000); Fiscal Year 2013: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: 耐塩性ラン藻 / グリシンベタイン / 塩ストレス / 環境 / 食糧 / 植物 / 遺伝子工学 / 浸透圧

Research Abstract

Molecular mechanisms of regulations for sodium ion and osmoprotect glycinebetaine were studied using a halotolerant cyanobacterium from Dead Sea. F-type Na+-ATPase, sunscreen molecules were identified. Importance of PhoD alkaline phosphatase, amino acid transporters and precursers of glycine were examined.

Research Products

• [Journal Article] Identification and upregulation of biosynthetic genes required for accumulation of mycosporine-2-glycine under salt stress conditions in the halotolerant cyanobacterium Aphanothece halophytica (2014)
  • Author(s): Waditee-Sirisattha R, Kageyama H, Sopun W, Tanaka Y, Takabe T
  • Journal Title: Appll Environ Microbiol Volume: 80 Pages: 1763-1769
  • Peer Reviewed
• [Journal Article] Expression and functional analysis of putative vacuolar Ca^<2+>-transporters (CAXs and ACAs) in roots of salt tolerant and sensitive rice cultivars (2014)
  • Author(s): Yamada N, Theerawitaya C, Cha-um S, Kirdmane C, Takabe T
  • Journal Title: Protoplasma Volume: (in press)
  • Peer Reviewed
• [Journal Article] Identification and upregulation of biosynthetic genes required for accumulation of mycosporine-2-glycine under salt stress conditions in the halotolerant cyanobacterium Aphanothece halophytica (2014)
  • Author(s): R. Waditee-Sirisattha, H. Kageyama, W. Sopun, Y. Tanaka, T. Takabe
  • Journal Title: AEM Volume: 80 Issue: 5 Pages: 1763-1769
  • DOI: 10.1128/aem.03729-13
  • Peer Reviewed
• [Journal Article] Expression and functional analysis of putative vacuolar Ca2+-transporters (CAXs and ACAs) in roots of salt tolerant and sensitive rice cultivars (2014)
  • Author(s): N. Yamada, C. Theerawitaya, S. Cha-um, C. Kirdmane, T. Takabe:
  • Journal Title: Protoplasma Volume: 251 Issue: 5 Pages: 1-9
  • DOI: 10.1007/s00709-014-0615-2
  • Peer Reviewed
• [Journal Article] Physiological, biochemical and molecular responses of the halophilic cyanobacterium Aphanothece halophytica to Pi-deficiency (2013)
  • Author(s): Tripathi K, Kageyama H, Takabe T, Rai AK
  • Journal Title: Eur J Phycology Volume: 48 Pages: 461-473
  • Peer Reviewed
• [Journal Article] Physiological features and growth characters of oil palm (Elaeis guineensis Jacq.) in response to reduced water-deficit and rewatering (2013)
  • Author(s): Cha-um S, Yamada N, Takabe T, Kirdmanee C
  • Journal Title: Aust J Crop Sci Volume: 7 Pages: 432-439
  • Peer Reviewed
• [Journal Article] Regulation of some carbohydrate metabolism-related genes, starch and soluble sugar contents, photosynthetic activities and yield attributes of two contrasting rice genotypes subjected to salt stress (2013)
  • Author(s): Thanaphol Boriboonkaset, Cattarin Theerawitaya, Nana Yamada, Aussanee Pichakum, Kanyaratt Supaibulwatana, Suriyan Cha-um, Teruhiro Takabe, Chalermpol Kirdmanee
  • Journal Title: Protoplasma Volume: 250 Issue: 5 Pages: 1157-1167
  • DOI: 10.1007/s00709-013-0496-9
  • Peer Reviewed
• [Journal Article] Physiological, biochemical and molecular responses of the halophilic cyanobacterium Aphanothece halophytica to Pi-deficiency (2013)
  • Author(s): K. Tripathi, NK. Sharma, H. Kageyama, T. Takabe, AK. Rai
  • Journal Title: Eur J Phycol Volume: 48 Issue: 4 Pages: 461-473
  • DOI: 10.1080/09670262.2013.859303
  • Peer Reviewed
• [Journal Article] Sodium-dependent uptake of glutamate by novel ApGltS enhanced growth under salt stress of halotolerant cyanobacterium Aphanothece halophytica (2012)
  • Author(s): Boonburapong B, Laloknam S, Yamada N, Incharoensakdi A, Takabe T
  • Journal Title: Biosci Biotechnol Biochem Volume: 76 Pages: 1702-1707
  • NAID: 10031123175
  • Peer Reviewed
• [Journal Article] Anabaena sp. PCC7120 transformed with glycine methylation genes from Aphanothece halophytica synthesized glycine betaine showing increased tolerance to salt (2012)
  • Author(s): Waditee-Sirisattha R, Singh M, Kageyama H, Sittipol D, Rai AK, Takabe T
  • Journal Title: Arch Microbiol Volume: 194 Pages: 909-914
  • Peer Reviewed
