Screening of natural products which inhibits a new menaquinone biosynthetic pathway
Project/Area Number |
22310139
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Living organism molecular science
|
Research Institution | Hokkaido University |
Principal Investigator |
DAIRI Tohru 北海道大学, 大学院・工学研究院, 教授 (70264679)
|
Project Period (FY) |
2010 – 2012
|
Project Status |
Completed (Fiscal Year 2012)
|
Budget Amount *help |
¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
|
Keywords | 微生物 / メナキノン / 生合成 / フタロシン経路 / 阻害剤 / フタロシン / ピロリ菌 / Radical SAM / futalosine hydrolase / futalosine / aminodeoxy futalosine / inhibitor |
Research Abstract |
The futalosine pathway is operating in some bacteria for the biosynthesis of menaquinone. Futalosine is converted into dehypoxanthinyl futalosine (DHFL) by MqnB.In this study, three routes to the formation of DHFL were suggested. DHFL may have been directly formed by MqnB in Thermus thermophilus. In Streptomyces coelicolor, aminodeoxy FL (AFL) was converted to FL by deaminases, then to DHFL. In contrast, MqnB of Helicobacter pylori directly converted AFL into DHFL. To identify compounds that specifically inhibit the futalosin pathway, we used B. halodurans that has FL pathway as a test strain. Finally, two candidate fungal culture broths were obtained.
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Report
(4 results)
Research Products
(48 results)
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[Journal Article] Reveromycin A biosynthesis uses RevG and RevJ for stereospecific spiroacetal formation2011
Author(s)
S. Takahashi, A. Toyoda, Y. Sekiyama, H.Takagi, T. Nogawa, M. Uramoto, R. Suzuki, H. Koshino, T. Kumano, S. Panthee, T. Dairi, J. Ishikawa, H. Ikeda, Y. Sakaki, and H. Osada
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Journal Title
Nat. Chem. Biol.
Volume: 7
Pages: 461-468
Related Report
Peer Reviewed
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[Journal Article] Dairi, Branched fatty acids inhibit the biosynthesis of menaquinone in Helicobacter pylori.2011
Author(s)
R. Tanaka, T. Kunisada, N. Kushida, K.Yamada, S. Ikeda, M. Noike, Y. Ono, N. Itoh, H. Takami, H. Seto, and T.
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Journal Title
J. Antibiot.
Volume: 64
Pages: 151-153
Related Report
Peer Reviewed
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[Journal Article] Branched fatty acids inhibit the biosynthesis of menaquinone in Helicobacter pylori.2011
Author(s)
R.Tanaka, T.Kunisada, N.Kushida, K.Yamada, S.Ikeda, M.Noike, Y.Ono, N.Itoh, H.Takami, H.Seto, T.Dairi
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Journal Title
J.Antibiot.
Volume: 64
Pages: 151-153
NAID
Related Report
Peer Reviewed
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