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Function and structure of novel glycolipid essential for membrane protein integration

Research Project

Project/Area Number 22310142
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionSuntory Foundation for Life Sciences

Principal Investigator

SHIMAMOTO Keiko  公益財団法人サントリー生命科学財団, 生物有機科学研究所, 主幹研究員 (70235638)

Co-Investigator(Renkei-kenkyūsha) NISHIYAMA Ken-ichi  岩手大学, 農学部, 教授 (80291334)
MIURA Kaoru (NOMURA Kaoru)  公益財団法人サントリー生命科学財団, 生物有機科学研究所, 研究員 (90353515)
KUSUMOTO Shoichi  公益財団法人サントリー生命科学財団, 生物有機科学研究所, 元所長 (30028253)
Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Keywords糖脂質 / 膜タンパク質 / 糖鎖 / 生体膜 / 膜挿入 / 構造決定 / 有機合成 / 生理活性物質 / 酵素 / シャペロン / 生理活性機構 / 生活性物質
Research Abstract

We have found a novel integration factor in the inner membrane of E. coli, naming it MPIase (membrane protein integrase). MPIase turned out to be a glycolipid composed of diacylglycerol and a glycan chain of three acetylated aminosugars linked through pyrophosphate. Hydrolytic removal of the lipid moiety gave a soluble product with higher integration activity than that of the original MPIase. This soluble form of MPIase directly interacts with a newborn membrane protein, maintaining its integration-competent structure, and allowing its post-translational integration into membranes. Thus, MPIase possesses integration-dedicated chaperone-like activity toward membrane proteins

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (23 results)

All 2013 2012 2011 2010 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (16 results) (of which Invited: 2 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] MPIase is a glycolipozyme essential for membrane protein integration2012

    • Author(s)
      Nishiyama, K., Maeda,M., Yanagisawa,K., Nagase, R., Komura, H., Iwashita, T., Yamagaki, T., Kusumoto, S., Tokuda, H., Shimamoto, K.
    • Journal Title

      Nat. Commun

      Volume: 3 Issue: 1 Pages: 1-10

    • DOI

      10.1038/ncomms2267

    • NAID

      120007179832

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] MPIase is a glycolipozyme essential for membrane protein integration2012

    • Author(s)
      Nishiyama K
    • Journal Title

      Nature Communications

      Volume: 3 Pages: 1260-1260

    • NAID

      120007179832

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel complete reconstruction system for membrane integration of the simplest membrane protein2010

    • Author(s)
      Nishiyama K., Maeda M., Abe M., Kanamori T, himamoto K., Kusumoto S., Ueda T., and Tokuda H
    • Journal Title

      Biochem.Biophys. Res. Commun

      Volume: 394 Pages: 733-736

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel complete reconstruction system for membrane integration of the simplest membrane protein2010

    • Author(s)
      Nishiyama K
    • Journal Title

      Biochem.Biophys.Res.Commun.

      Volume: 394 Pages: 733-736

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 膜タンパク質挿入の鍵を握る糖脂質2013

    • Author(s)
      島本啓子
    • Organizer
      未来を拓く有機化学 -新方法論と新戦略の創成-
    • Place of Presentation
      神戸
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 大 腸菌膜タンパク質挿入に関わる新しい因 子:機能と構造」2012

    • Author(s)
      島本、前田、永瀬、楠本、 西山
    • Organizer
      新規素材探索研究会
    • Place of Presentation
      横浜
    • Year and Date
      2012-06-08
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸菌膜タンパク質挿入に関わる新 規糖脂質MPIase の合成研究2012

    • Author(s)
      永瀬、島本、 前田、楠本、西山
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      横浜
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸菌膜タンパク質挿入に関わる新 しい因子MPIase の機能と構造2012

    • Author(s)
      島本、前田、永 瀬、柳澤、徳田、西山、楠本
    • Organizer
      第31回 日本糖質学会年会
    • Place of Presentation
      鹿児島
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸菌膜タンパク質挿入に関わる新しい因子MPIaseの機能と構造2012

