Function and structure of novel glycolipid essential for membrane protein integration
| Project/Area Number |
22310142
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Suntory Foundation for Life Sciences |
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Principal Investigator |
SHIMAMOTO Keiko 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主幹研究員 (70235638)
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| Co-Investigator(Renkei-kenkyūsha) |
NISHIYAMA Ken-ichi 岩手大学, 農学部, 教授 (80291334)
MIURA Kaoru (NOMURA Kaoru) 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 研究員 (90353515)
KUSUMOTO Shoichi 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 元所長 (30028253)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
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| Keywords | 糖脂質 / 膜タンパク質 / 糖鎖 / 生体膜 / 膜挿入 / 構造決定 / 有機合成 / 生理活性物質 / 酵素 / シャペロン / 生理活性機構 / 生活性物質 |
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| Research Abstract |
We have found a novel integration factor in the inner membrane of E. coli, naming it MPIase (membrane protein integrase). MPIase turned out to be a glycolipid composed of diacylglycerol and a glycan chain of three acetylated aminosugars linked through pyrophosphate. Hydrolytic removal of the lipid moiety gave a soluble product with higher integration activity than that of the original MPIase. This soluble form of MPIase directly interacts with a newborn membrane protein, maintaining its integration-competent structure, and allowing its post-translational integration into membranes. Thus, MPIase possesses integration-dedicated chaperone-like activity toward membrane proteins
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] MPIase is a glycolipozyme essential for membrane protein integration2012
Author(s)
Nishiyama, K., Maeda,M., Yanagisawa,K., Nagase, R., Komura, H., Iwashita, T., Yamagaki, T., Kusumoto, S., Tokuda, H., Shimamoto, K.
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Journal Title
Nat. Commun
Volume: 3
Issue: 1
Pages: 1-10
DOI
NAID
Related Report
Peer Reviewed
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