• [Journal Article] Overexpression of serine hydroxymethyltransferase from halotolerant cyanobacterium in Escherichia coli results in increased accumulation of choline precursors and enhanced salinity tolerance (2012)
  • Author(s): Waditee-Sirisattha R, Sittipol D, Tanaka Y, Takabe T
  • Journal Title: FEMS Microbiol Lett Volume: 333 Pages: 46-53
  • Peer Reviewed
• [Journal Article] Overexpression of serine hydroxymethyltransferase from halotolerant cyanobacterium in Escherichia coli results in increased accumulation of choline precursors and enhanced salinity tolerance (2012)
  • Author(s): Rungaroon Waditee-Sirisattha, Daungjai Sittipol, Yoshito Tanaka, Teruhiro Takabe
  • Journal Title: FEMS Microbiol Lett. Volume: 333 Issue: 1 Pages: 46-53
  • DOI: 10.1111/j.1574-6968.2012.02597.x
  • Peer Reviewed
• [Journal Article] Anabeana sp. PCC7120 transformed with glycine methylation genes from Aphanothece halophytica synthesized glycine betaine showing increased tolerance to salt (2012)
  • Author(s): Rungaroon Waditee-Sirisattha, Meenakshi Singh, Hakuto Kageyama, Daungjai Sittipo, Ashwani K. Rai, Teruhiro Takabe
  • Journal Title: Arch. Microbiol. Volume: 194 Issue: 11 Pages: 909-914
  • DOI: 10.1007/s00203-012-0824-z
  • Peer Reviewed
• [Journal Article] Sodium-Dependent Uptake of Glutamate by Novel ApGltS Enhanced Growth under Salt Stress of Halotolerant Cyanobacterium <i>Aphanothece halophytica</i> (2012)
  • Author(s): Boonburapong B, Laloknam S, Yamada N, Incharoensakdi A, Takabe T.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 9 Pages: 1702-1707
  • DOI: 10.1271/bbb.120309
  • NAID: 130004137965
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Physio-Biochemical Responses of Oil Palm (<i>Elaeis guineensis</i> Jacq.) Seedlings to Mannitol- and Polyethylene Glycol-Induced Iso-Osmotic Stresses (2012)
  • Author(s): Suriyan Cha-um, Teruhiro Takabe, Chalermpol Kirdmanee
  • Journal Title: Plant Production Science Volume: 15 Issue: 2 Pages: 65-72
  • DOI: 10.1626/pps.15.65
  • NAID: 130004462263
  • ISSN: 1343-943X, 1349-1008
  • Peer Reviewed
• [Journal Article] An alkaline phosphatase/phosphodiesterase, PhoD, induced by salt stress and secreted out of the cells of Aphanothece halophytica, a halotolerant cyanobacterium (2011)
  • Author(s): Kageyama H, Tripathi K, Rai AK, Cha-Um S, Waditee-Sirisattha R, Takabe T
  • Journal Title: Appl Environ Microbiol Volume: 77 Pages: 5178-5183
  • Peer Reviewed
• [Journal Article] The Arabidopsis aminopeptidase LAP2 regulates plant growth, leaf longevity and stress response (2011)
  • Author(s): Waditee-Sirisattha R, Shibato J, Rakwal R, Sirisattha S, Hattori A, Nakano T, Takabe T, Tsujimoto M
  • Journal Title: New Phytol Volume: 191 Pages: 958-969
  • Peer Reviewed
• [Journal Article] Expression and substrate specificity of betaine/proline transporters suggest a novel choline transport mechanism in sugar beet (2011)
  • Author(s): Yamada N, Sakakibara S, Tsutsumi K, Waditee R, Tanaka Y, Takabe T
  • Journal Title: J Plant Physiol Volume: 168 Pages: 1609-1616
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Yamada N, Cha-Um S, Kageyama H, Promden W, Tanaka Y, Kirdmanee C, Takabe T
  • Journal Title: Tree Physiol Volume: 31 Pages: 462-468
  • Peer Reviewed
• [Journal Article] alotolerant cyanobacterium Aphanothece halophytica contains an Na^+dependent F_1F_0-ATP synthase with a potential role in salt-stress tolerance (2011)
  • Author(s): Soontharapirakkul K, Promden W, Yamada N, Kageyama H, Incharoensakdi A, Iwamoto-Kihara A, Takabe T
  • Journal Title: J Biol Chem Volume: 286 Pages: 10169-10176
  • Peer Reviewed
• [Journal Article] Halotolerant Cyanobacterium Aphanothece halophytica Contains an Na^+-dependent F_1F_0-ATP Synthase with a Potential Role in Salt-Stress Tolerance (2011)
  • Author(s): Soontharapirakkul K, Promden W, Yamada N, Kageyama H, Incharoensakdi A, Iwamoto-Kihara A, Takabe T
  • Journal Title: J Biol Chem Volume: 286 Issue: 12 Pages: 10169-10176
  • DOI: 10.1074/jbc.m110.208892
  • Peer Reviewed
• [Journal Article] Halotolerant Cyanobacterium Aphanothece halophytica Contains an Na^+-dependent F_1F_0-ATP Synthase with a Potential Role in Salt-stress Tolerance. (2011)
  • Author(s): Soontharapirakkul K, Promden W, Yamada N, Kageyama H, Incharoensakdi A, Iwamoto-Kihara A, Takabe T.