    • Author(s)
      島本啓子
    • Organizer
      第31回 日本糖質学会年会
    • Place of Presentation
      鹿児島
    • Related Report
      2012 Annual Research Report
  • [Presentation] Structure and Function of Novel Glycolipid Characterized as Membrane Protein Integrase2012

    • Author(s)
      島本啓子
    • Organizer
      科研費新学術領域「天然物ケミカルバイオロジー:分子標的と活性制御」
    • Place of Presentation
      京都
    • Related Report
      2012 Annual Research Report
  • [Presentation] 大腸菌膜タンパク質挿入に関わる新規糖脂質MPIaseの合成研究2012

    • Author(s)
      永瀬良平
    • Organizer
      日本化学会第92春季年会
    • Place of Presentation
      慶応大学横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] 大腸菌膜タンパク質挿入に関わる新しい因子:機能と構造2011

    • Author(s)
      島本、前田、永瀬、楠本、西山
    • Organizer
      第14回 生命化学研究会~ in cellinteractions を調べる・動かす・くみ上げる
    • Place of Presentation
      和歌山
    • Year and Date
      2011-12-03
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸菌膜タンパク質挿入に関わる新しい因子:機能と構造2011

    • Author(s)
      島本啓子
    • Organizer
      第14回生命化学研究会~in cell interactionsを調べる・動かす・くみ上げる~
    • Place of Presentation
      ラフォーレ白浜和歌山(招待講演)
    • Year and Date
      2011-12-03
    • Related Report
      2011 Annual Research Report
  • [Presentation] 膜タンパク質挿入に関わる新規糖脂質の機能と構造2011

    • Author(s)
      前田、西山、永瀬、 岩下、小村、徳田、楠本、島本 要
    • Organizer
      第53回天然有機化合物討論会
    • Place of Presentation
      大阪
    • Related Report
      2012 Final Research Report
  • [Presentation] タンパク質膜挿入に関与する新規複合糖脂質MPIase の構造と機能2011

    • Author(s)
      西山、前田、島本、楠本、徳田
    • Organizer
      第84回 日本生化学会大会
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 膜タンパク質挿入に関わる新規糖脂質の機能と構造2011

    • Author(s)
      前田将秀
    • Organizer
      第53回天然有機化合物討論会
    • Place of Presentation
      大阪国際交流センター大阪
    • Related Report
      2011 Annual Research Report
  • [Presentation] タンパク質膜挿入に関与する新規複合糖脂質MPIaseの構造と機能2011

    • Author(s)
      西山賢一
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都国際会議場京都
    • Related Report
      2011 Annual Research Report
  • [Presentation] Structure and Function of Novel Glycolipid Characterized as Membrane Protein 科研費新学術領域「天然物ケミカルバイオロジー:分子標的と活性制御」

    • Author(s)
      島本、前田、永瀬、柳澤、徳田、西山、楠本
    • Organizer
      第1回国際領域シンポジウム
    • Place of Presentation
      京都
    • Related Report
      2012 Final Research Report
  • [Presentation] 「膜タンパク質挿入の鍵を握る糖脂質」

    • Author(s)
      島本
    • Organizer
      未来を拓く有機化学 -新方法論と新戦略の創成-
    • Place of Presentation
      神戸
    • Related Report
      2012 Final Research Report
  • [Presentation] 大腸菌膜タンパク質挿入に関わる新しい因子:機能と構造

    • Author(s)
      島本啓子
    • Organizer
      新規素材探索研究会
    • Place of Presentation
      横浜
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://www.sunbor.or.jp

    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 大腸菌膜タンパク質挿入因子2011

    • Inventor(s)
      島本啓子、楠本正一、前田将秀、西山賢一、徳田元、上田卓也、金森崇
    • Industrial Property Rights Holder
      公益財団法人サントリー生命科学財団
    • Filing Date
      2011-03-04
    • Related Report
      2012 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 大腸菌膜タンパク質挿入因子2011

    • Inventor(s)
      島本啓子, 他6名
    • Industrial Property Rights Holder
      公益財団法人サントリー生命科学財団
    • Filing Date
      2011-03-04
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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