  • Journal Title: J Biol Chem Volume: 286 Pages: 10169-10176
  • Peer Reviewed
• [Journal Article] Glutamate 85 is involved in the sodium/proton exchange activity of the Escherichia coli ChaA. (2010)
  • Author(s): Fukaya F, Tanaka K, Waditee R, Tanaka Y, Nakamura T, Takabe T.
  • Journal Title: Biosci Biotechnol Biochem Volume: 74 Pages: 1116-1119
  • Peer Reviewed
• [Journal Article] Coherent dynamics and ultrafast excited state relaxation of blue copper protein ; plastocyanin. (2010)
  • Author(s): Nagasawa Y, Fujita K, Katayama T, Ishibashi Y, Miyasaka H, Takabe T, Nagao S, Hirota S.
  • Journal Title: Phys Chem Chem Phys Volume: 166 Pages: 6067-6075
  • Peer Reviewed
• [Journal Article] Ion contents, relative electrolyte leakage, praline accumulation, photosynthetic abilities and growth characteristics of oil palm seedlings in response to salt stress. (2010)
  • Author(s): Suriyan Cha-um, T., Takabe, T., Kirdmanee C
  • Journal Title: Pak.J.Boti. Volume: 42 Pages: 2191-2020
  • Peer Reviewed
• [Presentation] 耐塩性シアノバクテリアにおけるマイコスポリン様アミノ酸合成経路の塩ストレス応答 (2014)
  • Author(s): 景山伯春，Rungaroon WADITEE-SIRISATTHA，Warangkana SOPUN，田中義人，高倍昭洋
  • Organizer: 第５５回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] シュガービートのセリン合成酵素遺伝子の塩ストレス応答 (2014)
  • Author(s): 田中義人、山田奈々、柴田卓弥、高橋弘之、高倍鉄子、高倍昭洋
  • Organizer: 第５５回日本植物生理学会年会
  • Place of Presentation: 富山
• [Presentation] Metabolic Engineering for Stress Tolerance in Cyanobacteria and Microalgae (2012)
  • Author(s): Teruhiro Takabe
  • Organizer: 2nd Asia-Oceania Algae Innovation Summit:“Algae for Sustainable Development
  • Place of Presentation: Bangkok, Thailand
  • Invited
• [Presentation] Role of F-type Na^+-ATPase for salt tolerance (2011)
  • Author(s): Teruhiro Takabe
  • Organizer: International Symposium on Green Biotechnology
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2011-10-20
• [Presentation] Regulation of DMSPin Ulva (2011)
  • Author(s): Shibata Ayumi, Minoru Fukaya, Hakuto Kageyama, Teruhiro Takabe
  • Organizer: International Symposium on Green Biotechnology
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 2011-10-20
• [Presentation] Sodium Ion Homeostasis in Halotolerant Cyanobacterium---Toward Saline Green Biotechnology for Food, Energy, and Global Environment--- (2011)
  • Author(s): Teruhiro Takabe
  • Organizer: Royal Golden Jubilee Ph.D.Congress XII
  • Place of Presentation: Chonburi, Thailand
• [Presentation] シュガービートにおけるベタイン/プロリントランスポーターの組織特異的な発現とコリン輸送について (2011)
  • Author(s): 山田奈々、田中義人、高倍昭洋
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] Metabolic Engineering of Osmoprotectant Glycine Betaine (2010)
  • Author(s): Teruhiro Takabe
  • Organizer: Special seminar of Directorate of Rice Research(ICAR)
  • Place of Presentation: Hyderabard India
  • Year and Date: 2010-09-09
• [Presentation] Metabolic Engineering of Osmoprotectant Glycine Betaine---Toward Abiotic Stress Tolerant Plants for Food, Energy, and Global Environment--- (2010)
  • Author(s): Teruhiro Takabe
  • Organizer: Royal Golden Jubilee Ph.D.Congress XI
  • Place of Presentation: Chonburi, Thailand